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PSI Notebook in PDF format
PSI Notebook in PDF format

... Transcription is the process by which RNA strands are synthesized from DNA strands. This is the first step in the transport of the genetic information contained in DNA. Transcribe means to write out or rewrite, you can remember that the process of making RNA from DNA is called transcription because ...
AG-PSB-02.441-09.2 DNA-RNA
AG-PSB-02.441-09.2 DNA-RNA

... These bases will occur in sets of three called a codon. The order of a codon is specific, and the sequence of the bases determines what is being made. For example, hair color will have its own codon sequence. Explain that the nitrous bases will only combine to a certain other base. For example, Ade ...
Dismantling the Maryland DNA Convicted Offender Database
Dismantling the Maryland DNA Convicted Offender Database

... always follow certain rules: C always bond with G; T only bonds with A These are called base pairings ...
PCR amplification of the bacterial genes coding for nucleic acid
PCR amplification of the bacterial genes coding for nucleic acid

... Take following stock solutions out of the freezer and slowly thaw the frozen contents before starting with the PCR mixes One forward (fw) and one reverse (rv) primer stock solution (5μM each)  the primer pairs in use anneal with highly conserved regions of the Citrobacter freundii (Cf) 16S-rRNA gen ...
Blank notes - local.brookings.k12.sd.us
Blank notes - local.brookings.k12.sd.us

... (hyperlinks may have been added) ...
Chapter 1 The Framework of Biology
Chapter 1 The Framework of Biology

... The genetic material of prokaryotes is in the form of a circular DNA along with smaller pieces called plasmids. Many prokaryotes have flagella which propel them through their environment. Some prokaryotes have a slimy sheath on the outside of their capsule. Being small solves many problems. Gas diff ...
Chapter 19: Viruses 1. Viral Structure & Reproduction What exactly is a Virus?
Chapter 19: Viruses 1. Viral Structure & Reproduction What exactly is a Virus?

... maintain homeostasis in any way **It’s hard to “kill” something that’s not really alive, so antibiotics that kill bacteria, fungi, etc, do NOT harm viruses** ...
PROTEIN SYNTHESIS
PROTEIN SYNTHESIS

... – Converting info in sequence of mRNA bases into sequence of amino acids in protein – Takes place at ribosomes in cytoplasm ...
Recombinant DNA
Recombinant DNA

... The gene and protein of interest are isolated from the bacteria. ...
Custom-made Thermo Scientific Nunc Immobilizer for DNA Binding
Custom-made Thermo Scientific Nunc Immobilizer for DNA Binding

fragments
fragments

... The gene and protein of interest are isolated from the bacteria. ...
Unit 12 Handout - Chavis Biology
Unit 12 Handout - Chavis Biology

The Chemical Nature of DNA - RIT
The Chemical Nature of DNA - RIT

Total genomic DNA of non-treated and DHPA
Total genomic DNA of non-treated and DHPA

... respectively. GMC GT500-L DNA standard (Genomac; orange peaks) was added to fluorescently labeled products of selective amplification (green peaks) and after denaturation, ss DNA fragments were separated using ABI Prism 3100 Genetic Analyzer. Obtained data were aligned and visualized using GeneMarke ...
Background Information” DNA and gel electrophoresis
Background Information” DNA and gel electrophoresis

... called nucleotides. a. Nucleotides are made up of a 5-carbon sugar (deoxyribose) a phosphate group and one of four nitrogen bases. The bases use letters to shorten their names. 1. A is for adenine 2. G is for guanine 3. C is for cytosine 4. T is for thymine b. Purine Bases -- Adenine and guanine are ...
Handout
Handout

... What Mendel proved was that the traits are produced by factors (genes) that pass from one generation to the other Furthermore, the factors that control inheritance were not changed by their transmission Factors segregated in predictable patterns It was inferred that variability was due to the segreg ...
1) - life.illinois.edu
1) - life.illinois.edu

... the sequence. These sites are thought to be sites where IntDOT binds to cleave the DNA during strand exchange. A second class of sites, called arm sites, are labeled as R1, R2, R2’, L1 and L2 in boxes in the sequence. These sites are thought to be bound by IntDOT to form nucleoprotein complexes requ ...
T - Āris Kaksis Riga Stradin`s University assistant professor
T - Āris Kaksis Riga Stradin`s University assistant professor

... DNA copy synthesis process of replication begins, that each new cell in division process would get original parent DNA copy. Replication enzymes read nucleotide original sequences and copy over information to two new DNA molecules, which receive each divided cell as original copy. Segment of DNA mol ...
NOTES AND PROBLEM SET 3
NOTES AND PROBLEM SET 3

... • Write the Hamiltonian in terms of spatial Fourier modes (you’d better use periodic boundary conditions) and find the mean square values of mode amplitudes. • Calculate average projected length hLk i as a function of force f . Notice that hLk i is the same as extension x in the Problem 2 (this set) ...
- Ms. Ottolini`s Biology Wiki!
- Ms. Ottolini`s Biology Wiki!

... 18. Modification #1: GTP “cap” (guanine added to 5’ end of RNA to prevent degradation and enable the mRNA to bind to the ribosome during translation) 19. Modification #2: PolyA “tail” (AAA added to the 3’ end to prevent degradation and enable the mRNA to pass through the pores in the nuclear membran ...
Freshman Seminar
Freshman Seminar

Chapter 1 [4Fe-4S] Cluster Base Excision Repair Glycosylases
Chapter 1 [4Fe-4S] Cluster Base Excision Repair Glycosylases

Genetics
Genetics

... 129. Distinguish between the members of each of the following pairs of terms, by writing a sentence about each member of each pair. (i) Gene and allele. (ii) Homozygous and heterozygous. (iii) Genotype and phenotype. (iv) Linkage and sex linkage. 130. In genetics, what is meant by the term variation ...
Genetics Assessment
Genetics Assessment

... other organisms? Today you will perform a transformation using a paper model. What is a transformation? Bacteria have an extra piece of DNA that is much smaller than the rest of their genome, called a plasmid. This DNA is double-stranded and in the form of a circle. Transformation occurs when a gene ...
Supplementary Material and Methods
Supplementary Material and Methods

... Minneapolis, MM, USA). Each sample was divided into two parts and a cDNA synthesis was ...
< 1 ... 152 153 154 155 156 157 158 159 160 ... 657 >

Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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