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DNA Replication
DNA Replication

... The DNA in chromosomes contains genetic instructions that regulate development, growth, and the metabolic activities of cells. The DNA instructions determine whether a cell will be that of a pea plant, a human, or some other organism, as well as establish specific characteristics of the cell in that ...
Editable PPT - Science Prof Online
Editable PPT - Science Prof Online

... Detail Diagram: Madprime; DNA & RNA Diagrams, BiologyCorner ...
Activity Title: Gummy Bear Population Genetics
Activity Title: Gummy Bear Population Genetics

... Point mutations occur when only one base is changed. This can cause problems (by changing the amino acid that a codon codes for) or not depending on where the change occurs and what the change is. Example: The cat ate the mat. Point Mutation: The cat tte the mat. So, to summarize, DNA mutations crea ...
DNA and Forensic Science
DNA and Forensic Science

... initially chosen for analysis. This cycle of heating and cooling is then repeated many times until an exponential rate of replication occurs and a very large amount of DNA containing the specific region is obtained. This enables the investigators to further manipulate or analyze this region of DNA w ...
Goal 3: The learner will develop an understanding of the continuity
Goal 3: The learner will develop an understanding of the continuity

... 3. a nitrogen base (adenine, cytosine, guanine, thymine) -Complementary base pairing: A-T, C-G - The two strands of the DNA molecule are held together by hydrogen bonds formed between complementary nitrogen bases. -DNA, like a blueprint, contains information for cells to function, and for the organi ...
PPT
PPT

...  Minute amounts of DNA may be used for amplification.  DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification.  Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions.  Commercia ...
Question 1
Question 1

... 6a. The water molecules are all located along the hydrophilic edges of the bases in the major and minor grooves or are interacting with the charged and polar sugar phosphate backbone. The waters are expelled from the hydrophobic interior of the DNA double helix which are occupied by the hydrophobic ...
What are transcription factors?
What are transcription factors?

...  Protein products made from genes will have specific functions in the cell. One type of protein product is called a transcription factor. Transcription factors are proteins with a specific job: they bind the regulatory/non-coding DNA of a gene which will then cause the gene (coding DNA) to be expre ...
National Exam
National Exam

... How  might  Phe1324  and  Ile1270  contribute  to  the  structure  and  function  of  the  Cas9  protein?  (2  pts)   These  sidechains  are  hydrophobic  in  nature  –  and  are  closely  packed  in  the  inside  of  a  sub-­‐domain ...
No Slide Title
No Slide Title

...  Minute amounts of DNA may be used for amplification.  DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification.  Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions.  Commercia ...
Plasmids - winterk
Plasmids - winterk

... They act as delivery vehicles, or vectors, to introduce foreign DNA into bacteria. Using plasmids for DNA delivery began in the 1970s when DNA from other organisms was first ‘cut and pasted’ into specific sites within the plasmid DNA. The modified plasmids were then reintroduced into bacteria. Decad ...
Basic Molecular Theory - American Society of Cytopathology
Basic Molecular Theory - American Society of Cytopathology

... • mRNA transcripts must be reduced to portions that  will be translated into proteins (exons) • Splicing:  a process by which the introns are removed  after transcription • Spliceosome: – RNA‐protein complex that can recognize short RNA  sequences (eg. GU and AG) that signal start and stop of an  in ...
ch11dna
ch11dna

...  Minute amounts of DNA may be used for amplification.  DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification.  Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions.  Commercia ...
A Picture`s Worth 1000 Words INTRODUCTION DNA fingerprinting
A Picture`s Worth 1000 Words INTRODUCTION DNA fingerprinting

... report. It seemed that doctors could perform a DNA test to determine if someone could inherit a disease called Huntington disease, which was very similar to JND. Alex was twenty-nine years old and recently married. He very much wanted to start a family but did not want to take a chance of passing on ...
Nutrigenomics and nutrigenetics – are they the keys for healthy
Nutrigenomics and nutrigenetics – are they the keys for healthy

... dietary signals that are detected by the cellular sensor systems (e.i. PPARγ and RXR receptors) that influence gene expression, protein synthesis and metabolite production. From this point of view genes are dietary targets. Patterns of gene expression, protein synthesis and metabolite production in ...
Biology GENETICS Practice Test with Answer Key
Biology GENETICS Practice Test with Answer Key

... protein. What is this process called? A. mutation B. synthesis C. translation D. transference 34. Which best shows the proper code-structure sequence in protein synthesis? A. DNA, mRNA, mRNA, polypeptide, enzyme B. DNA, mRNA, tRNA, polypeptide, enzyme C. enzyme, polypeptide, mRNA, mRNA, DNA D. mRNA, ...
Mutations Notes
Mutations Notes

... 1. To illustrate gene mutations, we’re going to use this sentence as an example: THEDOGBITTHECAT ___ ___ ___ ___ ___ ...
PowerPoint file
PowerPoint file

... processing algorithms and methods are used to study functional structures in the DNA. An appropriate mapping of the DNA sequence into one or more numerical sequences, enables the use of many digital signal processing tools. DNA Segment ...
Should I Use DNA Testing? - Beef Improvement Federation
Should I Use DNA Testing? - Beef Improvement Federation

pGLO2011 Wilkes
pGLO2011 Wilkes

... short segments of DNA containing a foreign or modified gene to living cells. Transformation- the uptake and expression of DNA by a living cell is the limiting factor in the genetic engineering of any species. Genes can be cut from human, animal, or plant DNA and placed inside bacteria. For example, ...
MITOSIS Introduction Objectives: The first objective is to appreciate
MITOSIS Introduction Objectives: The first objective is to appreciate

... the more the organism can grow and develop. Also, in certain parts of the body like inside the mouth or the skin, cells are constantly being destroyed. Because of mitosis, exact copies of those cells can quickly be created to maintain those parts of the body. Mitosis is very important to organisms t ...
Ribosomal Protein L11 HDR Plasmid (m): sc-426331
Ribosomal Protein L11 HDR Plasmid (m): sc-426331

PowerPoint PDF Printout
PowerPoint PDF Printout

... Process by which a DNA sequence is copied to produce a complementary mRNA strand. In other words, it is the transfer of genetic information from DNA into RNA. Like replication, but making RNA. Beginning of the process that ultimately leads to the translation of the genetic code (via mRNA) into a pro ...
Managing people in sport organisations: A strategic human
Managing people in sport organisations: A strategic human

... These are complementary and base pair by hydrogen bonding. The breaks in the DNA backbones are sealed by T4 DNA ligase, which hydrolyzes ATP to energize the reaction. Figure A RFLP Analysis DNA from related organisms shows small differences in sequence that cause changes in restriction sites. In the ...
Chapter 15 Instructor Manual
Chapter 15 Instructor Manual

... line machinery, and tRNA is the robot that delivers the amino acids from the supply room to the assembly line. Both transcription and translation are ultimately controlled by various assembly enzymes that recognize specific nucleotide sequences. The genetic code that translates base pair sequence in ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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