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Peroxyl free radicals: potential mediators of tumor initiation and
Peroxyl free radicals: potential mediators of tumor initiation and

... We attempted to investigate the source of peroxyl radicals in the epidermal cells. The high prostaglandin biosynthetic capacity of epidermal cells from hairless mice makes arachidonic acid metabolism a likely pathway. However, the prostaglandin H synthase inhibitor indomethacin has no effect on BP-7 ...
Transcriptome and metabolite profiling the infection cycle of
Transcriptome and metabolite profiling the infection cycle of

... immunity (PTI). Successful pathogens suppress PTI by secreting protein effectors which interfere with perception or defense signaling. However plants can also detect the function of these effectors using resistance proteins which monitor the function of effector targeted proteins or recognize the ef ...
Epoxyeicosatrienoic Acids Increase Intracellular
Epoxyeicosatrienoic Acids Increase Intracellular

... influx of extracellular Ca21.10 Likewise, 11,12-EET enhances the L-type Ca21 current in adult rat ventricular myocytes, although the Ca21 influx was not blocked by a voltage-dependent Ca21 channel antagonist.10,13 However, in a recent study EETs were reported to inhibit cardiac L-type Ca21 channel a ...
High-resolution analysis of metabolic cycles in the intertidal mussel
High-resolution analysis of metabolic cycles in the intertidal mussel

... Submitted 11 August 2011; accepted in final form 12 October 2011 ...
Metabolism
Metabolism

... • In the mouth, salivary amylase hydrolyzes aglycosidic bonds in polysaccharides to give smaller polysaccharides (dextrins), maltose, and some glucose. • In the small intestine, pancreatic amylase hydrolyzes dextrins to maltose and glucose. • The disaccharides maltose, lactose, and sucrose are hydro ...
Slide 1
Slide 1

... We must remember that both mitochondria and LDH enzymes co-exist in cells and therefor they “compete” for the pyruvate that is produced by glycolysis. Because the muscle form of LDH maintains [lactate] >> {pyruvate] some of the pyruvate produced through glycolysis will always be converted to lactate ...
1 Development of Bombesin Analogues With Conformationally
1 Development of Bombesin Analogues With Conformationally

... under nitrogen, and washed with 100 µl of 0.5 M potassium phosphate solution (pH 7.4). To this tube 20 µl of potassium phosphate solution (pH 7.4), 8 µg of peptide in 4 µl of water and 2 mCi (20 µl) of Na125I were added and incubated at room temperature for 6 min. The incubation was stopped by the a ...
IV.1 Structural and Functional Role of
IV.1 Structural and Functional Role of

... form the correct retinal binding pocket, it is intriguing as to why they are still deleterious. We constructed and expressed the synthetic G51L mutant to explore the effect of the size of the amino acid side chain in the packing of the helices and in signal transduction. The correctly folded fractio ...
Genomics Insights esTs from seeds to Assist the selective Breeding
Genomics Insights esTs from seeds to Assist the selective Breeding

... these, the Kennedy pathway, relies on the sequential acylation process of fatty acid biosynthesis of a glycerol-3-phosphate (Gly-3-P) backbone provided by GPDH. The second route relies on acyl-exchange between lipids and involves a phospholipid diacylglycerol acyltransferase (PDAT). Basically, TAGs ...
Identification of the Human Erythrocyte Glucose Transporter (GLUT1
Identification of the Human Erythrocyte Glucose Transporter (GLUT1

... cytosolic determinants of ...
CARBOHYDRATE METABOLISM
CARBOHYDRATE METABOLISM

... • The phosphatase reaction is favored by the phosphorylated form of the enzyme. This is NOT a reversal of the kinase reaction (don't re-create ATP). • The liver enzyme is phosphorylated by cAMP-dependent protein kinase. • cAMP levels rise in response to hormones such as glucagon and epinephrine and ...
Lipids as Tumoricidal Components of Human
Lipids as Tumoricidal Components of Human

... MAPK signaling, and especially Na⫹ or K⫹ fluxes were essential for these responses to occur. In a screen for suitable fatty acids cofactors, C18:1, cis-monounsaturated fatty acids were identified as optimal for HAMLET formation (26), suggesting that these fatty acids may share specific structural fe ...
BioCore II lecture20-S2015
BioCore II lecture20-S2015

... two blue carbons are converted to CO2 α-Ketoglutarate ...
Bioretrosynthetic Construction of a Non
Bioretrosynthetic Construction of a Non

... the past several years. In addition to Dr. Bachmann, I would also like to thank my Dissertation Committee members of Dr. Richard Armstrong, Dr. Tina Iverson, Dr. Lawrence Marnett and Dr. Carmello Rizzo for their valued discussions and for offering their time and energy in assisting my training as a ...
New approaches for live cell imaging
New approaches for live cell imaging

... Tubulin monomer MAP4 ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved

... For genes where the putative open reading frame could be determined based on sequence alignments, PCR primers were designed to amplify entire coding regions (Primer 3 in Geneious Pro v. 4.8.5; Rozen & Akaletsky 2000, Drummond et al. 2010) (Table 1). PCR reactions (25µl) using pooled cDNA containing ...
the role of prostanoids in the urinary bladder function and a potential
the role of prostanoids in the urinary bladder function and a potential

... They are synthetized from either ω-3 (eicosapentaenoic; EPA ñ with 5 double bonds) or ω-6 (arachidonic; AA ñ with 4 double bonds or dihomo-γlinolenic; DGLA ñ with 3 double bonds), 20-carbon, ...
Pathways to abscisic acid-regulated gene expression
Pathways to abscisic acid-regulated gene expression

... ‘ reverse genetics ’ (starting from unknown ABA-inducible sequences and working backwards) and biochemical studies. What emerges is a cell-biological model of overlapping tissue-specific stress (e.g. drought, salt and cold) and developmental (e.g. sugars and other hormones) response pathways that in ...
Sanguinarine (Pseudochelerythrine) Is a Potent Inhibitor of NF
Sanguinarine (Pseudochelerythrine) Is a Potent Inhibitor of NF

... cells with tumor necrosis factor rapidly activated NFkB, this activation was completely suppressed by sanguinarine in a dose- and time-dependent manner. Sanguinarine did not inhibit the binding of NF-kB protein to the DNA but rather inhibited the pathway leading to NF-kB activation. The reversal of ...
Regulation of 7-Dehydrocholesterol Reductase by Vitamin D3
Regulation of 7-Dehydrocholesterol Reductase by Vitamin D3

... Cholesterol is a key component of biological membranes. It is nonrandomly distributed between cellular organelles, subdomains of membranes, and leaflets of the membrane bilayer. In eukaryotic cells, about 65%-90% of cellular free cholesterol may reside in the plasma membrane where its average concen ...
Histidine Biosynthetic Pathway and Genes: Structure
Histidine Biosynthetic Pathway and Genes: Structure

... initiation and termination, RNA processing, and enzymology. In addition, the system has been extensively investigated not only in enterobacteria but also in many other species (grampositive and gram-negative bacteria, archaebacteria, and eukaryotic organisms), affording a unique opportunity to study ...
Alpha and beta subunits of pyruvate dehydrogenase E1
Alpha and beta subunits of pyruvate dehydrogenase E1

The relevance of carbon dioxide metabolism in
The relevance of carbon dioxide metabolism in

... DcarB. The DcarB gene was obtained by PCR, as previously described (Arioli et al., 2007; Mora et al., 2004). Briefly, DNA fragments located upstream and downstream of the 1687 bp deletion were independently amplified using CarB1–CarB2 and CarB3–CarB4 primer pairs (CarB1, 59-CTATTATCATTGGTCAGGCT-39; ...
NAD - wwphs
NAD - wwphs

... Enzymes (Cytochromes) and ATP synthase on membrane to shuttle electrons and protons NADH, FADH2 gives up the H H+ + e Electrons are passed through the membrane proteins and the energy released is used to transport the H to the outer membrane The gradient established is the force needed to allow the ...
Glucose
Glucose

... combining with oxaloacetate to form citrate containing three carboxyls. Two carbon atoms emerged from the cycle as CO2 from the oxidation of isocitrate and α-ketoglutarate. The energy released by these oxidations was conserved in the reduction of three NAD+ and one FAD and the production of one ATP ...
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Biochemical cascade

A biochemical cascade (or a signaling pathway) is a series of chemical reactions which are initiated by a stimulus (first messenger) acting on a receptor that is transduced to the cell interior through second messengers (which amplify the initial signal) and ultimately to effector molecules, resulting in a cell response to the initial stimulus. At each step of the signaling cascade, various controlling factors are involved to regulate cellular actions, responding effectively to cues about their changing internal and external environments.
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