Presentations in Biochemistry for MS 1
... Hereditary spherocytosis (choice D) is characterized by intermittent hemolysis, but, unlike pyruvate kinase deficiency and glucose-6-phosphate dehydrogenase deficiency, oxidant drugs are not a specific ...
... Hereditary spherocytosis (choice D) is characterized by intermittent hemolysis, but, unlike pyruvate kinase deficiency and glucose-6-phosphate dehydrogenase deficiency, oxidant drugs are not a specific ...
FORMATION OF AMMONIA
... The two nitrogen atoms of urea are derived from two different sources, one from ammonia and the other directly from the alpha amino group of aspartic acid. Steps of urea cycle are the following Step 1. Formation of Carbamoyl Phosphate One molecule of ammonia condenses with CO2 in the presence of two ...
... The two nitrogen atoms of urea are derived from two different sources, one from ammonia and the other directly from the alpha amino group of aspartic acid. Steps of urea cycle are the following Step 1. Formation of Carbamoyl Phosphate One molecule of ammonia condenses with CO2 in the presence of two ...
Transamination and Reductive Amination
... transaminases (see Section 2.4.3) or by amino acid dehydrogenases (EC 1.4.1.–) using NADH or NADPH as cofactor. The synthetic usefulness of the transaminase reaction is diminished by the position of the equilibrium (Keq is often close to 1), which means that either complex mixtures result that are o ...
... transaminases (see Section 2.4.3) or by amino acid dehydrogenases (EC 1.4.1.–) using NADH or NADPH as cofactor. The synthetic usefulness of the transaminase reaction is diminished by the position of the equilibrium (Keq is often close to 1), which means that either complex mixtures result that are o ...
Exam_2005 - The University of Sydney
... Which of the following enzymes is most likely to catalyse a ‘rate limiting step’ in a pathway High Vmax enzyme that catalyses irreversible conversion of SP with the prevailing [S] being about the same as the Km of the enzyme. High Vmax enzyme that catalyses reversible conversion of SP with the pre ...
... Which of the following enzymes is most likely to catalyse a ‘rate limiting step’ in a pathway High Vmax enzyme that catalyses irreversible conversion of SP with the prevailing [S] being about the same as the Km of the enzyme. High Vmax enzyme that catalyses reversible conversion of SP with the pre ...
Aerobic respiration - Wesleyan
... Anaerobic Fermentation Aerobic respiration and fermentation both begin with glycolysis, which converts one molecule of glucose into two molecules of pyruvate After glycolysis, the two pathways diverge Fermentation is completed in the cytoplasm, yielding 2 ATP per glucose ...
... Anaerobic Fermentation Aerobic respiration and fermentation both begin with glycolysis, which converts one molecule of glucose into two molecules of pyruvate After glycolysis, the two pathways diverge Fermentation is completed in the cytoplasm, yielding 2 ATP per glucose ...
Research Communications
... expression of transgenes in plants. The targeting of transgenes into the chloroplast by using a suitable strategy with an efficient expression cassette containing all the required elements has been the need of the day. Majority (90%) of the 3000 different proteins necessary for fully functional chlo ...
... expression of transgenes in plants. The targeting of transgenes into the chloroplast by using a suitable strategy with an efficient expression cassette containing all the required elements has been the need of the day. Majority (90%) of the 3000 different proteins necessary for fully functional chlo ...
Ka and Kb from pH and Conductivity Measurements
... (1.75 10-5) because (i) the temperature is ∼20 °C and not 25 °C, (ii) the temperature and pH sensors were not temperature calibrated, and (iii) the starting pH of the deionized water was acidic (pH 6.6-6.7). Acid ionization constants between 1.4 10-5 and 2.8 10-5 were considered reasonable and ...
... (1.75 10-5) because (i) the temperature is ∼20 °C and not 25 °C, (ii) the temperature and pH sensors were not temperature calibrated, and (iii) the starting pH of the deionized water was acidic (pH 6.6-6.7). Acid ionization constants between 1.4 10-5 and 2.8 10-5 were considered reasonable and ...
Specific Activities of Enzymes of the Serine Pathway of Carbon
... serine hydroxymethyltransferase, hydroxypyruvate reductase, malate dehydrogenase, phosphoenolpyruvate carboxylase, serineglyoxylate aminotransferase and ATP malate lyase (Hersh & Bellion, 1972). The ratio of specific activities (methylamine-grown cells/ succinate-grown cells) was above 40 in each ca ...
... serine hydroxymethyltransferase, hydroxypyruvate reductase, malate dehydrogenase, phosphoenolpyruvate carboxylase, serineglyoxylate aminotransferase and ATP malate lyase (Hersh & Bellion, 1972). The ratio of specific activities (methylamine-grown cells/ succinate-grown cells) was above 40 in each ca ...
Differential diagnosis of (inherited) amino acid metabolism or
... amino acids there are special active transport mechanisms. A number of amino acids are sharing the same special transport mechanism (e.g. proline, hydroxyproline and glycine, or cystine, ornithine, lysine and arginine). Other amino acids are in competition for their transport (e.g. the branched-chai ...
... amino acids there are special active transport mechanisms. A number of amino acids are sharing the same special transport mechanism (e.g. proline, hydroxyproline and glycine, or cystine, ornithine, lysine and arginine). Other amino acids are in competition for their transport (e.g. the branched-chai ...
Cellular respiration
... mitochondrial matrix, and gets oxidized to CO2 while at the same time reducing NAD to NADH. NADH can be used by the electron transport chain to create further ATP as part of oxidative phosphorylation. To fully oxidize the equivalent of one glucose molecule, two acetyl-CoA must be metabolized by the ...
... mitochondrial matrix, and gets oxidized to CO2 while at the same time reducing NAD to NADH. NADH can be used by the electron transport chain to create further ATP as part of oxidative phosphorylation. To fully oxidize the equivalent of one glucose molecule, two acetyl-CoA must be metabolized by the ...
Oxidative Phosphorylation - Creighton Chemistry Webserver
... Oxidative Phosphorylation What is it? Process in which ATP is formed as a result of the transfer of electrons from NADH or FADH2 to O2 via a series of electron carriers Oxidation of glucose: Glycolysis: 2ATP 2NADH (5ATP) Pyruvate dehyd: 2NADH (5ATP) Citric acid cycle: 2ATP 6NADH (15ATP) Oxidative ph ...
... Oxidative Phosphorylation What is it? Process in which ATP is formed as a result of the transfer of electrons from NADH or FADH2 to O2 via a series of electron carriers Oxidation of glucose: Glycolysis: 2ATP 2NADH (5ATP) Pyruvate dehyd: 2NADH (5ATP) Citric acid cycle: 2ATP 6NADH (15ATP) Oxidative ph ...
Hepatology: Anatomy, Physiology and Dev
... Bilirubin conjugation is an example Many other organic compounds undergo two-step biotransformation Example: cholesterol to bile acids After biotransformation, metabolites excreted Larger, lipophilic molecules excreted in bile Smaller (<400 Da) transported to blood and excreted by kidneys ...
... Bilirubin conjugation is an example Many other organic compounds undergo two-step biotransformation Example: cholesterol to bile acids After biotransformation, metabolites excreted Larger, lipophilic molecules excreted in bile Smaller (<400 Da) transported to blood and excreted by kidneys ...
Full-Text PDF
... 143B.TK- cells after switching the the culture medium from unlabeled to labeled amino acids at different time points. Shown are 13C15N culture medium from unlabeled to labeled amino acids at different time points. Shown are 13 C15 N amino acid ratios at (a) 2.5 min, (b) 5 min, (c) 10 min, and (d) 20 ...
... 143B.TK- cells after switching the the culture medium from unlabeled to labeled amino acids at different time points. Shown are 13C15N culture medium from unlabeled to labeled amino acids at different time points. Shown are 13 C15 N amino acid ratios at (a) 2.5 min, (b) 5 min, (c) 10 min, and (d) 20 ...
Purification and Partial Characterization of an Acid
... slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sephadex G-100. This suggested that the faster moving component on SDS-polyacrylamide gel el ...
... slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sephadex G-100. This suggested that the faster moving component on SDS-polyacrylamide gel el ...
Biology: Concepts and Connections, 6e
... 47) A scientist suspects that the food in an ecosystem may have been contaminated with radioactive nitrogen over a period of months. Which of the following substances could be examined for radioactivity to test the hypothesis? A) the cell walls of plants growing in the ecosystem B) the hair produced ...
... 47) A scientist suspects that the food in an ecosystem may have been contaminated with radioactive nitrogen over a period of months. Which of the following substances could be examined for radioactivity to test the hypothesis? A) the cell walls of plants growing in the ecosystem B) the hair produced ...
Purification and Partial Characterization of an Acid
... slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sephadex G-100. This suggested that the faster moving component on SDS-polyacrylamide gel el ...
... slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sephadex G-100. This suggested that the faster moving component on SDS-polyacrylamide gel el ...
Section 3. Antimicrobial Sulfonamides and Antibacterial Synergists
... Antimetabolites are compounds that prevent the biosynthesis or use of normal cellular metabolites. The usually are closely related in structure to the metabolite that is antagonized. Many antimetabolites are enzyme inhibitors. They may combine with active site as if they were the substrate or cofact ...
... Antimetabolites are compounds that prevent the biosynthesis or use of normal cellular metabolites. The usually are closely related in structure to the metabolite that is antagonized. Many antimetabolites are enzyme inhibitors. They may combine with active site as if they were the substrate or cofact ...
Cellular Respiration
... • A common fuel molecule for cellular respiration is glucose. • Cellular respiration can produce up to 32 ATP molecules for each glucose molecule consumed. ...
... • A common fuel molecule for cellular respiration is glucose. • Cellular respiration can produce up to 32 ATP molecules for each glucose molecule consumed. ...
Dynamic Modeling of Lactic Acid Fermentation Metabolism with
... biotechnology-produced PLA can become an alternate material of fossil fuel-based chemicals, the amount of CO2 emissions can be significantly reduced to step towards the goal of sustainable development. For these kinds of studies, systems biotechnology is an essential tool for the prediction and anal ...
... biotechnology-produced PLA can become an alternate material of fossil fuel-based chemicals, the amount of CO2 emissions can be significantly reduced to step towards the goal of sustainable development. For these kinds of studies, systems biotechnology is an essential tool for the prediction and anal ...
BCMB 3100 – Chapter 3 (part 1)
... Average M.W. for an amino acid is ____________ so M.W. of most proteins is 5500 to 220,000 daltons. (One dalton equals one atomic mass unit; kilodalton = 1000 daltons). Most proteins have M.W. of 5.5-220 kd. Some proteins contain disulfide bonds that cross-link between _________residues by the oxida ...
... Average M.W. for an amino acid is ____________ so M.W. of most proteins is 5500 to 220,000 daltons. (One dalton equals one atomic mass unit; kilodalton = 1000 daltons). Most proteins have M.W. of 5.5-220 kd. Some proteins contain disulfide bonds that cross-link between _________residues by the oxida ...
Acid – base balance
... All the known low molecular weight and water soluble biosynthetic intermediates possess groups that are almost completely ionised at neutral pH’ ...
... All the known low molecular weight and water soluble biosynthetic intermediates possess groups that are almost completely ionised at neutral pH’ ...
Partial Class Notes Chapters 3 and 5 (4 slides/page)
... one-sided, typed page, the amino acid mutated, the phenotype of the effect on the organism, the molecular reason that the mutation causes the effect(s), and the effect that this mutation has on/for humans. Hand in a single, one-sided, typed sheet of paper with ALL group members names (first and last ...
... one-sided, typed page, the amino acid mutated, the phenotype of the effect on the organism, the molecular reason that the mutation causes the effect(s), and the effect that this mutation has on/for humans. Hand in a single, one-sided, typed sheet of paper with ALL group members names (first and last ...
POLYPEPTIDE SEQUENCING
... is based around the study of the entire set of proteins produced by a cell (known as the proteome, c.f. genetics and genome). ...
... is based around the study of the entire set of proteins produced by a cell (known as the proteome, c.f. genetics and genome). ...
Biologically active octapeptides
... tion, the fragments can be easily dispersed into single groups to be employed in preparing the compounds of cells by repeated suction and expulsion from a Gilson this invention remains a matter well within one ordinar Pipetman. After 30 to 60 Pipetman operations, the tissue ily skilled in the art, i ...
... tion, the fragments can be easily dispersed into single groups to be employed in preparing the compounds of cells by repeated suction and expulsion from a Gilson this invention remains a matter well within one ordinar Pipetman. After 30 to 60 Pipetman operations, the tissue ily skilled in the art, i ...
Chemical Energy Production
... • Lactate moves from muscle to blood; pyruvate cannot leave muscle • Lactate moves from blood to liver – in liver, lactate is converted back to pyruvate – pyruvate is converted back to glucose – glucose can enter bloodstream and return to muscle for energy production or be stored in liver as glycoge ...
... • Lactate moves from muscle to blood; pyruvate cannot leave muscle • Lactate moves from blood to liver – in liver, lactate is converted back to pyruvate – pyruvate is converted back to glucose – glucose can enter bloodstream and return to muscle for energy production or be stored in liver as glycoge ...
Fatty acid synthesis
Fatty acid synthesis is the creation of fatty acids from acetyl-CoA and malonyl-CoA precursors through action of enzymes called fatty acid synthases. It is an important part of the lipogenesis process, which – together with glycolysis – functions to create fats from blood sugar in living organisms.