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computation for chIP-seq and rNA-seq studies
computation for chIP-seq and rNA-seq studies

We`re on the brink of a DNA revolution – where your genetic code
We`re on the brink of a DNA revolution – where your genetic code

cis-Regulatory Elements and Chromatin State
cis-Regulatory Elements and Chromatin State

... This article is a Plant Cell Advance Online Publication. The date of its first appearance online is the official date of publication. The article has been edited and the authors have corrected proofs, but minor changes could be made before the final version is published. Posting this version online ...
Orientation of the transcription factor binding
Orientation of the transcription factor binding

... challenge is to expand the known repertoire of TF-target pairs by identifying novel Transcription Factor Binding Sites (TFBS) based on sequence data. One main difficulty in such computational predictions is the large number of false positives they generate. Here we examine the association of five fe ...
Origin and evolution of Y chromosomes: Drosophila tales
Origin and evolution of Y chromosomes: Drosophila tales

... genomes provides an opportunity to study the origin and evolution of Y chromosomes in an informative phylogenetic context. Surprisingly, the majority of Drosophila Y-linked genes are recent acquisitions from autosomes and Y chromosome gene gains are more frequent than gene losses. Moreover, the Dros ...
Dermatosparaxis type fact sheet
Dermatosparaxis type fact sheet

... disorder. Mutations of certain genes cause abnormal connective tissue synthesis, resulting in abnormal fragile, hyper-extensible tissue. Main symptoms include; easy bruising, atrophic (depressed) scarring and delayed wound healing. The syndrome is named after Danish and French dermatologists, Edvard ...
LocalStructureBystro..
LocalStructureBystro..

... For each cluster with good boundaries Clustering increases P(cluster|sequence) In order to increase P(structure|cluster) 2 residues are also observed on each side of each sequence • All segments that are not within the natural boundaries of the paradigm are removed • The frequency profile of the clu ...
Chapter 11 Notes - Plain Local Schools
Chapter 11 Notes - Plain Local Schools

... 16. What are some examples of multiple allele traits? (Think about traits that have more that two ...
E1. Mexican hairless dogs are heterozygous for a dominant allele
E1. Mexican hairless dogs are heterozygous for a dominant allele

... aaBB. Their offspring are AaBb and therefore are not albinos because they have one dominant copy of each gene. E6. It is a sex-limited trait where W (white) is dominant but expressed only in females. In this cross of two yellow butterflies, the male is Ww but is still yellow because the white phenot ...
Ch. 11 Intro to Genetics
Ch. 11 Intro to Genetics

... In the Punnett square shown in Figure 11-1, which of the following is true about the offspring resulting from the cross? ...
Document
Document

... the letters A and B represent the two different pigmentation genes, then one of the dogs is AAbb and the other is aaBB. Their offspring are AaBb and therefore are not albinos because they have one dominant copy of each gene. E6. It is a sex-limited trait where W (white) is dominant but expressed onl ...
Review #3 - California Lutheran University
Review #3 - California Lutheran University

... What is the structure of tRNA? What is its tertiary structure? What are aminoacyl tRNA synthetases? What are the two classes of synthetases and how do they differ? If there are 61 codons and 32 or more tRNAs, why are there only 20 synthetases? What parts of the tRNA do the synthetases recognize? Why ...
Human cloning - 10EssentialScience
Human cloning - 10EssentialScience

... the biological sense, therefore, is a single cell (like bacteria, lymphocytes etc.) or multi-cellular organism that has been directly copied from and is therefore genetically identical to another living organism. Sometimes this term can refer to "natural" clones made either when an organism reproduc ...
Molecular differentiation of species of the genus Zungaro - Funpec-RP
Molecular differentiation of species of the genus Zungaro - Funpec-RP

... Additionally, there are taxonomic inconsistencies in the Zungaro genus in Brazil that can affect conservation of natural populations of this fish. A previous study has described two morphologically distinct species in major Brazilian rivers (Lundberg and Littmann, 2003): the species Zungaro zungaro ...
Control of the acetamidase gene of Mycobacterium smegmatis by
Control of the acetamidase gene of Mycobacterium smegmatis by

Non-Enzymatic, Low Temperature Fluorescence in situ
Non-Enzymatic, Low Temperature Fluorescence in situ

... couples), alternative mechanisms for ISH may exist. For example, small amounts of single-stranded target DNA present either naturally, or as the result of the fixation process alone, might in some cases be sufficient to allow visualization of probetarget binding sites using low temperature protocols ...
Comparison of environmental and isolate Sulfobacillus genomes
Comparison of environmental and isolate Sulfobacillus genomes

... regions were extracted and trimmed with Sickle (available https://github.com/najoshi/sickle), allowing only paired-end reads with length >60 and quality scores >20. For the reference database, 186 sequences were downloaded from the SILVA SSU database representing the 174 sequences of ‘Family XVII In ...
“Have Transgenes, Will Travel”
“Have Transgenes, Will Travel”

... the probability of gene flow from crops to wild relatives. Norman Ellstrand, an expert on plant population genetics at the University of CaliforniaRiverside, has done extensive research on the capacity of cultivated crops to distribute their genetic material by breeding with wild relatives. He said ...
seq.
seq.

... fits into this category: inteins, selfsplicing introns, transposable elements, ion pumps, other transporters, Synologs: genes ended up in one organism through fusion of lineages. The paradigm are genes that were transferred into the eukaryotic cell together with the endosymbionts that evolved into m ...
Evaluation of genomic DNA from paraffin
Evaluation of genomic DNA from paraffin

In-Lab Handout
In-Lab Handout

... This is part of the work that the Perl program is doing for you. After you play with regex, we can look at the code if you are interested. Going back for more: Select regex_yourname.pl in the Documents Drawer to return to your Perl script. Edit your new regex in pink text between the single quotes, ...
PowerPoint Presentation - MCB 372
PowerPoint Presentation - MCB 372

... PAML (and other programs) allow to either determine omega for each site over the whole tree, ...
F 1 - OpenWetWare
F 1 - OpenWetWare

... locus is involved.] How many alleles do you think are involved? Which (if any) are dominant? 2. What breeding experiments could you do to test your hypothesis? (For example, what crosses could you do, or what data would you request from the breeder?) What results would falsify your hypothesis, and w ...
マニュアル Megaprime DNA Labelling System
マニュアル Megaprime DNA Labelling System

function finders
function finders

... -- Luciferase uses chemical energy to speed up the reaction between luciferin and oxygen to generate light. -- The adult firefly beetle uses bioluminescence to attract a mate. Other organisms also use bioluminescence including deep sea fish such as the Anglerfish which uses it to attract prey rather ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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