wt - EMBO
... centrosomes can assemble microtubules in cnn1-/- cells in vivo many unanswered questions....are centrosomes intact otherwise in cells? how does centrosome detachment arise? how dynamic is centrosome detachment? ...
... centrosomes can assemble microtubules in cnn1-/- cells in vivo many unanswered questions....are centrosomes intact otherwise in cells? how does centrosome detachment arise? how dynamic is centrosome detachment? ...
File - HABITAT (Home)
... Also called a chemical bond. A connection between two atoms either as a result of sharing (covalent bond) or transferring (ionic ...
... Also called a chemical bond. A connection between two atoms either as a result of sharing (covalent bond) or transferring (ionic ...
New Research Evaluating Cisplatin Uptake in Ovarian Cancer Cells
... starvation had no effect on the uptake of cisplatin in either the A2780 or the A2780/CP70 cell lines; both show similar cisplatin uptake rates as the control cells. Thus, these results suggest that the heterogeneous distribution of cisplatin uptake in cells is not due to the cell cycle but rather so ...
... starvation had no effect on the uptake of cisplatin in either the A2780 or the A2780/CP70 cell lines; both show similar cisplatin uptake rates as the control cells. Thus, these results suggest that the heterogeneous distribution of cisplatin uptake in cells is not due to the cell cycle but rather so ...
AP Biology Cell Transport and Osmoregulation Multiple Choice
... 32. The transfer of fluid from the glomerulus to Bowman's capsule A) results from active transport. B) transfers large molecules as easily as small ones. C) is very selective as to which small molecules are transferred. D) is mainly a consequence of blood pressure force-filtering the fluid. E) usua ...
... 32. The transfer of fluid from the glomerulus to Bowman's capsule A) results from active transport. B) transfers large molecules as easily as small ones. C) is very selective as to which small molecules are transferred. D) is mainly a consequence of blood pressure force-filtering the fluid. E) usua ...
Chapter 5 MICROSCOPY: IMAGING OF BIOLOGICAL SPECIMENS
... also permeabilizes the cell. Finally, in some (rare) cases, permeabilization without fixation can be accomplished with pore-forming agents such as streptolysin-O (SLO). SLO is a toxin that comes from Streptococcus aureus. It binds cholesterol in membranes and aggregates, forming pores. SLO is used m ...
... also permeabilizes the cell. Finally, in some (rare) cases, permeabilization without fixation can be accomplished with pore-forming agents such as streptolysin-O (SLO). SLO is a toxin that comes from Streptococcus aureus. It binds cholesterol in membranes and aggregates, forming pores. SLO is used m ...
CHAPTER 4 FREE ENERGY AND CHEMICAL EQUILIBRIA
... To attain equilibrium, some of the NaCl will move. It will move from the outer solution through the membrane into the bag with the protein in it. In effect, the added salt reduces and at high enough salt concentration, eliminates the Donnan effect. ...
... To attain equilibrium, some of the NaCl will move. It will move from the outer solution through the membrane into the bag with the protein in it. In effect, the added salt reduces and at high enough salt concentration, eliminates the Donnan effect. ...
chapter 3 9 - GeneralBiology-SN
... Target cells possess the specific protein receptor to which the hormone must bind; 2. Transduction Amplification of the stimulus and its conversion into a second messenger Calcium ions appear to be important second messengers in many plant responses. Calcium ions bind to the protein calmoduli ...
... Target cells possess the specific protein receptor to which the hormone must bind; 2. Transduction Amplification of the stimulus and its conversion into a second messenger Calcium ions appear to be important second messengers in many plant responses. Calcium ions bind to the protein calmoduli ...
How Do You Clone a Gene?
... proteins to work properly, they must have the correct conformation, which is unique to each protein. If a protein does not have the proper conformation, its biological activity may be decreased or totally lost. EDVO-Links™ can also be used to simulate proteins, where the links represent one or more ...
... proteins to work properly, they must have the correct conformation, which is unique to each protein. If a protein does not have the proper conformation, its biological activity may be decreased or totally lost. EDVO-Links™ can also be used to simulate proteins, where the links represent one or more ...
Mimic in vivo-like Flow Conditions and Achieve Reliable
... and quantifying morphology changes in the sub-nanometer to micrometer range. In ECIS, a small alternating current (I) is applied across an electrode pattern at the bottom of an array. This results in a potential (V) across the electrodes that is measured by the ECIS instrument. The impedance (Z) is ...
... and quantifying morphology changes in the sub-nanometer to micrometer range. In ECIS, a small alternating current (I) is applied across an electrode pattern at the bottom of an array. This results in a potential (V) across the electrodes that is measured by the ECIS instrument. The impedance (Z) is ...
Chemical organization of cells. Macromolecules
... repeats – a region of dyad symmetry (fig. 16). In a double-strand DNA, the complementary sequences on one strand have the opportunity to base pair only if the strand separates from its partner. As a result a hairpin could be formed. The formation of two apposed hairpins creates a cruciform. Palindro ...
... repeats – a region of dyad symmetry (fig. 16). In a double-strand DNA, the complementary sequences on one strand have the opportunity to base pair only if the strand separates from its partner. As a result a hairpin could be formed. The formation of two apposed hairpins creates a cruciform. Palindro ...
[Click here and type Date]
... capable of supporting growth of blood cells in vitro. Stromal cells that make the matrix within the bone marrow are also derived from mesenchymal stem cells. Subculturing – Transferring cultured cells, with or without dilution, from one culture vessel to another. Surface markers – Proteins on the ou ...
... capable of supporting growth of blood cells in vitro. Stromal cells that make the matrix within the bone marrow are also derived from mesenchymal stem cells. Subculturing – Transferring cultured cells, with or without dilution, from one culture vessel to another. Surface markers – Proteins on the ou ...
ImmunoBioScience Corp. (IBSC) DATA SHEET Bromodeoxyuridine
... Description: Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside which is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase ...
... Description: Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside which is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase ...
Chapter 32 - s3.amazonaws.com
... that is coded for by the codon, therefore that amino acid gets localized to the ribosome • If they collect the ribosomes from the experiment they can identify which amino acid was brought to the ribosome by that triplet codon ...
... that is coded for by the codon, therefore that amino acid gets localized to the ribosome • If they collect the ribosomes from the experiment they can identify which amino acid was brought to the ribosome by that triplet codon ...
protein - mustafaaltinisik.org.uk
... Most of the 20 common amino acids found in proteins were discovered. 1864 Hoppe-Seyler crystallized, and named, the protein hemoglobin. 1894 Fischer proposed a lock-and-key analogy for enzyme-substrate interactions. 1897 Buchner and Buchner showed that cell-free extracts of yeast can ferment sucrose ...
... Most of the 20 common amino acids found in proteins were discovered. 1864 Hoppe-Seyler crystallized, and named, the protein hemoglobin. 1894 Fischer proposed a lock-and-key analogy for enzyme-substrate interactions. 1897 Buchner and Buchner showed that cell-free extracts of yeast can ferment sucrose ...
PATHOGENESIS OF GLOMERULAR INJURY
... in the systemic circulation. Autoantibodies against neutrophil cytoplasmic antigens in the circulation may represent an additional mechanism of antibodymediated glomerular injury in patients without discernible immune complexes in the glomerular parenchyma ...
... in the systemic circulation. Autoantibodies against neutrophil cytoplasmic antigens in the circulation may represent an additional mechanism of antibodymediated glomerular injury in patients without discernible immune complexes in the glomerular parenchyma ...
- bzh - Uni Heidelberg
... coupled with en bloc transfer from its import adaptor Syo1 to 5S rRNA could ensure a coordinated and stoichiometric assembly of the 5S RNP onto the evolving pre-60S subunit. Despite the advances in our compositional understanding of pre-ribosomal particles, only little structural data is available f ...
... coupled with en bloc transfer from its import adaptor Syo1 to 5S rRNA could ensure a coordinated and stoichiometric assembly of the 5S RNP onto the evolving pre-60S subunit. Despite the advances in our compositional understanding of pre-ribosomal particles, only little structural data is available f ...
protein synthesis
... delivered into stroma and then directed by internal targeting signal into thylakoid membrane ...
... delivered into stroma and then directed by internal targeting signal into thylakoid membrane ...
Purification of DNA from cultured animal cells using the
... 19. Centrifuge at 6000 rpm (~5790 x g) for 15 min. The heat generated during centrifugation ensures evaporation of residual ethanol in the sample (from Buffer AW2) that might otherwise inhibit downstream reactions. 20. Place the DNeasy 96 plate on top of a rack of elution microtubes (provided). 21. ...
... 19. Centrifuge at 6000 rpm (~5790 x g) for 15 min. The heat generated during centrifugation ensures evaporation of residual ethanol in the sample (from Buffer AW2) that might otherwise inhibit downstream reactions. 20. Place the DNeasy 96 plate on top of a rack of elution microtubes (provided). 21. ...
Instructor`s Guide
... phospholipid molecule: A molecule that consists of a water-soluble, hydrophilic head and a nonwater soluble, hydrophobic tail. These molecules align themselves so that their heads face toward the inner and outer surfaces of the cell, while the tails line up in the center of the membrane. plasma memb ...
... phospholipid molecule: A molecule that consists of a water-soluble, hydrophilic head and a nonwater soluble, hydrophobic tail. These molecules align themselves so that their heads face toward the inner and outer surfaces of the cell, while the tails line up in the center of the membrane. plasma memb ...
Inhibitors
... IC 50 for other related molecules Use lowest dose possible Verify it does not affect other pathways ...
... IC 50 for other related molecules Use lowest dose possible Verify it does not affect other pathways ...
Biology formula and tips
... either asexual or with the help of fission or budding. (Sexual reproduction, mitosis and meiosis are absent) ...
... either asexual or with the help of fission or budding. (Sexual reproduction, mitosis and meiosis are absent) ...
DNA - The Double Helix
... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
DNA - The Double Helix
... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.