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Teacher Guide - the BIOTECH Project
Teacher Guide - the BIOTECH Project

... Siblings: Between 0% and 100% (baring in mind that all humans are 99.9% the same on a DNA level, these numbers are DNA that you received directly from your parents and that your siblings received directly from your parents.) What are some characteristics or properties of DNA? double-helix, double st ...
Methicillin-resistant Staphylococcus aureus (MRSA) Real
Methicillin-resistant Staphylococcus aureus (MRSA) Real

... and weakened immune systems are at greater risk for infection than the general public. Hospital staff ...
BA13.00
BA13.00

... • Leaves the ends of gene segments “sticky” with usually 3 exposed nucleotides on one side of the double helix, so that ends may be rejoined later. ...
Exam 2
Exam 2

... shown that chi structures generally have two pairs of equal length arms (as shown below). Explain why chi structures exhibit this particular symmetry. Homologous recombination is an exchange of DNA between similar or identical molecules of DNA, such as homologous chromosomes. This symmetry arises be ...
CHP13ABIOH - willisworldbio
CHP13ABIOH - willisworldbio

... • After the foreign DNA has been inserted into the plasmid, the recombined DNA is transferred into a bacterial cell. • An advantage to using bacterial cells to _____ DNA is that they reproduce ______; therefore, millions of bacteria are produced and each bacterium contains hundreds of recombinant D ...
protein synthesis lab
protein synthesis lab

... To understand the three types of point mutations; silent, missense, and nonsense. To understand how an addition or deletion of a nucleotide causes a frameshift mutation. To understand the four types of chromosomal mutations; deletion, duplication, inversion, translocation. ...
CloneEZ PCR Cloning Kit
CloneEZ PCR Cloning Kit

... restriction site in the middle, and the insert-specific sequence at the 3’-end. How to choose the 15-base sequence add-on is illustrated in the graph below. ...
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... C. Replicating this modified DNA millions of times through increase in cell number and the creation of multiple copies of the cloned DNA in each cell. ...
Generuj PDF - Centralne Laboratorium Kryminalistyczne Policji
Generuj PDF - Centralne Laboratorium Kryminalistyczne Policji

... evidential material. The majority of biological traces on examination items is invisible to unaided eye. Laborious work of experts to detect biological stains is supported by optical instrumentation and specialist tests. Human identification of stains is carried out basing on polymorphic characteris ...
Special Study Project III
Special Study Project III

... 28. Restriction enzymes are useful in recombinant DNA studies because they: a. Cut DNA at specific locations b. Join the cut ends of small DNA molecules c. Can reproduce in bacteria d. Give plasmids antibiotic properties e. Can separate pieces of DNA and RNA from each other 29. A readily accessible, ...
answers
answers

... mandrake is dominant to happy looking (r) mandrakes. The mandrakes in his care, which are all offspring of the same cross, are 50% homozygous dominant and 50% heterozygous. What were the genotypes of the parents of these mandrakes? RR and Rr ...
topic 5 : expression of biological information
topic 5 : expression of biological information

... 5 sugar-phosphate bonds form 6 free nucleotides align with complementary nucleotides on each strand In which order do these events take place? A. 1 3 ...
Biol 207 Dr. Locke`s section WS9 Page 1 Workshop 9 Biol207
Biol 207 Dr. Locke`s section WS9 Page 1 Workshop 9 Biol207

... library, genomic DNA from a wild type E. coli will be digested using restriction enzymes into fragements which will then be ligated into a plasmid vector to produce recombinant DNA molecules. The second step involves transforming the recombinant DNA into a mutant (lacZ -) E. coli host strain that ca ...
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Slide 1

... – If chromosome is lost (one copy = monosomic) = individual does not survive – If chromosome is gained (3 copies = trisomic) = individual may survive but only in a few cases and will be mentally impaired • Example: Trisopy 21 (Down syndrome) ...
ch11dna - cpolumbo
ch11dna - cpolumbo

... yielding more than one million copies of the original DNA molecule. Each cycle takes less than two minutes from start to finish. Chapter 11 ...
Gene Cloning
Gene Cloning

... • There is a reluctance on the part of some cultures and individuals to accept the concept of transgenesis, without which gene cloning could not be accomplished • Some cloned genes are used in ‘engineering’ food crops, and food safety has become an issue with the public • There has been a move to pa ...
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F. Mutation and Repair 1. Background on DNA Mutations

... a. Mutation rates are extremely low but are an essential component of evolutionary change • Mutations that become part of the multicellular genome must occur in the cells of the germ line • Somatic mutations may or may not affect the individual but cannot affect the population • Low rates of mutatio ...
Chapter 5
Chapter 5

... advancing complexity of living organisms. 1. The idea is that during meiosis in sexually reproducing organisms, crossover mutations can form multiple copies of a gene, a chromosome or the entire genome. 2. The organism survived just fine with one copy so it only repairs damages (mutations) to one co ...
Lecture_note_463BI
Lecture_note_463BI

... -The dbSNP accepts apparently neutral polymorphisms, polymorphisms corresponding to known phenotypes, and regions of no variation. -It was created in September 1998 to supplement GenBank (NCBI’s nucleic acid and protein sequences) Goal Its goal is to act as a single database that contains all identi ...
03-131 Genes, Drugs, and Diseases Exam 2 – F2015 Name:____________________
03-131 Genes, Drugs, and Diseases Exam 2 – F2015 Name:____________________

... Temperature is raised to the optimal temperature for polymerase, polymerase synthesizes a copy of the template by adding bases to the primer. Choice B: ddNTP is a di-deoxy nucleotide tri phosphate. It has three phosphates on its 5’ carbon so it can be incorporated into the growing DNA, but since it ...
Unit review questions
Unit review questions

... 52. What is RNA polymerase & tell its function. 53. What are promoters? 54. Where does RNA polymerase bind to the DNA it is transcribing? 55.What makes the beginning of a new gene on DNA in eukaryotes? 56. What do promoters mark the beginning of on prokaryotic DNA? 57. When a promoter binds to DNA, ...
SBI-4U1 Exam Review
SBI-4U1 Exam Review

... DNA is negatively charged. It will migrate away from the negative electrode, towards the positive. ...
DNA upgrade supplement WITH PICS
DNA upgrade supplement WITH PICS

... Griffith isolated the transformed bacteria from dead mice and grew them in the lab, they continued to produce smooth colonies. Griffith discovered that genetic material can be transferred, but he did not know which substance acted as genetic material. A series of related experiments soon provided th ...
Lecture 26: Overview of deoxyribonucleic acid (DNA) and
Lecture 26: Overview of deoxyribonucleic acid (DNA) and

... DNA can exist in 3 forms A, B & Z. Although only B- DNA and Z-DNA have been directly observed in functional organism. The conformation that DNA adopts depends on the hydration level, DNA sequence, the amount and direction of supercoiling, chemical modification of bases, type and concentration of met ...
4.4 PCR, Electrophoresis, DNA profiling
4.4 PCR, Electrophoresis, DNA profiling

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SNP genotyping



SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.
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