• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
COMPARATIVE MODELING AND MOLECULAR
COMPARATIVE MODELING AND MOLECULAR

... In silico-developed homology protein structure modeling builds a threedimensional model of a given protein sequence based on its similarity to one or more known structures belonging to the same member of a protein family. The purpose of this study is strongly related to a drug discovery strategy aga ...
Name: JJJJJJJJJJJJJJJJJJJJ Student ID: JJJJJJJJJJJJJJJJJJJJJJJ
Name: JJJJJJJJJJJJJJJJJJJJ Student ID: JJJJJJJJJJJJJJJJJJJJJJJ

... Vmax = __~48-50 µM/min________ ...
Protein-Protein Interactions
Protein-Protein Interactions

... stronger than the interactions with water they make in the free proteins. In complexes of known 3D structure, the peptide group is part of at least half the hydrogen bonds at protein-protein interfaces. Side chain to main chain bonds are especially common, and main chain to main chain bonds do occur ...
Biol115_2014_Lecture 8_Protein Structure
Biol115_2014_Lecture 8_Protein Structure

... • Sickle-cell disease, an inherited blood disorder, results from a single amino acid substitution in the haemoglobin protein. ...
Document
Document

... These type of proteins are characterized by six transmembrane domains andtwo loops, B and E, that dip into the membrane from both sides, essentially forming a seventh transmembrane domain.  It appears that three regions play a role in Fps1 gating: the B loop, the region of about 40 amino acids prox ...
Macromolecules in your Food! – Info Sheet
Macromolecules in your Food! – Info Sheet

... Macromolecules: Macromolecules are very large molecules, formed of smaller subunits. In this lab, we will focus on the three macromolecules that are important energy sources for biological organisms: Carbohydrates, Proteins and Lipids. Carbohydrates: A carbohydrate is an organic compound that consi ...
Lecture 33
Lecture 33

... PPase = pyrophosphatase ...
Answer Set 1
Answer Set 1

... Why do some amino acids prefer one type of secondary structure over the other? The side chains of the amino acids lie up against the side of the α-helix, and serve to protect the core H-bonds from disruption by the surrounding H2O. α-Helix can become crowded when side chains are bulky or branch on t ...
RLIMS-P Annotation guidelines This document describes some
RLIMS-P Annotation guidelines This document describes some

... Kinase information: report kinase only when it is mentioned along with a substrate in the abstract. For example, if the abstract has a sentence of the type 'Y is a tyrosine kinase and here we show that it phosphorylates X', we would annotate Y as a kinase, and X as a substrate or site in substrate. ...
3D-structure of bacterial ribosomes, the machines that make
3D-structure of bacterial ribosomes, the machines that make

... most proteins don’t During production many proteins can only obtain correct folding if assisted by other proteins (chaperones) during production in living cells If large quantities of a specific chaperone-dependent protein is produced in a cell, it may become misfolded. This represents a very seriou ...
The rational drug design is one of the major challenges in structural
The rational drug design is one of the major challenges in structural

... Because conventional chemotherapy is not specific for cancer cells leading to toxic side effects there is a need for novel agents with high grade antitumor specificity. The major prerequisite to develop such drugs is to understand the targets that these agents should attack. In recent years a number ...
Understanding protein lists from comparative proteomics studies
Understanding protein lists from comparative proteomics studies

... Uniprot name is listed in column A and the gene name is listed in column R. If this is a time consuming task (and I imagine that it is), can you tell me how to do it?” From all human genes, selected those with the listed Uniprot IDs, and retrieve GO annotations. ...
Protein Structure Predictions 2
Protein Structure Predictions 2

... solved in different methods, under different conformation, through dynamics. ...
Bacterial Bioreactors for High Yield Production of Recombinant Protein
Bacterial Bioreactors for High Yield Production of Recombinant Protein

... FIGURE 1. A–D, pColdI(SP-2) and pColdI(SP-4) vectors support sustained, high level target protein expression. NdeI site that enables the in-frame Mature human eotaxin (A), HR91(B), EnvZB (C ), or HR969 (D) was expressed from pColdI(SP-2) (one ACA in 3⬘-UTR) or pColdI(SP-4) (no ACA in 3⬘-UTR) along w ...
Slide 1
Slide 1

... Cross your eyes, relax, & see if you can see how 2 molecules of steroid receptor, green & yellow, interact with a specific sequence, SRE, in DNA. ...
Budding yeast Saccharomyces cerevisiae as a model to study
Budding yeast Saccharomyces cerevisiae as a model to study

... some others that the identification of protein carbonyl levels as a marker of oxidative damage of proteins has both advantages and disadvantages. Certainly, the techniques based on this principle are most commonly used because they are well developed, reproducible, available and cheap. However, ther ...
The Body`s Building Blocks
The Body`s Building Blocks

... "The thing that scares me most about supplements is when people feed single amino acids," says Ralston. "I was advising one high-level competitor whose horses were tying-up, and found she was feeding a cup of lysine powder daily. She'd heard lysine was good, but this huge amount was causing great am ...
Sequence and Structural Similarities Between Glyceraldehyde
Sequence and Structural Similarities Between Glyceraldehyde

... Substrate-binding proteins are components of ATP-binding cassette transporters which capture their substrates in the periplasm of bacteria. These proteins subsequently deliver their bound ligands to membrane components of the transporters. Bacterial periplasmic substrate-binding proteins are charact ...
Identification of two novel mutations associated
Identification of two novel mutations associated

Paper - Journal of Environmental Biology
Paper - Journal of Environmental Biology

... important subcellular location prediction of newly identified proteins. Using N-terminal sequence information only, it categorize between proteins destined for the mitochondrion, the secretory pathway, the chloroplast, and "other" localizations with a achievement rate of 85% (plant) or 90% (non-plan ...
Structural Bioinformatics
Structural Bioinformatics

... One group dominates the ab initio (knowledge-based) prediction One lab dominated in CASP4 ...
Immunodetection of PR-1-Iike proteins in grapevine leaves infected
Immunodetection of PR-1-Iike proteins in grapevine leaves infected

Identification of cAMP-dependent phosphorylated proteins involved
Identification of cAMP-dependent phosphorylated proteins involved

Leukaemia Section t(11;14)(p13;q11), t(7;11)(q35;p13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(11;14)(p13;q11), t(7;11)(q35;p13) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Chromosomal breakpoints occur 25 kb upstream RBTN2 gene, in a presumed transcriptional start site, inducing truncation of the promoter/control region and leading to inappropriate Lmo2 level especially in T-cells (abnormal T-cell differentiation). ...
Amino acids and prot..
Amino acids and prot..

... hooves, or soft, as is the case with skin. It is difficult to dissolve keratin because it contains non polar amino acids plus cysteine, which means that it is able to form disulfide bridges. These bridges create a thread shape that is extremely strong, as sulfur atoms bond to each other, creating a ...
< 1 ... 93 94 95 96 97 98 99 100 101 ... 197 >

Protein purification



Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report