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View Powerpoint Presentation - Northeast Biomanufacturing Center
View Powerpoint Presentation - Northeast Biomanufacturing Center

... Industry”, Robert McKown, and George L. Coffman, May/June 2002, ...
Energetics of protein–DNA interactions
Energetics of protein–DNA interactions

... Protein–DNA interactions are vital for many processes in living cells, especially transcriptional regulation and DNA modification. To further our understanding of these important processes on the microscopic level, it is necessary that theoretical models describe the macromolecular interaction energ ...
Use of a single primer to fluorescently label selective amplified
Use of a single primer to fluorescently label selective amplified

... resulting AFLP profiles are separated using polyacrylamide, and the markers are scored for presence or absence (5). One of the advantages of AFLP is that a large number of markers can be generated based on differential combinations of selective primers (5). The use of fluorescently labeled selective ...
S3 Text.
S3 Text.

Meiosis
Meiosis

swift.cmbi.ru.nl
swift.cmbi.ru.nl

... ©CMBI 2009 ...
The complete nucleotide sequence of cucumber green mottle
The complete nucleotide sequence of cucumber green mottle

... protein gene and the 3' non-coding region. The genome organization of CGMMV-SH was compared to three other sequenced tobamoviruses, TMV vulgare (Goelet et al., 1982), TMV-L (Ohno et al., 1984) and tobacco mild green mosaic virus (TMGMV, TMV-U2) (Solis & Garcia-Arenal, 1990) (Fig. 2), The genome of C ...
Appendix - Cengage
Appendix - Cengage

... The electrons between two atoms in a covalent bond are not always shared equally. When the atoms sharing an electron pair are identical, such as two oxygen atoms, the electrons are attracted equally by both atoms and so are shared equally. The result is a nonpolar molecule. The term nonpolar implies ...
Is structural flexibility of antigen-binding loops
Is structural flexibility of antigen-binding loops

... The computer graphic models of VH and VL chains of antidsDNA mAb were built, using the graphics program Insight II and Homology (Version 2.3.0, Biosym Technologies, San Diego, CA), on a Silicon Graphics (Indigo2) computer system. The general strategy for the model building was as follows: (i) select ...
Structural Bioinformatics
Structural Bioinformatics

... CASP Experiments ...
Intelligence: Genetics, Genes, and Genomics
Intelligence: Genetics, Genes, and Genomics

... shared environmental influence. As noted earlier, intelligence, unlike other personality traits, shows shared environmental influence. The twin data summarized in Figure 2 suggest that shared environment effects are negligible in adulthood. Data for adoptive siblings summarized in Figure 1, which pr ...
Hair follicle differentiation: expression, structure and
Hair follicle differentiation: expression, structure and

... information indicate that the IF superfamily contains more than 40 components. Several genes have been sequenced and their common structures and sequence conservation suggest that they could have evolved from a primordial gene. The present IF gene superfamily is composed of at least 6 families and a ...
BAC White Paper - Faculty Web Sites at the University of Virginia
BAC White Paper - Faculty Web Sites at the University of Virginia

... (~100,000 clones of 100kb) that could be useful for promoter mapping studies when appropriate distribution arrangements can be made. However in this case as well, no evaluation of the library has been made by users in the community. The advice we have received from numerous sources is that it will b ...
Chapter 11: Complex Inheritance and Human Heredity • Main idea
Chapter 11: Complex Inheritance and Human Heredity • Main idea

... • A recessive trait is expressed when the individual is homozygous recessive for the trait. • Therefore, those with at least one dominant allele will not express the recessive trait. • An individual who is heterozygous for a recessive disorder is called a carrier ...
Postdoctoral Research Associate
Postdoctoral Research Associate

...  Whole-exome sequencing (or custom SNV array) ...
Protein Building Blocks (PBBs): Toys for teaching the principles of
Protein Building Blocks (PBBs): Toys for teaching the principles of

... as follows: positive blocks have magnets embedded on four sides with all N sides facing out, negative blocks have magnets with all S sides facing out. Partially assembled blocks are shown in Figure 1. Thus, like charged particles repel each other, but unlike charges are attractive. Hydrophobic inter ...
No Slide Title
No Slide Title

... (Figure adapted with permission from “Developmental Genetics and Ontogenetic Psychology: Overdue Détente and Propositions from a Matchmaker” by I. Gottesman, 1974. In A.D. Pick (Ed.), Minnesota Symposium on Child Psychology, vol. 8, p 60. Copyright © 1974 by the University of Minnesota Press.) ...
d. Method Delivery: Lectures, assignments, tutorials and practicals
d. Method Delivery: Lectures, assignments, tutorials and practicals

... The course introduces students to the basic principles of classical genetics and inheritance as espoused by Mendel. It helps to build the foundations that will enable students to understand and apply genetic principles in advanced undergraduate and postgraduate studies. The course content logically ...
Introduction to Mendelian Genetics
Introduction to Mendelian Genetics

... • Genetics: – The study of heredity ...
REAL-TIME PCR
REAL-TIME PCR

... dye on the 5’ end and a non-fluorescent quencher dye on the 3’ end. The most commonly used hydrolysis probes are TaqMan® probes (Holland et al., 1991; Heid, 1996). Molecular beacons probes (Tyagi and Kramer, 1996; Tyagi et al., 1998) and Scorpions primers (Whitcombe et al., 1999; Thelwell et al., 20 ...
Identification and characterization of putative conserved IAM
Identification and characterization of putative conserved IAM

... sequences is computed using k-mer counting. A tree is then constructed from the triangular distance matrix computed from the pairwise similarities using UPGMA and a root is identified. The resulting tree is improved by building a new progressive alignment and a new tree is constructed by computing a ...
Modulation of CTCF Insulator Function by
Modulation of CTCF Insulator Function by

... CTCF plays diverse roles in the regulation of eukaryotic genes. A new study by Lefevre et al. in a recent issue of Molecular Cell reveals a novel mechanism in which noncoding RNA transcription and nucleosome repositioning evicts CTCF from a regulatory element to facilitate induction of a nearby gene ...
Cloning an iron-regulated metal transporter from rice
Cloning an iron-regulated metal transporter from rice

... ferrous iron (Yoshida, 1981). Therefore, it seems reasonable that rice may have an iron uptake system for Fe(II) as well as Fe(III) in its roots. Rice also transports oxygen from the shoots to the roots, and secretes oxygen from roots to oxidize the rhizosphere. Therefore, rice plants may induce one ...
DNA
DNA

...  ___DNA___ is a _polymer_ made of _repeating_ subunits called _nucleotides_ (the monomer).  _Nucleotides_ have three parts: a simple __sugar__, a _phosphate_ __group__, and a _nitrogenous_ ___base___.  In DNA there are ___four___ possible _nucleotides_ , each containing one of these four bases.  ...
Educational Item Section Architecture of the chromatin in the interphase Nucleus
Educational Item Section Architecture of the chromatin in the interphase Nucleus

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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