1337 Biochemical properties of plasminogen activator inhibitor
... 50% (18, 24). After cleavage, the amino acid sequence from the N-terminus is either VHHPP- or SAVHHPP-. The correct numbering of the amino acids is then S1-A2-etc, but most workers in the field have chosen the numbering V1H2-etc. In this review the S1-A2-etc.-numbering will be used (18) with the ser ...
... 50% (18, 24). After cleavage, the amino acid sequence from the N-terminus is either VHHPP- or SAVHHPP-. The correct numbering of the amino acids is then S1-A2-etc, but most workers in the field have chosen the numbering V1H2-etc. In this review the S1-A2-etc.-numbering will be used (18) with the ser ...
Multiple sequence alignment for phylogenetic purposes
... which the protein structures are known (Fig. 1). The average pairwise amino-acid identity among the six full sequences is 21%, which is within what is known as the ‘twilight zone’ of protein similarity, where sequence alignment can be very problematic. The two most different sequences, human hydroxy ...
... which the protein structures are known (Fig. 1). The average pairwise amino-acid identity among the six full sequences is 21%, which is within what is known as the ‘twilight zone’ of protein similarity, where sequence alignment can be very problematic. The two most different sequences, human hydroxy ...
On the role and formation of covalently bound flavin cofactors Heuts
... cofactor (FMN or FAD), a large number have these cofactors covalently linked to the polypeptide chain. Most covalent flavin–protein linkages involve a single cofactor attachment via a histidyl, tyrosyl, cysteinyl or threonyl linkage. However, some flavoproteins contain a flavin that is tethered to two ...
... cofactor (FMN or FAD), a large number have these cofactors covalently linked to the polypeptide chain. Most covalent flavin–protein linkages involve a single cofactor attachment via a histidyl, tyrosyl, cysteinyl or threonyl linkage. However, some flavoproteins contain a flavin that is tethered to two ...
The Origin and Evolution of the Genetic Code
... already been published, and already incorporate some of my own research, I take this opportunity to give both an overview of the field as it was when I started work on this topic in 1997 and to outline what can be found in each of the review chapters. Excitement in genetic code research began with c ...
... already been published, and already incorporate some of my own research, I take this opportunity to give both an overview of the field as it was when I started work on this topic in 1997 and to outline what can be found in each of the review chapters. Excitement in genetic code research began with c ...
CHAPTER 1 INTRODUCTION
... them as a hay crop. It was first cultivated in South Africa in 1903 at Cedara in Natal (Cronje , 1997). It was not until after 1945 that their value as a supplier of feed and food oil was recognised and exploited (Snyder and Kwon , 1987). In South Africa a genetically improved soya bean with increas ...
... them as a hay crop. It was first cultivated in South Africa in 1903 at Cedara in Natal (Cronje , 1997). It was not until after 1945 that their value as a supplier of feed and food oil was recognised and exploited (Snyder and Kwon , 1987). In South Africa a genetically improved soya bean with increas ...
Systematic metabolic analysis of recombinant Pichia pastoris UNIVERSITAT AUTÒNOMA DE BARCELONA
... protein secretion on central metabolism, a first quantitative metabolomic analysis of free amino acids pools in a recombinant P. pastoris strain growing under different oxygen availability conditions was carried out. The obtained values indicated significant variations in the intracellular amino aci ...
... protein secretion on central metabolism, a first quantitative metabolomic analysis of free amino acids pools in a recombinant P. pastoris strain growing under different oxygen availability conditions was carried out. The obtained values indicated significant variations in the intracellular amino aci ...
ASD v2.0: updated content and novel features
... In addition to the largely expanded data volume, the search engine and browser functions are enhanced, and a number of new cross-links to related databases have been added. The data architecture and content of the download files have been redesigned for users to facilitate the analysis of the alloste ...
... In addition to the largely expanded data volume, the search engine and browser functions are enhanced, and a number of new cross-links to related databases have been added. The data architecture and content of the download files have been redesigned for users to facilitate the analysis of the alloste ...
Investigation of Factors Affecting Opalescence and Phase
... antibody-based therapeutics such as ADCs, bispecific antibodies, engineered antibodies and antibody fragments or domains have generated quite an interest over last few years. These newer molecules have further enhanced the therapeutic efficacy of the first generation of antibodies and have vastly im ...
... antibody-based therapeutics such as ADCs, bispecific antibodies, engineered antibodies and antibody fragments or domains have generated quite an interest over last few years. These newer molecules have further enhanced the therapeutic efficacy of the first generation of antibodies and have vastly im ...
Fast evolutionary rates associated with functional loss in class I
... to glycolysis in the presence of glucose immediately after invading the human host. This metabolic switch is dependent on extracellular glucose concentration. Four glucose transporters are encoded in the genome of S. mansoni, however, only two were shown to facilitate glucose diffusion. Results: By ...
... to glycolysis in the presence of glucose immediately after invading the human host. This metabolic switch is dependent on extracellular glucose concentration. Four glucose transporters are encoded in the genome of S. mansoni, however, only two were shown to facilitate glucose diffusion. Results: By ...
SCHEMA Recombination of a
... movement in the portion of the protein backbone, which positions Trp-253 and the intrinsic flexibility of Met side chains. Notably, one characterized chimera fits this pattern (13333232) and is more stable than the parents (67 °C), in accord with the regression model fit (68 °C). Another mechanism b ...
... movement in the portion of the protein backbone, which positions Trp-253 and the intrinsic flexibility of Met side chains. Notably, one characterized chimera fits this pattern (13333232) and is more stable than the parents (67 °C), in accord with the regression model fit (68 °C). Another mechanism b ...
Sequence and Structure Classification of Kinases
... ATP-grasp family. An evolutionary link between these three families based on structural similarity has been described.6 In each of the group 1 families, the active site of the enzyme is located in the cleft between two a þ b domains (Figure 1(a)). The protein kinase-like family members and SAICAR sy ...
... ATP-grasp family. An evolutionary link between these three families based on structural similarity has been described.6 In each of the group 1 families, the active site of the enzyme is located in the cleft between two a þ b domains (Figure 1(a)). The protein kinase-like family members and SAICAR sy ...
View PDF - Genes | Genomes | Genetics
... age of the two samples being compared. If SHH protein levels were identical in the left and right fore- or hindlimbs within an embryo at all stages of development, this would suggest that SHH levels do not deviate bilaterally during limb development. In contrast, if SHH protein levels were found to ...
... age of the two samples being compared. If SHH protein levels were identical in the left and right fore- or hindlimbs within an embryo at all stages of development, this would suggest that SHH levels do not deviate bilaterally during limb development. In contrast, if SHH protein levels were found to ...
The Structure of Collagen within Parchment
... Collagen molecules have the prerequisite of repeating arnino acid sequences of Gly-X-Y, where Gly is the amino acid residue glycine and X and Y are any amino acid residues; most commonly X and Y are proline and hydroxyproline34. Hy-droxylysine is another residue that commonly takes the X or Y posit ...
... Collagen molecules have the prerequisite of repeating arnino acid sequences of Gly-X-Y, where Gly is the amino acid residue glycine and X and Y are any amino acid residues; most commonly X and Y are proline and hydroxyproline34. Hy-droxylysine is another residue that commonly takes the X or Y posit ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
Endoplasmic reticulum localization of the low density lipoprotein
... cause hypercholesterolemia because of inefficient LDL clearance from the circulation. In addition, there is a paradoxical oversecretion of the metabolic precursor of LDL, very low density lipoprotein (VLDL). We recently demonstrated that the LDLR mediates presecretory degradation of the major VLDL p ...
... cause hypercholesterolemia because of inefficient LDL clearance from the circulation. In addition, there is a paradoxical oversecretion of the metabolic precursor of LDL, very low density lipoprotein (VLDL). We recently demonstrated that the LDLR mediates presecretory degradation of the major VLDL p ...
Technical Brief
... The term kwashiorkor was introduced in the medical literature by Cicely Williams, a Jamaican physician working in what is now Ghana in an article published in the Lancet in 1935 1 (republished in 20032). It is derived from a word of the Krobo language from Ghana and refers to a child displaced from ...
... The term kwashiorkor was introduced in the medical literature by Cicely Williams, a Jamaican physician working in what is now Ghana in an article published in the Lancet in 1935 1 (republished in 20032). It is derived from a word of the Krobo language from Ghana and refers to a child displaced from ...
H. Draw a dipeptide from two amino acids
... compound and a brief statement as to why you think your answer is reasonable. 17. In general, what are the potential problems associated with a strict vegetarian diet? 18. How many ketone structures can be drawn that are isomeric with the aldehyde pentanal? 19. Aldehydes and ketones have lower boili ...
... compound and a brief statement as to why you think your answer is reasonable. 17. In general, what are the potential problems associated with a strict vegetarian diet? 18. How many ketone structures can be drawn that are isomeric with the aldehyde pentanal? 19. Aldehydes and ketones have lower boili ...
post- translational modifications
... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
Barley Aleurone Cells Contain Two Types of
... which we have called secondary vacuoles. Differential interference contrast (DIC) microscopy showed that these two types of vacuole occur in both GA- and ABA-treated aleurone protoplasts (Figure 1). In Figure 1, the plane of focus was selected to most clearly reveal the characteristics of the second ...
... which we have called secondary vacuoles. Differential interference contrast (DIC) microscopy showed that these two types of vacuole occur in both GA- and ABA-treated aleurone protoplasts (Figure 1). In Figure 1, the plane of focus was selected to most clearly reveal the characteristics of the second ...
C. jejuni
... ATP-binding cassette transporters (ABC-transporter) are members of a protein superfamily that is one of the largest and most ancient families with representatives in all extant phyla from prokaryotes to humans. ABC transporters are transmembrane proteins that utilize the energy of ATP hydrolysis to ...
... ATP-binding cassette transporters (ABC-transporter) are members of a protein superfamily that is one of the largest and most ancient families with representatives in all extant phyla from prokaryotes to humans. ABC transporters are transmembrane proteins that utilize the energy of ATP hydrolysis to ...
Ribosomal Stalk Protein L12: Structure, Function and
... globular, highly conserved and provides sites for interaction with the GTPase factors (Helgstrand, Mandava et al. 2007). This compact domain consists of 3 α-helices and 3-β sheets spanning the residues 50-120. The N and C-terminal domains are connected by the hinge region which includes the residues ...
... globular, highly conserved and provides sites for interaction with the GTPase factors (Helgstrand, Mandava et al. 2007). This compact domain consists of 3 α-helices and 3-β sheets spanning the residues 50-120. The N and C-terminal domains are connected by the hinge region which includes the residues ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.