The X-ray Crystal Structures of Human
... the pET-28b(⫹)-pmm1 plasmid. Transformed cells (4L) were grown at 37 °C in Luria broth (or minimal medium containing selenomethionine) containing 50 g/ml ampicillin and induced for 4 h with 0.4 mM isopropyl -D-thiogalactopyranoside. Cells were harvested by centrifugation and the pellet (11 g) was ...
... the pET-28b(⫹)-pmm1 plasmid. Transformed cells (4L) were grown at 37 °C in Luria broth (or minimal medium containing selenomethionine) containing 50 g/ml ampicillin and induced for 4 h with 0.4 mM isopropyl -D-thiogalactopyranoside. Cells were harvested by centrifugation and the pellet (11 g) was ...
video slide - Point Pleasant Beach School District
... Steroids • Lanolin- Commercially refined from sheep's wool. Humans have a small amount of lanolin in the hair and skin; lanolin helps give these structures flexibility. ...
... Steroids • Lanolin- Commercially refined from sheep's wool. Humans have a small amount of lanolin in the hair and skin; lanolin helps give these structures flexibility. ...
PCNA Protein Expression during Spermatogenesis of the
... mRNA was responsible for the production of the two PCNA proteins. To further characterize these two PCNA proteins, we used antibodies that recognized different epitopes of eel PCNA (amino acids 100-120, 180-197, and 242-260) and rat recombinant PCNA (amino acids 112-121 and 181-195). All of the anti ...
... mRNA was responsible for the production of the two PCNA proteins. To further characterize these two PCNA proteins, we used antibodies that recognized different epitopes of eel PCNA (amino acids 100-120, 180-197, and 242-260) and rat recombinant PCNA (amino acids 112-121 and 181-195). All of the anti ...
Vesicle-mediated Protein Transport: Regulatory
... transformants were screened for mutants that exhibited wildtype vacuolar protein sorting at 26°C but missorted vacuolar proteins at 37°C. Analysis of 50,000 yeast transformants yielded 15 candidate vps34~:(tsf = temperature for function; for an example, see Herman et al., 1991b) alleles. The plasmid ...
... transformants were screened for mutants that exhibited wildtype vacuolar protein sorting at 26°C but missorted vacuolar proteins at 37°C. Analysis of 50,000 yeast transformants yielded 15 candidate vps34~:(tsf = temperature for function; for an example, see Herman et al., 1991b) alleles. The plasmid ...
Project One: Identification of unknown mutants in the... Overview Neurospora crassa
... In this lab, unknowns will be provided that are either have a complete set of functional arginine biosynthetic enzymes (wild type) or are defective in one of these enzymes. The goal in the project is to identify these unknowns as being either wild type of one of the arg mutants which include arg1, a ...
... In this lab, unknowns will be provided that are either have a complete set of functional arginine biosynthetic enzymes (wild type) or are defective in one of these enzymes. The goal in the project is to identify these unknowns as being either wild type of one of the arg mutants which include arg1, a ...
NME2.28: fat and carbohydrate metabolism in the
... Pyruvate from glycolysis enters the hepatocyte/adipocyte mitochondria o Pyruvate is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) o Acetyl-CoA enters the first part of the TCA cycle (see NME 2.31) o Acetyl-CoA with oxaloacetate is converted to citrate o Citrate is exported from the mitocho ...
... Pyruvate from glycolysis enters the hepatocyte/adipocyte mitochondria o Pyruvate is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) o Acetyl-CoA enters the first part of the TCA cycle (see NME 2.31) o Acetyl-CoA with oxaloacetate is converted to citrate o Citrate is exported from the mitocho ...
Functional dissection of the baculovirus late expression factor
... The AcMNPV lef-8 gene was first discovered as one of 19 genes required for the expression of late and very late genes in SF-21 insect cells (Passarelli et al., 1994). It encodes a protein, LEF-8, that predicts a 102 kDa product with a conserved C-terminally positioned sequence motif of 13 amino acid ...
... The AcMNPV lef-8 gene was first discovered as one of 19 genes required for the expression of late and very late genes in SF-21 insect cells (Passarelli et al., 1994). It encodes a protein, LEF-8, that predicts a 102 kDa product with a conserved C-terminally positioned sequence motif of 13 amino acid ...
Performance - Fuller Laboratories
... assay (IFA) for Ehrlichia canis was described in the literature in 19721 and has served thereafter as a gold standard for serodiagnosis. The Fuller Laboratories test uses the Oklahoma/LSU isolate, grown in the DH82 canine macrophage cell line, as substrate. Antibody detection becomes detectable at a ...
... assay (IFA) for Ehrlichia canis was described in the literature in 19721 and has served thereafter as a gold standard for serodiagnosis. The Fuller Laboratories test uses the Oklahoma/LSU isolate, grown in the DH82 canine macrophage cell line, as substrate. Antibody detection becomes detectable at a ...
c Syun-Ru Yeh‡ and Denis L. Rousseau
... FIG. 3. Time course for the populations of the various hemeligand coordinated states during the unfolding of cyt c determined from tryptophan fluorescence and resonance Raman scattering measurements. The folded protein at pH 5.9 was unfolded at pH 3.8 (A) and at pH 5.8 (B) in 4.0 M GdnHCl. The magen ...
... FIG. 3. Time course for the populations of the various hemeligand coordinated states during the unfolding of cyt c determined from tryptophan fluorescence and resonance Raman scattering measurements. The folded protein at pH 5.9 was unfolded at pH 3.8 (A) and at pH 5.8 (B) in 4.0 M GdnHCl. The magen ...
Amino acid composition of pig meat in relation to live weight and sex
... From the nutritional aspect they are rightly regarded as the most valuable components of meat. They are “fully-valuable” – containing all essential amino acids. According to Šubrt et al. (2002) also the fat has to be considered as an indispensable part of nutrition that cannot be replaced by any oth ...
... From the nutritional aspect they are rightly regarded as the most valuable components of meat. They are “fully-valuable” – containing all essential amino acids. According to Šubrt et al. (2002) also the fat has to be considered as an indispensable part of nutrition that cannot be replaced by any oth ...
Isolation and Characterization of Protease Inhibitors from Animal
... Protease inhibitors (PIs) are ubiquitous in nature and are produced by diverse animals as well as plants. There are different types of proteases such as cysteine proteases (such as papain from papayas), metalloproteases (such as thermolysin from bacteria), serine proteases (such as trypsin, chymotry ...
... Protease inhibitors (PIs) are ubiquitous in nature and are produced by diverse animals as well as plants. There are different types of proteases such as cysteine proteases (such as papain from papayas), metalloproteases (such as thermolysin from bacteria), serine proteases (such as trypsin, chymotry ...
(Enzymes Lecture Notes).
... o view the lysozyme-substrate complex. Explore various settings, and observe the fit between enzyme and substrate. ...
... o view the lysozyme-substrate complex. Explore various settings, and observe the fit between enzyme and substrate. ...
Table 3S
... positive activator of the divergent ytmI operon (although this operon was not induced under the tested conditions) which is apparently involved in some aspect of sulfur assimilation (Coppee et al., 2001). The hisZ operon encodes the major enzymes for His biosynthesis and YuiF has recently been propo ...
... positive activator of the divergent ytmI operon (although this operon was not induced under the tested conditions) which is apparently involved in some aspect of sulfur assimilation (Coppee et al., 2001). The hisZ operon encodes the major enzymes for His biosynthesis and YuiF has recently been propo ...
paper describing HSSP
... Link between structure and sequence databases. Fortunately, many proteins in the database of known sequences are similar in sequence to a protein of known structure and this fact can be exploited to close the gap in the size of the two databases. For example, there are currently more than 100 known ...
... Link between structure and sequence databases. Fortunately, many proteins in the database of known sequences are similar in sequence to a protein of known structure and this fact can be exploited to close the gap in the size of the two databases. For example, there are currently more than 100 known ...
Secondary structure prediction method (SOPMA)
... a normal house cleaning process in healthy cells. In cancer cells the process is hijacked. The fact that Bromelain can turn the tables on cancer cells is quite interesting. ...
... a normal house cleaning process in healthy cells. In cancer cells the process is hijacked. The fact that Bromelain can turn the tables on cancer cells is quite interesting. ...
Chapter 23
... Coupled Reactions Coupled reactions are pairs of reactions that occur together. The energy released by one reaction is absorbed by the other reaction. ...
... Coupled Reactions Coupled reactions are pairs of reactions that occur together. The energy released by one reaction is absorbed by the other reaction. ...
macromolecules test 1
... Application of biological concepts and evidence from investigations to solve problems in new and familiar contexts. A2 Use of appropriate biological terms, conventions, formulae, and equations. A3 Demonstration of skills in individual and collaborative work. Knowledge and Understanding The specific ...
... Application of biological concepts and evidence from investigations to solve problems in new and familiar contexts. A2 Use of appropriate biological terms, conventions, formulae, and equations. A3 Demonstration of skills in individual and collaborative work. Knowledge and Understanding The specific ...
GABA(A) Receptor Family
... neurons by interacting with the GABA(A) receptor on these cells1 There are two major types of GABA receptors: the ionotropic GABA(A) and the metabotropic GABA(B) receptors. GABA(A)receptor belongs to the ligand gated ion channel superfamily.1,2 It is a heteropentamer, with all of its five subunits c ...
... neurons by interacting with the GABA(A) receptor on these cells1 There are two major types of GABA receptors: the ionotropic GABA(A) and the metabotropic GABA(B) receptors. GABA(A)receptor belongs to the ligand gated ion channel superfamily.1,2 It is a heteropentamer, with all of its five subunits c ...
SUPPLEMENTARY MATERIAL Title: Luteolin attenuate the D
... Japan). All other reagents used in the experiments were of analytical grade and purity. 1.2 Animal and tissue preparation Adult male Kunming mice (6 weeks old, weighing 18-23g) were randomized into 4 groups (n = 12 each): Group 1 (control group): mice received daily intraperitoneally injected of sal ...
... Japan). All other reagents used in the experiments were of analytical grade and purity. 1.2 Animal and tissue preparation Adult male Kunming mice (6 weeks old, weighing 18-23g) were randomized into 4 groups (n = 12 each): Group 1 (control group): mice received daily intraperitoneally injected of sal ...
Chapter 6. Metabolism & Enzymes
... due to fit between active site & substrate substrates held in active site by weak interactions ...
... due to fit between active site & substrate substrates held in active site by weak interactions ...
Figure 10.10 Deciphering the Genetic Code In 1961, Nirenberg and
... all of the bases were uracil (poly U). In this experiment, the scientists prepared a bacterial extract that contained all of the components needed to translate proteins and then added the mRNA homopolymer. Results showed that the cell-free extract produced a polypeptide composed entirely of the amin ...
... all of the bases were uracil (poly U). In this experiment, the scientists prepared a bacterial extract that contained all of the components needed to translate proteins and then added the mRNA homopolymer. Results showed that the cell-free extract produced a polypeptide composed entirely of the amin ...
SQA Higher Biology Unit 1 Cell Biology
... ¯ Some organisms are unicellular. ¯ Unicellular organisms can be either plant or animal cells. ¯ Some organisms are multicellular. ¯ Multicellular organisms can be either plants or animals. ¯ Multicellular organisms are organised into tissues, organs and systems, to function more effectively. ...
... ¯ Some organisms are unicellular. ¯ Unicellular organisms can be either plant or animal cells. ¯ Some organisms are multicellular. ¯ Multicellular organisms can be either plants or animals. ¯ Multicellular organisms are organised into tissues, organs and systems, to function more effectively. ...
Chapter 6. Metabolism & Enzymes
... active site binds substrate & puts stress on bonds that must be broken, making it easier to separate molecules ...
... active site binds substrate & puts stress on bonds that must be broken, making it easier to separate molecules ...
Gene Section CDK4 (cyclin-dependent kinase 4) Atlas of Genetics and Cytogenetics
... Local order: Telomeric to the OS9 (amplified in osteosarcoma 9), CENTG1 (centaurin, gamma 1) and TSPAN31 (tetraspanin 31, SAS) genes. Centromeric to the MARCH9 (membrane-associated ring finger (C3HC4) 9), CYP27B1 (cytochrome P450, family 27, subfamily B, polypeptide 1) and METTL1 (methyltransferase ...
... Local order: Telomeric to the OS9 (amplified in osteosarcoma 9), CENTG1 (centaurin, gamma 1) and TSPAN31 (tetraspanin 31, SAS) genes. Centromeric to the MARCH9 (membrane-associated ring finger (C3HC4) 9), CYP27B1 (cytochrome P450, family 27, subfamily B, polypeptide 1) and METTL1 (methyltransferase ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.