Biotechnology and Bioengineering
... using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F196-rear, and a 898 bp DNA fragment was amplified using primers F196-front and ToMO-SalI-rear. The two deg ...
... using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F196-rear, and a 898 bp DNA fragment was amplified using primers F196-front and ToMO-SalI-rear. The two deg ...
ribosomal defects in a mutant deficient in the yajl homolog of the
... determination of acetate, lactate, ethanol, succinate and formate. These determinations were made by using the corresponding Megazyme assay kits for acetic acid, lactic acid, ethanol, acetic acid and formic acid, following instructions of the manufacturer (Megazyme International Ireland Limited). Th ...
... determination of acetate, lactate, ethanol, succinate and formate. These determinations were made by using the corresponding Megazyme assay kits for acetic acid, lactic acid, ethanol, acetic acid and formic acid, following instructions of the manufacturer (Megazyme International Ireland Limited). Th ...
Enzyme promiscuity: evolutionary and mechanistic aspects
... The above observation has important implications regarding our understanding of the early steps in the evolution of new protein functions [37]. There is no doubt that gene duplication is a necessary step. But the most-widely accepted model, by Ohno [38], surmises that the generation of a redundant g ...
... The above observation has important implications regarding our understanding of the early steps in the evolution of new protein functions [37]. There is no doubt that gene duplication is a necessary step. But the most-widely accepted model, by Ohno [38], surmises that the generation of a redundant g ...
Molecular cloning of a cDNA encoding a novel Ca2+
... Fig. 1. Nucleotide and deduced amino acid sequences of the cloned cDNA. The nucleotides are numbered from the ¢rst nucleotide of the initiation codon. The arrows indicate synthetic primers used for RT^PCR. The sequence underlined is the recognition site of EcoRI. Total cellular DNA was extracted wit ...
... Fig. 1. Nucleotide and deduced amino acid sequences of the cloned cDNA. The nucleotides are numbered from the ¢rst nucleotide of the initiation codon. The arrows indicate synthetic primers used for RT^PCR. The sequence underlined is the recognition site of EcoRI. Total cellular DNA was extracted wit ...
Mechanism of Succinyl
... reaction far towards product – Unfortunately the resulting citrate is a tertiary alcohol which cannot be readily oxidized ...
... reaction far towards product – Unfortunately the resulting citrate is a tertiary alcohol which cannot be readily oxidized ...
The Citric Acid Cycle
... allosteric interactions and covalent modifications. As stated earlier, glucose can be formed from pyruvate through the gluconeogenic pathway (p. 251). However, the formation of acetyl CoA from pyruvate is an irreversible step in animals and thus they are unable to convert acetyl CoA back into glucos ...
... allosteric interactions and covalent modifications. As stated earlier, glucose can be formed from pyruvate through the gluconeogenic pathway (p. 251). However, the formation of acetyl CoA from pyruvate is an irreversible step in animals and thus they are unable to convert acetyl CoA back into glucos ...
biochem_lec3_27-4
... *Slide (4) These are the gall bladder stones, they are made of cholesterol so cholesterol may precipitate in the gall bladder forming cholesterol stones that’s because its is poorly or insoluble in water and its secreted in the bile from where it was isolated first. It should be in the soluble fo ...
... *Slide (4) These are the gall bladder stones, they are made of cholesterol so cholesterol may precipitate in the gall bladder forming cholesterol stones that’s because its is poorly or insoluble in water and its secreted in the bile from where it was isolated first. It should be in the soluble fo ...
Metabolism, Energy Balance, and Body Composition © 2009 Cengage - Wadsworth
... Glucose and fatty acids are primarily used for energy, amino acids to a lesser extent. Glucose is made from all carbohydrates, most amino acids and the glycerol portion of fat. Protein is made from amino acids. Glucose can be made into nonessential amino acids if nitrogen is present. All e ...
... Glucose and fatty acids are primarily used for energy, amino acids to a lesser extent. Glucose is made from all carbohydrates, most amino acids and the glycerol portion of fat. Protein is made from amino acids. Glucose can be made into nonessential amino acids if nitrogen is present. All e ...
Title: Hormones of the Pancreas
... 4. Insulin receptor: membrane receptors consist of 2α and 2β units, receptor spans the membrane, α subunit binds insulin , β subunit has tyrosine kinase activity on the cytosolic side, hormone - receptor complex is internalized by endocytosis, number of receptors is regulated, when occupied by insul ...
... 4. Insulin receptor: membrane receptors consist of 2α and 2β units, receptor spans the membrane, α subunit binds insulin , β subunit has tyrosine kinase activity on the cytosolic side, hormone - receptor complex is internalized by endocytosis, number of receptors is regulated, when occupied by insul ...
Title: Hormones of the Pancreas
... 4. Insulin receptor: membrane receptors consist of 2α and 2β units, receptor spans the membrane, α subunit binds insulin , β subunit has tyrosine kinase activity on the cytosolic side, hormone - receptor complex is internalized by endocytosis, number of receptors is regulated, when occupied by insul ...
... 4. Insulin receptor: membrane receptors consist of 2α and 2β units, receptor spans the membrane, α subunit binds insulin , β subunit has tyrosine kinase activity on the cytosolic side, hormone - receptor complex is internalized by endocytosis, number of receptors is regulated, when occupied by insul ...
PPTX - Bonham Chemistry
... • Pyruvate can be a source of new glucose – Store energy as glycogen – Generate NADPH via pentose phosphate pathway • Pyruvate can be a source of acetyl-CoA – Store energy as body fat – Make ATP via citric acid cycle • Acetyl-CoA stimulates glucose synthesis by activating pyruvate carboxylase ...
... • Pyruvate can be a source of new glucose – Store energy as glycogen – Generate NADPH via pentose phosphate pathway • Pyruvate can be a source of acetyl-CoA – Store energy as body fat – Make ATP via citric acid cycle • Acetyl-CoA stimulates glucose synthesis by activating pyruvate carboxylase ...
Fluorescence Study of Bovine β-Lactoglobulin
... spectra (in absence and presence of calcofluor white) yields an emission spectrum with a peak located at 332 nm and not at 340 or 345 nm, an emission peak characteristic of Trp residue present at the protein surface. This result clearly indicates that Trp 61 residue does not contribute to the protei ...
... spectra (in absence and presence of calcofluor white) yields an emission spectrum with a peak located at 332 nm and not at 340 or 345 nm, an emission peak characteristic of Trp residue present at the protein surface. This result clearly indicates that Trp 61 residue does not contribute to the protei ...
carbohydrate metabolism
... aldohexose. It is oxidized by hot alkaline copper solution, potassium ferricyanide solution. These methods give 10-20 mg higher values because in the blood there are other reducing substances (gluthathion, ascorbic acid). Colorimetric methods are rapid and based on the reaction between the glucose a ...
... aldohexose. It is oxidized by hot alkaline copper solution, potassium ferricyanide solution. These methods give 10-20 mg higher values because in the blood there are other reducing substances (gluthathion, ascorbic acid). Colorimetric methods are rapid and based on the reaction between the glucose a ...
CITRIN DEFICIENCY
... metabolic disease caused by mutations in the SLC25A13 gene encoding mitochondrial transporter citrin. It causes a different clinical presentation regarding to age, from neonatal cholestasis (impaired liver function) to citrullinemia (elevated levels of citrulline in blood) in adults. Citrin deficien ...
... metabolic disease caused by mutations in the SLC25A13 gene encoding mitochondrial transporter citrin. It causes a different clinical presentation regarding to age, from neonatal cholestasis (impaired liver function) to citrullinemia (elevated levels of citrulline in blood) in adults. Citrin deficien ...
my handy vitamin review
... Vitamin D binds to a “vitamin D binding protein” (VDP) for transport to target organs. Vitamin D is not active itself (it’s a prohormone); it is modified to yield biologically active forms, such as calcitriol. Calcitriol (derived from vitamin D) is a transcription factor, influencing expression of ...
... Vitamin D binds to a “vitamin D binding protein” (VDP) for transport to target organs. Vitamin D is not active itself (it’s a prohormone); it is modified to yield biologically active forms, such as calcitriol. Calcitriol (derived from vitamin D) is a transcription factor, influencing expression of ...
INTERORGAN AMMONIA TRAFFICKING
... • Liver has key function in nitrogen metabolism • Ammonia is important in several biochemical pathway but toxic at elevated level. • Ammonia is central in mechanism of encephalopathy in liver failure • Normally ammonia is present at nontoxic level • Hyperammonia in chronic liver disease is adapted i ...
... • Liver has key function in nitrogen metabolism • Ammonia is important in several biochemical pathway but toxic at elevated level. • Ammonia is central in mechanism of encephalopathy in liver failure • Normally ammonia is present at nontoxic level • Hyperammonia in chronic liver disease is adapted i ...
OptCDR: a general computational method for the design
... penalized with a negative score. Alternatively, if the two atoms are more than 8 Å apart, then the contact is considered to be unlikely to contribute to binding and a zero score is assigned. Only if the distance is between the sum of the two atoms Van der Waals radii and 8 Å is a positive score as ...
... penalized with a negative score. Alternatively, if the two atoms are more than 8 Å apart, then the contact is considered to be unlikely to contribute to binding and a zero score is assigned. Only if the distance is between the sum of the two atoms Van der Waals radii and 8 Å is a positive score as ...
Metabolic reaction network approach for CHO
... evaluations of cellular function and a better control over physiological responsiveness [7]. There are some aspects that must be taken into account in the design of serum-free media. The first one is that it does not exist a universal media that can be used in all cell types; so different serum-free ...
... evaluations of cellular function and a better control over physiological responsiveness [7]. There are some aspects that must be taken into account in the design of serum-free media. The first one is that it does not exist a universal media that can be used in all cell types; so different serum-free ...
Reduced lipid intake leads to changes in - Archimer
... PUFA contents are affected in Atlantic salmon (Salmo salar) (Torstensen et al., 2000; Bell et al., 2001 and 2002; Stubhaug et al., 2005). However, using a fish oil ‘finishing diet’ at the end of the experiment can partially restore the EPA and DHA levels in fish flesh (Bell et al., 2003 and 2004; To ...
... PUFA contents are affected in Atlantic salmon (Salmo salar) (Torstensen et al., 2000; Bell et al., 2001 and 2002; Stubhaug et al., 2005). However, using a fish oil ‘finishing diet’ at the end of the experiment can partially restore the EPA and DHA levels in fish flesh (Bell et al., 2003 and 2004; To ...
18. enzymes iii
... Frederick August Kekule (LT, 1829-1867), master of structural chemistry. After receiving his doctoral degree from the University of Strasbourg in 1874, he taught at the Universities of Erlangen and Wurzburg, eventually becoming Professor of Chemistry at the University of Berlin in 1892 while succeed ...
... Frederick August Kekule (LT, 1829-1867), master of structural chemistry. After receiving his doctoral degree from the University of Strasbourg in 1874, he taught at the Universities of Erlangen and Wurzburg, eventually becoming Professor of Chemistry at the University of Berlin in 1892 while succeed ...
Protein proteinase inhibitors from avian egg whites
... The ability of chicken egg white to inhibit trypsin was recognized in the early part of the twentieth century [1] and was attributed to ovomucoid [2]. At present, three different proteins of the avian egg white are known to possess trypsin inhibitory activity: ovomucoid, ovoinhibitor and ovostatin [ ...
... The ability of chicken egg white to inhibit trypsin was recognized in the early part of the twentieth century [1] and was attributed to ovomucoid [2]. At present, three different proteins of the avian egg white are known to possess trypsin inhibitory activity: ovomucoid, ovoinhibitor and ovostatin [ ...
document
... acids (monomers), cells use a hydrolysis reaction a protein reacts with a water molecule to produce an amino acid and a protein 7 Carboxypeptidase is secreted by the pancreas, and speeds up the hydrolysis reaction between the protein and the water molecule 8 The main products of this hydrolysis re ...
... acids (monomers), cells use a hydrolysis reaction a protein reacts with a water molecule to produce an amino acid and a protein 7 Carboxypeptidase is secreted by the pancreas, and speeds up the hydrolysis reaction between the protein and the water molecule 8 The main products of this hydrolysis re ...
Ch. 03 The Molecules of Life
... • All proteins are constructed from a common set of 20 kinds of amino acids • Each amino acid : – A central carbon atom bonded to four covalent partners ...
... • All proteins are constructed from a common set of 20 kinds of amino acids • Each amino acid : – A central carbon atom bonded to four covalent partners ...
lecture6
... l -3-Hydroxyacyl CoA + NAD+ ↔ 3- keto acyl CoA + NADH+ H+ 3-ketoacyl CoA + CoA ↔acetyl CoA + acyl CoA (shortened by C2) ...
... l -3-Hydroxyacyl CoA + NAD+ ↔ 3- keto acyl CoA + NADH+ H+ 3-ketoacyl CoA + CoA ↔acetyl CoA + acyl CoA (shortened by C2) ...