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Array CGH for detection of chromosome imbalance
Array CGH for detection of chromosome imbalance

... Array CGH analysis of DNA from XXX has been carried out using oligonucleotide arrays with ~44,000 probes across the genome. This test identified an apparently terminal deletion of approximately 84kb from band p16.3 in the short arm of chromosome 4, between base pair coordinates 72,446 and 156,159. T ...
pH - TeacherWeb
pH - TeacherWeb

... has a sulfhydryl (—SH) group in the R group, which suggests that this amino acid is likely to participate in intramolecular bonding. ...
Document
Document

... • Concentration of adenine (A) is equal to thymine (T) • Concentration of cytidine (C) is equal to guanine (G). • Watson-Crick base-pairing A will only base-pair with T, and C with G • base-pairs of G and C contain three H-bonds, • Base-pairs of A and T contain two H-bonds. • G-C base-pairs are more ...
methodology for high-quality RNA extraction from poultry whole
methodology for high-quality RNA extraction from poultry whole

Model Worksheet Student Handout
Model Worksheet Student Handout

... Model Worksheet Student Handout Introduction Despite the complexity of life on Earth, the most important large molecules found in all living things (biomolecules) can be classified into only four main categories: carbohydrates, lipids, proteins and nucleic acids. Three of these four classes of biomo ...
Learning Log/ FRQ-style Question
Learning Log/ FRQ-style Question

... LO 4.24: The Student is able to predict the effects of a change in an environmental factor on the genotypic expression of the phenotype. SP 6.4: The student can make claims and predictions about natural phenomena based on scientific theories and models. Explanation: An organism's ability to adapt t ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... • Extra nucleotides are removed from the 5’ends of pre-tRNA in one step by an endonucleolytic cleavage catalyzed by RNase P • RNase P from bacteria and eukaryotic nuclei have a catalytic RNA subunit called M1 RNA • Spinach chloroplast RNase P appears to lack an RNA subunit ...
Lipids (McMurry Ch. 27)
Lipids (McMurry Ch. 27)

... dipole-dipole forces and hydrogen bonding interactions. Amphiphilic = ...
УДК: 547
УДК: 547

... humans when compared with the use of multivariate analysis and mathematical modeling. At the ...
comparing dna sequences to determine evolutionary relationships
comparing dna sequences to determine evolutionary relationships

... >gi|377685879|gb|JN850779.1| Canis lupus familiaris isolate dog_3 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial ClustalX uses the letters after “>” until the first space as the label for the file. In this case, the label is “gi|377685879|gb|JN850779.1|” The label will be used o ...
Protocol
Protocol

... Avoid target sequences with significant homology to other genes, unless the shRNA is intended to knockdown a gene family. A target sequence within an open reading frame is preferred; targeting 3’ UTR is ok. No more than 4 consecutive T in the shRNA sequence to cause early transcription termination. ...
Next generation sequencing
Next generation sequencing

... the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively parallel sequencing generates 100 Mb to 1 Gb of short sequence reads in a single experime ...
Docking Studies in Target Proteins Involved in Antibacterial Action
Docking Studies in Target Proteins Involved in Antibacterial Action

... compounds scored lower than 1 μM. Regarding PBP1a, the best results were obtained with neogrifoline and 3,11-dioxolanosta-8,24(Z)-diene-26-oic acid; the latter also presented the highest score for Ddl. Otherwise, the score of the mentioned compound for Alr was low, being the highest score obtained w ...
Unit 4 Schedule
Unit 4 Schedule

... The instruction is copied or transcribed from the DNA in the nucleus. This copy is in the form of ribonucleic acid (RNA) in the form of messenger RNA (mRNA), which carries a copy of the genetic instruction from the nucleus to the ribosome. ...
No Slide Title
No Slide Title

... Glc 6-phosphate + 2NADP+ + H2O  ribose 5-phos + CO2 + 2NADPH + 2H+ ...
P - GMC Surat
P - GMC Surat

...  Require ATP.  Extreme specificity  High fidelity of translation of the genetic message.  Enzyme also have a “proofreading” or “editing” activity that can remove mischarged amino acids from the enzyme or the t-RNA molecule. ...
cDNA chips
cDNA chips

... Readout using micro-flow cells or optic fiber arrays ...
Yeast Transformation
Yeast Transformation

... Plate the transformed cells on selective media lacking uracil 7. Remove 10 µL of the resuspended cells to 90 µL of sterile water in a microcentrifuge tube. This sample will be serially diluted for a spot plate (step 10) that you will use to calculate the transformation efficiency. 8. Plate the ...
Agrobacterium-mediated DNA transfer, and then some
Agrobacterium-mediated DNA transfer, and then some

PowerPoint 演示文稿
PowerPoint 演示文稿

... mRNA has the same function in all cells, but there are important differences in the details of the synthesis and structure between prokaryotic and eukaryotic mRNA. ...
Two-Metal-Ion Catalysis in Adenylyl Cyclase
Two-Metal-Ion Catalysis in Adenylyl Cyclase

... tion, binds at site B. Mn2⫹ is an activator of AC, whereas Zn2⫹ is an inhibitor [IC50 ⫽ 15 ␮M (13)]. Although Zn2⫹ does not generally inhibit two-metal-ion– utilizing enzymes (16 –18), it does inhibit several DNA and RNA polymerases (19). In these enzymes and AC, Zn2⫹ may perturb the coordination of ...
Homework #1 BCHS 3304
Homework #1 BCHS 3304

... 8. Study exercises in FOB p 38 1, 3, 4, and 7. 9. Problems in FOB p 38 1, 3, 5, 7, 9, and 10. 10. Study Guide problems p17. 1, 3, 4, 6, 7, 8, 10, 11, 13, 15, and 17. 11. Proteins are made of amino acids. To calculate the molecular weight of a protein the mean residue weight is summed up for all cons ...
FREE Sample Here
FREE Sample Here

... B) charge-charge interactions between acidic and basic amino acids C) extensive hydrogen bonding due to the relatively high serine and glutamine content D) hydrophobic interactions E) an amide bond formed from the R-groups of a glutamic acid residue and a lysine residue Answer: A Difficulty: 1 ...
Gene mutation and DNA polymorphism
Gene mutation and DNA polymorphism

... The occurrence in a population of two or more genetically determined forms in such frequencies that the rarest of them could not be maintained by mutation alone A polymorphic locus is one at which there are at least two alleles, each with a frequency greater than 1%. Alleles with frequencies less th ...
Amino Acid Metabolism - Breakdown Other metabolic
Amino Acid Metabolism - Breakdown Other metabolic

... Glc 6-phosphate + 2NADP+ + H2O  ribose 5-phos + CO2 + 2NADPH + 2H+ ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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