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Common types of DNA damage Different types of repair fix different
Common types of DNA damage Different types of repair fix different

... DNA repair by the baseexcision repair pathway (BER). (a) A DNA glycosylase recognizes a damaged base and cleaves between the base and deoxyribose in the backbone. (b) An AP endonuclease cleaves the phosphodiester backbone near the AP site. (c) DNA polymerase I initiates repair synthesis from the fre ...
RNA polymerase
RNA polymerase

... Eukaryotic cells modify mRNA after transcription. Splicing of mRNA increases the number of different proteins an organism can produce. Gene expression is regulated by proteins that bind to specific base sequences in DNA. The environment of a cell and of an organism has an impact on gene expression. ...
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GmDREB5 Tiendai, Vietnam Chu Hoang Lan Nguyen Vu Thanh Thanh
GmDREB5 Tiendai, Vietnam Chu Hoang Lan Nguyen Vu Thanh Thanh

... not only have high economic and nutritional value but only play an important role in improving soil fertility and sustainable use of cultivated land resources. In recent years, droughts have occurred more and more commonly as a result of global warming and climate change. This has affected the growt ...
BIOLOGICAL MOLECULES
BIOLOGICAL MOLECULES

... In this diagram a ..................................... enzyme cuts the DNA and another enzyme, known as ..................................... joins pieces back together. Other than plasmids, ........................... can also be used to transfer the gene. The bacterium with the gene from another ...
Using DNA to ID Pathogens
Using DNA to ID Pathogens

... this project, completed in 2003, scientists have determined the complete nucleotide sequence of the DNA of each human chromosome. In addition to mapping human DNA, the Human Genome Project has helped map the genomes of other species, including mice, the fruit fly, and various bacteria. Knowing the n ...
DNA and Evolution
DNA and Evolution

... sequence homology searches are conducted with protein sequences. – Three-dimensional shape, the key to enzyme function, is conserved better than protein sequence. It is quite possible to produce the same structure with completely different amino acids. • Unfortunately, it is very difficult to search ...
Identification of the chlB Gene and the Gene Product Essential for
Identification of the chlB Gene and the Gene Product Essential for

... a light-independent manner. However, very little information was available about such light-independent systems because suitable organisms for investigations by molecular genetic techniques have not been available. Cyanobacteria are prokaryotes with a photosynthetic apparatus similar to that of high ...
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Interaction of a Nuclear Protein with 5` Flanking Region of

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Chapter 6 Genes and Gene Technology Section 1 We now know

... __________________ are made up of amino acids linked together (we have had this before). The ____________ of the bases determines the order of the amino acids in a protein. Scientists thought or DNA was found in proteins at one time because proteins are so ________________. The first step in making ...
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Nucleic acid engineering

... Nucleic acids “old basic” functions 1) Contain information about protein structure (DNA) 2) Participate in selection and ligation of amino acids needed for proteins (RNA) ...
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PC Pc pC pc PC PPCC (purple) PPCc (purple) PpCC

... II-6: No, since you don’t know the parents’ genotypes (or phenotypes) so you won’t know the second allele until after they have a child. Once the child (III-4) is born homozygous recessive, then you know that II-6 must be a carrier for Tay-Sachs disease. 3) The mRNA for a newly discovered gene has a ...
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DISRUPTING GENETIC EQUILIBRIUM

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Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)

... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
Introduction to Molecular Biology
Introduction to Molecular Biology

... for protein production. Physically, DNA consists of two strands held together by hydrogen bonds, arranged in a double helix as shown in Fig. 2.3. The complement of a DNA sequence consists of complements of its bases. The DNA therefore consists of two complementary strands which bind to each other ti ...
Some Biology that Computer Scientists Need for
Some Biology that Computer Scientists Need for

... • Only certain genes are “turned on” at any particular time. • When a gene is transcribed (copied to mRNA), it is said to be expressed. • The mRNA in a cell can be isolated. Its contents give a snapshot of the genes currently being expressed. • Correlating gene expressions with conditions gives hint ...
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Reaxys Database Information Author keywords

... Iranian subpopulations of Holstein cattle (Moghan farm n = 250 and Razavi farm n = 175) was investigated by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Bovine DNA was isolated from whole blood. A hemi-nested PCR followed by digestion with restriction endonuc ...
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Lecture 12 - School of Science and Technology
Lecture 12 - School of Science and Technology

... • A coding statistic was introduced to measure uneven usage of synonymous codons solely. • Indeed, from a codon usage table, we can compute the relative probability of each synonymous codon to code for a given amino acid. • For instance, GAG and GAA the two codons coding for Glutamic Acid are used i ...
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chapter 19 the organization and control of eukaryotic

...  First, the typical multicellular eukaryotic genome is much larger than that of a prokaryotic cell.  Second, cell specialization limits the expression of many genes to specific cells.  The estimated 25,000 genes in the human genome include an enormous amount of DNA that does not code for RNA or p ...
Human DNA Dance - University of Wisconsin Biotechnology Center
Human DNA Dance - University of Wisconsin Biotechnology Center

... However, the Human DNA Model is among the easiest ways to show the idea of "antiparallel" because the two lines of people face opposite directions as they shake hands, like two teams after a baseball game. Few models of DNA actually show the antiparallel nature of DNA, and of the models that do show ...
Phylogenomics of Cold Adaptation in Bacteria and Archaea
Phylogenomics of Cold Adaptation in Bacteria and Archaea

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... Ribosomal RNA: The component of the Ribosome made of RNA Ribosome: a complex found in cells, made up of several subunits, each composed of proteins and RNA. Ribosomes are the site of manufacture of proteins. Ribozyme: An enzyme made exclusively or predominantly of RNA RNA: ribonucleic acid. A single ...
HW Answers pg. 241,2..
HW Answers pg. 241,2..

... mRNA survives in the cytoplasm and is translated into a functioning protein. • The capping and tailing of the primary mRNA transcript ensures that when the transcript exits the nucleus, it is not degraded by nucleases and phosphatases found in the cytoplasm. Capping also plays a role in the initiati ...
Biology StaAr review
Biology StaAr review

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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