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Teacher Guide - the BIOTECH Project
Teacher Guide - the BIOTECH Project

... negatively charged, so to move the DNA into the gel with electricity, the DNA needs to be loaded on the negative or black side, it will then move towards the red. If it's loaded near the red electrode, it will migrate off the gel. Draw a picture of your gel and label which samples are where before y ...
Electrophoretic stretching of DNA molecules using microscale T
Electrophoretic stretching of DNA molecules using microscale T

... depending on the length of the DNA relative to the channel width in the junction region. Stable trapping and stretching of DNA molecules up to lengths of 485 kbp are demonstrated. © 2007 American Institute of Physics. 关DOI: 10.1063/1.2745650兴 The ability to trap and stretch biopolymers is important ...
TG_REV_NAP_short.ver2 - RI
TG_REV_NAP_short.ver2 - RI

...  The molecular concept of hydrophobicity is complex, and quite important. Students often think hydrophobic molecules actively avoid water. In fact, at the molecular level, hydrophobicity is the result of water molecules being more strongly attracted to each other, due to their polarity, than to hyd ...
Table 3.1. List of suppliers of restriction enzymes. Name of
Table 3.1. List of suppliers of restriction enzymes. Name of

The Function of the Hereditary Materials - Bruce Alberts
The Function of the Hereditary Materials - Bruce Alberts

... and studied in isolation from the rest of the ribosomal RNA transcript (Cech et al, 1983; Bass and Cech, 1984). This sequence folds up to form a complex surface that functions like an enzyme in several reactions. For example, it can bind two specific substrates tightly (Km — 10~5 M)—a guanine nucleo ...
Central Dogma Mini-Book Instructions
Central Dogma Mini-Book Instructions

... bring amino acids to the mRNA, they read the mRNA code in sections of 3 nucleotides at a time (codons) and use this code to build amino acid chains. Each group of tRNA molecules get a different piece of mRNA which contains different directions, so that each can build a different kind of protein. The ...
Define genetics, genome, chromosome, gene, genetic code
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... Describe the process of DNA replication. Describe protein synthesis, including transcription, RNA processing, and translation. Classify mutations by type, and describe how mutations are prevented and repaired. ...
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... In prokaryotes, the methionine on the initiating methionyl tRNA is formylated (fmet-tRNA). In eukaryotes, it is not. Not every AUG codon can be an initiation codon. Sequences in the mRNA leader seem to indicate which AUG codons are initiation codons. The initial methionine may be removed after trans ...
Gene Isolation and Manipulation
Gene Isolation and Manipulation

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three possibile models for replication

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Bio212-01-Alu Lab Part1
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... us carry approximately 500,000 copies of a 300 bp sequence known as the Alu sequence in our DNA. The origin and function of these sequences are still unknown. Despite this, these repeated Alu sequences have proved interesting for geneticists as when they are present in particular genes, they can be ...
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SuperScript Choice cDNA synthesis

... the larger fragments down to the desired size range for library construction. If the samples are to be sonicated the following day, store tubes in 6th floor library construction room 20C freezer in the appropriate box. 3.16. If there is sufficient time to sonicate sample(s) on the same day, follow ...
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Chapter 17 Notes
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... Three properties of RNA allow some RNA molecules to function as ribozymes. 1. Because RNA is single-stranded, a region of the RNA molecule may base-pair with a complementary region elsewhere in the same molecule, giving the RNA a specific threedimensional structure that is key to its ability to cata ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

SAM Teachers Guide Nucleic Acids and Proteins - RI
SAM Teachers Guide Nucleic Acids and Proteins - RI

... • The molecular concept of hydrophobicity is complex, and quite important.  Students often think hydrophobic molecules actively avoid water. In fact,  at the molecular level, hydrophobicity is the result of water molecules  being more strongly attracted to each other, due to their polarity, than to  ...
DNA Fingerprinting
DNA Fingerprinting

... The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNKA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a tumor suppressor genes such as one called p53. These genes usually keep cells from div ...
DNA Transcription and Translation - MrsGorukhomework
DNA Transcription and Translation - MrsGorukhomework

... slides along the mRNA until it reaches the start codon – AUG. tRNA and the large subunit are brought together. The anticodon of the tRNA joins with the complementary base pair of the codon of the mRNA. The tRNA anticodon first joins with the start codon on the mRNA and forms a hydrogen bond between ...
DNA App Notes
DNA App Notes

Essential Cell Biology chapter 5 excerpt
Essential Cell Biology chapter 5 excerpt

... with E. coli; after allowing a few minutes for infection to take place, they poured the mix into a Waring blender and hit “puree.” The blender’s spinning blades sheared the empty virus heads from the surfaces of the bacterial cells. The researchers then centrifuged the sample to separate the heavier ...
Chapter 14 Biotechnology and Genomics
Chapter 14 Biotechnology and Genomics

... viral reproduction by cleaving viral DNA. •The restriction enzyme is used to cut DNA at specific points during production of rDNA. •It is called a restriction enzyme because it restricts growth of viruses but it acts as a molecular scissors to cleave any piece of DNA at a specific site. •Restriction ...
Biology Standard 2 Test Prep
Biology Standard 2 Test Prep

... B. one time C. two times D. four times 23. What is (are) formed during replication? A. amino acids B. DNA C. protein D. RNA 24. Hemophilia is more common in males than females because it is caused by a A. dominant gene found on the X chromosome. B. dominant gene found on the Y chromosome. C. recessi ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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