Chapter 26:Biomolecules: Amino Acids, Peptides, and Proteins
... • Only the pyridine-like, doubly bonded nitrogen in histidine is basic. The pyrrole-like singly bonded nitrogen is nonbasic because its lone pair of electrons is part of the 6 electron aromatic imidazole ring (see Section 24.4). ...
... • Only the pyridine-like, doubly bonded nitrogen in histidine is basic. The pyrrole-like singly bonded nitrogen is nonbasic because its lone pair of electrons is part of the 6 electron aromatic imidazole ring (see Section 24.4). ...
AMINO ACIDS, POLYPEPTIDES, AND PROTEINS
... Thin layer chromatography is a way of separating substances based on a differential distribution between two phases, the liquid phase and the solid phase. A strip (or sheet) is prepared with a thin coating (layer) of dried alumina or other adsorbent. A tiny spot of solution containing a mixture of a ...
... Thin layer chromatography is a way of separating substances based on a differential distribution between two phases, the liquid phase and the solid phase. A strip (or sheet) is prepared with a thin coating (layer) of dried alumina or other adsorbent. A tiny spot of solution containing a mixture of a ...
Solution structure of the Drosha double-stranded RNA-binding domain Open Access
... with the RNA, adding a new substrate recognition feature. However, the loop is negatively charged, and although this does not exclude nucleic acid interaction ...
... with the RNA, adding a new substrate recognition feature. However, the loop is negatively charged, and although this does not exclude nucleic acid interaction ...
This exam has 9 pages, including this one.
... Part C: Detailed Calculations - You must attempt all three questions. However, you have two choices within each question. C1: Do one of the following two questions (6 pts). i) A protein that is 20 amino acid residues in length folds into a stable structure. Assume that the protein forms all but one ...
... Part C: Detailed Calculations - You must attempt all three questions. However, you have two choices within each question. C1: Do one of the following two questions (6 pts). i) A protein that is 20 amino acid residues in length folds into a stable structure. Assume that the protein forms all but one ...
metabolism of lipids
... Iron-high agar Agar turns black when H2S +, no color change when H2S – E.g. Salmonella sp; some Proteus sp. ...
... Iron-high agar Agar turns black when H2S +, no color change when H2S – E.g. Salmonella sp; some Proteus sp. ...
Similarity
... It is easy to score if an amino acid is identical to another (the score is 1 if identical and 0 if not). However, it is not easy to give a score for amino acids that are somewhat similar. ...
... It is easy to score if an amino acid is identical to another (the score is 1 if identical and 0 if not). However, it is not easy to give a score for amino acids that are somewhat similar. ...
This exam has 9 pages, including this one.
... a) Explain which buffer compound you would use and why. If you are uncertain of what to choose, just pick one and move on, either choice will be graded in the following sections (2 pts). b) Determine the correct ratio of the acidic and conjugate base form of the buffer.(5 pts) c) Explain how you wou ...
... a) Explain which buffer compound you would use and why. If you are uncertain of what to choose, just pick one and move on, either choice will be graded in the following sections (2 pts). b) Determine the correct ratio of the acidic and conjugate base form of the buffer.(5 pts) c) Explain how you wou ...
Epigenetic differences arise during the lifetime of
... as described in ref. 9. The nonmethylated sites were cut in an initial digestion using the methylation-sensitive endonuclease SmaI (Amersham Pharmacia Biotech, Buckinghamshire, U.K.), which leaves blunt ends. A second digestion was performed using the isoschizomer PspAI (Stratagene), which leaves a ...
... as described in ref. 9. The nonmethylated sites were cut in an initial digestion using the methylation-sensitive endonuclease SmaI (Amersham Pharmacia Biotech, Buckinghamshire, U.K.), which leaves blunt ends. A second digestion was performed using the isoschizomer PspAI (Stratagene), which leaves a ...
Chapter 7
... associations form in the context of two strands of nucleic acids, their spacing is also uniform and highly stable. You may recall that hydrogen bonds are relatively weak bonds. However, in the context of DNA, the hydrogen bonding is what makes DNA extremely stable and therefore well suited as a long ...
... associations form in the context of two strands of nucleic acids, their spacing is also uniform and highly stable. You may recall that hydrogen bonds are relatively weak bonds. However, in the context of DNA, the hydrogen bonding is what makes DNA extremely stable and therefore well suited as a long ...
File
... • bind to the active site of the enzyme and participate in catalysis but are not considered substrates of the reaction • function as intermediate carriers of electrons, specific atoms or functional groups that are transferred in the overall reaction • Examples: NAD, NADP, FAD, CoEnzymeA ...
... • bind to the active site of the enzyme and participate in catalysis but are not considered substrates of the reaction • function as intermediate carriers of electrons, specific atoms or functional groups that are transferred in the overall reaction • Examples: NAD, NADP, FAD, CoEnzymeA ...
biological activities of purine analogues
... ligands and regulate gene expression. These RNA elements are located most commonly within the non-coding regions of bacterial mRNAs, because these genes were involved in fundamental metabolic pathways in certain bacterial pathogens. Purine-binding riboswitches may be targets for the development of n ...
... ligands and regulate gene expression. These RNA elements are located most commonly within the non-coding regions of bacterial mRNAs, because these genes were involved in fundamental metabolic pathways in certain bacterial pathogens. Purine-binding riboswitches may be targets for the development of n ...
Hydrolysis of Aspartame
... butan-1ol:ethanoic acid:distilled water (prepared as instructions). This solvent is known as the eluent. Make sure the bottom of the paper is in the eluent but the spots are not. Cover the beaker with foil to prevent evaporation. Remove from the beaker when the solvent front is just at the top of th ...
... butan-1ol:ethanoic acid:distilled water (prepared as instructions). This solvent is known as the eluent. Make sure the bottom of the paper is in the eluent but the spots are not. Cover the beaker with foil to prevent evaporation. Remove from the beaker when the solvent front is just at the top of th ...
fulltext - DiVA Portal
... are inherited insofar as they are present in the germline cells but in other instances external influences from pathogens can cause disease for example via production of foreign proteins or, through harmful alterations of the regulations of the biological processes in our cells. As a result of our i ...
... are inherited insofar as they are present in the germline cells but in other instances external influences from pathogens can cause disease for example via production of foreign proteins or, through harmful alterations of the regulations of the biological processes in our cells. As a result of our i ...
Amino acid catabolism
... 2. Urea Cycle: Conversion of NH3 to urea for excretion 3. Metabolic break down of carbon skeleton to generate common intermediates that can be catabolized to CO2 or used in anabolic pathways to be stored as glucose or fat. ...
... 2. Urea Cycle: Conversion of NH3 to urea for excretion 3. Metabolic break down of carbon skeleton to generate common intermediates that can be catabolized to CO2 or used in anabolic pathways to be stored as glucose or fat. ...
paper - Lirmm
... graph in order to avoid the creation of paths that do not correspond to actual putative DNA sequences for the given protein. Also, as the scoring system we propose in this paper requires to differentiate identical symbols by their context, identical nucleotides appearing at the third position of dif ...
... graph in order to avoid the creation of paths that do not correspond to actual putative DNA sequences for the given protein. Also, as the scoring system we propose in this paper requires to differentiate identical symbols by their context, identical nucleotides appearing at the third position of dif ...
PHASE II--Conjugation Reactions A. Glucuronidation-
... b. stereoselectivity (although some nonenzymatic--most enzymatic) c. enzyme accounts for up to 10% of total cellular protein d. Binds stores, transports (heme, bilirubin, steroids, azo-dyes, PAHs) 6. Enzymatic substrates a. direct conjugation b. B/T by Phase I/II electrophile that is then conjugated ...
... b. stereoselectivity (although some nonenzymatic--most enzymatic) c. enzyme accounts for up to 10% of total cellular protein d. Binds stores, transports (heme, bilirubin, steroids, azo-dyes, PAHs) 6. Enzymatic substrates a. direct conjugation b. B/T by Phase I/II electrophile that is then conjugated ...
removal of amino gp from glutamate to release ammonia Other
... 3. Metabolic break down of carbon skeleton to generate common intermediates that can be catabolized to CO2 or used in anabolic pathways to be stored as glucose or fat. ...
... 3. Metabolic break down of carbon skeleton to generate common intermediates that can be catabolized to CO2 or used in anabolic pathways to be stored as glucose or fat. ...
Full-Text PDF
... acid interactions have been demonstrated for Arg, Ile, His, Phe, Tyr and Trp [15]. These results are consistent with those obtained by Johnson and Wong [14] in their ribosomal RNA-protein interaction study. These authors found that Gly, Ala, Val, Pro, Ser, Glu and Thr do not bind to RNA regions cont ...
... acid interactions have been demonstrated for Arg, Ile, His, Phe, Tyr and Trp [15]. These results are consistent with those obtained by Johnson and Wong [14] in their ribosomal RNA-protein interaction study. These authors found that Gly, Ala, Val, Pro, Ser, Glu and Thr do not bind to RNA regions cont ...
Identifying a Knockout Line from Seedpool
... 24. Carefully, pipet off the ethanol solution using a P1000 pipetman by placing the opening of the pipet tip against the bottom of the 14-mL tube so that ONLY ethanol is pipetted off. Note: if seeds are in the pipet tip, pipet the ethanol solution and seeds back to the tube. Then, pipet off the etha ...
... 24. Carefully, pipet off the ethanol solution using a P1000 pipetman by placing the opening of the pipet tip against the bottom of the 14-mL tube so that ONLY ethanol is pipetted off. Note: if seeds are in the pipet tip, pipet the ethanol solution and seeds back to the tube. Then, pipet off the etha ...
Chapter 17
... • Three properties of RNA enable it to function as an enzyme – It can form a three-dimensional structure because of its ability to base pair with itself – Some bases in RNA contain functional groups – RNA may hydrogen-bond with other nucleic acid molecules ...
... • Three properties of RNA enable it to function as an enzyme – It can form a three-dimensional structure because of its ability to base pair with itself – Some bases in RNA contain functional groups – RNA may hydrogen-bond with other nucleic acid molecules ...
4.Lect Carbon skeleton intro
... synthesize glucose and are termed glucogenic. while some are converted to acetylCoA (ketogenic amino acids) these CANNOT be used to synthesize glucose. Ketogenic amino acids can be converted to fatty acids for storage as triglyceride and later oxidation (fed state), or to ketone bodies (made in live ...
... synthesize glucose and are termed glucogenic. while some are converted to acetylCoA (ketogenic amino acids) these CANNOT be used to synthesize glucose. Ketogenic amino acids can be converted to fatty acids for storage as triglyceride and later oxidation (fed state), or to ketone bodies (made in live ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.