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COS 3.0 Acids and Bases
COS 3.0 Acids and Bases

... slippery in water solution, turn red litmus to blue, and react with acids to form salts. (measures more than 7 on the pH scale) • NEUTRAL - These are items that are neither acids or bases. There are 4 main ways to determine if a substance is and acid or a base. They are: Red litmus paper, Blue litmu ...
function finders
function finders

... -- Each P53 molecule has three regions: one binds to other P53 molecules, one switches genes on and one binds to DNA. -- P53 has several anti-cancer properties: it can activate DNA repair proteins when DNA has been damaged; it can suspend cell division, allowing time for damaged DNA to be repaired; ...
ppt
ppt

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explanation - mbhsbiologystaar
explanation - mbhsbiologystaar

... • The base-pairing rules, based on Chargoff’s research, are • A-T, G-C ...
344-352
344-352

... Although conventional hydrogen bonds that involve electronegative atoms like oxygen and nitrogen have been thoroughly studied over the decades since their first introduction into the literature and are presently well understood [19-21], but the CH…O interaction is thought to be crucial in a large of ...
PPT - Altogen Biosystems
PPT - Altogen Biosystems

... Altogen Biosystems offers the HCAEC Cell Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HCAEC Cell Transfection Reagent is a nanoparticle-based liposome mediated formulation, and it has been developed to provide high transfection efficiency with HCAEC. T ...
Download PDF
Download PDF

... sequencing-based techniques for quantifying strain abundance;17 and microfluidic technology for high-specificity cell sorting based on differences in the intrinsic electrical properties of individual cells.18 By sorting the deletion collection into fractions with different electrical characteristics ...
Lecture 4 POWERPOINT here
Lecture 4 POWERPOINT here

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... The stability of protein solutions is governed not only by the macromolecular net charge, salt concentration and valency, but also on the chemical nature of the dissolved ions (1). Traditionally, the latter falls under the category of Hofmeister or ion-specific effects which in recent years has seen ...
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Ch 20 Notes - Dublin City Schools
Ch 20 Notes - Dublin City Schools

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Chapter 3 Study Guide
Chapter 3 Study Guide

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CH 17 PPT

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... hydroperoxide and other similar compounds allow rapid conversion of PEI capped gold cations into AuNPs at room temperature thus controlling the functional ability of nanoparticles as a function of organic reducing agents. Both small and higher molecular weight PEI facilitates fast synthesis of AuNPs ...
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AMINOACETYLATION OF t-RNA

... The specific linkage of the correct amino acid to each tRNA is accomplished by aminoacyl-tRNA synthetases. Due to the degeneracy of the genetic code, some of the different tRNAs have the same amino acid attached to them. Aminoacyl-tRNA (also known as charged tRNA) is produced in two steps; amino ac ...
Protein Synthesis
Protein Synthesis

... comparison of the structures of procaryotic and eucaryotic ribosomes. Ribosomal components are commonly designated by their “S values,” which refer to their rate of sedimentation in an ultracentrifuge. Despite the differences in the number and size of their rRNA and protein components, both procary ...
Antibody to bcl-2 Oncoprotein
Antibody to bcl-2 Oncoprotein

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Chaperone Competent Cell BL21
Chaperone Competent Cell BL21

... E.coli is commonly used as a host for protein expression, as it provides the simplest system to use and wide choice in expression systems. However, expression of foreign proteins in E.coli often results in various problems with the expressed proteins such as formation of inclusion bodies and degrada ...
Michael S. Chimenti PhD “Michael has been the linchpin in our
Michael S. Chimenti PhD “Michael has been the linchpin in our

... Storm for Cancer Progression. Nature Reviews Cancer 11: 671-677. Baran KL, Chimenti MS, Schlessman JL, Fitch CA, Herbst KJ, and Garcia-Moreno B (2008). Electrostatic effects in a network of polar and ionizable groups in staphylococcal nuclease. Journal of Molecular Biology 379: 1045-1062. Harms MJ, ...
DMA Damage as a Basis for 4
DMA Damage as a Basis for 4

... were made using the alkaline elution technique as opposed to the alkaline sucrose gradient sedimentation method used by the previous investigators. We have also extended their find ings by demonstrating 2 additional forms of DNA damage. Both DNA DSBs and DNA-protein cross-links occur in drug-treated ...
File - Mizzou Pre
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An Honors Thesis Proposal
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... the presence of HPV16 genes using a reporter gene assay. A reporter gene assay utilizes specialized cloned DNA plasmids. These plasmids contain the gene for an easily measurable protein, in this case firefly luciferase, the protein which under certain chemical conditions causes fireflies to glow. A ...
FDG PET imaging
FDG PET imaging

... Gallium sensitivity is reported to be better than 85% for high grade tumors. Sensitivity for low grade (slow growing) tumors is poor. A persistently positive Ga-67 exam after one cycle of treatment or at midtreatment for nonHodgkins lymphoma is associated with a higher likelihood for treatment failu ...
Bacteriophage
Bacteriophage

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Cell-penetrating peptide



Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.
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