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Are You suprised ?
Are You suprised ?

... in the amino-acid sequences of two organisms, the more closely related they are in an evolutionary sense. Conversely, the greater the time that organisms have been diverging from a common ancestor, the greater the differences that can be expected in the amino-acid sequences of their proteins. Two pr ...
Reading for Nitrogen Cycle Station
Reading for Nitrogen Cycle Station

... Ammonification - This is part of the decaying process. When a plant or animal dies, decomposers like fungi and bacteria turn the nitrogen back into ammonium so it can reenter the nitrogen cycle. Denitrification - Extra nitrogen in the soil gets put back out into the air. There are special bacteria t ...
Gel Electrophoresis
Gel Electrophoresis

... DNA fragments longer than about 20 kb cannot be resolved in conventional agarose gel electrophoresis because long DNA molecules align themselves as rods and migrate with a mobility that is independent of their length. In pulsed field gel electrophoresis (PFGE), the molecules are subjected to two alte ...
Docking QM/MM
Docking QM/MM

... improved H-bonding interaction overcomes the small distortion cost. Experimental evidence for this type of effect can be seen from both microwave studies of common substituents such as aniline60 and from an analysis of small molecule structures reported in the CSD where hydrogen atom positions have ...
Metabolism of [14C]glutamate and [14C]glutamine by
Metabolism of [14C]glutamate and [14C]glutamine by

... '"CO, release in detail, but some observations are worth noting. We found that, after 2 h incubation, 34% and 25% of '"C was lost as '"CO, during [14C]glutamate and [14C]glutamine feeding respectively. This accounted for approximately 80% of the difference between the total amount of absorbed radioa ...
characterization of proteins from the cytoskeleton of giardia lamblia
characterization of proteins from the cytoskeleton of giardia lamblia

... detected by PAGE Blue 83 dye-binding after focusing in a broad-range ampholyte gel. Giardin is slightly less acidic than tubulin. On gels it splits into four major and four minor chains with isoelectric points in the pi range from 5-8 to 6 - 2. The amino acid composition of the giardin fraction has ...
Zinc and the Body - Harlem Children Society
Zinc and the Body - Harlem Children Society

... • Zinc salts are known to shorten the duration and severity of the common cold. • Increasing the percentage of free zinc ions as compared to chelated zinc increases the efficacy of this treatment. • A study of the influence that amino acids have on the solubility of zinc citraconate may be relevant ...
Experiment 1 - Melting Points - NAU jan.ucc.nau.edu web server
Experiment 1 - Melting Points - NAU jan.ucc.nau.edu web server

... substantial amounts of another compound might result in a new melting point range from 85 to 100°C. An identical or near identical temperature range of melting is not, in itself, proof of the sameness of two organic chemical samples. There are thousands of solid organic compounds that melt within an ...
SpyTag and SpyCatcher - Department of Biochemistry
SpyTag and SpyCatcher - Department of Biochemistry

... encephalitis virus domain and then conjugated to antiDEC205-SpyTag to create a full vaccine. The modular vaccine generated efficient cytotoxic T-cell and antibody responses [34] and may overcome the frequent timeconsuming challenges in finding expression hosts and folding conditions compatible wit ...
Specific features of glycogen metabolism in the liver
Specific features of glycogen metabolism in the liver

... found in ŠiŠo. This paradoxical finding led them to invoke the existence of a still-unidentified stimulator of glycogen synthase or of an alternative pathway for glycogen synthesis. However, it is questionable whether the adopted effector concentrations are physiologically relevant, in particular in ...
Novel Ciliate Genetic Code Variants Including the Reassignment of
Novel Ciliate Genetic Code Variants Including the Reassignment of

... and is common to all ciliates. If the latter is true, it could explain the high propensity of ciliates to stop codon reassignment. The difference in genetic codes among ciliates would depend primarily on the availability of specific tRNAs for recognition of stop codons when those occur in internal p ...
Cholesterol And Sterol Metabolism
Cholesterol And Sterol Metabolism

... adenyl cyclase producing cAMP cAMP activates protein kinase A Inactivates HMG-CoA reductase ...
Support Material
Support Material

... contemporary areas of the subject. The syllabus also aims at emphasizing on the underlying principles that are common to both animals and plants as well as highlighting the realtionship of biology with other areas of knowledge.The format of the syllabus allows a simple, clear, sequential ¯ow of conc ...
odour away
odour away

... Bacillus (BPB), Lactic Acid Bacteria (LAB), Purple Non-Sulphur Bacteria (PNSB) and Yeast and their metabolites (enzymes, organic acids and organic metal chelates) specially formulated to neutralise odour. The ingredients used in the manufacture of Odour Away are all food grade. Odour Away is manufac ...
MOLES AND CALCULATIONS USING THE MOLE CONCEPT
MOLES AND CALCULATIONS USING THE MOLE CONCEPT

... 1. A mole is the amount of any substance that contains as many elementary entities as there are atoms in exactly 1.00 g of hydrogen-1. 2. A mole is the amount ... in exactly 12.00 g of carbon-12. 3. 6.02 x 1023 of anything 4. It is important to state the entities involved: atoms, molecules, ions, el ...
Nonesterified Fatty Acids and Hepatic Glucose Metabolism
Nonesterified Fatty Acids and Hepatic Glucose Metabolism

... uptake) ⫺ (net hepatic lactate output ⫹ net hepatic CO2 production), with all values in glucose equivalents. This calculation omits the contribution of gluconeogenic amino acids other than alanine, but the total of their net hepatic uptakes is no more than that of alanine (⬃1.5 ␮mol 䡠 kg⫺1 䡠 min⫺1 g ...
RNA/DNA catalysts
RNA/DNA catalysts

... groups used for catalysis? structures formed? Know about transesterification & cleavage reactions Know four types of natural catalytic RNAs (group I introns, group II introns, RNase P, small self-cleaving), what reactions they perform, know basics of their secondary and tertiary structure, requireme ...
Alcohols
Alcohols

... -- dissolve in water to some degree -- are more polar than hydrocarbons but less polar than water ...
Role of Krebs Cycle in the Mechanism of Stability Internal Medium
Role of Krebs Cycle in the Mechanism of Stability Internal Medium

... and aerobic processes as well as opposed them anabolic endergonic processes reflecting balance catabolic exergonic processes and anabolic endergonic processes which is the main link of mechanism maintenance stability Internal Energy of an organism and cells of an organism according first law of ther ...
Ion specific effects of sodium and potassium on the catalytic activity
Ion specific effects of sodium and potassium on the catalytic activity

... recent experimental and theoretical studies suggest that monovalent ions do not affect the bulk water structure beyond the first solvent shell and that the Hofmeister series should rather be rationalized in terms of ion–protein or ion-water interactions in the first hydration shell of a protein19. I ...
Full-Text PDF
Full-Text PDF

... generation by conjugation of a drug-like core molecule to a surrounding randomized library peptide via the 10BASEd-T (Figure 1). As the model drug-like core molecule, commercially available 4-iodoacetamidosalicylic acid (Sal-IA) was used because it is one of the smallest pharmaceutical molecules pos ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES

... Metalloproteins represent a large portion of the total proteome. When bound to a protein a metal ion influences both protein stability and function through structural, catalytic or regulatory roles. Discovery of a metal ion cofactor presents new insight into both the structural and functional aspect ...
Model of Skeletal Muscle Energy Metabolism
Model of Skeletal Muscle Energy Metabolism

... MATERIALS S2: METABOLIC REACTIONS FLUX EXPRESSIONS The flux expressions for the compartmentalized lumped metabolic reactions that convert substrates to products in the two subcellular compartments (cytosol and mitochondria) in coupled with the energy controller pairs ATP-ADP and NADH-NAD+ are writte ...
Metabolic reaction network approach for CHO
Metabolic reaction network approach for CHO

... vitamins, amino acids, lipids, nucleic acid precursors, carbohydrates, trace elements, salts, bulk ions and often growth factors and hormones. Components that ensure constant pH levels are also necessary [5]. Historically, the media were designed using a base medium supplemented with several factors ...
DOCX - The Human Proteome Project
DOCX - The Human Proteome Project

... extraordinary detections. Time-of-flight, FT-ICR, and Orbitrap-type instruments are considered as having high mass accuracy (when properly calibrated) in these guidelines. Consider alternate explanations of PSMs that appear to indicate extraordinary results. The spectra should be examined closely to ...
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Biochemistry



Biochemistry, sometimes called biological chemistry, is the study of chemical processes within and relating to living organisms. By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism, biochemical processes give rise to the complexity of life. Over the last decades of the 20th century, biochemistry has become so successful at explaining living processes that now almost all areas of the life sciences from botany to medicine to genetics are engaged in biochemical research. Today, the main focus of pure biochemistry is in understanding how biological molecules give rise to the processes that occur within living cells, which in turn relates greatly to the study and understanding of whole organisms.Biochemistry is closely related to molecular biology, the study of the molecular mechanisms by which genetic information encoded in DNA is able to result in the processes of life. Depending on the exact definition of the terms used, molecular biology can be thought of as a branch of biochemistry, or biochemistry as a tool with which to investigate and study molecular biology.Much of biochemistry deals with the structures, functions and interactions of biological macromolecules, such as proteins, nucleic acids, carbohydrates and lipids, which provide the structure of cells and perform many of the functions associated with life. The chemistry of the cell also depends on the reactions of smaller molecules and ions. These can be inorganic, for example water and metal ions, or organic, for example the amino acids which are used to synthesize proteins. The mechanisms by which cells harness energy from their environment via chemical reactions are known as metabolism. The findings of biochemistry are applied primarily in medicine, nutrition, and agriculture. In medicine, biochemists investigate the causes and cures of disease. In nutrition, they study how to maintain health and study the effects of nutritional deficiencies. In agriculture, biochemists investigate soil and fertilizers, and try to discover ways to improve crop cultivation, crop storage and pest control.
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