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... media; 25 μg/ml barely retards growth of cells without plasmid in rich media) Chloramphenicol is selective for the RIL plasmid (Stratagene Codon Plus) The expression strains we have tried can be grown to saturation in P-0.5G or PA0.5G with little if any loss of inducible plasmid. Cultures grown at 3 ...
... media; 25 μg/ml barely retards growth of cells without plasmid in rich media) Chloramphenicol is selective for the RIL plasmid (Stratagene Codon Plus) The expression strains we have tried can be grown to saturation in P-0.5G or PA0.5G with little if any loss of inducible plasmid. Cultures grown at 3 ...
Leukaemia Section t(4;17)(q12;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... amino-terminal amino acids of FIP1L, including the FIP homology domain and 403 carboxyl-terminal amino acids of RARA, including the DNA and ligand binding domains, with replacement of FIP1L1 amino acid 429 (Valine) and RARA amino acid 60 (Threonine) into an Alanine. Oncogenesis All known chimeric RA ...
... amino-terminal amino acids of FIP1L, including the FIP homology domain and 403 carboxyl-terminal amino acids of RARA, including the DNA and ligand binding domains, with replacement of FIP1L1 amino acid 429 (Valine) and RARA amino acid 60 (Threonine) into an Alanine. Oncogenesis All known chimeric RA ...
Cloning and sequence analysis of cnaA gene encoding the catalytic
... to study its function in detail. While earlier reports of calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction ...
... to study its function in detail. While earlier reports of calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction ...
A new type of plant chitinase containing LysM domains from a fern
... Fig. 4. Alignment of amino acid sequences from domains of PrChi-A with those from corresponding regions of several proteins. (A) Alignment of sequences from LysM domains of PrChi-A with those of LysM-containing proteins. (B) Alignment of the sequence from a catalytic domain of PrChi-A with that from ...
... Fig. 4. Alignment of amino acid sequences from domains of PrChi-A with those from corresponding regions of several proteins. (A) Alignment of sequences from LysM domains of PrChi-A with those of LysM-containing proteins. (B) Alignment of the sequence from a catalytic domain of PrChi-A with that from ...
Investigation of asparagine deamidation in a SOD1
... Asp/isoAsp isomers and therefore may be useful in identification of the deamidation site. There are also two indirect proofs for the presence of isoAsp residue employed in this study: termination of the Edman degradation reaction at isoAsp and resistance to cleavage by endoproteinase Asp-N (Kameoka ...
... Asp/isoAsp isomers and therefore may be useful in identification of the deamidation site. There are also two indirect proofs for the presence of isoAsp residue employed in this study: termination of the Edman degradation reaction at isoAsp and resistance to cleavage by endoproteinase Asp-N (Kameoka ...
Development of antifertility vaccine using sperm specific proteins
... cDNA for human sperm membrane protein (hSMP-1) of 55kDa. It did not show sequence homology with ...
... cDNA for human sperm membrane protein (hSMP-1) of 55kDa. It did not show sequence homology with ...
the structure and function of macromolecules
... the 40-50 common monomers and other rarer ones. • These monomers can be connected in various combinations like the 26 letters in the alphabet can be used to create a great diversity of words. • Biological molecules are even more diverse. Copyright © 2002 Pearson Education, Inc., publishing as Benjam ...
... the 40-50 common monomers and other rarer ones. • These monomers can be connected in various combinations like the 26 letters in the alphabet can be used to create a great diversity of words. • Biological molecules are even more diverse. Copyright © 2002 Pearson Education, Inc., publishing as Benjam ...
Proteomic Approach to Identify Novel
... of Arabidopsis mitochondria from green tissue, several available protocols were tested but turned out to be of limited suitability. Arabidopsis mitochondria and chloroplasts are not well resolved on the threestep Percoll (Amersham Pharmacia Biotech, Piscataway, NJ) gradients often used for plant mit ...
... of Arabidopsis mitochondria from green tissue, several available protocols were tested but turned out to be of limited suitability. Arabidopsis mitochondria and chloroplasts are not well resolved on the threestep Percoll (Amersham Pharmacia Biotech, Piscataway, NJ) gradients often used for plant mit ...
Dot Plot - APBioNET
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
Defective intestinal amino acid absorption in Ace2 null mice
... model has been described recently, showing that this transporter is essential for normal body weight gain and optimal growth control (7). The kidney-specific lack of B0AT1 was reported in mice lacking partner protein Tmem27 (15, 22). These mice were shown to display a massive aminoaciduria caused by ...
... model has been described recently, showing that this transporter is essential for normal body weight gain and optimal growth control (7). The kidney-specific lack of B0AT1 was reported in mice lacking partner protein Tmem27 (15, 22). These mice were shown to display a massive aminoaciduria caused by ...
Engineering of cyclodextrin glycosyltransferase reaction and
... K(1^4) bonds. Cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) is a unique member of the K-amylase family of glycosyl hydrolases with a low hydrolytic activity. CGTase mainly catalyses transglycosylation reactions and is usually considered to be an exo-acting enzyme, unable to bypass branchin ...
... K(1^4) bonds. Cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) is a unique member of the K-amylase family of glycosyl hydrolases with a low hydrolytic activity. CGTase mainly catalyses transglycosylation reactions and is usually considered to be an exo-acting enzyme, unable to bypass branchin ...
The Alzheimer Aβ Peptide: Identification of Properties Distinctive for Toxic Prefibrillar Species
... β-sheets, the β-strands are held together with hydrogen bonds, resulting in a very rigid structure. The β-strands in a β-sheet are not necessarily close to each other in the amino acid chain. The final three-dimensional structure of a protein when it is properly folded is referred to as the tertiar ...
... β-sheets, the β-strands are held together with hydrogen bonds, resulting in a very rigid structure. The β-strands in a β-sheet are not necessarily close to each other in the amino acid chain. The final three-dimensional structure of a protein when it is properly folded is referred to as the tertiar ...
Influence of bovine serum albumin (BSA)
... Protein surfactant interactions have been studied from last century. Anson [1] recognized that proteins are denatured by synthetic surfactants. A renewed interest in this field during the past decade can be attributed to the availability of new experimental techniques to study these mixtures and the ...
... Protein surfactant interactions have been studied from last century. Anson [1] recognized that proteins are denatured by synthetic surfactants. A renewed interest in this field during the past decade can be attributed to the availability of new experimental techniques to study these mixtures and the ...
introduction figures
... Figure 4: Ribbon diagram of the catalytic fragment of PARP-1 in complex with NAD+. The core of the C-terminal domain is formed by a five-stranded antiparallel bsheet and a four-stranded mixed b-sheet. The NAD+ binds in the cleft at the junction of the two central b-sheets shown in red where it is li ...
... Figure 4: Ribbon diagram of the catalytic fragment of PARP-1 in complex with NAD+. The core of the C-terminal domain is formed by a five-stranded antiparallel bsheet and a four-stranded mixed b-sheet. The NAD+ binds in the cleft at the junction of the two central b-sheets shown in red where it is li ...
Key Residues Controlling Binding of Diverse Ligands to Human
... and the nicotine-derived compounds cotinine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). CYP2A13 metabolizes nicotine and cotinine with 23- and 15-fold higher catalytic efficiency (kcat/Km) than CYP2A6, respectively (Bao et al., 2005). In addition, the metabolism of NNK by CYP2A13 occur ...
... and the nicotine-derived compounds cotinine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). CYP2A13 metabolizes nicotine and cotinine with 23- and 15-fold higher catalytic efficiency (kcat/Km) than CYP2A6, respectively (Bao et al., 2005). In addition, the metabolism of NNK by CYP2A13 occur ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.