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If you have a the following genotypes as babies, what must the
If you have a the following genotypes as babies, what must the

... • Transcription happens first (how / where?) • Translation happens second (how / where?) • What is a codon? - a three nucleotide sequence that codes for a specific amino acid. ...
a5_1_1-1_done
a5_1_1-1_done

Recombinant DNA - Westwind Alternate School
Recombinant DNA - Westwind Alternate School

... joining together gene of interest within plasmid, producing recombinant DNA 9. State two examples of the current uses of genetically modified crops or animals genetically modified cows contain human genes for making medically important proteins (insulin, growth hormone) human proteins are extracted ...
dna methylation
dna methylation

... Calorie consumption dropped from 2,000 to 500 per day for 4.5 million. Children born or raised in this time were small, short in stature and had many diseases including, edema, anemia, diabetes and depression. The Dutch Famine Birth Cohort study showed that women living during this time had children ...
dna methylation
dna methylation

... Calorie consumption dropped from 2,000 to 500 per day for 4.5 million. Children born or raised in this time were small, short in stature and had many diseases including, edema, anemia, diabetes and depression. The Dutch Famine Birth Cohort study showed that women living during this time had children ...
Document
Document

... DNA ligase covalently links ...
Classical and Modern Genetics
Classical and Modern Genetics

... • Fidelity in copying information • Specificity in information • Expression of gene via manufacturing of polypeptide leading to protein (e.g., enzyme) • Genetic Code is conserved in evolution – all organisms use the exact same coding process • Example of Genetic Code: laboratory exercise ...
Provincial Exam Questions
Provincial Exam Questions

... 7. If 20% of the base molecules are guanine, how many thymine molecules are present in a DNA molecule with 1000 bases? A. 200 B. 300 C. 400 D. 600 ...
Gene Cloning And DNA vs - Mr. Lesiuk
Gene Cloning And DNA vs - Mr. Lesiuk

... that desired gene (DNA). The DNA must be pre-processed into (cDNA) "Complimentary DNA" before it was actually joined to the vector. - Remember that during transcription DNA mRNA, but before the mRNA can be translated it must have specific Ribozymes (NOT Ribosomes) cut out the useless introns and sav ...
DNA Mutation and Repair
DNA Mutation and Repair

... A base analogue is a substance other than a standard nucleic acid base that can be incorporated into a DNA molecule by the normal process of polymerization. Such a substance must be able to pair with the base on the complementary strand being copies, or the 3'->5' editing function will remove it. Fo ...
Option D Evolution - A - Origin of Life
Option D Evolution - A - Origin of Life

... II. Organic molecules to Protobionts Protobionts - aggregate of abiotically produced organic molecules surrounded by a membrane or a membrane-like structure. ii. The assembly of simple organic molecules into polymers iii. The origin of self-replication molecules that made inheritance possible i. “Ge ...
Protein Synthesis - Elgin High School
Protein Synthesis - Elgin High School

... it did for replication – mRNA (messenger RNA) links to the nucleotides link to the open strand, making a complimentary copy of the DNA. • Always read from the 5’ toward the 3’ end of the DNA strand. • The DNA strand is read in a series of 3, called triplets. • The complimentary mRNA strand is called ...
Zipf*s monkeys
Zipf*s monkeys

...  A gene is copied (transcription) off the genome, and ...
Chapter 24
Chapter 24

... 3. RNase H degrades the viral RNA. 4. The second DNA strand is synthesized on the first DNA strand. • RT does not have the editing function (3’→5’ exonuclease). Therefore there is high error rate in replication, and thus RT has a high mutation rate. • AZT, ddI, ddC, and 2’3’-didehydro-3’-deoxythymin ...
CSI” Plant Style: From Laboratory to your Lunch Tray
CSI” Plant Style: From Laboratory to your Lunch Tray

... Gene—a sequence of DNA that occupies a specific location and determines a ...
dna replication
dna replication

... DNA Pol… …more facts • Has a proofreading mechanism built in • Checks for base matching • Removes mismatched bases by going backwards • Reason why it is not able to build DNA in the 3’ to 5’ direction. No energy from ATP hydrolysis. • Makes just one error in 10E8 or 10E9 (billion) bases added ...
Special Study Project III
Special Study Project III

... c. the complete separation of the original strands, the synthesis of new strands and the reassembly of double-stranded molecules. d. the use of the original double-stranded molecule as a template, without unwinding. e. none of the above. ...
Chapter 23 (Part 1)
Chapter 23 (Part 1)

... Eli Lilly ...
Chapter 2
Chapter 2

... Ribonucleic acid (RNA) is quite similar to DNA. There are three major differences: 1. In RNA, there is no thymine (T) which exists in DNA. Instead, uracil (U) is found in RNA. In other words, RNA is constructed out of A, G, C and U. 2. DNA has a double helix structure while RNA has only one strand. ...
Protein Synthesis Simulation Lab
Protein Synthesis Simulation Lab

... Another peculiar thing about DNA is that it is located inside the nucleus, and pretty much stays inside the nucleus, yet the proteins that DNA helps to make are produced OUTSIDE of the nucleus. So how does the cell solve this problem? It sends a “messenger” from the nucleus to the ribosomes in the c ...
Biology 1 Notes Chapter 12 - DNA and RNA Prentice Hall
Biology 1 Notes Chapter 12 - DNA and RNA Prentice Hall

... 1) Transcription (occurs in the ...
population_genetics_and_human_evolution_final2
population_genetics_and_human_evolution_final2

... DNA is then extracted from the cell nucleus by addition of chemicals which break the cells open. The DNA is then isolated from other components of the cell DNA Copying Since only small amount of DNA is extracted for forensic analysis, the short tandem repeats in every genetic locus are amplified usi ...
Restriction fragment length polymorphism
Restriction fragment length polymorphism

... • The polymerase chain reaction is an extremely versatile technique for copying DNA. • PCR allows a single DNA sequence to be copied (millions of times), or altered in predetermined ways. • PCR has many variations, like reverse transcription PCR (RT-PCR) for amplification of RNA, and real-time PCR ( ...
Agarose Gel Electrophoresis Description An electrophoresis
Agarose Gel Electrophoresis Description An electrophoresis

... recovery of DNA. Lower voltages, coupled with longer running times, provide optimum resolution, such as that required for Southern Blots or forensic applications. Pulsed-field electrophoresis can be used to separate very large DNA fragments. The most common stain is ethidium bromide, which intercala ...
Document
Document

... Sequence of DNA bases on a chromosome that determines the amino acid sequence of a protein ...
< 1 ... 814 815 816 817 818 819 820 821 822 ... 1026 >

Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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