Protein 1 File
... the SAME blue colored product. This reaction is performed post-column, after Ion Exchange Chromatography separation of a mixture of amino acids. The area of each peak in the chromatogram is proportional to the relative molar amount of the amino acid of that retention time. ...
... the SAME blue colored product. This reaction is performed post-column, after Ion Exchange Chromatography separation of a mixture of amino acids. The area of each peak in the chromatogram is proportional to the relative molar amount of the amino acid of that retention time. ...
YSK1 is activated by the Golgi matrix protein GM130 and plays a
... Phosphorylation at the equivalent position of the T-loop in many other kinases is an important determinant for their activation (Russo et al., 1996). Mutation of the T-loop threonine to alanine to give YSK1T174A resulted in a form of the kinase that showed no activity toward MBP in the presence or a ...
... Phosphorylation at the equivalent position of the T-loop in many other kinases is an important determinant for their activation (Russo et al., 1996). Mutation of the T-loop threonine to alanine to give YSK1T174A resulted in a form of the kinase that showed no activity toward MBP in the presence or a ...
Endoplasmic Reticulum Stress and Pancreatic Cancer
... Another pathway starts with the activation of IRE1α by homodimerization and autophosphorylation. After activation, IRE1 α interacts with TNF receptor-associated factor-2 (TRAF 2). TRAF2 activates apoptosis signaling kinase 1 (ASK1) and they form a complex. This complex subsequently activates the JNK ...
... Another pathway starts with the activation of IRE1α by homodimerization and autophosphorylation. After activation, IRE1 α interacts with TNF receptor-associated factor-2 (TRAF 2). TRAF2 activates apoptosis signaling kinase 1 (ASK1) and they form a complex. This complex subsequently activates the JNK ...
Full-Text PDF
... Lactation regulation has been the subject of much scientific and clinical interest for several decades. More specifically, the effect of hormones and amino acids on lactation in the dairy cow has been studied since the last century [1–3]. Leucine and other essential amino acids have been linked to p ...
... Lactation regulation has been the subject of much scientific and clinical interest for several decades. More specifically, the effect of hormones and amino acids on lactation in the dairy cow has been studied since the last century [1–3]. Leucine and other essential amino acids have been linked to p ...
Fibrous proteins
... Have a strong tendency to bind oxygen. Free iron can form of highly reactive oxygen species such as hydroxyl radicals that can damage DNA and other macromolecules. Therefore, iron used in cells is bound in forms that sequester it and/or make it less reactive. ...
... Have a strong tendency to bind oxygen. Free iron can form of highly reactive oxygen species such as hydroxyl radicals that can damage DNA and other macromolecules. Therefore, iron used in cells is bound in forms that sequester it and/or make it less reactive. ...
Angiotensin-Induced Desensitization of the Phosphoinositide
... apparent functional desensitization was not due to degradation of the peptide, since the cells were constantly superfused with Ang 11-containing buffer in these experiments. Previous results5,15 have supported the idea that the effects of Ang II are mediated by protein kinase C in cultured heart cel ...
... apparent functional desensitization was not due to degradation of the peptide, since the cells were constantly superfused with Ang 11-containing buffer in these experiments. Previous results5,15 have supported the idea that the effects of Ang II are mediated by protein kinase C in cultured heart cel ...
Induction of CDPK (ZmCPK11)- and MAPK
... increased susceptibility to certain fungal pathogens and insects (Browse 2009). It has been demonstrated that JA is responsible for the systemic response, functioning as a long-distance signal molecule transmitting the information about wounding to distant, non-wounded tissues where a defense respon ...
... increased susceptibility to certain fungal pathogens and insects (Browse 2009). It has been demonstrated that JA is responsible for the systemic response, functioning as a long-distance signal molecule transmitting the information about wounding to distant, non-wounded tissues where a defense respon ...
The integrin–actin connection, an eternal love affair
... cytoskeleton connection is highly dynamic and subject to many regulatory processes. In healing skin wounds for example, integrin-mediated cues promote the reorganization of the cytoskeleton of keratinocytes at the wound edge resulting in directed migration and wound closure. Loss of b1-integrins on ...
... cytoskeleton connection is highly dynamic and subject to many regulatory processes. In healing skin wounds for example, integrin-mediated cues promote the reorganization of the cytoskeleton of keratinocytes at the wound edge resulting in directed migration and wound closure. Loss of b1-integrins on ...
Whole-cell biocatalysts by design - Microbial Cell Factories
... produce a wide variety of products in various industries [3, 4]. The most important advantage of a biocatalyst is its high selectivity. The high selectivity, including regio-, chemo-, diastereo- and enantioselectivity, is very desirable in chemical synthesis and produces benefits such as ...
... produce a wide variety of products in various industries [3, 4]. The most important advantage of a biocatalyst is its high selectivity. The high selectivity, including regio-, chemo-, diastereo- and enantioselectivity, is very desirable in chemical synthesis and produces benefits such as ...
Protein Kinase C–dependent Activation of Cytosolic
... buffer for SDS-PAGE and Western blotting or in the assay buffer supplemented with 5 mM DTT for the cPLA2 activity assay. Phosphorylation-induced mobility shift, SDS-PAGE, and Western blotting of MAP kinase. Cells cultured in a 6-well plate were washed four times with serum- and NaHCO3-free DME suppl ...
... buffer for SDS-PAGE and Western blotting or in the assay buffer supplemented with 5 mM DTT for the cPLA2 activity assay. Phosphorylation-induced mobility shift, SDS-PAGE, and Western blotting of MAP kinase. Cells cultured in a 6-well plate were washed four times with serum- and NaHCO3-free DME suppl ...
Physiology of metabolic processes in the body. Composition of diet
... temperatures and so are at the mercy of the environment. In vertebrates, mechanisms for maintaining body temperature by adjusting heat production and heat loss have evolved. These species are called "coldblooded" (poikilothermic) because their body temperature fluctuates over a considerable range. I ...
... temperatures and so are at the mercy of the environment. In vertebrates, mechanisms for maintaining body temperature by adjusting heat production and heat loss have evolved. These species are called "coldblooded" (poikilothermic) because their body temperature fluctuates over a considerable range. I ...
213lec6
... challenging to meet their protein needs through only whole foods in the diet. As a result, supplementation may be warranted and thus beneficial. 4. Are there any risks associated with taking the protein supplement? Pay attention to ingredients lists because many protein supplements may also include ...
... challenging to meet their protein needs through only whole foods in the diet. As a result, supplementation may be warranted and thus beneficial. 4. Are there any risks associated with taking the protein supplement? Pay attention to ingredients lists because many protein supplements may also include ...
REGULATORY ENZYMES
... • Activation of zymogens by proteolytic cleavage result in irreversible activation. Zymogen forms allow proteins to be transported or stored in inactive forms that can be readily converted to active forms in response to some type of cellular signal. Thus they represent a mechanism whereby the levels ...
... • Activation of zymogens by proteolytic cleavage result in irreversible activation. Zymogen forms allow proteins to be transported or stored in inactive forms that can be readily converted to active forms in response to some type of cellular signal. Thus they represent a mechanism whereby the levels ...
pdf: Xu et al. 2008
... in Baskin, 2001), and the cellulose synthase rosette was found to move along the plasma membrane in tracks that largely coincided with the cortical microtubules (Paredez et al., 2006). Additional components involved in regulating cell wall biosynthesis have been identified in genetic screens for mut ...
... in Baskin, 2001), and the cellulose synthase rosette was found to move along the plasma membrane in tracks that largely coincided with the cortical microtubules (Paredez et al., 2006). Additional components involved in regulating cell wall biosynthesis have been identified in genetic screens for mut ...
Supporting Information S1 Metabolic Subsystems How the enzymes
... oscillations and quasi-steady state patterns can spontaneously emerge. In addition, the self-organization and the self-assembly processes may allow for reversible interactions between the multienzymatic system and other molecular structures, leading to the formation of a metabolic microcompartment, ...
... oscillations and quasi-steady state patterns can spontaneously emerge. In addition, the self-organization and the self-assembly processes may allow for reversible interactions between the multienzymatic system and other molecular structures, leading to the formation of a metabolic microcompartment, ...
Class: Protein functional Annotation and Family Classification
... Delineation of potential function(s) for distant paralogs Identification of domains in the absence of close homologs Analysis of proteins with low sequence complexity ...
... Delineation of potential function(s) for distant paralogs Identification of domains in the absence of close homologs Analysis of proteins with low sequence complexity ...
Product Datasheet
... Lysophosphatidic acid (LPA) is a lipid signalling molecule formed by the hydrolysis of lysophosphatidyl choline by lysophospholipase D, also known as autotaxin (ATX). LPA signals through four different G protein-coupled receptors, LPA1/EDG-2, LPA2/EDG-4, LPA3/EDG-7, and LPA4/GPR23. Activation of per ...
... Lysophosphatidic acid (LPA) is a lipid signalling molecule formed by the hydrolysis of lysophosphatidyl choline by lysophospholipase D, also known as autotaxin (ATX). LPA signals through four different G protein-coupled receptors, LPA1/EDG-2, LPA2/EDG-4, LPA3/EDG-7, and LPA4/GPR23. Activation of per ...
Slide 1
... intrinsically unfolded." Bioinformatics. 2005;21(16):3435-8, PMID: 15955783 Linding R, Russell RB, Neduva V, Gibson TJ. "GlobPlot: Exploring protein sequences for ...
... intrinsically unfolded." Bioinformatics. 2005;21(16):3435-8, PMID: 15955783 Linding R, Russell RB, Neduva V, Gibson TJ. "GlobPlot: Exploring protein sequences for ...
Review article Zinc finger protein (ZFP) in plants
... Meanwhile, ZFP belongs to a large family of transcription factor. The percentage of ZFP transcription factor in total transcription factors are obviously similar among plant species, suggested that ZFP is probably conserved in plant evolution. ZFP plays important role in plant, such as development, ...
... Meanwhile, ZFP belongs to a large family of transcription factor. The percentage of ZFP transcription factor in total transcription factors are obviously similar among plant species, suggested that ZFP is probably conserved in plant evolution. ZFP plays important role in plant, such as development, ...
PPT File
... 1. Prusiner S. provided evidence that the infectious agent has been traced to a single protein (Mr 28,000), prion (PrP). 2. Role of PrP: molecular signaling function in brain tissues 3. Strains of mice lacking the gene for PrP suffer no ill effects. 4. Illness occurs when the normal cellular PrPc oc ...
... 1. Prusiner S. provided evidence that the infectious agent has been traced to a single protein (Mr 28,000), prion (PrP). 2. Role of PrP: molecular signaling function in brain tissues 3. Strains of mice lacking the gene for PrP suffer no ill effects. 4. Illness occurs when the normal cellular PrPc oc ...
RED CELL MEMBRANE DEFECTS
... Aberrant interaction between the lipid bilayer and the skeleton Spectrin loss is caused by a defect in one of the membrane proteins involved in the attachment of spectrin to the membrane rather than a primary defect in the ...
... Aberrant interaction between the lipid bilayer and the skeleton Spectrin loss is caused by a defect in one of the membrane proteins involved in the attachment of spectrin to the membrane rather than a primary defect in the ...
Biochemistry
... Biochemistry and Molecular Biology is a required course for basic Medicine. It can be defined as the science to clarify the phenomenon and the nature of life and to explain the biochemistry problems about normal human and disease processes at the molecular level. Biochemistry and medicine are closel ...
... Biochemistry and Molecular Biology is a required course for basic Medicine. It can be defined as the science to clarify the phenomenon and the nature of life and to explain the biochemistry problems about normal human and disease processes at the molecular level. Biochemistry and medicine are closel ...
Eukaryotic-type protein kinases in Streptomyces
... enzymes. Their common feature seems to be a complex life cycle, including morphological and physiological differentiation, sophisticated cell communication, or interactions with host cells. The genome of Streptomyces coelicolor A3(2) (Bentley et al., 2002) was screened for the presence of ESTPK gene ...
... enzymes. Their common feature seems to be a complex life cycle, including morphological and physiological differentiation, sophisticated cell communication, or interactions with host cells. The genome of Streptomyces coelicolor A3(2) (Bentley et al., 2002) was screened for the presence of ESTPK gene ...
COMMUNICATION
... a subunit and 28 residues of Mac-1 a subunit were obquences of the murineLFA- 1 and Mac- 1 a subunits3 (16, tained (Fig. 2). The p150.95 and Mac-1 a subunits are 17).Our comparison withthe humanMac-1 and p150.95 54% identical, demonstrating a close relationship be- a subunit sequences demonstrates t ...
... a subunit and 28 residues of Mac-1 a subunit were obquences of the murineLFA- 1 and Mac- 1 a subunits3 (16, tained (Fig. 2). The p150.95 and Mac-1 a subunits are 17).Our comparison withthe humanMac-1 and p150.95 54% identical, demonstrating a close relationship be- a subunit sequences demonstrates t ...
Structure and function of proteins controlling strain
... The NBS-LRR class is by far the largest group of resistance proteins. Two subgroups within the NBS-LRR class have been recognised by the presence or absence of an amino-terminal region (TIR domain) with amino acid sequence similarity and predicted structural similarity [13•,14,15] to the cytoplasmic ...
... The NBS-LRR class is by far the largest group of resistance proteins. Two subgroups within the NBS-LRR class have been recognised by the presence or absence of an amino-terminal region (TIR domain) with amino acid sequence similarity and predicted structural similarity [13•,14,15] to the cytoplasmic ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.