Reporter genes
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
Macromolecules
... Note that no break in the polymer chain (disruption of primary structure) is involved in denaturation. Denaturing agents disrupt stabilizing factors. Agents that disrupt hydrogen bonding: Heat -- thermal agitation (vibration, etc.) -- will denature proteins or nucleic acids. Heat denaturation of DNA ...
... Note that no break in the polymer chain (disruption of primary structure) is involved in denaturation. Denaturing agents disrupt stabilizing factors. Agents that disrupt hydrogen bonding: Heat -- thermal agitation (vibration, etc.) -- will denature proteins or nucleic acids. Heat denaturation of DNA ...
Name of Student: Dominik Sommerfeld
... the human genome are subjected to phosphorylation on multiple sites, and there may be in excess of 500,000 phosphorylation sites (P-sites) in the human proteome. To ensure signalling fidelity amidst the vast complement of potential substrate P-sites, protein kinases must exert specificity for their ...
... the human genome are subjected to phosphorylation on multiple sites, and there may be in excess of 500,000 phosphorylation sites (P-sites) in the human proteome. To ensure signalling fidelity amidst the vast complement of potential substrate P-sites, protein kinases must exert specificity for their ...
emboj200852-sup
... RT-PCR fragment in pCDNA3 after Bgl2-EcoRI restriction digest. The muscle specific expression plasmid for eIF3-f was carrying out first by using the 1256 bp HindIII-BstEII filled-in fragment of the muscle regulatory elements of Muscle Creatine Kinase (MCK) and subcloned in pEGFP-C1 instead of the C ...
... RT-PCR fragment in pCDNA3 after Bgl2-EcoRI restriction digest. The muscle specific expression plasmid for eIF3-f was carrying out first by using the 1256 bp HindIII-BstEII filled-in fragment of the muscle regulatory elements of Muscle Creatine Kinase (MCK) and subcloned in pEGFP-C1 instead of the C ...
Diffusion Demonstration
... Osmosis: when water moves across the plasma membrane by passive transport ...
... Osmosis: when water moves across the plasma membrane by passive transport ...
GFP
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
Protein Metabolism and Storage with Special Consideration of the
... but there are different storage molecules with different storage capacities. The body can thus store protein in the form of collagen, while glucose, amino acids, fatty acids and water are stored in mucopolysaccharides. Fat and glucose are stored in fat cells in the form of triglycerides, and finally ...
... but there are different storage molecules with different storage capacities. The body can thus store protein in the form of collagen, while glucose, amino acids, fatty acids and water are stored in mucopolysaccharides. Fat and glucose are stored in fat cells in the form of triglycerides, and finally ...
Chapter 09 Lecture PowerPoint - McGraw Hill Higher Education
... different hydrogenbonding profiles to amino acids approaching either major or minor groove ...
... different hydrogenbonding profiles to amino acids approaching either major or minor groove ...
SpeeDB: fast structural protein searches
... and regulate chemical reactions. Some proteins transport and store essential biological molecules such as oxygen, metal ions and glucose. Other proteins are found in connective tissues and function as structural elements; collagen is an example of a structural protein which occurs in all multicellul ...
... and regulate chemical reactions. Some proteins transport and store essential biological molecules such as oxygen, metal ions and glucose. Other proteins are found in connective tissues and function as structural elements; collagen is an example of a structural protein which occurs in all multicellul ...
Evolution of the Bacterial Flagellum
... Finally, expression of flagellar genes involves transcription using a flagellumspecific sigma factor to initiate mRNA synthesis by RNA polymerase. This sigma factor (F or FliA) is homologous to many other sigma factors in bacteria and could have evolved from a primordial vegetative . ...
... Finally, expression of flagellar genes involves transcription using a flagellumspecific sigma factor to initiate mRNA synthesis by RNA polymerase. This sigma factor (F or FliA) is homologous to many other sigma factors in bacteria and could have evolved from a primordial vegetative . ...
viewpoint - Lindquist Lab
... further cemented the view that proteins are simply the active incarnation of information stored in the DNA, and rang the death bell for the template theory of antibody formation (Haurowitz, 1950). But the idea that a protein could transmit information did not disappear. Carleton Gajdusek had already ...
... further cemented the view that proteins are simply the active incarnation of information stored in the DNA, and rang the death bell for the template theory of antibody formation (Haurowitz, 1950). But the idea that a protein could transmit information did not disappear. Carleton Gajdusek had already ...
Lecture 9 - Screening cDNA libraries (AMG text pp. 128-134)
... What protein function cannot be present in the bait protein, i.e., what function would give high background? Why would you want to have both the Gal4 dependent His3 and LacZ reporter genes in the host yeast strain? Which is a genetic selection and which is a genetic screen? cDNA Phage Display librar ...
... What protein function cannot be present in the bait protein, i.e., what function would give high background? Why would you want to have both the Gal4 dependent His3 and LacZ reporter genes in the host yeast strain? Which is a genetic selection and which is a genetic screen? cDNA Phage Display librar ...
Receptor-drug interaction
... Macromolecules such as: 1. Proteins (mainly enzymes, receptors and transport proteins) 2. Lipids 3. Carbohydrates 4. Nucleic acids (DNA and RNA) are the main molecular targets for drugs. For a drug to have an action, it should interact [bind] with one of these molecules. The area of the macromolecul ...
... Macromolecules such as: 1. Proteins (mainly enzymes, receptors and transport proteins) 2. Lipids 3. Carbohydrates 4. Nucleic acids (DNA and RNA) are the main molecular targets for drugs. For a drug to have an action, it should interact [bind] with one of these molecules. The area of the macromolecul ...
Supplemental Data
... faster on native gels than Gal80p by itself. Hence, a mixture between both proteins gives rise to three dimer combinations with distinct mobilities: a slowly migrating Gal80p homodimer, a fast migrating Gal80pVP16 homodimer, and a Gal80p/Gal80pVP16 heterodimer with intermediate mobility (LEUTHER 199 ...
... faster on native gels than Gal80p by itself. Hence, a mixture between both proteins gives rise to three dimer combinations with distinct mobilities: a slowly migrating Gal80p homodimer, a fast migrating Gal80pVP16 homodimer, and a Gal80p/Gal80pVP16 heterodimer with intermediate mobility (LEUTHER 199 ...
PowerPoint
... – Van der Waals forces (transient, weak electrical attraction of one atom for another) – Hydrophobic (clustering of nonpolar groups) – Hydrogen bonding ...
... – Van der Waals forces (transient, weak electrical attraction of one atom for another) – Hydrophobic (clustering of nonpolar groups) – Hydrogen bonding ...
Instructions for FUEL-mLoc Web-server
... vectors with dimensionality of more than eight thousand. By using the one-vs-rest ENbased (elastic net-based) classifiers, much fewer GO terms are selected. Subsequently, the dimension-reduced feature vectors are classified by a multi-label EN classifier. Based on the selected essential GO terms, th ...
... vectors with dimensionality of more than eight thousand. By using the one-vs-rest ENbased (elastic net-based) classifiers, much fewer GO terms are selected. Subsequently, the dimension-reduced feature vectors are classified by a multi-label EN classifier. Based on the selected essential GO terms, th ...
Replacing Traditional SDS-PAGE and Bradford Techniques
... The 5100 ALP, using the Protein 200 HT-2 assay, was used to analyze all fractions from a purification process in a faster and more automated way. A major advantage is that the 5100 ALP provides both protein concentration and purity simultaneously, eliminating the need for a Bradford assay and SDS PA ...
... The 5100 ALP, using the Protein 200 HT-2 assay, was used to analyze all fractions from a purification process in a faster and more automated way. A major advantage is that the 5100 ALP provides both protein concentration and purity simultaneously, eliminating the need for a Bradford assay and SDS PA ...
Text S1.
... presented later in this paper indicate clearly that the number in the second place of the hydropathy values is of little consequence to the hydropathy profiles, and as a result we did not hesitate to adjust the values subjectively when only this level of accuracy was in question. Nevertheless, we tr ...
... presented later in this paper indicate clearly that the number in the second place of the hydropathy values is of little consequence to the hydropathy profiles, and as a result we did not hesitate to adjust the values subjectively when only this level of accuracy was in question. Nevertheless, we tr ...
Chemical Messengers
... • G proteins can either be stimulatory or inhibitory. • Once the alpha subunit of the G protein activates its effector protein, a GTP-ase activity inherent in the alpha subunit cleaves the GTP into GDP plus Pi. • This cleavage renders the alpha subunit inactive, allowing it to recombine with its bet ...
... • G proteins can either be stimulatory or inhibitory. • Once the alpha subunit of the G protein activates its effector protein, a GTP-ase activity inherent in the alpha subunit cleaves the GTP into GDP plus Pi. • This cleavage renders the alpha subunit inactive, allowing it to recombine with its bet ...
DNA and RNA
... • tRNA is a strand of RNA nucleotides, what is found at each of its ends? • Wobble position=Not Needed • Joining of amino acid to the tRNA is performed by what type of molecule? (Specific name is not needed) ...
... • tRNA is a strand of RNA nucleotides, what is found at each of its ends? • Wobble position=Not Needed • Joining of amino acid to the tRNA is performed by what type of molecule? (Specific name is not needed) ...
Carbohydrates (CHO) - Ms. Karellas
... chemical or physical methods, elements consist of only one type of atom, an atom is the smallest component of an element that retains the properties of that element A compound is a pure substance composed of two or more elements chemically combined, there is a specific ratio of types of atoms Atoms ...
... chemical or physical methods, elements consist of only one type of atom, an atom is the smallest component of an element that retains the properties of that element A compound is a pure substance composed of two or more elements chemically combined, there is a specific ratio of types of atoms Atoms ...
Lipids
... a polypeptide chain is formed. A protein consists of one or more polypeptide chains folded into a highly specific 3D shape. There are up to four levels of structure in a protein: primary, secondary, tertiary and quaternary. Each of these play an important role in the overall structure and function o ...
... a polypeptide chain is formed. A protein consists of one or more polypeptide chains folded into a highly specific 3D shape. There are up to four levels of structure in a protein: primary, secondary, tertiary and quaternary. Each of these play an important role in the overall structure and function o ...
Protein adsorption
Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.