Exam 2 question possibility for 2008
... 4. Gramicidin is a 10 amino acid circular oligo-peptide that can integrate into membranes and create a pore through which small ions can freely pass. If yeast cells are treated with gramicidin, which of the following mitochondrial processes should be affected (no explanations required, but may be pr ...
... 4. Gramicidin is a 10 amino acid circular oligo-peptide that can integrate into membranes and create a pore through which small ions can freely pass. If yeast cells are treated with gramicidin, which of the following mitochondrial processes should be affected (no explanations required, but may be pr ...
Manipulating and Analyzing DNA
... In any species genome, there is also a lot of “junk DNA.” This does not perform any known function and, therefore, is free to vary at random. Intraspecific analysis of DNA (sometimes called “DNA fingerprinting”) provides an accurate means of comparison between individuals of the same species. It is ...
... In any species genome, there is also a lot of “junk DNA.” This does not perform any known function and, therefore, is free to vary at random. Intraspecific analysis of DNA (sometimes called “DNA fingerprinting”) provides an accurate means of comparison between individuals of the same species. It is ...
HEA Assay: Red Cell Blood Group Antigen
... The HEA assay is performed on DNA extracted from EDTA whole blood. The DNA region of interest is amplified by multiplex PCR, processed into single-stranded DNA and hybridized with allele-specific oligonucleotide probes to detect the relevant SNPs. The hybridization reaction is performed on a semicon ...
... The HEA assay is performed on DNA extracted from EDTA whole blood. The DNA region of interest is amplified by multiplex PCR, processed into single-stranded DNA and hybridized with allele-specific oligonucleotide probes to detect the relevant SNPs. The hybridization reaction is performed on a semicon ...
Genetics HARDCOPY - New Hartford Central Schools
... 1. Substitution - 1 base is replaced with another 2. Insertion - an extra base is inserted into DNA 3. Deletion - a base is removed from DNA • If a DNA base is changed the protein recipe is changed. • This changes the shape of the protein and it may not function properly • Ex. Sickle cell disease - ...
... 1. Substitution - 1 base is replaced with another 2. Insertion - an extra base is inserted into DNA 3. Deletion - a base is removed from DNA • If a DNA base is changed the protein recipe is changed. • This changes the shape of the protein and it may not function properly • Ex. Sickle cell disease - ...
Biotechnology - BHSBiology-Cox
... by a seiving effect – small molecule navigate the matrix more quickly than larger ones. ...
... by a seiving effect – small molecule navigate the matrix more quickly than larger ones. ...
File
... Hydrogen bonds form between bases chromosomes condense 2. Okazaki fragments form on the: lagging strand leading strand base-pairs 5' end 3. Which of the following is required for DNA replication to occur? DNA helicase DNA ligase DNA polymerase all of these 4. A nucleotide consists of: a nitrogen bas ...
... Hydrogen bonds form between bases chromosomes condense 2. Okazaki fragments form on the: lagging strand leading strand base-pairs 5' end 3. Which of the following is required for DNA replication to occur? DNA helicase DNA ligase DNA polymerase all of these 4. A nucleotide consists of: a nitrogen bas ...
9.3 DNA Fingerprinting
... – The probability that two people share identical numbers of repeats in several locations is ...
... – The probability that two people share identical numbers of repeats in several locations is ...
to view fulltext PDF - Indian Academy of Sciences
... such processes the polymer might undergo a conformational change through stretching and bending. The flexibility of DNA is thus an important parameter defining the molecular interactions. In higher biological systems DNA is in a highly condensed state (called the chromosome) due to its self-assembly ...
... such processes the polymer might undergo a conformational change through stretching and bending. The flexibility of DNA is thus an important parameter defining the molecular interactions. In higher biological systems DNA is in a highly condensed state (called the chromosome) due to its self-assembly ...
word
... RNA) that encodes for the gene of interest – at least 20 nucleotides in length a) Sometimes many probes are needed because the amino acid sequence in question can be encoded by numerous nucleotide sequences (this is called a degenerate “probe”) ...
... RNA) that encodes for the gene of interest – at least 20 nucleotides in length a) Sometimes many probes are needed because the amino acid sequence in question can be encoded by numerous nucleotide sequences (this is called a degenerate “probe”) ...
DNA Restriction and Gel Electrophoresis This laboratory
... multiple times across millions of basepairs, when an entire genome is digested by a restriction enzyme, multiple fragments are usually generated. These fragments will be of various lengths. These pieces of DNA in a gel are usually called ‘bands’. We can compare the whole genomes of two or more indiv ...
... multiple times across millions of basepairs, when an entire genome is digested by a restriction enzyme, multiple fragments are usually generated. These fragments will be of various lengths. These pieces of DNA in a gel are usually called ‘bands’. We can compare the whole genomes of two or more indiv ...
DNA SEQUENCING (using an ABI automated sequencer)
... The other method of sequencing DNA (Sanger 1977) uses a polymerization reaction (using DNA polymerase) in conjunction with a mix of deoxynucleotide triphosphates (dNTPs) and dideoxynucleotide triphosphates (ddNTPs). Since dideoxynucleotides terminate the growth of the DNA polymer once they are incor ...
... The other method of sequencing DNA (Sanger 1977) uses a polymerization reaction (using DNA polymerase) in conjunction with a mix of deoxynucleotide triphosphates (dNTPs) and dideoxynucleotide triphosphates (ddNTPs). Since dideoxynucleotides terminate the growth of the DNA polymer once they are incor ...
GMO Investigator™ Kit - Bio-Rad
... • Extract and amplify DNA from different food samples • Perform genuine diagnostic procedures • Use PCR and electrophoresis to find GMO foods • Sufficient materials for 8 student workstations • Complete the activity in three 45 minute lab sessions ...
... • Extract and amplify DNA from different food samples • Perform genuine diagnostic procedures • Use PCR and electrophoresis to find GMO foods • Sufficient materials for 8 student workstations • Complete the activity in three 45 minute lab sessions ...
PCR of GFP - the BIOTECH Project
... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
Document
... Must provide correct temperature and buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
... Must provide correct temperature and buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
document
... Loci of STR regions found to vary from person to person with a high frequency 13 are used in America, but only 9 are used in Australia – why? ...
... Loci of STR regions found to vary from person to person with a high frequency 13 are used in America, but only 9 are used in Australia – why? ...
Bio 301, Biochemistry I
... amino acid and its appropriate codon in the mRNA-ribosome complex. c. Formation of the ester linkages between a tRNA and its corresponding amino acid is catalyzed by the tRNA itself. d. A tRNA binds to its appropriate amino acid through a covalent linkage of the amino acid’s side chain to the base o ...
... amino acid and its appropriate codon in the mRNA-ribosome complex. c. Formation of the ester linkages between a tRNA and its corresponding amino acid is catalyzed by the tRNA itself. d. A tRNA binds to its appropriate amino acid through a covalent linkage of the amino acid’s side chain to the base o ...
Assembly of microarrays for genome-wide measurement of
... bacteria into 5 ml LB media containing chloramphenicol (10 g/ml) or kanamycin (50 g/ml) for BACs or P1s, respectively and incubated the cultures overnight (~16 h) at 37 C with agitation at 225 rpm. We prepared larger cultures for DNA isolation, by inoculating 25 ml LB media, containing the approp ...
... bacteria into 5 ml LB media containing chloramphenicol (10 g/ml) or kanamycin (50 g/ml) for BACs or P1s, respectively and incubated the cultures overnight (~16 h) at 37 C with agitation at 225 rpm. We prepared larger cultures for DNA isolation, by inoculating 25 ml LB media, containing the approp ...
HIV Vaccine Database and Web Works
... Use chip data first in linkage study, then use same data with transmission-disequilibrium-test for association study within candidate regions. • Have found some relatively common varients that contribute to risk. • Colleagues at UCLA have found rarer, higher risk variants. ...
... Use chip data first in linkage study, then use same data with transmission-disequilibrium-test for association study within candidate regions. • Have found some relatively common varients that contribute to risk. • Colleagues at UCLA have found rarer, higher risk variants. ...
DNA Ladder, Supercoiled (D5292) - Datasheet - Sigma
... Mix an appropriate volume of the Supercoiled DNA Ladder with gel loading buffer (Product No. G2526) to the desired loading concentration. Typically 0.2 µg per well (0.02 µg/µl, 10 µl load) is sufficient to be seen using ethidium bromide staining. The recommended agarose gel concentration is 0.7% (Pr ...
... Mix an appropriate volume of the Supercoiled DNA Ladder with gel loading buffer (Product No. G2526) to the desired loading concentration. Typically 0.2 µg per well (0.02 µg/µl, 10 µl load) is sufficient to be seen using ethidium bromide staining. The recommended agarose gel concentration is 0.7% (Pr ...
Exam 2 Review - Iowa State University
... 30.In what direction is DNA replicated? a) 5’ -> 3’ b) 5’ <- 3” 31.What enzyme removes the first primer and replaces it with DNA? a) DNA polymerase II b) DNA primase c) DNA polymerase I d) DNA ligase 32.The structural feature that allows near-perfect copying of DNA molecules is a) their sugar-phosph ...
... 30.In what direction is DNA replicated? a) 5’ -> 3’ b) 5’ <- 3” 31.What enzyme removes the first primer and replaces it with DNA? a) DNA polymerase II b) DNA primase c) DNA polymerase I d) DNA ligase 32.The structural feature that allows near-perfect copying of DNA molecules is a) their sugar-phosph ...
Genetics Guided Notes: ANSWER KEY Name
... Homozygous Dominant – when an individual has two dominant alleles for a gene Ex : AA Homozygous Recessive – when an individual has two recessive alleles for a gene Ex: aa Heterozygous – when an individual has both a dominant and a recessive allele for a gene ...
... Homozygous Dominant – when an individual has two dominant alleles for a gene Ex : AA Homozygous Recessive – when an individual has two recessive alleles for a gene Ex: aa Heterozygous – when an individual has both a dominant and a recessive allele for a gene ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.