Biologically Assembled Nanobiocatalysts Heejae Kim Qing Sun
... several advantages. One major advantage is that biological assembly limits denaturation caused by immobilization as only properly folded enzymes are used. A proper orientation of the active site can also be controlled to maximize interactions with the substrate. Specific interactions and similar work ...
... several advantages. One major advantage is that biological assembly limits denaturation caused by immobilization as only properly folded enzymes are used. A proper orientation of the active site can also be controlled to maximize interactions with the substrate. Specific interactions and similar work ...
Document
... • Proteins are not linear structures, though they are built that way • The amino acids have very different chemical properties; they interact with each other after the protein is built • This causes the protein to start fold and adopting it’s functional structure • Proteins may fold in reaction to s ...
... • Proteins are not linear structures, though they are built that way • The amino acids have very different chemical properties; they interact with each other after the protein is built • This causes the protein to start fold and adopting it’s functional structure • Proteins may fold in reaction to s ...
Metabolic regulation of nitrogen fixation in Rhodospirillum rubrum
... Regulation of the DRAG/DRAT system These two enzymes are encoded by genes that are within the same operon (draT/draG/draB) and the expressed proteins have to work in a reciprocal manner; therefore it is postulated that both DRAG and DRAT themselves have to be posttranslationally regulated in vivo, b ...
... Regulation of the DRAG/DRAT system These two enzymes are encoded by genes that are within the same operon (draT/draG/draB) and the expressed proteins have to work in a reciprocal manner; therefore it is postulated that both DRAG and DRAT themselves have to be posttranslationally regulated in vivo, b ...
PROTEIN SUBCELLULAR LOCALIZATION - PolyU
... based methods could only deal with datasets containing a few subcellular locations. For example, the popular TargetP [13], [14] could only detect three locations: chloroplast, mitochondria and secretory pathway. Homology based methods, on the other hand, can detect as many locations as appeared in ...
... based methods could only deal with datasets containing a few subcellular locations. For example, the popular TargetP [13], [14] could only detect three locations: chloroplast, mitochondria and secretory pathway. Homology based methods, on the other hand, can detect as many locations as appeared in ...
G Protein Subunits Synthesized in Sf9 Cells
... data also suggest that the interaction of By with receptors is important for agonist-dependent receptor phosphorylation, suggesting an importantrole for Py in desensitization (8).By subunits can also regulate effector molecules. Genetic studies of Saccharomyces cerevisiae indicate that Py carries th ...
... data also suggest that the interaction of By with receptors is important for agonist-dependent receptor phosphorylation, suggesting an importantrole for Py in desensitization (8).By subunits can also regulate effector molecules. Genetic studies of Saccharomyces cerevisiae indicate that Py carries th ...
Biochemistry I, Spring Term 2005 - Second Exam:
... b) Drugs that are good inhibitors cannot by synthesized. c) The drugs are rapidly degraded. d) Virus particles with altered (mutant) proteases arise. 8. During any purification scheme, you would expect a) the number of different proteins in the sample to decrease. b) that the specific activity incre ...
... b) Drugs that are good inhibitors cannot by synthesized. c) The drugs are rapidly degraded. d) Virus particles with altered (mutant) proteases arise. 8. During any purification scheme, you would expect a) the number of different proteins in the sample to decrease. b) that the specific activity incre ...
The Case Against a Darwinian Origin of Protein Folds
... are really much simpler than they seem to be. It has to be said that the second option arouses immediate suspicion by disregarding matters of plain fact—both the actual properties of proteins and the cellular processes that only make sense if the first option is correct. This is admittedly more of a ...
... are really much simpler than they seem to be. It has to be said that the second option arouses immediate suspicion by disregarding matters of plain fact—both the actual properties of proteins and the cellular processes that only make sense if the first option is correct. This is admittedly more of a ...
Investigation of Iron-Sulfur Protein Maturation in Eukaryotes
... Fe-S proteins. The so-called mitochondrial iron-sulfur cluster (ISC) assembly machinery is responsible for the de novo synthesis of Fe-S clusters and the insertion of these cofactors into mitochondrial Fe-S apo-proteins. This system is also involved in the maturation of Fe-S proteins that are locate ...
... Fe-S proteins. The so-called mitochondrial iron-sulfur cluster (ISC) assembly machinery is responsible for the de novo synthesis of Fe-S clusters and the insertion of these cofactors into mitochondrial Fe-S apo-proteins. This system is also involved in the maturation of Fe-S proteins that are locate ...
Chapter 1
... medium or low resolution structures that are available from 2D and 3D crystals have revealed information on the size and shape of the proteins and the packing of α-helices (141, 153, 154, 159, 163). In the absence of high-resolution 3D structures, information on the structure of LacS has been obtain ...
... medium or low resolution structures that are available from 2D and 3D crystals have revealed information on the size and shape of the proteins and the packing of α-helices (141, 153, 154, 159, 163). In the absence of high-resolution 3D structures, information on the structure of LacS has been obtain ...
In Depth Analysis of Protein Amino Acid Sequence and PTMs with
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
Effect of
... values for Nile tilapia, Oreochromis niloticus (2.42-5.74 g; Brunty, 1997). Many factors may affect the TAN level including temperature, salinity, fish species, other water parameters, and experimental design. In the ornamental fish industry, water quality is very important to the health and quality ...
... values for Nile tilapia, Oreochromis niloticus (2.42-5.74 g; Brunty, 1997). Many factors may affect the TAN level including temperature, salinity, fish species, other water parameters, and experimental design. In the ornamental fish industry, water quality is very important to the health and quality ...
Bacteriophage lambda surface display of a bacterial biotin acceptor
... Due to the high a¤nity of biotin to avidin and streptavidin, biotin-based reagents and applications are widely used in molecular biology [1]. Examples include detection, localisation, puri¢cation and immobilisation of nucleic acids, proteins and other macromolecules. A large set of chemicals for the ...
... Due to the high a¤nity of biotin to avidin and streptavidin, biotin-based reagents and applications are widely used in molecular biology [1]. Examples include detection, localisation, puri¢cation and immobilisation of nucleic acids, proteins and other macromolecules. A large set of chemicals for the ...
S1.Cell Signaling-Introduction to the MAPK pathway
... 2. G proteins are described as molecular switches because: A. G proteins can be turned on and off by Sos. B. G proteins can activate or inhibit downstream components. C. G proteins can activate different downstream components depending on whether they are bound to GDP or GTP. D. G proteins have inhe ...
... 2. G proteins are described as molecular switches because: A. G proteins can be turned on and off by Sos. B. G proteins can activate or inhibit downstream components. C. G proteins can activate different downstream components depending on whether they are bound to GDP or GTP. D. G proteins have inhe ...
Protein thermal stability: insights from atomic displacement
... positions due to thermal motion and positional disorder. Analysis of B values, therefore, is likely to provide newer insights into protein dynamics, flexibility of amino acids and protein stability. Molecular dynamics studies have suggested that protein unfolding might be initiated at sites that are ...
... positions due to thermal motion and positional disorder. Analysis of B values, therefore, is likely to provide newer insights into protein dynamics, flexibility of amino acids and protein stability. Molecular dynamics studies have suggested that protein unfolding might be initiated at sites that are ...
Proc. Natl. Acad. Sci. USA 96, 4718-4723.
... a template. One of the primer pairs (PCD1, 59-ATCTA(Cy T)GA(CyT)ATGGAN-39; PCD2, 59-(AyC)(AyC)CIAATIGTCTT(CyT)TCN-39 yielded a PCR product of the expected size (shown in Fig. 1A). The PCR products were gel purified and cloned into PCRII TA vector as described by the manufacturer (Invitrogen). A 1- t ...
... a template. One of the primer pairs (PCD1, 59-ATCTA(Cy T)GA(CyT)ATGGAN-39; PCD2, 59-(AyC)(AyC)CIAATIGTCTT(CyT)TCN-39 yielded a PCR product of the expected size (shown in Fig. 1A). The PCR products were gel purified and cloned into PCRII TA vector as described by the manufacturer (Invitrogen). A 1- t ...
Possibilities for feeding low-protein diets to weanling and growing pigs
... AA for maintenance rather than lean protein synthesis. The ideal ratio for maintenance is different from the ideal ratio for lean meat production, and as a greater proportion of the dietary AA are used for maintenance, the ideal profile of the entire diet will change. Once the ideal ratios have been ...
... AA for maintenance rather than lean protein synthesis. The ideal ratio for maintenance is different from the ideal ratio for lean meat production, and as a greater proportion of the dietary AA are used for maintenance, the ideal profile of the entire diet will change. Once the ideal ratios have been ...
Developmental Analysis of a Putative ATP/ADP Carrier Protein
... et al. 1968). Peroxisomes are transformed from glyoxysomes to leaf peroxisomes in greening cotyledons of fatty seedlings, and this transformation is controlled by light (Nishimura et al. 1993, Titus and Becker 1985). The reverse conversion from leaf peroxisomes to glyoxysomes also occurs in senescin ...
... et al. 1968). Peroxisomes are transformed from glyoxysomes to leaf peroxisomes in greening cotyledons of fatty seedlings, and this transformation is controlled by light (Nishimura et al. 1993, Titus and Becker 1985). The reverse conversion from leaf peroxisomes to glyoxysomes also occurs in senescin ...
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... immune function, lean muscle mass, and a good supply of energy. Protein is most commonly thought of as being in meats, fishes, and poultries, but it can also be found in cheese, milk, nuts, legumes, and even in some vegetables! Upon consuming any of these foods that are excellent sources of protein, ...
... immune function, lean muscle mass, and a good supply of energy. Protein is most commonly thought of as being in meats, fishes, and poultries, but it can also be found in cheese, milk, nuts, legumes, and even in some vegetables! Upon consuming any of these foods that are excellent sources of protein, ...
Comparative Visualization of Protein Structure
... scientists have gained access to tremendous amounts of biological information. Due to the difficulties inherent in understanding large quantities of data, the field of bioinformatics is becoming an attractive target for the application of visualization techniques. [8] [9] Using information visualiza ...
... scientists have gained access to tremendous amounts of biological information. Due to the difficulties inherent in understanding large quantities of data, the field of bioinformatics is becoming an attractive target for the application of visualization techniques. [8] [9] Using information visualiza ...
... Choice B: Poly-lysine is a long polypeptide that contains only lysine residue, the sidechain of lysine is shown on the right. Poly-lysine strongly adheres to negatively charged surfaces at low pH values (e.g. pH < 6). However, its adherence properties become C α progressively weaker as the pH is rai ...
Local protein synthesis in neuronal axons: why and
... two compartments can be achieved, simply by adjusting the volume of culture medium in each compartment. For example, when more culture medium is added to the proximal compartment, any chemical treated into the distal compartment will remain there because of the negative hydrostatic pressure in the m ...
... two compartments can be achieved, simply by adjusting the volume of culture medium in each compartment. For example, when more culture medium is added to the proximal compartment, any chemical treated into the distal compartment will remain there because of the negative hydrostatic pressure in the m ...
Thermal unfolding of proteins at high pH range studied by UV
... protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used ...
... protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used ...
Thermostability of the human respiratory syncytial virus fusion
... FD1–109 and F2* intermediates were cleaved at the remaining furin sites, whereas the F1 polypeptide remained essentially unchanged, although low levels of new bands moving faster than F1 were also observed (Fig. 1b). To test the effect of trypsin on the aggregated state of FTM{ and D137–146, the two ...
... FD1–109 and F2* intermediates were cleaved at the remaining furin sites, whereas the F1 polypeptide remained essentially unchanged, although low levels of new bands moving faster than F1 were also observed (Fig. 1b). To test the effect of trypsin on the aggregated state of FTM{ and D137–146, the two ...
Domain structure and sequence similarities in cartilage proteoglycan
... protein, taken together for all four species determined, show a similarity of about 48%, while that of the B’ loops is 35%. For link protein alone, comparison among different species shows the amino acid sequence similarity between these two loops to be almost 60%. The G1 domain has been shown to bi ...
... protein, taken together for all four species determined, show a similarity of about 48%, while that of the B’ loops is 35%. For link protein alone, comparison among different species shows the amino acid sequence similarity between these two loops to be almost 60%. The G1 domain has been shown to bi ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.