HiPer® Plasmid DNA Cloning Teaching Kit
... two DNA fragments (having blunt or overhanging, complementary, 'sticky' ends) by the help of an enzyme named as ligase. DNA ligase forms a phosphodiester bond between the 3’ hydroxyl of one nucleotide and the 5’ phosphate of another. Ligation can be directional or non-directional based upon the rest ...
... two DNA fragments (having blunt or overhanging, complementary, 'sticky' ends) by the help of an enzyme named as ligase. DNA ligase forms a phosphodiester bond between the 3’ hydroxyl of one nucleotide and the 5’ phosphate of another. Ligation can be directional or non-directional based upon the rest ...
NABP1, a novel RORγ-regulated gene encoding a single
... full-length NABP1 and NABP1N (amino acids 146–198) respectively, into the EcoRI and BamHI sites of pMAL-C2X (New England BioLabs, Ipswich, MA, U.S.A.). NABP1 was amplified with the sense, 5 -GAGAATTCATGCACGGGGTCAACGAC-3 , and antisense, 5 -GTGGATCCTCATCATTTAAAGGCTCTCCTC-3 , primers, NABP1N was ...
... full-length NABP1 and NABP1N (amino acids 146–198) respectively, into the EcoRI and BamHI sites of pMAL-C2X (New England BioLabs, Ipswich, MA, U.S.A.). NABP1 was amplified with the sense, 5 -GAGAATTCATGCACGGGGTCAACGAC-3 , and antisense, 5 -GTGGATCCTCATCATTTAAAGGCTCTCCTC-3 , primers, NABP1N was ...
Close relationship between non-viral retroposons in Drosophila
... Cx 2 Cx 4 Hx 4 C motif, and two adjacent imperfect ones (fig. 7a) are found within G-orfl, a 241 amino acid long orf that partially overlaps G-orf2 (figs. 2 and 3). In fig. 7b the G-orfl region encompassing the cysteine-rich motifs is aligned with the homologous regions in F-orf 1 and Iorfl. The hom ...
... Cx 2 Cx 4 Hx 4 C motif, and two adjacent imperfect ones (fig. 7a) are found within G-orfl, a 241 amino acid long orf that partially overlaps G-orf2 (figs. 2 and 3). In fig. 7b the G-orfl region encompassing the cysteine-rich motifs is aligned with the homologous regions in F-orf 1 and Iorfl. The hom ...
Slide 1
... • Proteins contain the elements C H O N & sometimes S • They are made by condensation reactions between amino acids forming long polypeptide chains. • The properties of each individual protein are determined by the aa sequence ...
... • Proteins contain the elements C H O N & sometimes S • They are made by condensation reactions between amino acids forming long polypeptide chains. • The properties of each individual protein are determined by the aa sequence ...
05 - summer quiz 2011.tst
... B) the peptide bonds linking amino acids differ from protein to protein. C) the sequence of amino acids in the polypeptide chain differs from protein to protein. D) each protein contains its own unique sequence of sugar molecules. E) the number of nucleotides found in each protein varies from molecu ...
... B) the peptide bonds linking amino acids differ from protein to protein. C) the sequence of amino acids in the polypeptide chain differs from protein to protein. D) each protein contains its own unique sequence of sugar molecules. E) the number of nucleotides found in each protein varies from molecu ...
Biology 6 Test 2 Study Guide
... iii. Ionizing radiation – rays will ionize normal compounds and make them react inappropriately with other molecules. E.g. form covalent bonds. iv. Ultra violet light (UV) – forms T-T dimers. These stall replication c. Repair i. Repair mechanisms exist to fix mistakes (Fig. 8.21) ii. DNA polymerase ...
... iii. Ionizing radiation – rays will ionize normal compounds and make them react inappropriately with other molecules. E.g. form covalent bonds. iv. Ultra violet light (UV) – forms T-T dimers. These stall replication c. Repair i. Repair mechanisms exist to fix mistakes (Fig. 8.21) ii. DNA polymerase ...
Chapter 6 – Microbial Growth
... iii. Ionizing radiation – rays will ionize normal compounds and make them react inappropriately with other molecules. E.g. form covalent bonds. iv. Ultra violet light (UV) – forms T-T dimers. These stall replication c. Repair i. Repair mechanisms exist to fix mistakes (Fig. 8.21) ii. DNA polymerase ...
... iii. Ionizing radiation – rays will ionize normal compounds and make them react inappropriately with other molecules. E.g. form covalent bonds. iv. Ultra violet light (UV) – forms T-T dimers. These stall replication c. Repair i. Repair mechanisms exist to fix mistakes (Fig. 8.21) ii. DNA polymerase ...
the code of translation
... 4. A peptide bond forms between the first two amino acids. 5. The first tRNA leaves, and the ribosome moves along the mRNA to the next codon. 6. The next tRNA brings in the next amino acid, and a peptide bond is formed between this amino acid and the growing amino acid chain. 7. The process continu ...
... 4. A peptide bond forms between the first two amino acids. 5. The first tRNA leaves, and the ribosome moves along the mRNA to the next codon. 6. The next tRNA brings in the next amino acid, and a peptide bond is formed between this amino acid and the growing amino acid chain. 7. The process continu ...
Transcription from DNA Virus Genomes
... • Pol I - pre rRNA not known to be used by viruses • Pol II - makes mRNAs and some micro RNAs • Pol III - Adenovirus VA RNAs, EBV EBERs and some micro RNAs ...
... • Pol I - pre rRNA not known to be used by viruses • Pol II - makes mRNAs and some micro RNAs • Pol III - Adenovirus VA RNAs, EBV EBERs and some micro RNAs ...
Genes can encode proteins or non
... retrotransposon) and reinserted into the genome. This process is considered non-conservative, since each round makes a new DNA element. Excisions of transposons can be catalyzed by some transposases. These can be identified by loss of an associated marker. Often excision is imprecise and results in ...
... retrotransposon) and reinserted into the genome. This process is considered non-conservative, since each round makes a new DNA element. Excisions of transposons can be catalyzed by some transposases. These can be identified by loss of an associated marker. Often excision is imprecise and results in ...
Genes can encode proteins or non
... retrotransposon) and reinserted into the genome. This process is considered non-conservative, since each round makes a new DNA element. Excisions of transposons can be catalyzed by some transposases. These can be identified by loss of an associated marker. Often excision is imprecise and results in ...
... retrotransposon) and reinserted into the genome. This process is considered non-conservative, since each round makes a new DNA element. Excisions of transposons can be catalyzed by some transposases. These can be identified by loss of an associated marker. Often excision is imprecise and results in ...
Macromolecules Webquest
... and answer the following questions. If you miss something in the movie, the script is written out below. If you are unsure about some of the information, use the internet to find more info. 1. What did William Prout study? Why did he study this? ...
... and answer the following questions. If you miss something in the movie, the script is written out below. If you are unsure about some of the information, use the internet to find more info. 1. What did William Prout study? Why did he study this? ...
SP7+ P7 (1+3) Energetics and kinetics of chemical reaction.
... points. Scores in four partial written exams (C1, C2, B1, B2) and a comprehensive final exam (practicals) are the principal means of accumulating points. Students who attended lectures and practicals could write partial written exams, while final exam from practicals is prerequisite for attending B2 ...
... points. Scores in four partial written exams (C1, C2, B1, B2) and a comprehensive final exam (practicals) are the principal means of accumulating points. Students who attended lectures and practicals could write partial written exams, while final exam from practicals is prerequisite for attending B2 ...
recombinant dna
... categorize in two groups based on the types of cuts they make. The first type, As exemplified by EcoRI, ...
... categorize in two groups based on the types of cuts they make. The first type, As exemplified by EcoRI, ...
Chapter 5 - Trimble County Schools
... helix Hydrogen bond pleated sheet strand Hydrogen bond ...
... helix Hydrogen bond pleated sheet strand Hydrogen bond ...
Biochemistry Review Test
... 30. A hydrophobic amino acid R group would be found where in a protein? (1.) forming a peptide bond with the next amino acid in the chain (2.) on the outside of the folded chain, in the water (3.) on the inside of the folded chain, away from water (4.) forming hydrogen bonds with other R groups (5. ...
... 30. A hydrophobic amino acid R group would be found where in a protein? (1.) forming a peptide bond with the next amino acid in the chain (2.) on the outside of the folded chain, in the water (3.) on the inside of the folded chain, away from water (4.) forming hydrogen bonds with other R groups (5. ...
BDOL Interactive Chalkboard
... the DNA, making millions of copies in less than a day. • Because the machine uses heat to separate the DNA strands and cycles over and over to replicate the DNA, it is called a thermocycler. ...
... the DNA, making millions of copies in less than a day. • Because the machine uses heat to separate the DNA strands and cycles over and over to replicate the DNA, it is called a thermocycler. ...
ch_02 - HCC Learning Web
... cells and viruses. These differ primarily in the structure of their monomers, which are called nucleotides. Each nucleotide consists of phosphate, a pentose sugar (deoxyribose or ribose), and one of five cyclic nitrogenous bases: adenine (A), guanine (G), cytosine (C), thymine (T), uracil (U). DNA c ...
... cells and viruses. These differ primarily in the structure of their monomers, which are called nucleotides. Each nucleotide consists of phosphate, a pentose sugar (deoxyribose or ribose), and one of five cyclic nitrogenous bases: adenine (A), guanine (G), cytosine (C), thymine (T), uracil (U). DNA c ...
Standard 4 study guide
... This animal inhabits trees and is only able to live in warm, humid environments. Due to its slow movement (and the presence of warmth and moisture), a green mold will often grow on the fur of the sloth, helping it to blend in or camouflage with its surroundings. If the rainforest this organism occup ...
... This animal inhabits trees and is only able to live in warm, humid environments. Due to its slow movement (and the presence of warmth and moisture), a green mold will often grow on the fur of the sloth, helping it to blend in or camouflage with its surroundings. If the rainforest this organism occup ...
What is the difference between allele, gene, and trait?
... Failure of homologous to separate during Anaphase I (resulting in four affected daughter cells) Failure of sister chromatids to separate during Anaphase II (resulting in two affected daughter cells) One parental gamete is normal and has a single copy of chromosome 21 but the other parental gamet ...
... Failure of homologous to separate during Anaphase I (resulting in four affected daughter cells) Failure of sister chromatids to separate during Anaphase II (resulting in two affected daughter cells) One parental gamete is normal and has a single copy of chromosome 21 but the other parental gamet ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.