Struct Bio 101
... Motif is a simple combination of a few secondary structures, that appear in several different proteins in nature. A collection of motifs forms a domain. Domain is a more complex combination of secondary structures. It has a very specific function (contains an active site). A protein may contain more ...
... Motif is a simple combination of a few secondary structures, that appear in several different proteins in nature. A collection of motifs forms a domain. Domain is a more complex combination of secondary structures. It has a very specific function (contains an active site). A protein may contain more ...
Protein sensing in living cells by molecular rotor
... Rapid live-cell imaging of MGMT activities with BG-CCVJ Since the uorescence of BG-CCVJ is activated only when it is covalently bound to MGMT, the low background signal of the probe should allow us to image the MGMT activity in cells without the washing operation so that high-throughput imaging of ...
... Rapid live-cell imaging of MGMT activities with BG-CCVJ Since the uorescence of BG-CCVJ is activated only when it is covalently bound to MGMT, the low background signal of the probe should allow us to image the MGMT activity in cells without the washing operation so that high-throughput imaging of ...
Full PDF
... (VL) combines with a variable domain of the heavy chain (V Hl in the antibody molecule to form the heterodimeric Fv fragment , the smallest immunoglobulin substructure that is fully competent to bind the antigen . A number of structures of immunoglobulin variable domains have been determined since t ...
... (VL) combines with a variable domain of the heavy chain (V Hl in the antibody molecule to form the heterodimeric Fv fragment , the smallest immunoglobulin substructure that is fully competent to bind the antigen . A number of structures of immunoglobulin variable domains have been determined since t ...
2nd CAT
... In an experiment carried out in your BCH 221 laboratory the following initial velocity was obtained when different amounts of substrate were added to a fixed amount of enzyme. The experiment was repeated again in presence of 0.00022M inhibitor. ...
... In an experiment carried out in your BCH 221 laboratory the following initial velocity was obtained when different amounts of substrate were added to a fixed amount of enzyme. The experiment was repeated again in presence of 0.00022M inhibitor. ...
Integrative Assignment - California State University
... It is a lot easier to work with peripheral membrane proteins than integral membrane proteins. Crystal structures have been solved for Cytochrome c from many organisms. ...
... It is a lot easier to work with peripheral membrane proteins than integral membrane proteins. Crystal structures have been solved for Cytochrome c from many organisms. ...
A Nascent Membrane Protein Is Located Adjacent to ER Membrane
... Lingappa, 1986). In contrast, the nonpolar transmembrane segments of membrane proteins (also termed "stop-transfer" sequences; Blobel, 1980) do not pass through the bilayer, but are integrated into the membrane. The stop-transfer sequence anchors the nascent polypeptide in the plane of the membrane ...
... Lingappa, 1986). In contrast, the nonpolar transmembrane segments of membrane proteins (also termed "stop-transfer" sequences; Blobel, 1980) do not pass through the bilayer, but are integrated into the membrane. The stop-transfer sequence anchors the nascent polypeptide in the plane of the membrane ...
Document
... but whose topologies, when deduced from their sequences, strongly suggest that they evolved after Archaea, Bacteria and Eukaryotes had already diversified. In other words, these universal genes appear to be younger than the taxonomic groups in which they are found today “ That is to say, there was a ...
... but whose topologies, when deduced from their sequences, strongly suggest that they evolved after Archaea, Bacteria and Eukaryotes had already diversified. In other words, these universal genes appear to be younger than the taxonomic groups in which they are found today “ That is to say, there was a ...
Enzymes Review Game with Answers 2014 2015
... B) Elevated body temperatures may denature enzymes. This would interfere with the cell's abilities to catalyze various reactions. C) Elevated body temperatures will increase the energy of activation needed to start various chemical reactions in the body. This will interfere with the ability of enzym ...
... B) Elevated body temperatures may denature enzymes. This would interfere with the cell's abilities to catalyze various reactions. C) Elevated body temperatures will increase the energy of activation needed to start various chemical reactions in the body. This will interfere with the ability of enzym ...
High carbohydrate diet : which reduces gluconeogenesis by
... It is a precursor of milk sugar (lactose) for lactating mammary gland. It serves as only fuel for skeletal muscles in anaerobic conditions. ...
... It is a precursor of milk sugar (lactose) for lactating mammary gland. It serves as only fuel for skeletal muscles in anaerobic conditions. ...
Production of Polyclonal Antibodies against Sucrose Transporter
... Sucrose transporter (SUT1) protein plays important roles in sucrose translocation from leaves to other organs in plants, therefore it is interested to study the existence of SUT1 protein in plants. Detection of SUT1 protein in plants can be done by using specific antibodies for that protein. This re ...
... Sucrose transporter (SUT1) protein plays important roles in sucrose translocation from leaves to other organs in plants, therefore it is interested to study the existence of SUT1 protein in plants. Detection of SUT1 protein in plants can be done by using specific antibodies for that protein. This re ...
Biocatalysis - School of Chemical Sciences
... importantly, uncontrolled metabolic processes may result in undesirable side reactions during cell growth. The accumulation of these undesirable products as well as desirable products may be toxic to the cell, and these products can be difficult to separate from the rest of the cell culture. Another ...
... importantly, uncontrolled metabolic processes may result in undesirable side reactions during cell growth. The accumulation of these undesirable products as well as desirable products may be toxic to the cell, and these products can be difficult to separate from the rest of the cell culture. Another ...
Metabolism of Glycerol
... • bile salts break fat globules into smaller particles called micelles in the small intestine. • pancreatic lipases hydrolyze ester bonds to form monoacylglycerols and fatty acids, which recombine in the intestinal lining. • phospholipids and proteins coat the fats, forming chylomicrons, which are t ...
... • bile salts break fat globules into smaller particles called micelles in the small intestine. • pancreatic lipases hydrolyze ester bonds to form monoacylglycerols and fatty acids, which recombine in the intestinal lining. • phospholipids and proteins coat the fats, forming chylomicrons, which are t ...
Overview of Metabolism - Chapter 4 - Formatted
... The network of metabolic pathways in a tiny cell is much more complicated and several times more efficient than any circuit you may have seen on a ‘chip’! The tremendous degree of integration and control required to keep these systems functioning normally and appropriately is one of the most fascina ...
... The network of metabolic pathways in a tiny cell is much more complicated and several times more efficient than any circuit you may have seen on a ‘chip’! The tremendous degree of integration and control required to keep these systems functioning normally and appropriately is one of the most fascina ...
How metabolites modulate metabolic flux
... interaction of metabolites with mRNA. Riboswitches are regulatory domains which can specifically bind certain metabolites. Binding of the effector induces a conformational change in the mRNA to activate or inactivate translation. This sensing is well known in bacteria, and recently a few examples ha ...
... interaction of metabolites with mRNA. Riboswitches are regulatory domains which can specifically bind certain metabolites. Binding of the effector induces a conformational change in the mRNA to activate or inactivate translation. This sensing is well known in bacteria, and recently a few examples ha ...
Characterization of Ubiquitin/Proteasome
... determine the activity of the complex. Hap4 is known to play a signaling role in response to environmental conditions; however, little is known about the regulation of Hap4 levels or how it responses to a cell’s functional state. The activity of the Hap2-5 complex is known to be reduced in respirato ...
... determine the activity of the complex. Hap4 is known to play a signaling role in response to environmental conditions; however, little is known about the regulation of Hap4 levels or how it responses to a cell’s functional state. The activity of the Hap2-5 complex is known to be reduced in respirato ...
chromatography - Bio-Rad
... protein, and immunoglobulins, predominantly IgG, contribute 10 – 25%. The high concentrations of both albumin and IgG obscure low-abundance serum proteins and limit the amount of total serum protein that can be resolved by two-dimensional (2-D) electrophoresis. The use of a chromatography system for ...
... protein, and immunoglobulins, predominantly IgG, contribute 10 – 25%. The high concentrations of both albumin and IgG obscure low-abundance serum proteins and limit the amount of total serum protein that can be resolved by two-dimensional (2-D) electrophoresis. The use of a chromatography system for ...
Bell Activity
... Acids taste sour, and most acids are poisonous. B. Acids Change Colors in Indicators A substance that changes color in the presence of an acid or base is an indicator. Bromthymol blue indicator ...
... Acids taste sour, and most acids are poisonous. B. Acids Change Colors in Indicators A substance that changes color in the presence of an acid or base is an indicator. Bromthymol blue indicator ...
Insulin action on skeletal muscle protein metabolism during
... Rapamycin had no effect on proteolysis. These observations provide evidence that PI 3’kinase and p70 sbx play a role in the regulation of muscle protein turnover by insulin in epitrochlearis muscle !gure 3) and that some mTOR-independent elements are also involved in this regulation. In contrast, th ...
... Rapamycin had no effect on proteolysis. These observations provide evidence that PI 3’kinase and p70 sbx play a role in the regulation of muscle protein turnover by insulin in epitrochlearis muscle !gure 3) and that some mTOR-independent elements are also involved in this regulation. In contrast, th ...
Biochemistry review
... eicosapentaenoic acid (EPA) and Omega 3s docosahexanoic acid (DHA) levels? a. glucosamine and chondroitin b. B complex with B6, B12 and folate c. cold water fish oil supplement d. ADEK, the fat soluble vitamins ...
... eicosapentaenoic acid (EPA) and Omega 3s docosahexanoic acid (DHA) levels? a. glucosamine and chondroitin b. B complex with B6, B12 and folate c. cold water fish oil supplement d. ADEK, the fat soluble vitamins ...
ARTÍCULOS
... of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin ␣-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence o ...
... of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin ␣-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence o ...
- ATS Journals
... is a hallmark of emphysema, we hypothesized that elastin gene mutations similar to those seen in ADCL may play a role in the development of COPD. Because our previous linkage studies did not demonstrate evidence for linkage near the ELN gene on chromosome 7, we also hypothesized that any functional ...
... is a hallmark of emphysema, we hypothesized that elastin gene mutations similar to those seen in ADCL may play a role in the development of COPD. Because our previous linkage studies did not demonstrate evidence for linkage near the ELN gene on chromosome 7, we also hypothesized that any functional ...
Biochemical studies on a versatile esterase that is most catalytically
... lipase-like proteins from the α/β hydrolase family that can hydrolyse both C-C and C-O bonds may exist in nature at much higher levels than previously thought (Alcaide et al., 2013). From an ecological perspective, such proteins may contribute to global carbon cycling processes for complex substrate ...
... lipase-like proteins from the α/β hydrolase family that can hydrolyse both C-C and C-O bonds may exist in nature at much higher levels than previously thought (Alcaide et al., 2013). From an ecological perspective, such proteins may contribute to global carbon cycling processes for complex substrate ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.