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Viral replication factories/site(s) inside live host: Replication forks
Viral replication factories/site(s) inside live host: Replication forks

Document
Document

... Regulation is accomplished by sequence-specific DNA binding proteins. Binding may be promoter proximal or at a distance. DNA footprinting and mobility shift assays are used to investigate the binding of regulatory proteins. In the E. coli lac operon, there are both repressors and activators, each of ...
Chapter 13 Genetic Engineering, TE
Chapter 13 Genetic Engineering, TE

... When you read about related concepts, a compare-and-contrast table can help you focus on their similarities and differences. Construct a table to compare and contrast transformation in bacteria, plants, and animals. Look in Appendix A for more information about compare-and-contrast tables. Do your w ...
3-agents-for-evolutionary
3-agents-for-evolutionary

... DNA affects how it looks, how it behaves, and its physiology—all aspects of its life. So a change in an organism’s DNA can cause changes in all aspects of its life. • CROSSING OVER during meiosis produces variety within a species but MUTATION is the ultimate source of NEW genetic varieties in a spec ...
powerpoint
powerpoint

... Microscopic examination - likely behavior - responsiveness to treatment. ...
AG-BAS-02.471-05.4p m-Using_Biotechnology_to_Improve_Life
AG-BAS-02.471-05.4p m-Using_Biotechnology_to_Improve_Life

... • The difference between animals within the same species is called variability. • Traits that always show up in the offspring if they are present are said to be dominant; traits that can be covered up by other traits are recessive. Dominant trait in cattle is polled cattle. Recessive is red cattle. ...
Genomic Maps and Linkage Analysis
Genomic Maps and Linkage Analysis

... Genes can be mapped relative to each other based on linkage Genes can also be mapped relative to known DNA positions (“DNA markers” or polymorphic sites) along chromosomes …and thus these DNA markers serve as landmarks to establish the physical locations of genes in the genome ...
CF29v2 - DNA testing in Cystic Fibrosis - Bio
CF29v2 - DNA testing in Cystic Fibrosis - Bio

... Analysis of mutations in the CFTR gene is now performed as part of disease diagnosis and carrier testing protocols. Further to standard clinical techniques (e.g. sweat test), detection of a mutation in both copies of an individual’s CFTR genes provides definitive confirmation of the disease state. W ...
BAC vectors (Bacterial Artificial Chromosome)
BAC vectors (Bacterial Artificial Chromosome)

... capable of replicating and forming plagues on E. coli host cells. To prepare infectious λ virions carrying recombinant DNA, the phage assembly process is carried out in vitro. In one method, E. coli cells are infected with a mutant λ defective in A proteins, one of the two proteins required for pack ...
Examples of Branching Markov Processes
Examples of Branching Markov Processes

... such random variables as the number of descendants in the nth generation, or the probability that the family name will become extinct. 4. Survival of Mutant Genes: Each individual gene has a chance to give birth to k offspring, k = 1, 2, …, which are genes of the same kind. However, each individual ...
bch2ibm: molecular biology end of semester 1 exam notes 2014
bch2ibm: molecular biology end of semester 1 exam notes 2014

... Qu.  What  is  the  Shine-­‐Delgarno  sequence?   -­‐ It’s  a  ribosomal  binding  site  in  mRNA,  generally  located  8  basepairs   upstream  of  AUG   -­‐ Exists  only  in  prokaryotes   -­‐ The  six-­‐base  consensus  sequence  is  AGGAG ...
Making Recombinant DNA
Making Recombinant DNA

Identification and Characterization of a Novel Human Testis
Identification and Characterization of a Novel Human Testis

DNA Sequence Analysis
DNA Sequence Analysis

... analysis to calculate the significance vs. chance of the ...
Challenging traditional approaches to
Challenging traditional approaches to

... transducer. 'r' represents the 'read' symbol and 'p' represents the 'printed‘ symbol. B. Transition rules of this transducer. For each transition rule there is a transition molecule. ...
- 10EssentialScience
- 10EssentialScience

... DNA is packed into chromosomes You have 46 chromosomes in each of your body cells. You inherited these from your parents: 23 from mum and 23 from dad Click on the button to see what your chromosomes ...
Solid Tumour Section Soft tissue chondroma with t(3;12)(q27;q15) in Oncology and Haematology
Solid Tumour Section Soft tissue chondroma with t(3;12)(q27;q15) in Oncology and Haematology

... 667 kb of genomic DNA in the centromere-to-telomere orientation. The corresponding transcript is approximately 7,3 kb. The translation initiation codon is located in exon 3 and the stop codon in exon 11. Protein The open reading frame encodes a 612 amino acid protein. The protein is composed of a pr ...
Unit 2 Exam Biochem, Cell Bio, Metabolism
Unit 2 Exam Biochem, Cell Bio, Metabolism

... fish. Explain why the nucleic acids of the fish do not change your appearance. In what ways is the structure of mRNA similar to DNA? How does mRNA differ from DNA? What is the function of mRNA? What is the function of tRNA? What is a codon? What is an anticodon? Explain the interrelationship of thes ...
Give an account of gene mutation under the following
Give an account of gene mutation under the following

... 6. Gene mutation is a change in the bases/base type/base sequence/base order (nucleotide can be taken to be equivalent to base) 7. Substitution: base/bases are replaced with another/others 8. Inversion: order of bases reversed/bases turned around 9. Substitution/inversion may change base order of co ...
Exam 2
Exam 2

encouraging diversity : mcroevolution via selection
encouraging diversity : mcroevolution via selection

... one set of chromosomes, ensures that this set is unique and different from that of the parent. Random processes such as the transposition of DNA regions (“jumping genes”) occur in all biological domains. Bacteria divide by binary fission and do not have the random assortment processes that occur in ...
Genetically Modified Organisms (GMOs)
Genetically Modified Organisms (GMOs)

... Replicated many times through PCR or bacterial plasmids. 2 ways of getting the gene into the cell… - A vector (bacterial plasmids or viruses) - Shotgun method (bio-ballistics) Done in the embryonic stem cells. 2 types of genetic engineering - Positive eugenics - Negative eugenics ...
Silencing Bad Genes - Harvard Health Publications
Silencing Bad Genes - Harvard Health Publications

... In each case, wayward genes are the culprit. The boy inherited a defective gene that makes a misshapen version of the hemoglobin protein inside his red blood cells, causing sickle cell anemia. The lawyer has been infected by a hepatitis virus that has commandeered her liver cells, instructing them t ...
Topic guide 7.2: Regulation of gene expression
Topic guide 7.2: Regulation of gene expression

... non-coding piece of RNA (ncRNA) containing many stop sequences, which then smothers the chromosome, inactivating nearly all of its genes. There are also short (about 21 bases long) ncRNAs that target mRNAs to prevent translation. This is called post-transcriptional gene silencing. These miRNAs (micr ...
DNA and Chromosomes
DNA and Chromosomes

...  In humans and animals, it is the first step of mitosis, which occurs when cells in your body grow (like your muscles).  It is also very important for meiosis, which is the first step of sexual reproduction. ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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