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Sequence analysis of the Marburg virus nucleoprotein gene
Sequence analysis of the Marburg virus nucleoprotein gene

... Fig. 2. Specificity of cDNA clones, MBG mRNAs and sequencing of the 5' end of the MBG NP mRNA. (a) Northern blot hybridization of 32P-labelled probes (nick translation) generated from eDNA clones MV-88 and MV-17 (see Fig. 1) to lanes of RNA resolved by electrophoresisin an acid-urea-agarose (1-5~) g ...
Cloning and characterization of a phosphopantetheinyl transferase
Cloning and characterization of a phosphopantetheinyl transferase

... attachment of the P-pant group to a conserved serine residue of the carrier proteins. This posttranslational modi¢cation is catalyzed by a superfamily of enzymes known as phosphopantetheinyl transferases (PPTases) that derive the P-pant group from coenzyme A (CoA) [9,10]. In organisms containing mul ...
Objectives 30 - u.arizona.edu
Objectives 30 - u.arizona.edu

... • NADPH for lipogenesis is derived from malic enzyme and the pentose phosphate pathway • acetyl CoA carboxylase converts acetyl CoA to malonyl CoA in a biotinrequiring reaction • fatty acid synthase progressively adds two carbon units, from malonyl CoA, to a growing acyl chain to form the 16-carbon ...
ap sample5lab2 - Biology Junction
ap sample5lab2 - Biology Junction

... uncatalyzed reaction. Place 10-15mL of H2O2 in a 50mL beaker and store it uncovered for approximately 24 hours Determine the amount of H2O2 remaining after 24 hours by running a baseline test. Use a 10mL syringe to get 10mL of 2% KMnO4. Add the KMnO4 to the solution one drop at a time (swirling afte ...
Phar 722 Pharmacy Practice III
Phar 722 Pharmacy Practice III

... – In infants there is a characteristic type of convulsions which is reversible when pyridoxine supplements are given. – Deficient infants also show a characteristic electrical encephalogram. (This was "discovered" when infants were fed an infant formula lacking pyridoxine.) ...
metabolism during adventitious root primordium initiation
metabolism during adventitious root primordium initiation

... 1971; Nanda, Anand, Kochlar and Jain, 1971). Applied simple sugars have no or an inhibitory effect under conditions of endogenous carbohydrate sufficiency (Lovell, Cobb and Moore, 1971; Lovell, Illsley and Moore, 1972; Moore, Cobb and Lovell, 1972). Enhanced starch metabolism in cuttings probably re ...
Medical Endocrine Course
Medical Endocrine Course

... Understand therapeutic goal of Type I diabetes how to use the drugs to treat it. Know the different delivery methods for insulin and the side effects associated with them. Understand therapeutic goal of Type II diabetes and how to use the drugs to treat it. Know the factors to consider in the treatm ...
Increased Lysine Synthesis Coupled with a Knockout of
Increased Lysine Synthesis Coupled with a Knockout of

... therefore, they are considered of great nutritional importance in animal feeds and human foods (Galili et al., 1994). These three essential amino acids are synthesized in plants from Asp via several different branches of the Asp family pathway (Figure 1). Asp is synthesized either from Gln via Glu o ...
Archive Microbiology
Archive Microbiology

... demonstrated in Escherichia coli, Azotobacter, Micrococcus and the blue-green alga Anacystis nidulans, and is likely present in the green algae Ankistrodesmus and Chlamydomonas. In conclusion, in those organisms tested, a low level of lipoic acid is a sufficient precondition for the occurrence of th ...
Acid – base balance
Acid – base balance

... All the known low molecular weight and water soluble biosynthetic intermediates possess groups that are almost completely ionised at neutral pH’ ...
Universal Functional and Model Consistency Testing
Universal Functional and Model Consistency Testing

... Amino Acid Production Tests Amino acid production was an important process for each network as these compounds were used as part of the cell specific biomass equations as well as alternative sources of energy in some of the networks. Of the 20 amino acids, there are 9 essential amino acids that can ...
Glutathione and glutamate levels in the diaphragm of patients with
Glutathione and glutamate levels in the diaphragm of patients with

... chromatography (HPLC) [25, 26]. Levels of the following amino acids were measured: glutamine, glycine, alanine, valine, isoleucine, leucine, phenylalanine, tyrosine, arginine, histidine, lysine, methionine, threonine, tryptophan, alphaaminobutyric acid, asparagine, citrulline, Glu, ornithine, serine ...
Haem biosynthesis and excretion of porphyrins
Haem biosynthesis and excretion of porphyrins

PCTPC201500105RAR1_pap_plantcell 1..17
PCTPC201500105RAR1_pap_plantcell 1..17

... Mitochondrial dihydrolipoyl dehydrogenase (mtLPD; L-protein) is an integral component of several multienzyme systems involved in the tricarboxylic acid (TCA) cycle, photorespiration, and the degradation of branched-chain a-ketoacids. The majority of the mtLPD present in photosynthesizing tissue is u ...
to this tutorial as a PDF
to this tutorial as a PDF

... can begin to link together selections using Boolean Operators. To understand how boolean operators work, imagine that you are working with two predefined selection types, helices and backbone. These two selection types are shown in a Venn diagram below. Some atoms in our Top 7 protein are part of he ...
Purification and Partial Characterization of a Latent Serine Protease
Purification and Partial Characterization of a Latent Serine Protease

... During purification of proteases Mi and Fa, we fortuitously isolated a new, additional serine protease (LSP) in E. coli. From 8 g of the crude extract, approximately 1 flg of the purified LSP was obtained. However, the final yield as well as -·the specific activity could not be calculated until the ...
chapter 9 cellular respiration: harvesting chemical energy
chapter 9 cellular respiration: harvesting chemical energy

... A more efficient and widespread catabolic process, cellular respiration, consumes oxygen as a reactant to complete the breakdown of a variety of organic molecules. ...
scheme of work biology lower six - laman web smk raja perempuan
scheme of work biology lower six - laman web smk raja perempuan

... 2)to compare different types of protein 3)to understand the sequencing of amino acids in determining the properties of protein 4)to elaborate the properties of protein;amphoteric,buffer,colloid,denaturation of protein 5)to give examples of fibrous and globular ...
A modular approach to sphingolipid analogs mediated by aziridines: Synthesis
A modular approach to sphingolipid analogs mediated by aziridines: Synthesis

... Sphingolipid  structures  are  defined  by  their  eighteen  carbon  backbones  with  a  2‐amino‐1,3‐ diol functionality (usually 2S, 3R), which are called sphingoid bases. These organic bases can be  N‐acylated  by  fatty  acids  of  different  length  giving  ceramides.  Modification  of  this  ge ...
The Effect of Temperature on the Metabolism of
The Effect of Temperature on the Metabolism of

... conditions in the respirometer, the carbon dioxide output was found to be higher as temperature at which the organisms were cultured increased from 25 to 39": in contrast, the oxygen uptake remained fairly constant between 25 and 32" and then fell steadily with increasing temperature (Fig. 3). The r ...
Cell Respiration
Cell Respiration

... A more efficient and widespread catabolic process, cellular respiration, consumes oxygen as a reactant to complete the breakdown of a variety of organic molecules. ...
gsridhara_1
gsridhara_1

... – have amino acid frequencies as in the columns of a MSA – Also have position specific probabilities for inserts and deletions along the alignment – logarithms of these probabilities =position specific gap penalties – Perform better than sequence profiles in the detection of homologs and in the qual ...
Protein and vegetarian diets - Medical Journal of Australia
Protein and vegetarian diets - Medical Journal of Australia

... Newcastle, NSW. ...
Pyruvate Dehydrogenase
Pyruvate Dehydrogenase

...  Pyruvate Dehydrogenase Kinases catalyze phosphorylation of serine residues of E1, inhibiting the complex.  Pyruvate Dehydrogenase Phosphatases reverse this inhibition. Pyruvate Dehydrogenase Kinases are activated by NADH & acetyl-CoA, providing another way the 2 major products of Pyruvate Dehydro ...
All fatty acids are not equal: discrimination in plant membrane lipids
All fatty acids are not equal: discrimination in plant membrane lipids

... These fatty acids are often referred to as common fatty acids. In contrast with the conservative fatty acid composition of plant membrane lipids, tremendous fatty acid diversity exists in the seed storage lipids. To date, .300 naturally occurring fatty acids have been described in seed oils1,2, and ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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