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General principles of cellular organization in the genome
General principles of cellular organization in the genome

... pneumoniae, which is among the smallest self-replicating forms of life on earth. My work hereby focuses on the analysis of the proteome organization, the part, among the three investigations, to which I contributed most substantially. In order to study basic principles of bacterial proteome organiza ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
Reconstitution of an Allophycocyanin Trimer Complex Containing

The C-terminal domain of the Rhizobium leguminosarum
The C-terminal domain of the Rhizobium leguminosarum

... pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod gene expression and the chromogenic indicator 5'-bromo-4'- ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
Queuosine formation in eukaryotic tRNA occurs via a mitochondria

... In contrast to the eubacterial enzyme—a single protein species—purification of the eukaryotic TGT suggested that the catalytically active enzyme is a heterodimeric molecule: subunits of 60 kDa and 43 kDa in rabbit erythrocytes (16); 66 kDa and 32 kDa in bovine liver (17), 60 kDa and 34.5 kDa in rat ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA

... A novel compound, the fatty acid synthase (FAS) inhibitor C75, has been proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained ...
2.2. Garrido-Franco, M. Structure E. coli
2.2. Garrido-Franco, M. Structure E. coli

... and C and exhibited high disorder factors in monomer D (average B value = 63.8 Å2). However, the backbone and most of the side chain atoms of loop 4 show continuos and unambiguous electron density in monomer D. The PNP synthase octamer is organized as a tetramer of dimers AE, BF, CG and DH. The (β/α ...
Bacterial protein toxins targeting Rho GTPases
Bacterial protein toxins targeting Rho GTPases

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Identification and analysis of new phloem proteins from

... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
METABOLIC CUES AND REGULATORY PROTEINS
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213 porters and neurotransmitter transporters
213 porters and neurotransmitter transporters

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Selenocysteine insertion directed by the 3′

Advances in Chemical Protein Modification
Advances in Chemical Protein Modification

PDF Full-text
PDF Full-text

Antimicrobial peptides in crustaceans
Antimicrobial peptides in crustaceans

... activities. Interestingly, an important number of gene-encoded AMPs in crustaceans are composed of different structural domains. Each of these domains possess singular features found in other AMP groups, such as the overrepresentation of specific amino acids or the presence of cysteine residues that ...
Ph.D. Thesis Azar Shahpiri
Ph.D. Thesis Azar Shahpiri

Development of prokaryotic cell-free systems for synthetic
Development of prokaryotic cell-free systems for synthetic

... Cell-free systems have historically been a fundamental tool for biological research. This was partially a necessity, as transformation technologies to introduce DNA into E. coli (Mandel & Higa, 1970) and modern recombinant DNA technology (Smith & Welcox, 1970) were not available until 1970. As a res ...
endoglucanase in cellulose biosynthesis is not very clear
endoglucanase in cellulose biosynthesis is not very clear

CO2 and O2 Distribution in Rubisco Suggests the
CO2 and O2 Distribution in Rubisco Suggests the

New Generation Collagens
New Generation Collagens

... We use the plural form of the word collagen, because ultimately we intend to produce many more products. We are able to do this because the fish collagen hydrate, in the form of a temperature resistant gel, makes it possible to emulsify with many other biologically active substances that are benefic ...
C H A P
C H A P

... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
A1018 High oleic GM soybean AppR SD1 Safety Assess AMENDED
A1018 High oleic GM soybean AppR SD1 Safety Assess AMENDED

... present at junctions associated with two of the insertion sites; neither of these ORFs is likely to be expressed due to the absence of the necessary regulatory sequences. Characterisation of Novel Protein The introduced gm-fad2-1 element is a partial sequence rather than a complete gene, therefore n ...
The contribution of the Trp/Met/Phe residues to physical interactions
The contribution of the Trp/Met/Phe residues to physical interactions

Alternative mRNA Splicing Generates the Two
Alternative mRNA Splicing Generates the Two

... alternative splicing of a common pre-mRNA. In spinach, two 5’ splice sites are used in processing a single 137nucleotide intron near the 3‘ end of the primary transcript. This intron was either removed completely or, alternatively, the first 22 nucleotides of the intervening sequence were retained i ...
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain

Functional genomics analysis of the effects of co- decarboxylase/ornithine decarboxylase
Functional genomics analysis of the effects of co- decarboxylase/ornithine decarboxylase

... parasites and normal progression of the untreated controls require special consideration for basic comparisons of response in terms of the assay methodology used and data analysis. This is particularly important when studying a multistage organism such as P. falciparum, which constantly develops and ...
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Protein structure prediction



Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.
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