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Lecture 21 Student Powerpoint
Lecture 21 Student Powerpoint

... a. Usually 20–25 bases in length b. 10–20 different oligonucleotides for each gene 2. Oligonucleotides for each gene selected by computer program to be the following: a. Unique in genome b. Nonoverlapping 3. Composition based on design rules a. Empirically derived ...
DNA Review Worksheet
DNA Review Worksheet

... a specific amino acid; the tRNA anticodon will pair up with its complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a peptide bond. As process continues, amino acid chain is formed until a stop codon. 4. The tRNA is recycled to find another of the ...
App1PCR - FSU Biology
App1PCR - FSU Biology

... to yield microgram quantities for subsequent biochemical analysis. In order to perform PCR, the DNA sequence flanking both sides of the target must be known. (Note that PCR cannot be used as a substitute for cloning unknown genes!) This information is used to synthesize short oligonucleotides (singl ...
Recombinant DNA Technology
Recombinant DNA Technology

... • DNA library = collection of clones from one DNA donor – Categorized by: vector, source of DNA – Genomic library = all of the sequences from the genome of a single organism – cDNA library= complementary DNA, made using mRNA as a template ...
6.5 Main Group
6.5 Main Group

... geological applications, e.g. this one … … was used here ...
3.13 Amino acids, proteins and DNA
3.13 Amino acids, proteins and DNA

Genetic Engineering - Biology Class With Mrs. Caskey
Genetic Engineering - Biology Class With Mrs. Caskey

... the cats’ DNA which causes them to glow in the dark. • Normally, the gene is found in ...
Specimen Collection for Quantitative PCR Assays
Specimen Collection for Quantitative PCR Assays

Guided Notes
Guided Notes

... DNA fingerprinting is used for identification.  DNA fingerprinting depends on the probability of a match.  Many people have the same number of repeats in a certain region of DNA  The probability that two people share identical numbers of repeats in several locations is ____ ________ (only one cha ...
An Introduction to DNA and Genetics Directions: As you watch the
An Introduction to DNA and Genetics Directions: As you watch the

... ________________________. Mutation causes different versions (alleles) of the same ____________. Parent ______________ are shuffled – or recombined – when sex cells created in the body. Because of _______________________, sexual reproduction produces more ...
Effects of mutations
Effects of mutations

... • Similar to procaryotes except – AUG encodes for a different form of methionine – Transcription and translation are not simultaneous (since eucaryotes have a nucleus----transcription occurs in the nucleus, translation occurs ?) – Eucaryotes must splice out introns to achieve a mature mRNA strand re ...
Café DNA - www .alexandria .k12 .mn .us
Café DNA - www .alexandria .k12 .mn .us

... Essential Question 3: How does DNA code for proteins and what does it have to do with how my body works? ...
From Genetic Code to Protein Structure Worksheet
From Genetic Code to Protein Structure Worksheet

... DNA is a polymer made of millions of sequences of just four nucleotides: A, T, C and G. These nucleotides serve as letters in the genetic code of life, dictating which of the 20 amino acids should be placed at specific places in the growing protein chain. One by one, the nucleotides cannot code 20 a ...
I. DNA, Chromosomes, Chromatin, and Genes II. DNA
I. DNA, Chromosomes, Chromatin, and Genes II. DNA

... a specific amino acid; the tRNA anticodon will pair up with its complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a peptide bond. As process continues, amino acid chain is formed until a stop codon. 4. The tRNA is recycled to find another of the ...
Molecules of Life
Molecules of Life

... • Two chains of nucleotides twisted together into a double helix and held by hydrogen bonds • Contains all inherited information necessary to build an organism, coded in the order of nucleotide bases ...
HB Final Exam Review Guide
HB Final Exam Review Guide

GENE EXPRESSION CHAPTER 11
GENE EXPRESSION CHAPTER 11

... known commonly for their illegal use by athletes, anabolic steroids are used medically to treat growth abnormalities, anemia, leukemia, kidney failure, and other medical problems. ...
Document
Document

... • Two chains of nucleotides twisted together into a double helix and held by hydrogen bonds • Contains all inherited information necessary to build an organism, coded in the order of nucleotide bases ...
Biological Molecules
Biological Molecules

... put amino acids together in a particular order to make a particular protein. As long as the DNA contains the correct code, the protein will function. Mistakes in the code (mutations) change the order of amino acids, which changes the structure of the protein, which prevents the protein from carrying ...
Genes Are DNA
Genes Are DNA

... Nitrogenous Bases 鹼基Linked to a Sugar–Phosphate Backbone A nucleoside consists of a purine or pyrimidine base linked to position 1 of a pentose sugar. ...
BIOL10005: Genetics and the Evolution of Life
BIOL10005: Genetics and the Evolution of Life

... The method of DNA replication where the new molecule of DNA has one strand which comes from the parent molecule and one strand which is newly synthesised Nucleotides or nucleotide sequences that are able to base pair, for example G and C are complementary, as are A and T One of the two types of nitr ...
Cell Cycle, DNA, and Protein Synthesis
Cell Cycle, DNA, and Protein Synthesis

... During mitosis, _________ parent cell divides into _________ identical daughter cells. All _______________ cells (cells other than the sex cells that make eggs and sperm) ...
HASPI Medical Biology Lab 07a Background
HASPI Medical Biology Lab 07a Background

... meaning they have some double-bonds and still have some space available for hydrogen atoms to bond. Fatty acids can be bonded to other molecules such as glycerol and phosphates to form lipids. Examples of lipids include triglycerides and phospholipids. Monomer Fatty Acid ...
DNA to RNA
DNA to RNA

... Clelland et al. Nature 399:533. Hiding messages in DNA microdots. ...
Lab Techniques
Lab Techniques

... • Allow specific cutting and removal of genes from a complex molecule of DNA. • Complementary sticky ends (cohesive ends) allow joining of DNA molecules. "The work on restriction nucleases not only permits us easily to construct recombinant DNA Molecules and to analyze individual genes but also has ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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