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Evolution of the Actin Gene Family in Testate Lobose Amoebae
Evolution of the Actin Gene Family in Testate Lobose Amoebae

... We estimate that the actin gene family contains 40–50 paralogous members in each lineage. None of the three independent lineages share the same paralog with another, and divergence between actins reaches 29% in contrast to just 2% in SSU-rDNA. Analyses of effective number of codons (ENC), compositio ...
IGF-1 LR3 - Anabolen
IGF-1 LR3 - Anabolen

... IGF-1 stands for insulin-like growth factor. It is a natural substance that is produced in the human body and is at its highest natural levels during puberty. During puberty IGF-1 is the most responsible for the natural muscle growth that occurs during these few years. There are many different thing ...
Introduction to Bioinformatics
Introduction to Bioinformatics

... tRNA • Amino acid attached to each tRNA • Determined by 3 base anticodon sequence (complementary to mRNA) • Translation: process in which the nucleotide sequence of the processed mRNA is used in order to join amino acids together into a protein with the help of ribosomes and tRNA ...
RNAi phenotypes are influenced by the genetic background of the
RNAi phenotypes are influenced by the genetic background of the

... amenable to classical forward genetics. Hence, together with the ease of comprehensively identifying genes by new generation sequencing, RNAi is expanding the scope of animal species and questions that can be addressed in terms of gene function. In the case of genetic mutants, the genetic background ...
(type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli
(type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli

... shown in this paper the genetic determinants of F8 fimbriae were m a p p e d at a position between 17--20 on the chromosome of strain 2980 of E. coli O18:K5. Therefore, it seems that the gene clusters coding for type I fimbriae are located at fixed positions on the chromosomes of different strains. ...
Exon skipping and reading through stop codons
Exon skipping and reading through stop codons

... influence the progress of the disease with drug-like substances. But, with the exception of steroids, all this has not been done in living boys, but in their isolated muscle cells in the laboratory or in animals. However, clinical trials are being planned now for the near future, mainly trials with ...
Endoplasmic Reticulum-Located PDAT1
Endoplasmic Reticulum-Located PDAT1

... In some plants that produce unusual fatty acids, PDAT has been shown to play a major role in removal of these unusual acyl groups from membrane phospholipids (Banas et al. 2000). In the development of castor bean seeds, PDAT activity with high specificity for ricinoleic acid was demonstrated in micr ...
embor2011116-sup-0001
embor2011116-sup-0001

... the naturally occurring amino acid residues. While the algorithm scans a polypeptide sequence, it assigns to each residue i the average aggregation propensity of the residues within a window of a few residues centred on the residue i and generates the aggregation propensity profile (aggregation prop ...


... efficiently with microwave ™ Higher temperatures during deprotection, even at 50ºC (separate experiment) can cleave phosphate groups Æ [-79 mass] ...
The coupling modification of gABA Flyer (, 0.15 kB)
The coupling modification of gABA Flyer (, 0.15 kB)

... molecule and exists as the same form in different species, so the antibodies and kits could be used in the detection of gABA from samples collected from different ...
The 2009 Nobel Prize in Chemistry and KEK`s Photon Factory
The 2009 Nobel Prize in Chemistry and KEK`s Photon Factory

... ribosome crystallography Ribosomes are the cell machinery that produce proteins from amino acids using the information in DNA. This process is called translation. First, genetic information encoded in the DNA located in the nucleus of a cell is copied onto a piece of messenger RNA (mRNA). Next, the ...
chapter2 review of literature
chapter2 review of literature

... world average productivity of 467 kg/ha (Fig 3). India had been the dominant producer till 2005 accounting for almost 25 per cent of the world output. However, it lost to China in 2006 followed by Myanmar from 2007 (Fig 4). This decreasing crop yield can be attributed to its cultivation in un-irriga ...
The p101 subunit of PI3Kγ restores activation by Gβ mutants
The p101 subunit of PI3Kγ restores activation by Gβ mutants

... Class I PI3Ks (phosphoinositide 3-kinases) are secondmessenger-generating enzymes, which transform extracellular signals into the principle product PtdIns(3,4,5)P3 in order to control a plethora of fundamental cellular responses, including proliferation, differentiation, growth and chemotaxis [1–8]. ...
Chapter 1 - Institut Montefiore
Chapter 1 - Institut Montefiore

... • Transfer RNA (tRNA) molecules transport amino acids to the growing protein chain. Each tRNA carries an amino acid at one end and a threebase pair region, called the anticodon, at the other end. The anticodon bonds with the codon on the protein chain via base pair matching. ...


... procedures to produce lownonspecific-binding avidins suitable for specialized applications, e.g., avidin-D and avidin-DN (Vector Laboratories, Burlingame, CA). The biotin-binding protein streptavidin also consists of four subunits but contains no carbohydrate; its p1 is 5-6. Streptavidin is widely ...
Novel FAD3 Mutant Allele Combinations Produce Soybeans
Novel FAD3 Mutant Allele Combinations Produce Soybeans

... 2000). Other soybean germplasm is available with approximately 3% linolenic acid, and those lines have been shown to contain two genes conditioning the trait (Bilyeu et al., 2005; Fehr et al., 1992; Stojsin et al., 1998; Takagi et al., 1999). Independent work on the molecular genetic basis for the l ...
BIO315109 Assessment Report
BIO315109 Assessment Report

... work its way into the field influencing nutrient levels’ or ‘cattle may get through the fence and decimate crops’. General terms such as weather, climate and the environment were not accepted. This was a difficult question for a number of candidates. So many variables needed to be controlled and the ...
Higher order structural effects stabilizing the
Higher order structural effects stabilizing the

... to mediate the interaction between the D-loop and the Vloop in tRNA structures, seems not to be intrinsically stable from a geometrical point of view (29). Instead of a G:C W:W trans, the gas phase optimized G15–C48 geometry is a bifurcated one, involving the central section of the G Watson–Crick fa ...
Laroche: Mouse Colouration
Laroche: Mouse Colouration

... Amino Acids 230 → 234 3’–GAACAGGTGGTGCCA–5’ ...
History and Philosophy of Science
History and Philosophy of Science

... To score the similarity of two words, BLAST builds a table based on edit distances. For example, comparing AGCT to ACCC could give a score of 1, whereas comparing it to GGCT would give 3. However, some substitutions (due to mutation) are more likely than others, especially in the case of amino acids ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
Promega Notes 49: Firefly Luciferase Engineered for Improved

... sequences for transcription factor binding sites (5) and removed many sites which could potentially interact with common factors. In some cases where it was convenient in our modification strategy, we also removed less common potential regulatory sites. As with the removal of restriction sites, the ...
Microbial Cause of Calcium Lactate Defect in Cheddar Cheese
Microbial Cause of Calcium Lactate Defect in Cheddar Cheese

... Type of packaging showed a significant difference on control cheese, however, no difference was observed with the A/A+ cheeses. ...
trace minerals in poultry nutrition
trace minerals in poultry nutrition

... manufacturers, in an attempt to bring the cost lower, use cheaper and locally available ingredients; hence the supplies show enough of compositional variability. Lall and Prasad analyzed samples of mineral mixtures marketed by 10 different firms and found that none of them matched the BIS specificat ...
Staphylococcus haemolyticus lipase
Staphylococcus haemolyticus lipase

... Lipase activity for p-nitrophenyl esters was measured spectrophotometrically [12,13]. One milliliter of p-nitrophenyl esters (C2 ^C12 , 10 mM in acetonitrile) was mixed with ethanol (4 ml) and 95 ml of 50 mM Tris-HCl (pH 8.5). An appropriate amount of the lipase was added to 1 ml of this freshly pre ...
oculocutaneous albinism and analysis of tyrosinase gene in
oculocutaneous albinism and analysis of tyrosinase gene in

... All of the homozygous cases were unrelated, and their family histories indicated no consanguineous marriages. But in each homozygous case, the parent's native place was the same city or prefecture. Therefore, we postulated that the people in Japan have inhabited an area without moving for many gener ...
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Expanded genetic code



An expanded genetic code is an artificially modified genetic code in which one or more specific codons have been re-allocated to encode an amino acid that is not among the 22 encoded proteinogenic amino acids.The key prerequisites to expand the genetic code are: the non-standard amino acid to encode, an unused codon to adopt, a tRNA that recognises this codon, and a tRNA synthase that recognises only that tRNA and only the non-standard amino acid.Expanding the genetic code is an area of research of synthetic biology, an applied biological discipline whose goal is to engineer living systems for useful purposes. The genetic code expansion enriches the repertoire of useful tools available to science.
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