• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Dysregulation of Purine Nucleotide Biosynthesis Pathways
Dysregulation of Purine Nucleotide Biosynthesis Pathways

... the 3⬘GFP-BamHI primer and created a frame shift-containing construct (pfcy2mg). The GFP fusion constructs were overexpressed in the wild-type (BY4741) and fcy2⌬ strains under the induction of galactose and monitored using a fluorescence microscope. Plasmids used for overexpression of ADE genes were ...
An overview of biochemistry for bioCHEM480
An overview of biochemistry for bioCHEM480

... A  successful  organism  has  to  be  able  to  synthesize  compounds  not  supplied  by  the  diet  in  order  to   survive.  This  includes  compounds  of  high  chemical  potential  energy  (i.e.  ATP)  for  both  growth,   developme ...
Diagnosing Diabetes Mellitus in Adults Part 3
Diagnosing Diabetes Mellitus in Adults Part 3

... Zhao M1, Wang Z1, Yung S2, Lu Q. Understanding type 2 diabetes: from genetics to epigenetics. Raciti GA, Longo M, Parrillo L, Ciccarelli M, Mirra P, Ungaro P, Formisano P, Miele C, Béguinot F. Acta Diabetol. 2015 Apr 5. ...
Use of Gene-Specific Primer Cocktails for First
Use of Gene-Specific Primer Cocktails for First

... in which a handful of genes are examined, especially synthesis was primed with either oligo-(dT), random for multiple rare transcripts. hexamer, or a cocktail of 10 gene-specific antisense primers. Using 0.5 µL of each resulting cDNA reacThe authors examined the expression of 10 genes in tion as a t ...
file - UCL Discovery
file - UCL Discovery

Chapter 5 – Extensions and Modifications of Basic Principles
Chapter 5 – Extensions and Modifications of Basic Principles

... – Some extra digits are fully functional; others are just small skin tags ...
From essential to persistent genes: a functional
From essential to persistent genes: a functional

... a pathogen. These studies were carried out on libraries of microorganisms using transposons or antisense RNA expression [4], but the ‘minimal set’ outcomes differed widely in terms of gene number (and often identity), not only in distant organisms but also in the same organism under different (and s ...
Exercise- Genetics 1. Which of the following statements is true of
Exercise- Genetics 1. Which of the following statements is true of

... C. guanine(G) D. thymine(T) ...
Seed specific polycomb group gene and methods of use for same
Seed specific polycomb group gene and methods of use for same

... mother cell, and 3) adventitious embryony-embryo develops directly from a somatic cell. In most forms of apomixis, pseudogamy or fertiliZation of the polar nuclei to produce endosperm is necessary for seed viability. These types of apomixis have economic potential because they can cause any genotype ...
GUEST COMMENTARY
GUEST COMMENTARY

... generated trp-lac fusions (4). These deletions and trp-lac fusions were used extensively to help elucidate operon structure and detailed regulatory mechanisms for both trp and lac (for example, see the chapters by Beckwith, Miller, and Bassford et al. in reference 24). This work clearly demonstrated ...
Slide 1
Slide 1

... * is present on the 5' end of the 5' to 3' strand. Thus when copying * a 5' to 3' strand, the resulting 3' to 5' strand is always the same length * as the template strand. However, when replicating a 3' to 5' strand, * the resulting 5' to 3' strand is one unit shorter than the template. The * missin ...
file
file

... category within clusters • Each gene was mapped into one of 199 functional categories ( according to MIPS database ). • For each cluster, P-values was calculated for observing the frequencies of genes from particular functional categories. ...
genotyping single nucleotide polymorphisms located on
genotyping single nucleotide polymorphisms located on

... SNPs. The speed and accuracy of MALDI-TOF MS also allows rapid development of large DNA typing databases and population studies. Because SNPs are typically bi-alleic, a greater number of these markers are needed in comparison to short tandem repeat (STR) markers for human identification purposes. Th ...
Contrasting Effects of ENU Induced Embryonic Lethal Mutations of
Contrasting Effects of ENU Induced Embryonic Lethal Mutations of

... Product size is 349 bp. N, as Table 2; GAVA R2, as Table 2, PCR conditions e (Table 2). Product size is 388 bp. N, as Table 2; GAVA R1, GTA GTG ACT AAA CAA ACA GCA TGT C, PCR conditions e (Table 2). Product size is 832 bp. 490F, AGC TGC GGA GCC TGG AAT AT; 1033R, TCA CTT CTT CAA CCG CTC TC, PCR cond ...
Ch 5 Macromolecules
Ch 5 Macromolecules

... the dramatic effect on the protein function that occurs when valine is substituted for glutamic acid. The R group on glutamic acid is acidic and hydrophilic, wheras the R group on valine is nonpolar and hydrophibic. Therefore, it is unlikely that valine can participate in the same intramolecular int ...
6SULQJHU
6SULQJHU

... appropriate restriction enzymes and subcloned into pBS-KS (Stratagene). Using these constructs as templates, further PCRs were carried out to generate cDNAs encoding the receiver module (aa 1 to aa 164) and the C-terminal domain of ARR2 (aa 165 to aa 664). To isolate the ARR2 promoter (nucleotide po ...
Remarkably Little Variation in Proteins Encoded
Remarkably Little Variation in Proteins Encoded

... data, the relative proportion of nonsynonymous to synonymous variant sites is 0.24 (1/1601 versus 1/378; Table 3), and the relative proportion of nonsynonymous to intronic variant sites is 0.39 (1/1601 versus 1/630). Roughly analogous values for a collection of 75 non-Y-linked human genes are simila ...
Basic Array Analysis
Basic Array Analysis

... Instead, we may use some measure of the overall response of the sample to normalize. For example, we may compute the mean or median value across analytes for each sample (Mi) and the overall mean or median M of the Mi across samples, and then normalize the value yij for analyte j from sample i to yi ...
cerebellar abiotrophy test report cba1110 cerebellar abiotrophy result
cerebellar abiotrophy test report cba1110 cerebellar abiotrophy result

... Cerebellar Abiotrophy (CA) is a neurological genetic disease found in Arabian horses that is characterized by head tremor and lack of balance equilibrium. CA is inherited as a recessive trait and thus breedings between two carriers (N/CA) have a 25% chance of producing an affected foal. Breedings be ...
No Slide Title
No Slide Title

... Darwin and homology • “The natural system is based upon descent with modification .. the characters that naturalists consider as showing true affinity (i.e. homologies) are those which have been inherited from a common parent, and, in so far as all true classification is genealogical; that communit ...
Preview from Notesale.co.uk Page 1 of 1
Preview from Notesale.co.uk Page 1 of 1

... Genetic Diagrams. Remember you have two genes for each characteristic and different versions of the same gene are called alleles. ...
Unit 6
Unit 6

... scientific basis for the genetic superiority of one race over any other one; and it creates the first genetic map of the world. In the process of collecting information for the book, however, scientists encountered great difficulties. The ultimate goal of their work is to remove racial prejudice. ...
Organic and Inorganic Molecules - Cal State LA
Organic and Inorganic Molecules - Cal State LA

... Acidic amino acids: R group contains a carboxyl (-COOH) group (example: aspartic acid, glutamic acid) Basic amino acids: R group contains an amino group or nitrogen-containing group (example: lysine, histidine) Polar amino acids: R group contains lots of hydroxyl groups (-OH; very soluble in water) ...
EOC Review Questions
EOC Review Questions

... a. The cells ability to reproduce because it could not make any ATP b. The cells ability to make proteins because there would be no ribosomes. c. the cells ability to produce energy because the vacuoles could no longer contract. d. The cells ability to produce energy because the mitochondria could n ...
Genetic Regulatory Network Identification Using
Genetic Regulatory Network Identification Using

< 1 ... 918 919 920 921 922 923 924 925 926 ... 2254 >

Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report