Expression and characterization of von Willebrand
... plasmids were cloned into Epicurian XL1-Blue MRF⬘ Kan. Plasmids were purified by using the Endofree Plasmid Maxi Kit (Qiagen, Hilden, Germany). COS-7 cells (4 ⫻ 105; DSMZ, Braunschweig, Germany) were transiently transfected with mutant and WT fragment II DNA (10 g each) by using the Lipofectamine P ...
... plasmids were cloned into Epicurian XL1-Blue MRF⬘ Kan. Plasmids were purified by using the Endofree Plasmid Maxi Kit (Qiagen, Hilden, Germany). COS-7 cells (4 ⫻ 105; DSMZ, Braunschweig, Germany) were transiently transfected with mutant and WT fragment II DNA (10 g each) by using the Lipofectamine P ...
Creating order from chaos: epigenome dynamics in
... Finally, it is worth considering variation in selection against TE proliferation at the level ...
... Finally, it is worth considering variation in selection against TE proliferation at the level ...
Rat sn-glycerol-3-phosphate acyltransferase - UNC
... GPAT cDNA. The presence of the deletion was con¢rmed by sequencing the product obtained using 24S and 8AS primers in PCR ampli¢cation of rat liver cDNA. The 3P UTR was only partially sequenced but appeared to be about 84% homologous to the 3362 base mouse GPAT 3P UTR (data not shown). The nucleotide ...
... GPAT cDNA. The presence of the deletion was con¢rmed by sequencing the product obtained using 24S and 8AS primers in PCR ampli¢cation of rat liver cDNA. The 3P UTR was only partially sequenced but appeared to be about 84% homologous to the 3362 base mouse GPAT 3P UTR (data not shown). The nucleotide ...
Polyploidy
... Chromosomal Duplication ...an event that results in the increase in the number of copies of a particular chromosomal region, ...
... Chromosomal Duplication ...an event that results in the increase in the number of copies of a particular chromosomal region, ...
The genetics of Caenorhabditis elegans.
... mutations on nervous systems. I n principle, it should be possible to dissect the genetic specification of a nervous system in much the same way as was done for biosynthetic pathways in bacteria or for bacteriophage assembly. However, one surmises that genetical analysis alone would have provided on ...
... mutations on nervous systems. I n principle, it should be possible to dissect the genetic specification of a nervous system in much the same way as was done for biosynthetic pathways in bacteria or for bacteriophage assembly. However, one surmises that genetical analysis alone would have provided on ...
Daniël van Draanen - Utrecht University Repository
... traits is limited to outer visible traits like eye colour, hair colour and skin texture (WoodRobinson, Lewis, & Leach, 2000). Further findings about educational use of the terms are discussed in paragraph 3.5 of the theoretical basis. According to concept learning theories, students have a prototyp ...
... traits is limited to outer visible traits like eye colour, hair colour and skin texture (WoodRobinson, Lewis, & Leach, 2000). Further findings about educational use of the terms are discussed in paragraph 3.5 of the theoretical basis. According to concept learning theories, students have a prototyp ...
Duplication 12p and PallisterKillian syndrome
... alopecia, diaphragmatic hernia, congenital heart defects, and other systemic abnormalities. PKS is typically caused by the presence of a supernumerary isochromosome composed of the short arms of chromosome 12 resulting in tetrasomy 12p, which is often present in a tissue limited mosaic state. The PK ...
... alopecia, diaphragmatic hernia, congenital heart defects, and other systemic abnormalities. PKS is typically caused by the presence of a supernumerary isochromosome composed of the short arms of chromosome 12 resulting in tetrasomy 12p, which is often present in a tissue limited mosaic state. The PK ...
Journal of Applied Microbiology
... strains, not isolated from potato, have been described as atypical E. c. atroseptica because, although they produce acid from a-methylglucoside and reducing substances from sucrose like E. c. atrospetica, they differ in having the ability to grow at 37C. Some of these strains, isolated from several ...
... strains, not isolated from potato, have been described as atypical E. c. atroseptica because, although they produce acid from a-methylglucoside and reducing substances from sucrose like E. c. atrospetica, they differ in having the ability to grow at 37C. Some of these strains, isolated from several ...
Persistence and Loss of Meiotic Recombination
... created by mutagenesis, hotspot loci are known to be polymorphic in natural populations (Catcheside 1975; Guillon and de Massy 2002; Carrington and Cullen 2004). Furthermore, biased conversion in hotspot heterozygotes has also been detected in human and mouse meioses (Jeffreys and Neumann 2002; Yauk ...
... created by mutagenesis, hotspot loci are known to be polymorphic in natural populations (Catcheside 1975; Guillon and de Massy 2002; Carrington and Cullen 2004). Furthermore, biased conversion in hotspot heterozygotes has also been detected in human and mouse meioses (Jeffreys and Neumann 2002; Yauk ...
IBS Methods for Affected Pairs Linkage
... • Unselected pairs • Unaffected pairs • Discordant pairs ...
... • Unselected pairs • Unaffected pairs • Discordant pairs ...
Gene Section GAST (gastrin) Atlas of Genetics and Cytogenetics
... on the sequence of preprogastrin, which includes the 21 amino acids of the signal peptide sequence, whereas the numbering system of others is based on the sequence of progastrin. The numbers at the top of the diagram represents the amino acid (aa) sequence for preprogastrin; the numbers at the botto ...
... on the sequence of preprogastrin, which includes the 21 amino acids of the signal peptide sequence, whereas the numbering system of others is based on the sequence of progastrin. The numbers at the top of the diagram represents the amino acid (aa) sequence for preprogastrin; the numbers at the botto ...
Barley genetic variation: implications for crop improvement
... Barley Core, AM panels of elite breeding lines, nested association mapping (NAM) populations and wild barley-introgression populations. Accessing the diversity within the NSGC Core is one of the main goals of the TCAP, as the locally adapted landraces and historical breeding materials stored in the ...
... Barley Core, AM panels of elite breeding lines, nested association mapping (NAM) populations and wild barley-introgression populations. Accessing the diversity within the NSGC Core is one of the main goals of the TCAP, as the locally adapted landraces and historical breeding materials stored in the ...
p53 in Hematologic Malignancies
... located outside the area examined by SSCP. More than 90% of mutations of p53 probably occur in exons 5 through 8 andmostanalysishas focused onthis r e g i ~ n . ' ~ . ' " ~An ...
... located outside the area examined by SSCP. More than 90% of mutations of p53 probably occur in exons 5 through 8 andmostanalysishas focused onthis r e g i ~ n . ' ~ . ' " ~An ...
Acetate formation in the photoheterotrophic bacterium Chloroflexus
... conversion of acetyl-CoA/acetate (acetyl-CoA + ADP + Pi ↔ acetate + ATP + CoA) were determined at 55 °C, pH 7.5. In both directions of the reaction, the rate dependence on the substrate concentrations followed Michaelis–Menten kinetics. The apparent Vmax and Km values in the direction of acetate for ...
... conversion of acetyl-CoA/acetate (acetyl-CoA + ADP + Pi ↔ acetate + ATP + CoA) were determined at 55 °C, pH 7.5. In both directions of the reaction, the rate dependence on the substrate concentrations followed Michaelis–Menten kinetics. The apparent Vmax and Km values in the direction of acetate for ...
The Application of Hydrolytic Enzymes for Biotransformations of
... enzymes has been studied. A range of small to medium sized commercially available vinyl esters (C2- C10), as well as amino acid vinyl esters were selected for the biotransformations. For the esterification of naringin, small-scale reactions were carried out for 72 hrs and the reaction mixtures were ...
... enzymes has been studied. A range of small to medium sized commercially available vinyl esters (C2- C10), as well as amino acid vinyl esters were selected for the biotransformations. For the esterification of naringin, small-scale reactions were carried out for 72 hrs and the reaction mixtures were ...
Major players on the microbial stage: why archaea
... eukarya and archaea is widely accepted, the origin of the Domain Eukarya is one of the most controversial areas of evolutionary biology (Embley & Martin, 2006). Variations of two broad theories on the origin of eukaryotic cells involving the Archaea have been presented. The first posits that the Arc ...
... eukarya and archaea is widely accepted, the origin of the Domain Eukarya is one of the most controversial areas of evolutionary biology (Embley & Martin, 2006). Variations of two broad theories on the origin of eukaryotic cells involving the Archaea have been presented. The first posits that the Arc ...
PDF - Biology Direct
... features with it. The coevolution theory proposes that the first code incorporated only a small number of amino acids and that the later amino acids were added sequentially to the code. The earliest amino acids are those that are simplest to synthesize, either by prebiotic chemistry in the environme ...
... features with it. The coevolution theory proposes that the first code incorporated only a small number of amino acids and that the later amino acids were added sequentially to the code. The earliest amino acids are those that are simplest to synthesize, either by prebiotic chemistry in the environme ...
Facts About Myotonic Muscular Dystrophy
... — congenital MMD — and the type that begins in adolescence or adulthood — adultonset MMD. ...
... — congenital MMD — and the type that begins in adolescence or adulthood — adultonset MMD. ...
Identification and characterization of novel interaction
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
BiochemicalSociety A nnualSymposium No.79
... P450s are a large and diverse family of enzymes found in all biological kingdoms. They are cysteine thiolateco-ordinated haem b-containing enzymes and have a characteristic absorption band at 450 nm when the reduced haem iron binds to carbon monoxide. The most common P450 reaction is a mono-oxygenat ...
... P450s are a large and diverse family of enzymes found in all biological kingdoms. They are cysteine thiolateco-ordinated haem b-containing enzymes and have a characteristic absorption band at 450 nm when the reduced haem iron binds to carbon monoxide. The most common P450 reaction is a mono-oxygenat ...
Metabolism of bile acids
... size causing vitamin deficiencies, lipid malabsorption and liver failure [7–9]. The acidic pathway starts with the hydroxylation of the cholesterol side-chain by sterol 27-hydroxylase (CYP27). The CYP27 product, 5-cholesten-3β-27-diol, is not a substrate for CYP7A1, but is hydroxylated at the C7 pos ...
... size causing vitamin deficiencies, lipid malabsorption and liver failure [7–9]. The acidic pathway starts with the hydroxylation of the cholesterol side-chain by sterol 27-hydroxylase (CYP27). The CYP27 product, 5-cholesten-3β-27-diol, is not a substrate for CYP7A1, but is hydroxylated at the C7 pos ...
Multiple RNA regulatory elements mediate distinct
... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
Liver glucose metabolism in humans
... promoters allow the glucokinase gene to be independently regulated in liver and pancreatic β-cells. Comparison of the nucleotide sequences of the human islet glucokinase cDNA with that of the human liver glucokinase cDNA reveals that the two cDNAs differ on their 5´ends while having identical 3´-en ...
... promoters allow the glucokinase gene to be independently regulated in liver and pancreatic β-cells. Comparison of the nucleotide sequences of the human islet glucokinase cDNA with that of the human liver glucokinase cDNA reveals that the two cDNAs differ on their 5´ends while having identical 3´-en ...