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Opposite deletions/duplications of the X chromosome: two
Opposite deletions/duplications of the X chromosome: two

... factor VIII, of the IDS gene and of the FLN1/emerin region, all resulting from misalignment of inverted repeats, and double recombination. Most of these inversions are of paternal origin possibly because the X chromosome at male meiosis is free to refold into itself for most of its length. We report ...
Topic 1: Statistical analysis (2 hours)
Topic 1: Statistical analysis (2 hours)

... from the rough endoplasmic reticulum to the Golgi apparatus; from the Golgi apparatus to the plasma membrane; formation of vesicle from plasma membrane allows material to be taken in; endocytosis / pinocytosis / phagocytosis / phagolysosome is absorption of material using a vesicle; fusion of vesicl ...
Acyl-ACP thioesterases from Camelina sativa: Cloning
Acyl-ACP thioesterases from Camelina sativa: Cloning

Tth RecA
Tth RecA

... Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here. ...
CCEB
CCEB

... Impact of Genetic Variability  Loss of gene = loss of function  Duplication of DNA segments and single base pair changes may have different effects depending on position  Gain of function, loss of function, no change ...
The Genetics of Beta-galactosidase
The Genetics of Beta-galactosidase

... wishes to test if the expression of a certain gene is controlled by environmental factors, a gene fusion with a promoter-less β-galactosidase can be used. This is especially useful if the gene product is uncharacterized or is not easily assayed. Along the same lines, the rate of gene expression can ...
Amino Acid Metabolism (day-2)
Amino Acid Metabolism (day-2)

... Tyrosine is also the precursor to pigment molecules called melanins that are produced from dopaquinone ...
Nikolayev Ì., Alekseyeva N.
Nikolayev Ì., Alekseyeva N.

... 1. Initial data for constructing a model When carrying out research the process of inbreeding (crossing of two closely related individuals) was studied. The scope of the given process is selection as it can help unite certain hereditary attributes in one organism and get rid of undesirable propertie ...
Cowden Syndrome
Cowden Syndrome

... What if testing does not detect an altered PTEN gene? Negative results (meaning a PTEN mutation is not found) can mean several things. First, it might mean that there is a PTEN gene mutation that cannot be located by current testing methods. Twenty percent of people with a diagnosis of Cowden syndr ...
Introduction to Genetics
Introduction to Genetics

...  He transferred male pollen to the female ovule (pollination)  Performed a controlled experiment by studying one trait at a time  Hybrid: offspring produced by parents with different traits Ex. Tall x Short  Cross pure tall plants with pure short plants = Hybrid offspring  What do the hybrids l ...
Construction of PANM Database (Protostome DB) for rapid
Construction of PANM Database (Protostome DB) for rapid

... However, it is found that the server has limitations in the annotation of molluscan sequences generated using next-generation sequencing (NGS) platforms due to inconsistencies in molluscan sequence available at NCBI. We constructed a web-based interface for a new stand-alone BLAST, called PANM-DB (P ...
Rules, regulations, and policies for breeding and biotechnology
Rules, regulations, and policies for breeding and biotechnology

... that many traits depend on many genes, so called quantitative traits, was understood and statistical models were developed to account for such traits in livestock breeding. As with evolution, breeding is dependent on genetic variation and the recombination of genes. However, genetic variation can be ...
Pol Is a Candidate for the Mouse Pulmonary
Pol Is a Candidate for the Mouse Pulmonary

... 30 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 1 min, and finally 72°C 0.01% xylene cyanol), heated to 94°C for 3 min, and separated by 12% for 6 min. Annealing temperature was optimized for each primer set. Electro- denaturing PAGE. Gels were quantified by phosphorimage analysis. phoreses o ...
Yvonne Gicheru Presentation
Yvonne Gicheru Presentation

... DNA binding properties using bandshift analysis Transactivation of luciferase reporter gene under Pax2 target sequence using CMV constructs transfected into NIH3 fibroblast cells • Measuring transcription of downstream genes - No sig difference between WT and mutant in transcript levels of target ge ...
Clone
Clone

... This antibody is intended for use to qualitatively identify MCM5 antigen by light microscopy in formalin fixed, paraffin embedded tissue sections using immunohistochemical detection methodology. Interpretation of any positive or negative staining must be complemented with the evaluation of proper co ...
A Genetical Genomics Project
A Genetical Genomics Project

... • General-purpose function for testing GO associations – genes is a vector of the transcript names under consideration – classification is a boolean vector the same length as genes, indicating which transcripts are in the classification – go is a matrix where go[transcript,Goterm] = TRUE if Goterm i ...
Detectie van quarantaine plagen op bedrijven BO-06-005 - q
Detectie van quarantaine plagen op bedrijven BO-06-005 - q

... Monochamus beetles) and isolated nematodes (e.g. trapped PWN). will be tested).  Select and validate three on-site DNA extraction procedures to optimize assay sensitivity. Modification of three proven DNA extraction methods towards simplest possible manipulation for direct-in field application (e.g ...
MitoP2, an integrated database on mitochondrial proteins in yeast
MitoP2, an integrated database on mitochondrial proteins in yeast

... listed separately. The reference set of 477 mitochondrial proteins has been sorted into major functional entities such as `protein translation/stability' or `biogenesis of iron±sulfur cluster'. The annotation of these categories is accomplished by a consortium of mitochondria researchers organized i ...
Bacteria & Archaea Prokaryote
Bacteria & Archaea Prokaryote

... • 2) Cocci/Coccus = Spherical • 3 Basic Shapes: • 3) Spirilla/Spirillum = Spiral ...
The effects of polymorphisms in DGAT1, GH and GHR genes
The effects of polymorphisms in DGAT1, GH and GHR genes

... 7. GE W., DAVIS M.E., HINES H.C., IRVIN K.M., 1999 – Two-allelic DGGE polymorphism detected in the promoter region of the bovine GHR gene. Animal Genetics 30, 71. 8. GRISART B., COPPIETERS W., FARNIR F., KARIM L., FORD C., BERZI P., CAMBISANO N., ...
WebGestaltR
WebGestaltR

... and do not need to upload the reference set. All existing platform supported in the WebGestaltR can be found by the function listReferenceSet.If referenceGeneFile and refereneceGene are emphNULL, WebGestaltR will use the referenceSet as the reference gene set. Otherwise, WebGestaltR will use the us ...
Consequences in the use of Genetically Modified
Consequences in the use of Genetically Modified

... No obvious hazards could be find in literature! ...
Microarray Construction: A personal perspective
Microarray Construction: A personal perspective

... ENSEMBL & BLASTN hits EST hits bovine human other Represent unique clusters ESTscan hits Singletons ...
S. marcescens - York College of Pennsylvania
S. marcescens - York College of Pennsylvania

... are among the most opportunistic pathogens and frequently encountered gram-negative organisms in nosocomial infections. Recent work has shown that gram-negative bacteria release membrane vesicles (MV), which contain proteins, lipopolysaccharides, phospholipids, RNA and DNA, from their surfaces durin ...
Guidance on the Use of Biochemical and Molecular Markers
Guidance on the Use of Biochemical and Molecular Markers

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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