REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... acyltransferase family. However, the recent report of the 3-D structures of CrAT and COT has provided valuable insights into the molecular basis of substrate specificity and catalytic activity in the acyltransferase family (Jogl 2003, Jogl 2005). ...
... acyltransferase family. However, the recent report of the 3-D structures of CrAT and COT has provided valuable insights into the molecular basis of substrate specificity and catalytic activity in the acyltransferase family (Jogl 2003, Jogl 2005). ...
From Sequence to Structure
... with each other and with water. These differences profoundly influence their contributions to protein stability and to protein function. Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for ...
... with each other and with water. These differences profoundly influence their contributions to protein stability and to protein function. Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for ...
Cloning and functional expression of invertebrate connexins from
... in the extracellular domains, we compared the Hp Cx47 sequence with Ci Cx36.9, Ci Cx37.3, Ci Cx46.7 and Ci Cx50.6. Like Hp Cx47, these Ciona connexins contained all of the typical connexin features, including two highly conserved extracellular domains each containing three invariant cysteines (Fig. ...
... in the extracellular domains, we compared the Hp Cx47 sequence with Ci Cx36.9, Ci Cx37.3, Ci Cx46.7 and Ci Cx50.6. Like Hp Cx47, these Ciona connexins contained all of the typical connexin features, including two highly conserved extracellular domains each containing three invariant cysteines (Fig. ...
A novel assay method for an amino acid racemase reaction based
... when the ALR activity from D-CS-producing S. lavendulae was measured [22]. It is significant that D-CS inhibits the catalytic activity of D-amino acid oxidase, which is contained in the reaction mixture for the enzyme-coupled assay [29]. These observations made it possible to develop another method ...
... when the ALR activity from D-CS-producing S. lavendulae was measured [22]. It is significant that D-CS inhibits the catalytic activity of D-amino acid oxidase, which is contained in the reaction mixture for the enzyme-coupled assay [29]. These observations made it possible to develop another method ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... n 20 naturally occurring amino acids in proteins n Non-polar n G glycine, A alanine, P proline, V valine n I isoleucine, L leucine, F phenylalanine, M methionine n Polar n S serine, C cysteine, T threonine, N asparagine n Q glutamine, H histidine, Y tyrosine, W tryptophan n Charged n D aspartic acid ...
... n 20 naturally occurring amino acids in proteins n Non-polar n G glycine, A alanine, P proline, V valine n I isoleucine, L leucine, F phenylalanine, M methionine n Polar n S serine, C cysteine, T threonine, N asparagine n Q glutamine, H histidine, Y tyrosine, W tryptophan n Charged n D aspartic acid ...
Correlation between the respiration-driven Na+ pump
... Except for the concentration of NaCl required for optimal growth (1.0-2.0 M), the six Gram-negative moderate halophiles examined are very similar to the marine bacteria in the following properties: (1) the NaC1-washed cells quickly lyse in a hypotonic medium; (2) they have Na+-dependentNADH-quinone ...
... Except for the concentration of NaCl required for optimal growth (1.0-2.0 M), the six Gram-negative moderate halophiles examined are very similar to the marine bacteria in the following properties: (1) the NaC1-washed cells quickly lyse in a hypotonic medium; (2) they have Na+-dependentNADH-quinone ...
Analysis of single nucleotide polymorphisms in human
... Correlation with predicted secondary structure Secondary structure predictions of the exon sequences containing the SNPs show that SNPs are found in all the secondary structural elements: helical, beta-sheet and coil regions. While the absolute number of SNPs in coils is generally more than the numb ...
... Correlation with predicted secondary structure Secondary structure predictions of the exon sequences containing the SNPs show that SNPs are found in all the secondary structural elements: helical, beta-sheet and coil regions. While the absolute number of SNPs in coils is generally more than the numb ...
Amino Acids in Maturation Medium and Presence
... of porcine oocytes are yet to be established. Furthermore, there is little information about the factors affecting MPN formation with relation to the culture condition. Therefore, further studies of the effects of individual factors in media on maturation and fertilization of porcine oocytes in vitr ...
... of porcine oocytes are yet to be established. Furthermore, there is little information about the factors affecting MPN formation with relation to the culture condition. Therefore, further studies of the effects of individual factors in media on maturation and fertilization of porcine oocytes in vitr ...
Cis-trans isomerization of omega dihedrals in Proteins
... Proteins are major components of all cells. Composed of a sequence of amino acids, they are the support of critical biological functions. Amino acids are composed of a succession of N, Cα, C and O atoms defining the protein backbone while more versatile side-chains give them specific physiochemical ...
... Proteins are major components of all cells. Composed of a sequence of amino acids, they are the support of critical biological functions. Amino acids are composed of a succession of N, Cα, C and O atoms defining the protein backbone while more versatile side-chains give them specific physiochemical ...
Feodor Lynen - Nobel Lecture
... It was possible to assume with fair certainty from these results that the succinic acid produced by yeast from acetate is formed via citric acid7. Sonderhoff’s experiments with deuterated acetic acid led to another important discovery. In the analysis of the yeast cells themselves, it was found that ...
... It was possible to assume with fair certainty from these results that the succinic acid produced by yeast from acetate is formed via citric acid7. Sonderhoff’s experiments with deuterated acetic acid led to another important discovery. In the analysis of the yeast cells themselves, it was found that ...
3.1.molecular_evolution - T
... (or “clocklike”) and because selection does not influence the rate of divergence, divergence of DNA and protein molecules in two separate lineages should occur in a REGULAR, clocklike manner ...
... (or “clocklike”) and because selection does not influence the rate of divergence, divergence of DNA and protein molecules in two separate lineages should occur in a REGULAR, clocklike manner ...
Bioinfo primer - part 4/6
... • Aligning 2 sequences along their whole length is done by stepping through the matrix from top left to bottom right. The best-scoring path can be traced through the matrix, resulting in an optimal alignment. The NeedlemanWunsch algorithm belongs to this class. • Sequences are often modular, therefo ...
... • Aligning 2 sequences along their whole length is done by stepping through the matrix from top left to bottom right. The best-scoring path can be traced through the matrix, resulting in an optimal alignment. The NeedlemanWunsch algorithm belongs to this class. • Sequences are often modular, therefo ...
biosynthesis of fatty acids - Academic Research Collections
... because they can make it if P-aminobenzoic acid (PABA), one of the components of folic acid, is available. Thus PABA is a vitamin for these bacteria. If sulphanilamide is administered, it competes with PABA in the enzymatic synthesis of folic acid due to its structural similarity and inhibits the gr ...
... because they can make it if P-aminobenzoic acid (PABA), one of the components of folic acid, is available. Thus PABA is a vitamin for these bacteria. If sulphanilamide is administered, it competes with PABA in the enzymatic synthesis of folic acid due to its structural similarity and inhibits the gr ...
The different roles of tryptophan transfer RNA in regulating trp
... their respective tRNAs. Amino acids are provided by biosynthesis, transport from the environment and/or degradation of existing proteins. Charging of their respective tRNAs is catalyzed by tRNA synthetases, whose properties and cellular levels determine their ability to provide sufficient levels of ...
... their respective tRNAs. Amino acids are provided by biosynthesis, transport from the environment and/or degradation of existing proteins. Charging of their respective tRNAs is catalyzed by tRNA synthetases, whose properties and cellular levels determine their ability to provide sufficient levels of ...
Identification of functional domains in Arabidopsis thaliana mRNA
... region for preliminary binding. In addition, uncapped transcripts are efficient competitors for capped RNA, suggesting that binding of mRNA to hDcp2 is independent of the cap itself (17). A region essential for RNA binding by the hDcp2 protein was demonstrated by deletion mutagenesis. The deleted regi ...
... region for preliminary binding. In addition, uncapped transcripts are efficient competitors for capped RNA, suggesting that binding of mRNA to hDcp2 is independent of the cap itself (17). A region essential for RNA binding by the hDcp2 protein was demonstrated by deletion mutagenesis. The deleted regi ...
Nutritional Requirements of Streptococcus salivarius
... glutamic acid (Prescott, Ragland & Hurley, 1965). These results indicate that a tricarboxylic acid pathway to a-oxoglutaric acid may be present in streptococci. The primary site of incorporation of ammonia into amino acids in Streptococcus bovis is a nicotinamide adenine dinucleotide phosphate (NADP ...
... glutamic acid (Prescott, Ragland & Hurley, 1965). These results indicate that a tricarboxylic acid pathway to a-oxoglutaric acid may be present in streptococci. The primary site of incorporation of ammonia into amino acids in Streptococcus bovis is a nicotinamide adenine dinucleotide phosphate (NADP ...
Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin
... Splice Forms of Thrombopoietin By Austin L. Gurney, Wun-Jing Kuang, Ming-Hong Xie, Beth E. Malloy, Dan L. Eaton, and Frederic J. de Sauvage Thrombopoietin (TPO), the ligand for c-mpl, is a novel cytokine comprising an amino terminal domain with homology to erythropoietin and a glycosylated carboxyl ...
... Splice Forms of Thrombopoietin By Austin L. Gurney, Wun-Jing Kuang, Ming-Hong Xie, Beth E. Malloy, Dan L. Eaton, and Frederic J. de Sauvage Thrombopoietin (TPO), the ligand for c-mpl, is a novel cytokine comprising an amino terminal domain with homology to erythropoietin and a glycosylated carboxyl ...
Nucleotide sequence of the Tn10 encoded tetracycline resistance
... The finding of a complex, overlapping promotor system for the tetracycline resistance gene carried on Tn10 and described in figure 3 is in agreement with previously published data [2]. A similar arrangement of overlapping promotors has been described for the tet proraotor of pBR322 [25]. The particu ...
... The finding of a complex, overlapping promotor system for the tetracycline resistance gene carried on Tn10 and described in figure 3 is in agreement with previously published data [2]. A similar arrangement of overlapping promotors has been described for the tet proraotor of pBR322 [25]. The particu ...
a guide-book to biochemistry
... having many' growing points', encroaching on the territory of other branches of science-for instance, chemistry, physiology and medicine -and bringing methods of its own to bear' on problems that have not been solved in orthodox ways. It is, perhaps, by this fresh and unconventional approach that th ...
... having many' growing points', encroaching on the territory of other branches of science-for instance, chemistry, physiology and medicine -and bringing methods of its own to bear' on problems that have not been solved in orthodox ways. It is, perhaps, by this fresh and unconventional approach that th ...
Homology Modelling and Structural Comparisons of Capsid
... of MAbs to PCV2 and PCV1. Small mutations, sometimes involving only a single amino acid substitution, were attributed to specific differences in MAb binding between the two porcine viruses [18]. This highlights the selective pressure to employ mutations as a means to elude the host immune system, an ...
... of MAbs to PCV2 and PCV1. Small mutations, sometimes involving only a single amino acid substitution, were attributed to specific differences in MAb binding between the two porcine viruses [18]. This highlights the selective pressure to employ mutations as a means to elude the host immune system, an ...
Cosmetics for Passover 2016
... Due to the fact that lipsticks come into contact with the mouth and perfumes contain alcohol that may be considered as edible, there is more reason to be concerned about chametz derivatives in these products. It has become increasingly difficult to obtain reliable information about these products, s ...
... Due to the fact that lipsticks come into contact with the mouth and perfumes contain alcohol that may be considered as edible, there is more reason to be concerned about chametz derivatives in these products. It has become increasingly difficult to obtain reliable information about these products, s ...
Folic Acid Article from March of Dimes
... The Institute of Medicine also recommends that women eat a diet rich in foods that contain folate or folic acid. Folate is the natural form of folic acid that is found in foods. Orange juice, other citrus fruits and juices, leafy green vegetables, beans, peanuts, broccoli, asparagus, peas, lentils a ...
... The Institute of Medicine also recommends that women eat a diet rich in foods that contain folate or folic acid. Folate is the natural form of folic acid that is found in foods. Orange juice, other citrus fruits and juices, leafy green vegetables, beans, peanuts, broccoli, asparagus, peas, lentils a ...
Isolation of Rat Transferrin Using CNBr-Activated
... Immuno adsorbent columns, loaded with transferrin or antitransferrin, were used over a period of more than two years without loss of activity. ...
... Immuno adsorbent columns, loaded with transferrin or antitransferrin, were used over a period of more than two years without loss of activity. ...
acetyl CoA - LSU School of Medicine
... 6) total energy yield after further metabolism of the acetyl CoA = 131 ATP from one palmitoyl CoA (16:0) (Fig. 16.18). N.B. If you start with the free FA, you need to invest 2 ATP “equivalents” (high energy bonds) in the thiokinase reaction needed to activate the FA. Thus, the net yield of ATP star ...
... 6) total energy yield after further metabolism of the acetyl CoA = 131 ATP from one palmitoyl CoA (16:0) (Fig. 16.18). N.B. If you start with the free FA, you need to invest 2 ATP “equivalents” (high energy bonds) in the thiokinase reaction needed to activate the FA. Thus, the net yield of ATP star ...
Highly Efficient Micro RNA Enrichment
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
Genetic code
The genetic code is the set of rules by which information encoded within genetic material (DNA or mRNA sequences) is translated into proteins by living cells. Biological decoding is accomplished by the ribosome, which links amino acids in an order specified by mRNA, using transfer RNA (tRNA) molecules to carry amino acids and to read the mRNA three nucleotides at a time. The genetic code is highly similar among all organisms and can be expressed in a simple table with 64 entries.The code defines how sequences of these nucleotide triplets, called codons, specify which amino acid will be added next during protein synthesis. With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. Because the vast majority of genes are encoded with exactly the same code (see the RNA codon table), this particular code is often referred to as the canonical or standard genetic code, or simply the genetic code, though in fact some variant codes have evolved. For example, protein synthesis in human mitochondria relies on a genetic code that differs from the standard genetic code.While the genetic code determines the protein sequence for a given coding region, other genomic regions can influence when and where these proteins are produced.