Aalborg Universitet Christiansen, Gunna; Sennels, Lau; Stensballe, Allan; Birkelund, Svend
... polyacrylamide gel electrophoresis (2D-PAGE) of in vivo S35 labelled proteins. Hereby we showed that among the first proteins to be synthesized were the S1 ribosomal protein, the GroEl-like (HSP60) protein and the DnaK-like protein identified by immunoblotting (Lundemose et sl. 1990). Later, when th ...
... polyacrylamide gel electrophoresis (2D-PAGE) of in vivo S35 labelled proteins. Hereby we showed that among the first proteins to be synthesized were the S1 ribosomal protein, the GroEl-like (HSP60) protein and the DnaK-like protein identified by immunoblotting (Lundemose et sl. 1990). Later, when th ...
Chapter summaries
... 10. The helix is characterized by dimensions such as pitch (5.4Å), the translation distance (1.5Å), and the number of residues per turn (3.6). It is stabilized by hydrogen bonds orientated parallel to the helix axis and formed between the CO and NH groups of residues separated by four intervening ...
... 10. The helix is characterized by dimensions such as pitch (5.4Å), the translation distance (1.5Å), and the number of residues per turn (3.6). It is stabilized by hydrogen bonds orientated parallel to the helix axis and formed between the CO and NH groups of residues separated by four intervening ...
(1) Kinetic Studies -the type of information available from kinetic
... -plots of rxn rate versus pH makes it possible to deduce the pKa values of the side chains involved in catalysis -caveat: microenvironment of a side chain can shift the pKa of the side chain by up to four units from the value for the free amino acid -approach used to implicate two His side chains a ...
... -plots of rxn rate versus pH makes it possible to deduce the pKa values of the side chains involved in catalysis -caveat: microenvironment of a side chain can shift the pKa of the side chain by up to four units from the value for the free amino acid -approach used to implicate two His side chains a ...
The Pentatricopeptide Repeat Protein OTP87 Is Essential for RNA
... Is the paper clear and well written? ...
... Is the paper clear and well written? ...
translation
... A. Amino acids do not have direct affinity for nucleic acids 1. Therefore, mRNA can not directly serve as a template for protein synthesis 2. There must exist “adapter molecules” which can read the RNA sequence (codons) and bring with it the correct amino acids a) This is the function of tRNA molecu ...
... A. Amino acids do not have direct affinity for nucleic acids 1. Therefore, mRNA can not directly serve as a template for protein synthesis 2. There must exist “adapter molecules” which can read the RNA sequence (codons) and bring with it the correct amino acids a) This is the function of tRNA molecu ...
glyco revision 2004
... • Terminal carbohydrate sequence found on protein and lipid • Weak monomeric affinity but fast Kon- and Koff-rates • Multimeric interactions increase affinity • Bonds have a low fractional spring slippage, which means that as the bond is subjected to strain it has a low tendency to break. ...
... • Terminal carbohydrate sequence found on protein and lipid • Weak monomeric affinity but fast Kon- and Koff-rates • Multimeric interactions increase affinity • Bonds have a low fractional spring slippage, which means that as the bond is subjected to strain it has a low tendency to break. ...
Viral Structure Lec. 2
... – Capsid is the storage site for genome – Many capsids have a ‘shell’ structure – Genome + Capsid = Nucleocapsid – Capsid is made up of polymeric proteins to conserve genome • Ex. 5 Kb genome requires 30,000 a/a capsid, which means 90 Kb genome just for capsid!! • Solution: use multiple copies of sa ...
... – Capsid is the storage site for genome – Many capsids have a ‘shell’ structure – Genome + Capsid = Nucleocapsid – Capsid is made up of polymeric proteins to conserve genome • Ex. 5 Kb genome requires 30,000 a/a capsid, which means 90 Kb genome just for capsid!! • Solution: use multiple copies of sa ...
In experiments with a 3 base codon system it was shown that the
... form of the gene to a mutant form. A reversion mutation restores either the original DNA sequence of a gene or restores the function of the gene. Example of this is if the forward mutation was a base deletion (frameshift) a base insertion would be a ...
... form of the gene to a mutant form. A reversion mutation restores either the original DNA sequence of a gene or restores the function of the gene. Example of this is if the forward mutation was a base deletion (frameshift) a base insertion would be a ...
DNA and Translation Gene
... • Every DNA gene codes for a specific protein • Codon/anticodon match guarantees proper amino acid • Many amino acids link to make one protein ...
... • Every DNA gene codes for a specific protein • Codon/anticodon match guarantees proper amino acid • Many amino acids link to make one protein ...
translation
... DNA -> RNA -> Protein -> Trait TRANSCRIPTION: mRNA for a gene is made from one strand of DNA. The mRNA leaves the nucleus and goes to the ribosome. TRANSLATION: In the ribosome, tRNAs match up with their codons in the mRNA. The backsides of the tRNAs have specific amino acids attached to t ...
... DNA -> RNA -> Protein -> Trait TRANSCRIPTION: mRNA for a gene is made from one strand of DNA. The mRNA leaves the nucleus and goes to the ribosome. TRANSLATION: In the ribosome, tRNAs match up with their codons in the mRNA. The backsides of the tRNAs have specific amino acids attached to t ...
Identification of soil bacteria belonging to the phylum Acidobacteria
... Universidade Católica de Brasília, DF, Brasil. Recently, it has been shown that bacterial species of the phylum Acidobacteria are abundant in Cerrado soil, representing more than 50% of total 16S rRNA sequences obtained. These bacteria are expected to play an important role in nutrient cycling in th ...
... Universidade Católica de Brasília, DF, Brasil. Recently, it has been shown that bacterial species of the phylum Acidobacteria are abundant in Cerrado soil, representing more than 50% of total 16S rRNA sequences obtained. These bacteria are expected to play an important role in nutrient cycling in th ...
Document
... Rapid and efficient communication between neurons of the brain occurs at specialized cellular structures called synapses. An important molecular component of synapses is the protein piccolo. Piccolo is a large (>550 kDa) multi-domain protein and a member of the family of proteins involved in the org ...
... Rapid and efficient communication between neurons of the brain occurs at specialized cellular structures called synapses. An important molecular component of synapses is the protein piccolo. Piccolo is a large (>550 kDa) multi-domain protein and a member of the family of proteins involved in the org ...
Supplementary Tables and Figures Legends (doc 39K)
... described (Rix, 2007). The resulting matrices were exposed either to K562 (Table S3a) or KU812 (Table S3b) total cell lysates. All proteins identified in each experiment are shown in the list. Proteins are sorted by the number of times identified across the five drugs. The higher the incidence among ...
... described (Rix, 2007). The resulting matrices were exposed either to K562 (Table S3a) or KU812 (Table S3b) total cell lysates. All proteins identified in each experiment are shown in the list. Proteins are sorted by the number of times identified across the five drugs. The higher the incidence among ...
MATERIALS AND METHODS Materials All chemicals used in the
... CGH buffer at 25 ºC at a flow rate of 0.3 mL/min, with detection at 280 nm. The column was calibrated with standard molecular weight markers. Circular Dichroism (CD) measurements- CD measurements were made on JASCO J810 spectropolarimeter calibrated with ammonium (+)-10-camphorsulfonate with 6 µM p ...
... CGH buffer at 25 ºC at a flow rate of 0.3 mL/min, with detection at 280 nm. The column was calibrated with standard molecular weight markers. Circular Dichroism (CD) measurements- CD measurements were made on JASCO J810 spectropolarimeter calibrated with ammonium (+)-10-camphorsulfonate with 6 µM p ...
Gene expression (Protein synthesis )
... The enzyme peptidyltransferase catalyzes the formation of the peptide bond. ...
... The enzyme peptidyltransferase catalyzes the formation of the peptide bond. ...
A1982NK48200001
... the uptake of 02 do not work because 2the changes are so slow that days or weeksof incubation are needed. If the microbes are concentrated by filtration or even placed in a bottle for more than a few hours then communities and rates change. “Richard Wright and I, young postdoctoral fellows at Uppsal ...
... the uptake of 02 do not work because 2the changes are so slow that days or weeksof incubation are needed. If the microbes are concentrated by filtration or even placed in a bottle for more than a few hours then communities and rates change. “Richard Wright and I, young postdoctoral fellows at Uppsal ...
Building Monomers of Macromolecules
... REMEMBER: Molecules are 3-dimensional so models will not always be flat! Background info: Living cells are made up of mostly water. The rest of the cell is largely made up of compounds that contain Carbon. Living things are made up of proteins, DNA, fats, and sugars. All of these molecules are made ...
... REMEMBER: Molecules are 3-dimensional so models will not always be flat! Background info: Living cells are made up of mostly water. The rest of the cell is largely made up of compounds that contain Carbon. Living things are made up of proteins, DNA, fats, and sugars. All of these molecules are made ...
Genetics Lab Cystic Fibrosis Cystic fibrosis is a serious genetic
... Sometimes errors occur during the division process that result in cells with an additional chromosome or a deleted chromosome. Usually gametes with an unusual number of chromosomes simply do not have the opportunity to become an embryo. Although meiosis errors may occur in sperm cells, the greater n ...
... Sometimes errors occur during the division process that result in cells with an additional chromosome or a deleted chromosome. Usually gametes with an unusual number of chromosomes simply do not have the opportunity to become an embryo. Although meiosis errors may occur in sperm cells, the greater n ...
Chapter 6: An Introduction to Proteins
... hemoglobin will reach nearly 100% saturation with oxygen while myoglobin will remain essentially as deoxymyoglobin. ...
... hemoglobin will reach nearly 100% saturation with oxygen while myoglobin will remain essentially as deoxymyoglobin. ...
The exocyst, an octameric protein complex conserved among all
... of exocyst in exocytosis, new studies from both mammalian and plant fields report its involvement in the cellular self-eating process called autophagy. In land plants the number of paralogs of some exocyst subunits is extraordinarily large. There are 23 paralogs of Exo70 subunit in Arabidopsis thali ...
... of exocyst in exocytosis, new studies from both mammalian and plant fields report its involvement in the cellular self-eating process called autophagy. In land plants the number of paralogs of some exocyst subunits is extraordinarily large. There are 23 paralogs of Exo70 subunit in Arabidopsis thali ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.