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NIH Public Access
NIH Public Access

... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
Nonphosphorylating Glyceraldehyde-3-Phosphate
Nonphosphorylating Glyceraldehyde-3-Phosphate

... Therefore, phosphorylation plus the interaction with 143-3 proteins determine a divalent cation-dependent regulatory mechanism to inhibit np-Ga3PDHase activity at high energy value (Bustos and Iglesias, 2003). This regulation is the rationale behind the cytosolic metabolism of plant cells where PPi ...
University: Suez Canal University Faculty of Medicine Course
University: Suez Canal University Faculty of Medicine Course

Supporting Information Legends Figure S1. Lipid and fatty acid
Supporting Information Legends Figure S1. Lipid and fatty acid

... phosphate. Data are means and SD of at least 3 measurements and were confirmed by a second independent experiment. Values significantly different to -P mock are indicated with an asterisk (Student's t test, p < 0.05). Figure S3. Fatty acid synthesis and degradation in R. irregularis. (a) Possible pa ...
The pivotal roles of mitochondria in cancer: Warburg and beyond
The pivotal roles of mitochondria in cancer: Warburg and beyond

... toward this metabolic transformation can be distilled to a few select enzymes; notably hexokinase, several mitochondrial associated enzymes and plasma membrane transporters, and the crucial organelle involved in energy generation, the mitochondrion. We will first examine the membrane transporters tha ...
BioAssay Systems Kinase
BioAssay Systems Kinase

... handling systems for tens of thousands of assays per day. ...
Missouri State Standards (Biology I End of Course Exam) Biology
Missouri State Standards (Biology I End of Course Exam) Biology

... Strand 3 Diversity and Unity Among Organisms -17 Major Conceptual Understandings I. There is a fundamental unity underlying the diversity of all living organisms 1. Recognize cells both increase in number and differentiate, becoming specialized in structure and function, during and after embryonic d ...
Identification, Expression and Characterization of Archaeal
Identification, Expression and Characterization of Archaeal

... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
CHAPTER 6
CHAPTER 6

... – Glucagon (29 AA-res) is also secreted from -cells in pancreas and acts in liver and adipose tissue only – Epinephrine (adrenaline) is released from adrenal glands and acts on liver and muscles ...
The monocarboxylate transporter family
The monocarboxylate transporter family

Crystal structure of mouse coronavirus receptor
Crystal structure of mouse coronavirus receptor

... fusion protein (17). During maturation, the spike protein is often cleaved into a receptor-binding subunit S1 and a membranefusion subunit S2 that associate together through noncovalent interactions (Fig. 1A). S1 sequences are relatively well conserved within each coronavirus group, but differ marke ...
Carotenoids Biosynthesis – a review
Carotenoids Biosynthesis – a review

... Carotenoids are naturally occurring pigments synthesized as hydrocarbons (carotenes) and their oxygenated derivatives (xanthophylls) by plants and microorganisms. They consist of eight isoprenoid units joined in such a manner that the arrangement of isoprenoid units is reversed at the center of the ...
Novel Substrates for Fluorescence-based Protein Tyrosine Kinase
Novel Substrates for Fluorescence-based Protein Tyrosine Kinase

... the microplates were sealed with TopSeal-A™ (PKI) during that time. The fluorescence signals were read using an excitation filter of 320 nm and an emission filter of 665 nm on an EnVision® Multilabel Reader (PKI). The final assay volume was 20 µL. Determination of kinase concentration – 0 to 30 nM o ...
05. Metabolism of carbohydrates 1
05. Metabolism of carbohydrates 1

... enzyme lactase, which cleaves lactose into glucose and galactose. Microorganisms in the colon ferment undigested lactose to lactic acid generating methane (CH4) and hydrogen gas (H2). The gas produced creates the uncomfortable feeling of gut distention and the annoying problem of flatulence. The lac ...
Biochemistry 304 2014 Student Edition Gluconeogenesis Lectures
Biochemistry 304 2014 Student Edition Gluconeogenesis Lectures

... •Understand how the gluconeogenesis pathway is regulated and how it is regulated vs ...
Protein Posttranslational Modifications: The Chemistry of Proteome
Protein Posttranslational Modifications: The Chemistry of Proteome

... in eukaryotic genomes to generate diversity in the corresponding proteomes, the inventory of all proteins in a cell or organism. Proteomes may be two to three orders of magnitude more complex (> 1 000 000 molecular species of proteins) than the encoding genomes would predict. The first route of dive ...
Hypolipidemic Drugs
Hypolipidemic Drugs

... • Increase the excretion of bile acids, causing more CHO to be converted to BAs. • The decr in hepatocyte [CHO]  compenatory incr in HMG CoA reductase activity and the number of LDLRs. • Because these resins don’t work in patients with homozygous familial hypercholesterolemia, increased expression ...
GSS: Gonad-Stimulating Substance
GSS: Gonad-Stimulating Substance

... MATURATION AND OVULATION IN THE STARFISH, ASTERINA PECTINIFERA Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to the vertebrate luteinizing hormone (LH). Here, we purified GSS o ...
Bacterial physiological adaptations to contrasting edaphic
Bacterial physiological adaptations to contrasting edaphic

... resolved is whether these differences in taxonomic diversity reflect changes in functional genetic potential. Many dominating organisms, particularly in the more oligotrophic habitats are difficult to culture, so we know little of the functional characteristics of these organisms at either the pheno ...
Catalogue Code: BA0125 Pack Size: 400 assays
Catalogue Code: BA0125 Pack Size: 400 assays

... KINASES, also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kin ...
Chapter 2 - University of the Free State
Chapter 2 - University of the Free State

Arginine-induced stimulation of protein synthesis and survival in
Arginine-induced stimulation of protein synthesis and survival in

... suggest that arginine deprivation decreases cell proliferation and heat shock protein expression and enhances the susceptibility to apoptosis, and most changes induced by arginine deficiency were restored by subsequent supplementation with arginine or citrulline (22). The essentiality of arginine fo ...
Uric acid
Uric acid

... Methotrexate inhibits dihydrofolate reductase. Cells cannot produce adequate amounts of dTMP for DNA synthesis. In this way methotrexate inhibits proliferation of cancer cells. ...
Activation of Peroxisome Proliferator-Activated Receptors
Activation of Peroxisome Proliferator-Activated Receptors

... common and persistent environmental enhanced by their metabolism catalyzed by CYP4A enzymes (10-14). Moreover, pollutant, and has been found in over one- liver cytochrome P450 (CYP) enzymes, chronic administration of DHEA can lead third of hazardous waste sites and in 10% of which produce multiple r ...
Problem-Set Solutions
Problem-Set Solutions

... phosphoenolpyruvate, can also act as an intermediate in the first step of the citric acid cycle; oxaloacetate combines with acetyl CoA, which can go directly into the citric acid cycle. 24.70 GTP and ATP 24.71 Lactate formed by muscle activity diffuses into the blood and is carried to the liver wher ...
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Biochemical cascade

A biochemical cascade (or a signaling pathway) is a series of chemical reactions which are initiated by a stimulus (first messenger) acting on a receptor that is transduced to the cell interior through second messengers (which amplify the initial signal) and ultimately to effector molecules, resulting in a cell response to the initial stimulus. At each step of the signaling cascade, various controlling factors are involved to regulate cellular actions, responding effectively to cues about their changing internal and external environments.
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