dna TRANSCRIPTION AND tRANSLATION
... Pyrimidines: Thymine, Cytosine, and Uracil (note: in RNA, Uracil replaces Thymine) Nucleotide pairing – Also known as base pairing, is the joining of purine to a pyrimidine through a hydrogen bond link (A—T, C—G, or A—U in RNA) Ribonucleic acid (RNA) – Messenger to carry instructions from DNA for co ...
... Pyrimidines: Thymine, Cytosine, and Uracil (note: in RNA, Uracil replaces Thymine) Nucleotide pairing – Also known as base pairing, is the joining of purine to a pyrimidine through a hydrogen bond link (A—T, C—G, or A—U in RNA) Ribonucleic acid (RNA) – Messenger to carry instructions from DNA for co ...
Bacterial Transformation with (pGLO Plasmid)
... Sugar source for energy & carbon Process that increases permeability of the cell membrane to DNA Green Fluorescent Protein (w/UV) ...
... Sugar source for energy & carbon Process that increases permeability of the cell membrane to DNA Green Fluorescent Protein (w/UV) ...
palm-print on stickers as a replacement of blood
... From time to time for unknown reasons, the DNA obtained by the above procedure was not clean enough to give reliable DNA profiles. We found that spin-column chromatography using Sephadex G-50 (Sigma) or comparable measures like Microcon (Amicon) ultrafiltration seemed to be helpful (Figure 5). The ...
... From time to time for unknown reasons, the DNA obtained by the above procedure was not clean enough to give reliable DNA profiles. We found that spin-column chromatography using Sephadex G-50 (Sigma) or comparable measures like Microcon (Amicon) ultrafiltration seemed to be helpful (Figure 5). The ...
Enzyme Kinetics
... • Proximity/orientation effect • Transition State Stabilization • Electrostatic catalysis ...
... • Proximity/orientation effect • Transition State Stabilization • Electrostatic catalysis ...
Minos, a new transposable element from Drosophila hydei, is a
... sequence (2) (Figure 1). Southern blots of restricted DNA from two D. hydei strains showed distinct banding patterns (Figure 1), suggesting that the element is, or has until recently been mobile. The element was named Minos, after the legendary king who inhabited the palace located near our laborato ...
... sequence (2) (Figure 1). Southern blots of restricted DNA from two D. hydei strains showed distinct banding patterns (Figure 1), suggesting that the element is, or has until recently been mobile. The element was named Minos, after the legendary king who inhabited the palace located near our laborato ...
Zinc finger nucleases
... exogenously introduced homologous DNA fragment, also referred to as the "donor DNA".In the absenceofaDSBat the targetlocus, typically fewer than1in105 of targetedcellswillcontain the desired genetic modification, a frequency too low to be useful for gene therapy[14]. However, proof‐of‐principle expe ...
... exogenously introduced homologous DNA fragment, also referred to as the "donor DNA".In the absenceofaDSBat the targetlocus, typically fewer than1in105 of targetedcellswillcontain the desired genetic modification, a frequency too low to be useful for gene therapy[14]. However, proof‐of‐principle expe ...
Quantification of nucleic acids
... maxima of the DNA-YO-PRO-1 complex are 491 and 509, respectively. The sensitivity is over 400-fold greater than that of Hoechst 33258. The fluorochrome binds to RNA as well as DNA. The RNA and DNA content in a sample may be quantified by measuring the fluorescence before and after treatment with DNasef ...
... maxima of the DNA-YO-PRO-1 complex are 491 and 509, respectively. The sensitivity is over 400-fold greater than that of Hoechst 33258. The fluorochrome binds to RNA as well as DNA. The RNA and DNA content in a sample may be quantified by measuring the fluorescence before and after treatment with DNasef ...
The Unseen Genome
... For some reason, makorin1--and apparently only makorin1 all but shuts down when its pseudogene pl is knocked out. RNA made from the pseudogene, in other words, controls the expression of the "real" gene whose sequence it mimics, even though the two lie on different chromosomes. There is nothing pseu ...
... For some reason, makorin1--and apparently only makorin1 all but shuts down when its pseudogene pl is knocked out. RNA made from the pseudogene, in other words, controls the expression of the "real" gene whose sequence it mimics, even though the two lie on different chromosomes. There is nothing pseu ...
UNIT 1: DNA and the Genome
... keratin (hair); the cells found in heart tissue do not produce any digestive enzymes like pepsin or amylase, as they are not required. ...
... keratin (hair); the cells found in heart tissue do not produce any digestive enzymes like pepsin or amylase, as they are not required. ...
Adapted
... 1. Plant wound phenolics sense by VirA signal passed to VirG T-DNA excise 2. Phenolics plant wound sense by VirA signal passed to VirG T-DNA excise 3. Plant wound phenolics sense by VirG signal passed to VirA T-DNA excise 4. Plant wound Signal passed to VirG phenolics sense ...
... 1. Plant wound phenolics sense by VirA signal passed to VirG T-DNA excise 2. Phenolics plant wound sense by VirA signal passed to VirG T-DNA excise 3. Plant wound phenolics sense by VirG signal passed to VirA T-DNA excise 4. Plant wound Signal passed to VirG phenolics sense ...
02-3 Carbon Compounds
... 1. Primary Structure (single strand) 2. Secondary Structure (alpha helix or pleated sheet) 3. Tertiary Structure (folded helixes, sheets) 4. Quartenary Structure (2 or more polypeptides together) ...
... 1. Primary Structure (single strand) 2. Secondary Structure (alpha helix or pleated sheet) 3. Tertiary Structure (folded helixes, sheets) 4. Quartenary Structure (2 or more polypeptides together) ...
Understanding DNA / Chromatin / Chromosomes
... Step 6) What do the terms diploid and haploid mean? What do the symbols n or 2n mean? Make sure you know this in class. You do not need to write anything into your packet unless you want to. For the next two questions, here is a reminder of how to draw DNA structures within a nucleus: Focus on the n ...
... Step 6) What do the terms diploid and haploid mean? What do the symbols n or 2n mean? Make sure you know this in class. You do not need to write anything into your packet unless you want to. For the next two questions, here is a reminder of how to draw DNA structures within a nucleus: Focus on the n ...
Non-adaptive Evolution Natural Selection Natural Selection
... – Movement into or out of a population – Movement into is only gene flow if immigrant successfully breeds if immigrant successfully breeds – More likely than drift to be beneficial – However, possibility of outbreeding d ...
... – Movement into or out of a population – Movement into is only gene flow if immigrant successfully breeds if immigrant successfully breeds – More likely than drift to be beneficial – However, possibility of outbreeding d ...
draft key
... A and C must be 30 map units (cM) apart. In a cross, they exhibit only 26% recombination due to the effect of multiple crossovers. ...
... A and C must be 30 map units (cM) apart. In a cross, they exhibit only 26% recombination due to the effect of multiple crossovers. ...
RNA Synthesis and Splicing
... Modification: 1. Cleavage of primary transcript by Ribonuclease III 2. Modification of bases (Prokaryotes: methylation) and ribose (Eukaryotes: methylation) ...
... Modification: 1. Cleavage of primary transcript by Ribonuclease III 2. Modification of bases (Prokaryotes: methylation) and ribose (Eukaryotes: methylation) ...
Chapter 5 - FIU Faculty Websites
... double stranded DNA with NaOH. 2) DNA primers (short pieces of DNA that are both complementary to the strand which is to be sequenced and radioactively labelled at the 5' end) 3) a mixture of a particular ddNTP (1%) 4) Other four dNTPs (dCTP, dGTP, and dTTP). The concentration of ddATP should be 1% ...
... double stranded DNA with NaOH. 2) DNA primers (short pieces of DNA that are both complementary to the strand which is to be sequenced and radioactively labelled at the 5' end) 3) a mixture of a particular ddNTP (1%) 4) Other four dNTPs (dCTP, dGTP, and dTTP). The concentration of ddATP should be 1% ...
Unit 4: Viruses Intro Video Anatomy of a Virus
... • Because viruses depend on cells for their own propagation, it is reasonable to assume that they evolved after the first cells appeared. • Most molecular biologists favor the hypothesis that viruses originated from fragments of cellular nucleic acids that could move from one cell to another. ...
... • Because viruses depend on cells for their own propagation, it is reasonable to assume that they evolved after the first cells appeared. • Most molecular biologists favor the hypothesis that viruses originated from fragments of cellular nucleic acids that could move from one cell to another. ...
The Human Genome Project - Homepages | The University of
... • A panel of 100-200 hybrids with 5-10 different fragments of human DNA in each gives about 1000 fragments in total, i.e. the human genome has been divided into 1000 bits. • The closer together 2 markers are in the genome, the more likely it is that they will be present in the same hybrids (since th ...
... • A panel of 100-200 hybrids with 5-10 different fragments of human DNA in each gives about 1000 fragments in total, i.e. the human genome has been divided into 1000 bits. • The closer together 2 markers are in the genome, the more likely it is that they will be present in the same hybrids (since th ...
DNA Extraction from Plant and Animal Cells
... treated with cellulase than those treated without. This is due to the action of the enzyme cellulase in breaking down the cellulose of plant cell walls. The amount of DNA extracted from animal cells depends on the type of animal tissue being used. The shrimp sample probably resulted in less DNA than ...
... treated with cellulase than those treated without. This is due to the action of the enzyme cellulase in breaking down the cellulose of plant cell walls. The amount of DNA extracted from animal cells depends on the type of animal tissue being used. The shrimp sample probably resulted in less DNA than ...
Where is DNA in a euk cell?
... Protein synthesis: Adding amino acids & forming peptide bonds Which level of protein structure is being assembled? A. primary B. secondary C. tertiary D. quaternary Microtubules and Microfilaments What do they have in common? A. components of the cytoskeleton B. made of tubulin C. only found in plan ...
... Protein synthesis: Adding amino acids & forming peptide bonds Which level of protein structure is being assembled? A. primary B. secondary C. tertiary D. quaternary Microtubules and Microfilaments What do they have in common? A. components of the cytoskeleton B. made of tubulin C. only found in plan ...
Biochemistry I INTRO
... General information in E. coli DNA Calculate the length of an E. coli DNA molecule. Compare the length of the DNA molecule with the cell dimensions. How does the DNA molecule fit into the cell? Assume that the average protein in E. coli consist of a chain of 400 amino acids. What is the maximum numb ...
... General information in E. coli DNA Calculate the length of an E. coli DNA molecule. Compare the length of the DNA molecule with the cell dimensions. How does the DNA molecule fit into the cell? Assume that the average protein in E. coli consist of a chain of 400 amino acids. What is the maximum numb ...
Calling names
... translated into amino acid sequences • The “words” of the DNA “language” are triplets of bases called codons – 3 bases or nucleotides make one codon – Each codon specifies an amino acid – The codons in a gene specify the amino acid sequence of a polypeptide ...
... translated into amino acid sequences • The “words” of the DNA “language” are triplets of bases called codons – 3 bases or nucleotides make one codon – Each codon specifies an amino acid – The codons in a gene specify the amino acid sequence of a polypeptide ...
C b
... Very often in biochemical experiments it is used complicate buffer systems consisting of some organical components (TRIS, EDTA), two carged metalloions, so on; At interaction of many biopolymers with some dyes, drugs, metal ions heving more than one ionic charge aggregation, sedimentation of biomacr ...
... Very often in biochemical experiments it is used complicate buffer systems consisting of some organical components (TRIS, EDTA), two carged metalloions, so on; At interaction of many biopolymers with some dyes, drugs, metal ions heving more than one ionic charge aggregation, sedimentation of biomacr ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.