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Gene rearrangements occur via various mechanisms
Gene rearrangements occur via various mechanisms

... chromosome being changed); or by the breaking and rejoining of DNA strands, which forms new molecules of DNA. Crossing-over (homologous recombination) is one such mechanism by which DNA variations can occur, and genes can be rearranged. Crossing-over is an event that occurs during meiosis when chrom ...
1. What is the collective term for all of the chemical processes
1. What is the collective term for all of the chemical processes

... Simple _____ epithelial cells are specialized for secretion and are found in glands such as the pancreas. A) ...
BIOL 1107 - Chapter 17
BIOL 1107 - Chapter 17

... -Prey vector: has transcription-activating ...
No Slide Title
No Slide Title

... Pease et al (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5022. ...
polymerase chain reaction
polymerase chain reaction

...  Your probe 1 will bind to a fragment of the DNA from library II.  Isolate this fragment  Make a probe, probe 2, for its 3’ end.  Expose probe 2 to the DNA from library 1 and this will bind further along the DNA, hence walking down the DNA fragment.  If you keep repeating this, you move all the ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... developing organism, but it is certain that in the formation of gametes for the next generation the normal pattern of imprinting is reimposed. Much information about DNA methylation and the epigenetic control of gene activity is now available in plants (Martienssen and Colot 2001). Also, in the last ...
Instructor`s Manual to accompany Principles of Life
Instructor`s Manual to accompany Principles of Life

... This common genetic code is a common language for evolution. The code is ancient and has remained intact throughout evolution. The common code also facilitates genetic engineering. Mutations can also be defined in terms of their effects on polypeptide sequences. Silent mutations have no effect on am ...
No Slide Title
No Slide Title

... can be changed in individuals through mutation and how DNA changes from generation to generation through recombination and independent assortment during meiosis and sexual reproduction. • For thousands of years humans have used selective breeding in agriculture, horticulture and what was once quaint ...
Organizing Protein Synthesis - Dallastown Area School District Moodle
Organizing Protein Synthesis - Dallastown Area School District Moodle

... cells would fall apart because the proteins were not being_______________________!! B. TRANSLATION (Protein Synthesis)-From RNA to Protein: 1. First codon of mRNA attaches to ________________. 2. ______ (transfer RNA)- each carries a specific amino acid; the tRNA anti-codon will pair up with its com ...
Key Area 4-6 HOMEWORK Marking Scheme
Key Area 4-6 HOMEWORK Marking Scheme

... 7 and so only slightly alter/alter few amino acids in the amino acid sequence of the protein 1 8 insertion/deletion affect many triplets/all codons after the mutation/are frame-shift mutations 1 9 and so affect many amino acids in a protein/all amino acids after the mutation 1 ...
Research Focused Undergraduate Education - GCG-42
Research Focused Undergraduate Education - GCG-42

Advances in the molecular ecology of foxes
Advances in the molecular ecology of foxes

... wheatbelt areas of Western Australia to use DNA analysis to estimate density and survival of foxes during a typical 1080 aerial baiting program. DNA was obtained from hair samples that were collected using hair snares. This analysis of hair samples provided significantly more individual ‘captures’ t ...
Genetic Engineering Aviation High School Living
Genetic Engineering Aviation High School Living

Gene 5102-96
Gene 5102-96

... examinase. There is a simple in vitro assay for the activity of examinase. The following list contains some of the items of evidence that led to confirmation that the segment indeed contains the gene for the examinase enzyme. However, each piece of evidence by itself was not sufficient evidence that ...
Lab5CysticFibroShort
Lab5CysticFibroShort

Cell cycle and Reproduction - River Dell Regional School District
Cell cycle and Reproduction - River Dell Regional School District

...  The relationship between a cell’s volume and its surface area is the key to understanding why cells must divide as ...
SAM Teachers Guide - RI
SAM Teachers Guide - RI

... The Proteins and Nucleic Acids activity focuses on the basic structure of protein, DNA  and RNA—the monomers, the distribution of charges and polarity, and how charged  surfaces contribute to their shape and function. Atomic Structure introduces students to  the positive and negative parts of atoms. ...
genetic code
genetic code

... Two '+' or two '-' mutants did not cancel Three '+' or three '-' mutants WERE able to cancel out each other, just like a '+' and a '-' this suggested a 'triplet' code-- 3 nucleic acids per amino acid '+' frameshift and '-' frameshift nearby gives mostly normal enzymes two '+' or two '-' enzymes coul ...
Unit Four: Genetics - Life Science Academy
Unit Four: Genetics - Life Science Academy

DNA cloning
DNA cloning

... • Removes the phosphate groups from the 5’ends of the vector DNA linearized by a single restriction enzyme to prevent the self-ligation of the vector DNA upon the followed ligation; • One phosphate is present to ligate one strand, the remaining nicks will be repaired by cellular mechanisms after tra ...
Tutorial: Protein Synthesis - Integrated DNA Technologies
Tutorial: Protein Synthesis - Integrated DNA Technologies

... The first feature of this generic gene that should be mentioned is that the part of the gene that codes for an amino acid sequence is not intact. The coding region is split into segments that code for protein, called exons, and segments that do not code for protein, called introns. The existence of ...
The Origin of Life on Earth by Leslie E. Orgel Growing evidence
The Origin of Life on Earth by Leslie E. Orgel Growing evidence

... of nucleic acids and proteins come into being? Anyone trying to solve this puzzle immediately encounters a paradox. Nowadays nucleic acids are synthesized only with the help of proteins, and proteins are synthesized only if their corresponding nucleotide sequence is present. It is extremely improbab ...
Biological Molecules
Biological Molecules

... Diversity results from the unique combination of these subunits How are macromolecules formed? Terms: Polymerization—chemical reactions that link two or more small molecules to form larger molecules with repeating structural units Condensation Reaction—polymerization reaction which form covalent lin ...
Horizontal Gene transfer
Horizontal Gene transfer

... genes encoding enzymes required for synthesis of AA, nucleotides, substances not added to minimal media ...
Protein Synthesis Card Sort
Protein Synthesis Card Sort

... aka “Transcription” of the DNA code to take out of the nucleus. This copy is called “mRNA” (messenger RNA). Thymine is replaced with Uracil. ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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