A familial inverted duplication/deletion of 2p25.1–25.3
... cut-out of the normal and abnormal chromosomes 2 in G-banding at a resolution of 550 bands. Ideogram of normal and inverted duplicated chromosomes 2p: th 2p25.3 and 2p25.1 bands are depicted in green and red, respectively. The dots represent BAC clones RP11-90H11 (2p25.3, green dot) and RP111B18 (2p ...
... cut-out of the normal and abnormal chromosomes 2 in G-banding at a resolution of 550 bands. Ideogram of normal and inverted duplicated chromosomes 2p: th 2p25.3 and 2p25.1 bands are depicted in green and red, respectively. The dots represent BAC clones RP11-90H11 (2p25.3, green dot) and RP111B18 (2p ...
Nucleosomal structure of sea urchin and starfish sperm chromatin
... (MN+H1) separates into two subfractions. The data from two-dimensional electrophoresis show that both subfractions contain the same set of histones but their DNAs differ in length (Fig. 3a). It is not yet clear whether tae fact that the complete nucleosome displays a double zone demonstrates the het ...
... (MN+H1) separates into two subfractions. The data from two-dimensional electrophoresis show that both subfractions contain the same set of histones but their DNAs differ in length (Fig. 3a). It is not yet clear whether tae fact that the complete nucleosome displays a double zone demonstrates the het ...
Classification of plant-pathogenic mycoplasma
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
Benzimidazole resistance survey for Haemonchus - diss.fu
... molecular tools. The FECRT is labour- as well as cost-intensive and can only provide reliable results once the resistant portion of the population has exceeded at least 25% (Martin et al., 1989). However, advanced statistical analysis methods (Torgerson et al., 2014), in combination with the use of ...
... molecular tools. The FECRT is labour- as well as cost-intensive and can only provide reliable results once the resistant portion of the population has exceeded at least 25% (Martin et al., 1989). However, advanced statistical analysis methods (Torgerson et al., 2014), in combination with the use of ...
Population Genetics I
... The rate of evolution in these latter cases, with 0 ≤ h ≤ 1, is fastest when the rare allele is the dominant one. The rare allele will occur mostly in heterozygotes. If it is dominant, its fitness effect still will be expressed even in the heterozygotes, and selection can act on it (for it, if adva ...
... The rate of evolution in these latter cases, with 0 ≤ h ≤ 1, is fastest when the rare allele is the dominant one. The rare allele will occur mostly in heterozygotes. If it is dominant, its fitness effect still will be expressed even in the heterozygotes, and selection can act on it (for it, if adva ...
Major City Chiefs Position Paper on Sworn vs
... Retention – The Committee recognized that retention rates of sworn personnel are much higher than that of the civilian counterparts. The Committee identified these better retention rates as, often, tied to the police retirement systems that are generally much better than that of the civilian retirem ...
... Retention – The Committee recognized that retention rates of sworn personnel are much higher than that of the civilian counterparts. The Committee identified these better retention rates as, often, tied to the police retirement systems that are generally much better than that of the civilian retirem ...
Molecular Diagnostics in Clinical Microbiology
... forward. Nucleic acid from the potential pathogen is extracted from the clinical sample, subsequently followed by an amplification-detection protocol, preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective s ...
... forward. Nucleic acid from the potential pathogen is extracted from the clinical sample, subsequently followed by an amplification-detection protocol, preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective s ...
Molecular Evolution of Functional Nucleic Acids
... specific to the keratinocyte growth factor (KGF) from two libraries of modified RNA, which contained either 2'-fluoropyrimidine nucleosides or 2'-aminopyrimidine nucleosides, and compared both the aptamers [59]. In this case, the 2'-fluoro modified RNA aptamer showed excellent binding affinity with ...
... specific to the keratinocyte growth factor (KGF) from two libraries of modified RNA, which contained either 2'-fluoropyrimidine nucleosides or 2'-aminopyrimidine nucleosides, and compared both the aptamers [59]. In this case, the 2'-fluoro modified RNA aptamer showed excellent binding affinity with ...
Allele frequencies of AVPR1A and MAOA in the Afrikaner population
... (VNTR) in the promoter region of the MAOA gene, which is located on the X chromosome at Xp11.23.11-15 These VNTRs occur in 2, 3, 3.5, 4 or 5 repeats of 30 base pairs (bp).13,16 These repeats can be classified as either high or low activity alleles, with the 2, 3 and 5 repeats constituting the low ac ...
... (VNTR) in the promoter region of the MAOA gene, which is located on the X chromosome at Xp11.23.11-15 These VNTRs occur in 2, 3, 3.5, 4 or 5 repeats of 30 base pairs (bp).13,16 These repeats can be classified as either high or low activity alleles, with the 2, 3 and 5 repeats constituting the low ac ...
Genetic architecture of intelligence from SNP - cog
... Implications of low MAF: epistasis, additivity and all that Why is most of the variance additive? Where is the epistasis that our wet lab colleagues see every day? If most causal variants are rare (e.g., MAF < 0.1), then when two individuals differ at a locus we likely find AA vs Aa. Very few indiv ...
... Implications of low MAF: epistasis, additivity and all that Why is most of the variance additive? Where is the epistasis that our wet lab colleagues see every day? If most causal variants are rare (e.g., MAF < 0.1), then when two individuals differ at a locus we likely find AA vs Aa. Very few indiv ...
Gel Electrophoresis - Sam Houston State University
... After the run, these bands can be examined only after an appropriate dye or imaging/development technique is used. A tracking dye can be added in the buffer to visualize the mobility front to help decide when to stop the run. If pre-stained standards are used their bands can be seen as the electrop ...
... After the run, these bands can be examined only after an appropriate dye or imaging/development technique is used. A tracking dye can be added in the buffer to visualize the mobility front to help decide when to stop the run. If pre-stained standards are used their bands can be seen as the electrop ...
Molecular markers in Brassica Rapa
... KC907717 and KC907718, respectively. Design and validation of DNA molecular markers Primer sets that could amplify specific regions were designed based on sequence variations found in the BrCRTISO1 genomic sequences of OC and YE cultivars using Primer-Blast tools (http://www.ncbi.nlm.nih.gov/tools/ ...
... KC907717 and KC907718, respectively. Design and validation of DNA molecular markers Primer sets that could amplify specific regions were designed based on sequence variations found in the BrCRTISO1 genomic sequences of OC and YE cultivars using Primer-Blast tools (http://www.ncbi.nlm.nih.gov/tools/ ...
Genome organization of Magnaporthe grisea
... integrated map showed that the linear order of markers along all seven chromosomes in both maps is in good agreement. Thirty of eighty seven markers were derived from cosmid clones that contained the retrotransposon MAGGY (M. grisea gypsy element). Mapping of singlecopy DNA sequences associated with ...
... integrated map showed that the linear order of markers along all seven chromosomes in both maps is in good agreement. Thirty of eighty seven markers were derived from cosmid clones that contained the retrotransposon MAGGY (M. grisea gypsy element). Mapping of singlecopy DNA sequences associated with ...
Structural variations in the human genome
... favorable circumstances. They are therefore present in many individuals. These are recurrent structural variations, meaning that they happen more often. Non-recurrent structural variations on the other hand occur on rare spots in the DNA. Sometimes an individual can even seem to be the only one with ...
... favorable circumstances. They are therefore present in many individuals. These are recurrent structural variations, meaning that they happen more often. Non-recurrent structural variations on the other hand occur on rare spots in the DNA. Sometimes an individual can even seem to be the only one with ...
number of fifty human tumours
... Considering first the main group of 30 tumours on which substantial numbers of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approxi ...
... Considering first the main group of 30 tumours on which substantial numbers of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approxi ...
Laws of Probability: Coin Toss Lab
... In this lab you will make predictions using Punnett Squares, you will then use pennies to simulate the crosses. Then compare the Actual Ratios with the Predicted Ratios. The trait you are studying in this lab is the result of a gene that codes for a short big toe in humans. T represents the dominant ...
... In this lab you will make predictions using Punnett Squares, you will then use pennies to simulate the crosses. Then compare the Actual Ratios with the Predicted Ratios. The trait you are studying in this lab is the result of a gene that codes for a short big toe in humans. T represents the dominant ...
Linkage analysis
... Look for more informative markers that result in higher LOD-scores A series of flanking markers allow for multipoint linkage analysis A series of linked markers gives more confidence (subjective) Flanking markers can also be used to fine-map the linkage interval ...
... Look for more informative markers that result in higher LOD-scores A series of flanking markers allow for multipoint linkage analysis A series of linked markers gives more confidence (subjective) Flanking markers can also be used to fine-map the linkage interval ...
Vitis 37 (3), 119
... The electrophoretically detected degree of genetic similarity between each pair of cultivars studied (Tab. 2) was calculated using the NTSYS-pc package 1.8 developed by ROHFL (Exeter Software, New York, USA). ...
... The electrophoretically detected degree of genetic similarity between each pair of cultivars studied (Tab. 2) was calculated using the NTSYS-pc package 1.8 developed by ROHFL (Exeter Software, New York, USA). ...
Read the Nobel Lecture
... label incorporated into the telomeric end than the end lacking a telomere. I incubated the linear DNA substrate in an extract made from Tetrahymena nuclei. The extract was prepared in a manner that we hoped would allow all of the enzymes normally present in the nuclei to be active. We also added rad ...
... label incorporated into the telomeric end than the end lacking a telomere. I incubated the linear DNA substrate in an extract made from Tetrahymena nuclei. The extract was prepared in a manner that we hoped would allow all of the enzymes normally present in the nuclei to be active. We also added rad ...
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility
... The type I interferon system genes IKBKE and IFIH1 are associated with the risk of systemic lupus erythematosus (SLE). To identify the sequence variants that are able to account for the disease association, we resequenced the genes IKBKE and IFIH1. Eighty-six single-nucleotide variants (SNVs) with p ...
... The type I interferon system genes IKBKE and IFIH1 are associated with the risk of systemic lupus erythematosus (SLE). To identify the sequence variants that are able to account for the disease association, we resequenced the genes IKBKE and IFIH1. Eighty-six single-nucleotide variants (SNVs) with p ...
structure and mechanism of dna polymerases
... The viability of an organism is dependent on the accurate replication of its genome. In general, this is performed with only one error generated for every 109–1010 bases replicated (Echols and Goodman, 1991). This remarkable accuracy is achieved by a combination of different mechanisms working in un ...
... The viability of an organism is dependent on the accurate replication of its genome. In general, this is performed with only one error generated for every 109–1010 bases replicated (Echols and Goodman, 1991). This remarkable accuracy is achieved by a combination of different mechanisms working in un ...
(2) in ppt - NYU Computer Science
... Background Correction & Normalization • Consider a genomic location L and two “similar” nucleotide sequences sL,x and sL,y starting at that location in the two copies of a diploid genomes… – E.g., they may differ in one SNP. – Let qx and qy be their respective copy numbers in the whole genome and al ...
... Background Correction & Normalization • Consider a genomic location L and two “similar” nucleotide sequences sL,x and sL,y starting at that location in the two copies of a diploid genomes… – E.g., they may differ in one SNP. – Let qx and qy be their respective copy numbers in the whole genome and al ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.