Issues in predicting protein function from sequence
... Although the same function or the same fold may have evolved more than once due to convergence, convergent evolution of sequences does not occur Domains possess single conformations ...
... Although the same function or the same fold may have evolved more than once due to convergence, convergent evolution of sequences does not occur Domains possess single conformations ...
The plastid division proteins, FtsZ1 and FtsZ2, differ in their
... division, we examined the effects of both FtsZ proteins by overproducing them in E. coli M15 by employing the expression vector PQE31. In this plasmid the protein is fused to a small histidine tag, and such small tags have been shown not to interfere with the biological activities of bacterial FtsZ ...
... division, we examined the effects of both FtsZ proteins by overproducing them in E. coli M15 by employing the expression vector PQE31. In this plasmid the protein is fused to a small histidine tag, and such small tags have been shown not to interfere with the biological activities of bacterial FtsZ ...
Nutrisi & Pertumbuhan Mikrobia
... Passive diffusion is the process in which molecules move from a region of higher concentration to one of lower concentration as a result of random thermal agitation. A few substances, such as glycerol, can cross the plasma membrane by passive diffusion. ...
... Passive diffusion is the process in which molecules move from a region of higher concentration to one of lower concentration as a result of random thermal agitation. A few substances, such as glycerol, can cross the plasma membrane by passive diffusion. ...
significance of the putative upstream polybasic nuclear localisation
... Interferon-gamma (IFNγ) accomplishes its multiple biological effects by activating the STAT transcription factors, which are translocated to the nucleus through a specific nuclear localization sequence(s) (NLS) located in the IFNγ molecule. Two putative NLS have been pointed out in the human interfe ...
... Interferon-gamma (IFNγ) accomplishes its multiple biological effects by activating the STAT transcription factors, which are translocated to the nucleus through a specific nuclear localization sequence(s) (NLS) located in the IFNγ molecule. Two putative NLS have been pointed out in the human interfe ...
L20_StvnWAT
... – Not all amino acids can be made into glucose • Glucogenic - can be made into glucose • Ketogenic - cannot be made into glucose – ~3g protein 1g glucose ...
... – Not all amino acids can be made into glucose • Glucogenic - can be made into glucose • Ketogenic - cannot be made into glucose – ~3g protein 1g glucose ...
Yeast SEC16 Gene Encodes a Multidomain Vesicle Coat Protein
... later in the secretory pathway (Kaiser and Schekman, 1990). These stage-specific genetic interactions show that at 25°C, sEcl6 alleles impair vesicle formation at the ER. Further, secl6 mutations are partially suppressed by overexpression of SAR1, a small GTP-binding protein that is required for ER ...
... later in the secretory pathway (Kaiser and Schekman, 1990). These stage-specific genetic interactions show that at 25°C, sEcl6 alleles impair vesicle formation at the ER. Further, secl6 mutations are partially suppressed by overexpression of SAR1, a small GTP-binding protein that is required for ER ...
emboj7601444-sup
... Wild-type bovine E2bCD (residues 162-421) fused at their amino-termini to maltosebinding protein (MBP), with the tobacco-etch virus (TEV) recognition sequence inserted in between the two moieties, was expressed in E. coli BL-21 (DE3) cells, similar to the method described previously (Wynn et al., 19 ...
... Wild-type bovine E2bCD (residues 162-421) fused at their amino-termini to maltosebinding protein (MBP), with the tobacco-etch virus (TEV) recognition sequence inserted in between the two moieties, was expressed in E. coli BL-21 (DE3) cells, similar to the method described previously (Wynn et al., 19 ...
integration from proteins to organs: the physiome project
... • Significant inflammatory response ...
... • Significant inflammatory response ...
PDF
... change in tissue-pH, a decrease during histolysis, and an increase during histogenesis and differentiation. Integral pH varies not less than one pH-unit during this time (Agrell, 1948). The proteolytic enzyme found is of the peptic type (Agrell, 19516), and thus it is activated with increasing histo ...
... change in tissue-pH, a decrease during histolysis, and an increase during histogenesis and differentiation. Integral pH varies not less than one pH-unit during this time (Agrell, 1948). The proteolytic enzyme found is of the peptic type (Agrell, 19516), and thus it is activated with increasing histo ...
McPherson, Selwyn-Lloyd: Investigations Into a Genetic Algorithm for Protein Sequences
... sequence problem (Figure 3). There is, however, a limit to pmut; values of 0.3 and 0.6 performed worse than pmut = 0.1, though pmut = 0.3 did better than smaller values. These findings are in contrast with the mutation probabilities commonly found in the literature. The argument that a mutation rate ...
... sequence problem (Figure 3). There is, however, a limit to pmut; values of 0.3 and 0.6 performed worse than pmut = 0.1, though pmut = 0.3 did better than smaller values. These findings are in contrast with the mutation probabilities commonly found in the literature. The argument that a mutation rate ...
Protein Metabolism
... Rate-limiting step: carbomyl-p-synthetase I, activated by N-acetyl-glu (synthesized greatly after ingestion of a protein-rich meal: AcetylCoA + Glu ...
... Rate-limiting step: carbomyl-p-synthetase I, activated by N-acetyl-glu (synthesized greatly after ingestion of a protein-rich meal: AcetylCoA + Glu ...
Biosynthesis of the dystonia-associated AAA ATPase torsinA at the
... Previous studies have found that multiple forms of torsinA are present in mammalian cells [4,5,10,11]. In order to characterize these, we first examined the biosynthesis of torsinA in a cell-free translation system. Four distinct membrane-associated products were generated when translation was carri ...
... Previous studies have found that multiple forms of torsinA are present in mammalian cells [4,5,10,11]. In order to characterize these, we first examined the biosynthesis of torsinA in a cell-free translation system. Four distinct membrane-associated products were generated when translation was carri ...
Nucleotide sequence of the genomic RNA of pepper mild mottle
... (Young et al., 1987; Goldbach & Wellink, 1988; Strauss & Strauss, 1988; Quadt & Jaspars, 1989). The alignment of the 126K/183K proteins of PMMV-S with those from the more closely related tobamoviruses (ToMV and TMV) shows that the sequence is well conserved along all the protein (Fig. 3), except for ...
... (Young et al., 1987; Goldbach & Wellink, 1988; Strauss & Strauss, 1988; Quadt & Jaspars, 1989). The alignment of the 126K/183K proteins of PMMV-S with those from the more closely related tobamoviruses (ToMV and TMV) shows that the sequence is well conserved along all the protein (Fig. 3), except for ...
CSCI 2951G: Guide to the proteins Code
... protein folding process is trying to minimize). Next is the current temperature. Next, “T adjust” displays by what fraction velocities have been rescaled to try to match the target temperature. (Initially, when friction is high, the temperature will be much lower than the target temperature, and “T ...
... protein folding process is trying to minimize). Next is the current temperature. Next, “T adjust” displays by what fraction velocities have been rescaled to try to match the target temperature. (Initially, when friction is high, the temperature will be much lower than the target temperature, and “T ...
molecular cell biology • molecular cell biology
... 20 amino acid (a.a.) Only glycine → symmetry ...
... 20 amino acid (a.a.) Only glycine → symmetry ...
4.Lect Carbon skeleton intro
... synthesize glucose and are termed glucogenic. while some are converted to acetylCoA (ketogenic amino acids) these CANNOT be used to synthesize glucose. Ketogenic amino acids can be converted to fatty acids for storage as triglyceride and later oxidation (fed state), or to ketone bodies (made in live ...
... synthesize glucose and are termed glucogenic. while some are converted to acetylCoA (ketogenic amino acids) these CANNOT be used to synthesize glucose. Ketogenic amino acids can be converted to fatty acids for storage as triglyceride and later oxidation (fed state), or to ketone bodies (made in live ...
Translation
... codon (mRNA) and anticodon of (tRNA). Sites include regions of large and small subunits of ribosome. ...
... codon (mRNA) and anticodon of (tRNA). Sites include regions of large and small subunits of ribosome. ...
Protein dynamics and proteolysis in plant vacuoles
... cleaved from the precursor and it enters the biosynthetic pathway (also called the secretion pathway). Large segments of the precursor correspond to the mature vacuolar protein. Precursors can contain more than one subunit of the mature protein (e.g. 2S and 11S storage proteins; Müntz, 1998) or eve ...
... cleaved from the precursor and it enters the biosynthetic pathway (also called the secretion pathway). Large segments of the precursor correspond to the mature vacuolar protein. Precursors can contain more than one subunit of the mature protein (e.g. 2S and 11S storage proteins; Müntz, 1998) or eve ...
Qualifying Liquid Co-Products
... Amino acid fermentation by-products result from processes designed to produce lysine and glutamic acid (or monosodium glutamate) for both animal and human food products. In these processes a high sugar substrate is inoculated with specialized microbial cultures which yield high concentrations of the ...
... Amino acid fermentation by-products result from processes designed to produce lysine and glutamic acid (or monosodium glutamate) for both animal and human food products. In these processes a high sugar substrate is inoculated with specialized microbial cultures which yield high concentrations of the ...
Tracing Protein Backbones in Electron Density Maps using a
... Unit cell may contain multiple copies of the protein ...
... Unit cell may contain multiple copies of the protein ...
Structure/activity studies of anti-inflammatory
... peptide was essential for these bioactivities but that the C-terminal K residue was unnecessary for inhibitory activity. We have now synthesized a new series of peptides which contain single residue substitutions at each position of the reference peptide, YYWIGIR-NH2, and have tested these peptides ...
... peptide was essential for these bioactivities but that the C-terminal K residue was unnecessary for inhibitory activity. We have now synthesized a new series of peptides which contain single residue substitutions at each position of the reference peptide, YYWIGIR-NH2, and have tested these peptides ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.