Isolation of Vibrio harveyi Acyl Carrier Protein and the fabG, acpP
... carrier essential for the synthesis of fatty acids, phospholipids, and other complex molecules in a variety of organisms. The prototypic ACP from Escherichia coli is a 9-kDa acidic protein (pI, 4.1) of 77 amino acids which carries fatty acids as thioester intermediates attached to a phosphopantethei ...
... carrier essential for the synthesis of fatty acids, phospholipids, and other complex molecules in a variety of organisms. The prototypic ACP from Escherichia coli is a 9-kDa acidic protein (pI, 4.1) of 77 amino acids which carries fatty acids as thioester intermediates attached to a phosphopantethei ...
Communication - Department of Molecular Physiology and Biophysics
... containing amino acids 1-246 shows no binding on syntrophin (Fig. 4a). This suggests that syntrophin may interact with utrophin at sites homologous to the corresponding sequence in dystrophin. Comparing the deduced protein sequences of dystrophin and utrophin, many amino acid residues are conserved ...
... containing amino acids 1-246 shows no binding on syntrophin (Fig. 4a). This suggests that syntrophin may interact with utrophin at sites homologous to the corresponding sequence in dystrophin. Comparing the deduced protein sequences of dystrophin and utrophin, many amino acid residues are conserved ...
Structure, Mechanism, and Disease Implications of Acetyl CoA
... Abstract: The ubiquitous acetyl-CoA carboxylase is a pivotal enzyme in the synthesis of fatty acids in both eukaryotes and prokaryotes. The importance of this enzyme is needed in the initiating reaction for synthesizing fatty acids, which are very important when used as fuel molecules and providing ...
... Abstract: The ubiquitous acetyl-CoA carboxylase is a pivotal enzyme in the synthesis of fatty acids in both eukaryotes and prokaryotes. The importance of this enzyme is needed in the initiating reaction for synthesizing fatty acids, which are very important when used as fuel molecules and providing ...
Slide 1
... • Carbohydrate attached to a lipid • Some are glycerol based, but most are sphingosine based • Glycosphingolipids • Cerebrosides • Gangliosides ...
... • Carbohydrate attached to a lipid • Some are glycerol based, but most are sphingosine based • Glycosphingolipids • Cerebrosides • Gangliosides ...
Association of CAD, a multifunctional protein involved in pyrimidine
... transfected with or without FLAG-mLST8 vector was subjected to immunoprecipitation with the anti-FLAG antibody, and the proteins eluted from resin with the FLAG peptide were visualized by silver staining after SDS-PAGE. FLAG-mLST8, mTOR, and the proteins recovered from the immunoprecipitate (No.1 to ...
... transfected with or without FLAG-mLST8 vector was subjected to immunoprecipitation with the anti-FLAG antibody, and the proteins eluted from resin with the FLAG peptide were visualized by silver staining after SDS-PAGE. FLAG-mLST8, mTOR, and the proteins recovered from the immunoprecipitate (No.1 to ...
Severe factor XI deficiency caused by a Gly555 to Glu mutation
... Gly193 in chymotrypsin, the prototype used to compare trypsin-like proteases [6], and is highly conserved among serine proteases [7–9]. Mutations at the corresponding residues in FIX [10] and FVII [11] cause CRM + hemophilia B and FVII deficiency, respectively. The amido nitrogen of Gly193 forms part ...
... Gly193 in chymotrypsin, the prototype used to compare trypsin-like proteases [6], and is highly conserved among serine proteases [7–9]. Mutations at the corresponding residues in FIX [10] and FVII [11] cause CRM + hemophilia B and FVII deficiency, respectively. The amido nitrogen of Gly193 forms part ...
Microsoft Word
... enzyme was associated with dual functions, i.e., proteolysis and chitosanolysis. Its Mr and N-terminal amino acid sequence, confirmed it to be pepsin. Use of active site specific inhibitors as well as covalent modification of the active site residues indicated the involvement of aspartic acid during ...
... enzyme was associated with dual functions, i.e., proteolysis and chitosanolysis. Its Mr and N-terminal amino acid sequence, confirmed it to be pepsin. Use of active site specific inhibitors as well as covalent modification of the active site residues indicated the involvement of aspartic acid during ...
PLP-dependent Enzymes: a Powerful Tool for - Beilstein
... that are not part of the organism’s physiology. This capability of an enzyme to catalyze alternative reactions (often different types of reactions with different substrates) is referred to as catalytic promiscuity [16, 17]. Catalytic promiscuity is believed to have played a fundamental role in diver ...
... that are not part of the organism’s physiology. This capability of an enzyme to catalyze alternative reactions (often different types of reactions with different substrates) is referred to as catalytic promiscuity [16, 17]. Catalytic promiscuity is believed to have played a fundamental role in diver ...
Functions of the liver Assessment and interpretation of liver function
... • Drugs– barbiturates, benzodiazepines, halogenated volatile anaesthetics, pethidine etc. ...
... • Drugs– barbiturates, benzodiazepines, halogenated volatile anaesthetics, pethidine etc. ...
ภาพนิ่ง 1
... • Experimentally, Km is a useful parameter for characterizing the number and/or types of substrates that a particular enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate- ...
... • Experimentally, Km is a useful parameter for characterizing the number and/or types of substrates that a particular enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate- ...
Perturbation of - Circulation Research
... achieved in a nonselective manner using pharmacological agents. An animal model of cardiomyocyte-restricted UPS functional impairment is currently lacking but would remarkably benefit the elucidation of pathophysiological significance of cardiac UPS dysfunction in cardiac pathogenesis. Ubiquitinatio ...
... achieved in a nonselective manner using pharmacological agents. An animal model of cardiomyocyte-restricted UPS functional impairment is currently lacking but would remarkably benefit the elucidation of pathophysiological significance of cardiac UPS dysfunction in cardiac pathogenesis. Ubiquitinatio ...
Characterization of Lipid Rafts from Medicago
... evaluate the contamination by other membranes. Measurement of the vanadate-sensitive ATPase activity showed a 7-fold enrichment in PM proteins after phase partition compared with the starting material consisting of a crude microsomal fraction; a classical ratio for this technique. The mitochondrial ...
... evaluate the contamination by other membranes. Measurement of the vanadate-sensitive ATPase activity showed a 7-fold enrichment in PM proteins after phase partition compared with the starting material consisting of a crude microsomal fraction; a classical ratio for this technique. The mitochondrial ...
discovery of new enzymes in extreme environments through
... Extremozymes expand the limits of biocatalysis – The information acquired from the study of extremozymes makes it possible to modify enzymes to improve their ranges of stability and activity (for industrial and medical applications) However, the organisms living in extreme environments are hard to b ...
... Extremozymes expand the limits of biocatalysis – The information acquired from the study of extremozymes makes it possible to modify enzymes to improve their ranges of stability and activity (for industrial and medical applications) However, the organisms living in extreme environments are hard to b ...
Amino acid Catabolism
... transferred to the -carbon atom of α-ketoglutarate, leaving behind the corresponding α-keto acid analog of the amino acid • The effect of transamination reactions is to collect the amino groups from many different amino acids in the form of L-glutamate. • The glutamate then functions as an amino gro ...
... transferred to the -carbon atom of α-ketoglutarate, leaving behind the corresponding α-keto acid analog of the amino acid • The effect of transamination reactions is to collect the amino groups from many different amino acids in the form of L-glutamate. • The glutamate then functions as an amino gro ...
Jacob Corn
... infection even further. First, while initially unfolded protein fragments similar in sequence to the beta-sheet rich portions of PrPSc exhibit spontaneous self-assembly into beta-sheet structures (Baskakov et al., 2000), full-length PrPSc produced in vitro is not sufficient to cause prion infection ...
... infection even further. First, while initially unfolded protein fragments similar in sequence to the beta-sheet rich portions of PrPSc exhibit spontaneous self-assembly into beta-sheet structures (Baskakov et al., 2000), full-length PrPSc produced in vitro is not sufficient to cause prion infection ...
Group Leader Training Materials
... Once in the small intestine, another enzyme known as Enterokinase activates Trypsinogen by cleaving it. The active enzyme formed by this reaction is known as Trypsin. Trypsin then activates other inactive forms of digestive enzymes (chymotrypsin, elastase, and carboxypeptidase) in the small intestin ...
... Once in the small intestine, another enzyme known as Enterokinase activates Trypsinogen by cleaving it. The active enzyme formed by this reaction is known as Trypsin. Trypsin then activates other inactive forms of digestive enzymes (chymotrypsin, elastase, and carboxypeptidase) in the small intestin ...
Part 8 - Southeast University
... • Function identification – about 70% of the genes of M. jannaschii were assigned a function using sequence similarity ...
... • Function identification – about 70% of the genes of M. jannaschii were assigned a function using sequence similarity ...
BMS 6204 MEDICAL BIOCHEMISTRY & GENETICS SPRING 2010
... 3.6 ATP: Do I really want that second serving of pasta? Let's do the math (anaerobic vs. aerobic) 3.7 Glucose Storage: glycogenesis/glycogenolysis Small groups (3.1) 3.8 Gluconeogenesis: Glucogenic and ketogenic amino acids Muscle Metabolism: Cori cycle 3.9 Urea Synthesis and Metabolism Q & A with L ...
... 3.6 ATP: Do I really want that second serving of pasta? Let's do the math (anaerobic vs. aerobic) 3.7 Glucose Storage: glycogenesis/glycogenolysis Small groups (3.1) 3.8 Gluconeogenesis: Glucogenic and ketogenic amino acids Muscle Metabolism: Cori cycle 3.9 Urea Synthesis and Metabolism Q & A with L ...
Development of Small Designer Aldolase Enzymes: Catalytic Activity
... immune diversity) and selection with designed small molecule compounds (5-7). For experimental purposes, small peptides are more useful and convenient than large proteins, as long as they provide the same function, because small peptides can be more easily prepared and their characterization is simp ...
... immune diversity) and selection with designed small molecule compounds (5-7). For experimental purposes, small peptides are more useful and convenient than large proteins, as long as they provide the same function, because small peptides can be more easily prepared and their characterization is simp ...
NO 2
... Asparagine and glutamine link carbon and nitrogen metabolism Asparagine serves not only as a protein precursor, but as a key compound for nitrogen transport and storage because of its stability and high nitrogen-tocarbon ratio (1:2). The major pathway for asparagine synthesis involves the trans ...
... Asparagine and glutamine link carbon and nitrogen metabolism Asparagine serves not only as a protein precursor, but as a key compound for nitrogen transport and storage because of its stability and high nitrogen-tocarbon ratio (1:2). The major pathway for asparagine synthesis involves the trans ...
of Pseudomonas aeruginosa - BMC Microbiology
... challenge. The resistance of this opportunist pathogen to β-lactam antibiotics is determined mainly by production of the inactivating enzyme AmpC, a class C cephalosporinase with a regulation system more complex than those found in members of the Enterobacteriaceae family. This regulatory system als ...
... challenge. The resistance of this opportunist pathogen to β-lactam antibiotics is determined mainly by production of the inactivating enzyme AmpC, a class C cephalosporinase with a regulation system more complex than those found in members of the Enterobacteriaceae family. This regulatory system als ...
Exam Name___________________________________
... 18) During the fed state, which of the following occur(s)? A) allosteric inhibition of carnitine acyltransferase I B) insulin stimulation of malonyl CoA formation C) insulin inhibition of the hydrolysis of stored triacylglycerols D) All of the above ...
... 18) During the fed state, which of the following occur(s)? A) allosteric inhibition of carnitine acyltransferase I B) insulin stimulation of malonyl CoA formation C) insulin inhibition of the hydrolysis of stored triacylglycerols D) All of the above ...
The investigation of enzymes structure, physical
... the rates at which all physiologic processes take place, enzymes occupy central roles in health and disease. While in health all physiologic processes occur in an ordered, regulated manner and homeostasis is maintained, homeostasis can be profoundly disturbed in pathologic states. For example, the s ...
... the rates at which all physiologic processes take place, enzymes occupy central roles in health and disease. While in health all physiologic processes occur in an ordered, regulated manner and homeostasis is maintained, homeostasis can be profoundly disturbed in pathologic states. For example, the s ...
V6-SecondaryStructur.. - Chair of Computational Biology
... helix residues that are correctly predicted, Q %prd2T percentage of all predicted TMH helix residues that are correctly predicted, Q%obs2N percentage of all observed non-TMH helix residues that are correctly predicted, Q%prd2N percentage of all predicted non-TMH helix residues that are correctly pre ...
... helix residues that are correctly predicted, Q %prd2T percentage of all predicted TMH helix residues that are correctly predicted, Q%obs2N percentage of all observed non-TMH helix residues that are correctly predicted, Q%prd2N percentage of all predicted non-TMH helix residues that are correctly pre ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.