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... Central dogma http://fig.cox.miami.edu/~cmallery/255/255hist/mcb4.1.dogma.jpg ...
Serology
Serology

... discovered that portions of DNA structure of certain genes are unique to each individual. Termed the process used to isolate & read these genetic markers as “DNA fingerprinting”. ...
Alternative Splicing Analysis Tools Through the UCSC Genome
Alternative Splicing Analysis Tools Through the UCSC Genome

... 5. Click on “Block 5” (representing exon 5). Exon 5 now appears on the upper part of the screen, in the right pane, in blue capital letters. 6. Select and copy the entire sequence of exon 5, along with 14 nucleotides in the upstream intron and 6 nucleotides in the downstream intron (you’ll also be c ...
What are chromosomes?
What are chromosomes?

... the intervening segment is rejoined in an inverted or opposite manner. Since there is no loss nor gain of chromosomal material, inversion carriers are normal Paracentric: does not include the centromere pericentric:inverted segment contains the centromere In meiosis, the normal chromosome and the in ...
Floral organ identity genes in the orchid
Floral organ identity genes in the orchid

... and one AP2-like gene were isolated from dormant flower buds or newly open flowers using PCR amplification of the cDNAs with degenerate primers as described in Experimental procedures. The rapid amplification of cDNA ends (RACE) method was employed to further isolate full-length cDNAs of AP2, AP3, P ...
The molecular biology of sex determination and sexual development
The molecular biology of sex determination and sexual development

... Accurate sexing of chickens can be carried out by PCR genotyping to amplify DNA sequences on the Wchromosome. The methods are particularly suitable for laboratory research involving embryos that cannot be sexed by morphology but is too time-consuming for routine sexing. In the poultry industry, rout ...
Identification of Full and Partial Class Relevant Genes
Identification of Full and Partial Class Relevant Genes

Full contents - Scion Publishing
Full contents - Scion Publishing

... The information contained within this book was obtained by Scion Publishing Ltd from sources believed by us to be reliable. However, while every effort has been made to ensure its accuracy, no responsibility for loss or injury whatsoever occasioned to any person acting or refraining from action as a ...
Document
Document

... upheaval in biomedical research. It makes it possible to correct errors in the genome and turn on or off genes in cells and organisms quickly, cheaply and with relative ease. It has a number of laboratory applications including rapid generation of cellular and animal models, functional genomic scree ...
Conserved expression of a root-hair specific promoter LeExt1.1 from
Conserved expression of a root-hair specific promoter LeExt1.1 from

... Using root hair specific promoter to target gene expression in trichoblasts has great potential to improve nutrient uptake and transformation from soil in cassava (Manihot esculenta Crantz), a crop usually growing on acidic soils low in nutrients, particularly phosphorus. For example, when expressin ...
Lecture 24 – PDF
Lecture 24 – PDF

... 2. Transfer RNAs: small molecules that are 4S or 70-90 nucleotides long. a) There are from 1-4 tRNA genes for each of the common 20 amino acid molecules. b) Amino amino acids are “loaded” onto tRNAs by amino acid-activating enzymes called amino-acyl tRNA synthetases. There is at least one amino-acid ...
Translation
Translation

... by peptide bond to the amino acid carried by the tRNA at the A site → at the same time tRNA carrying prolonged polypeptide chain is shifted from the A site to the P site and now free tRNA is shifted from the P site to E site → thus, the A site is freed for binding of further aminoacyl-tRNA → in the ...
JOIN2004 Universidade do Minho
JOIN2004 Universidade do Minho

Chloroplast Targeting, Distribution and Transcriptional Fluctuation of
Chloroplast Targeting, Distribution and Transcriptional Fluctuation of

... In Fig. 3, Southern hybridization analysis was performed under low stringency by the use of 5¢-half and 3¢-half probes, respectively. Both probes were hybridized with a single band in all lanes. The sizes of hybridized bands were well consistent with those estimated from the disclosing sequence data ...
Molecular Mechanisms of Long Noncoding RNAs
Molecular Mechanisms of Long Noncoding RNAs

Metabolism III
Metabolism III

... Lipids ...
Chapter 23 Slides
Chapter 23 Slides

... Example of Cline Based On Temperature Change with Climate ...
Chapter 15
Chapter 15

... The Chromosomal Basis of Sex • In humans and other mammals, there are two varieties of sex chromosomes: a larger X chromosome and a smaller Y chromosome • Only the ends of the Y chromosome have regions that are homologous with corresponding regions of the X chromosome • The SRY gene on the Y chromo ...
Technical document The purpose of this document is to help
Technical document The purpose of this document is to help

... as a basic training manual to enable you to interpret and use the resources and tools involved the annotation of the B. rapa BACs. It will also cover using other resources found on this website such as the Brassica 95K microarray database and the local blast server. These issues will be addressed by ...
SepF, a novel FtsZ-interacting protein required for a late step in cell
SepF, a novel FtsZ-interacting protein required for a late step in cell

Molecular Genetic Analysis of Tunisian Patients with a Classic Form
Molecular Genetic Analysis of Tunisian Patients with a Classic Form

... show a high homology with a nucleotide identity of 98% in their exon and 96% in their intron sequences (7, 8). The proximity and the high degree of homology between the two genes are believed to be the main reason for unequal crossover and gene conversion-like events, which give rise to mutations in ...
Flora of Jammu and Kashmir state (Family Asteraceae
Flora of Jammu and Kashmir state (Family Asteraceae

... such cultures often produces false positives due to cross feed of growth hormones to the co-cultivated tissue by the bacterial strain that facilitates growth and plant regeneration from the tissue in the hormone free medium. Further, during DNA isolation from regenerants of this types of culture, DN ...
UCSC Known Genes (by Jim Kent)
UCSC Known Genes (by Jim Kent)

... • Thousands of transcripts for these in Genbank. • Gaps are from genomic rearrangements, not splicing. Millions of possibilities. • Identify regions by: – Searching for words like ‘immunoglobulin’ ‘variable’ to make initial set of Ab fragments. – Treat anything that overlaps these as Ab fragment too ...
Looking through a Father`s Eyes
Looking through a Father`s Eyes

... series of building blocks. In the case of DNA however, there are only four building blocks called nucleotides: adenine, thymine, cytosine and guanine. We will refer to them as A, T, C and G. These are the letters in which the genetic code is written. These letters join together to make extremely lon ...
Translation
Translation

... by peptide bond to the amino acid carried by the tRNA at the A site → at the same time tRNA carrying prolonged polypeptide chain is shifted from the A site to the P site and now free tRNA is shifted from the P site to E site → thus, the A site is freed for binding of further aminoacyl-tRNA → in the ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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