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Laboratory Detection and Gene Cassette Stability of the
Laboratory Detection and Gene Cassette Stability of the

... blaGES-2 coding region, distinguishes this ESBL from blaGES-1 and the blaIBC-type genes, making it an ideal target for developing a novel sequence-specific, peptide nucleic acid (PNA)-based, multiplex-PCR detection method. Utilizing two primer pairs in conjunction with a PNA probe, this novel method ...
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... be more common in stem cells because they could control which type of cell the stem cell will become. In recent years, scientists discovered they could reprogram specialized cells back into non-specialized stem cells, simply by turning on several manager genes. They call these reprogrammed cells ind ...
Supplementary Material for: A scaling normalization method for
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Monster Central Dogma - Lincoln Park High School
Monster Central Dogma - Lincoln Park High School

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Analyzing Microarray Gene Expression Data

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... aeration to 1o8jml., centrifuged and resuspended in an equal volume of medium containing 2 pgjml. [3H]TDR (9 ejm.mole). In pulse-labelling experiments, incorporation of label was stopped by diluting the bacteria either 50-fold into medium containing 20 fLgjml. TDR or 250-fold into cold 0,15 ~1-NaCI ...
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`Sumo` rats set researchers on hunt for obesity genes

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Two Functional a-Tubulin Genes of the Yeast Saccharomyces cerevisiae Encode Divergent Proteins.
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... Department of Biology' and Center for Cancer Research,2 Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 Received March 1986/Accepted 23 June 1986 ...
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Varicella-Zoster Virus Specifies a Thymidylate Synthetase
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... residue protein with a molecular weight of 34531 (Davison & Scott, 1986). Fig. l shows an alignment of the amino acid sequence of this protein with those of TSs from seven other organisms. Thus, Fig. 1 contains data from two eukaryotic viruses [VZV, Herpesvirus saimiri (HVS)], two eukaryotes (Homo s ...
Epigenomics Workshop - Institute for Systems Genomics
Epigenomics Workshop - Institute for Systems Genomics

... Claire Francastel is the Research Director at INSERM (National Research Institute for Medical Research) and PI at the Epigenetics and Cell Fate, University Paris Diderot campus. Her work has been focused on the regulation of gene expression during normal and pathological cellular differentiation. Sh ...
Distinctive distribution of AIM1 polymorphism among major human
Distinctive distribution of AIM1 polymorphism among major human

... pygmaeus) and a Japanese macaque (Macaca fuscata) were also used. For the identification of molecular alterations in the AIM1 coding region, primers were designed to amplify seven exons of AIM1 separately (Table 1). After polymerase chain reaction (PCR) amplification, we then determined the nucleoti ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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