PCR of GFP - the BIOTECH Project
... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
supplementary materials
... Under the experimental conditions, P [DNA]; thus we can approximate the free protein concentration by the total protein concentration, and use the following equation to ...
... Under the experimental conditions, P [DNA]; thus we can approximate the free protein concentration by the total protein concentration, and use the following equation to ...
Conceptual Questions C1. Answer: A. G→A, which is a transition. B
... affected with a TNRE disorder, he or she might be concerned that an expansion of the repeat might occur during gamete formation, yielding offspring more severely affected with the disorder, a phenomenon called anticipation. This phenomenon may depend on the sex of the parent with the TNRE. C19. Answ ...
... affected with a TNRE disorder, he or she might be concerned that an expansion of the repeat might occur during gamete formation, yielding offspring more severely affected with the disorder, a phenomenon called anticipation. This phenomenon may depend on the sex of the parent with the TNRE. C19. Answ ...
Session 2 – Origin of Life
... “Life does not arise spontaneously but comes only from prior life, through reproduction… Although life certainly originated from ...
... “Life does not arise spontaneously but comes only from prior life, through reproduction… Although life certainly originated from ...
RECOMBINANT DNA TECHNOLOGY: APPLICATIONS IN THE
... fragment length polymorphisms (RFLPs), short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). All three can occur within genes as well as in intergenic regions, and altogether there are several million of these polymeric sites I the human genome, with SNPs being the most common. Gen ...
... fragment length polymorphisms (RFLPs), short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). All three can occur within genes as well as in intergenic regions, and altogether there are several million of these polymeric sites I the human genome, with SNPs being the most common. Gen ...
homologous structures
... C. Single-celled organisms D. Can be killed with antibiotics E. None of the above ...
... C. Single-celled organisms D. Can be killed with antibiotics E. None of the above ...
CHAPTER 18
... was mildly affected with a TNRE disorder, he or she might be concerned that an expansion of the repeat might occur during gamete formation, yielding offspring more severely affected with the disorder, a phenomenon called anticipation. This phenomenon may depend on the sex of the parent with the TNRE ...
... was mildly affected with a TNRE disorder, he or she might be concerned that an expansion of the repeat might occur during gamete formation, yielding offspring more severely affected with the disorder, a phenomenon called anticipation. This phenomenon may depend on the sex of the parent with the TNRE ...
DNA sequence representation by trianders and determinative
... separate walks characterized by different angles and lengths, and that such an object is called triander which reflects the “strength” of branch. A general method for identifying DNA sequence “by triander” which can be treated as a unique “genogram” (or “gene passport”) is proposed. The two- and thr ...
... separate walks characterized by different angles and lengths, and that such an object is called triander which reflects the “strength” of branch. A general method for identifying DNA sequence “by triander” which can be treated as a unique “genogram” (or “gene passport”) is proposed. The two- and thr ...
protein synthesis worksheet
... PART A. Read the following and answer Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcribes (copies) DNA. DNA is “unzipped” and the mRNA strand copies a strand ...
... PART A. Read the following and answer Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcribes (copies) DNA. DNA is “unzipped” and the mRNA strand copies a strand ...
PROTEIN-SYNTHESIS
... PROTEIN SYNTHESIS WORKSHEET PART A. Read the following and take notes on your paper: Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcribes (copies) DNA. DNA is ...
... PROTEIN SYNTHESIS WORKSHEET PART A. Read the following and take notes on your paper: Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcribes (copies) DNA. DNA is ...
protein synthesis worksheet
... Name____________________ Period____________ PROTEIN SYNTHESIS WORKSHEET PART A. Read the following and answer Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcri ...
... Name____________________ Period____________ PROTEIN SYNTHESIS WORKSHEET PART A. Read the following and answer Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcri ...
Fluorescent Protein - The Fluorescence Foundation
... the 5'-3' polymerase activity adds nucleotides to all the available 3' ends created by the DNase . This exonuclease/polymerase activity, moves (or "translates") any single stranded nick in the 5'-3' direction. When nicks on opposite strands meet, the DNA molecule breaks ...
... the 5'-3' polymerase activity adds nucleotides to all the available 3' ends created by the DNase . This exonuclease/polymerase activity, moves (or "translates") any single stranded nick in the 5'-3' direction. When nicks on opposite strands meet, the DNA molecule breaks ...
Page | 244 - FTHS Wiki
... Purpose: To determine why cells are small and what role diffusion has in cell size. Problem: ____________________________________________________________________________________________________________ __________________________________________________________________________________________________ ...
... Purpose: To determine why cells are small and what role diffusion has in cell size. Problem: ____________________________________________________________________________________________________________ __________________________________________________________________________________________________ ...
Decoding the Language of Genetics
... DNA, chromosomes, proteins, or even “information” in the modern sense (i.e., something that can be reduced to and faithfully transmitted as a string of binary digits). Nevertheless, it is possible to restate his central insight in modern language. Mendel realized that the experimentally reproducible ...
... DNA, chromosomes, proteins, or even “information” in the modern sense (i.e., something that can be reduced to and faithfully transmitted as a string of binary digits). Nevertheless, it is possible to restate his central insight in modern language. Mendel realized that the experimentally reproducible ...
Chromosomes, DNA, and Genes
... 1. DNA is a recipe for _____________. 2. What is a gene? 3. How many genes does a chromosome hold? 4. Where are chromosomes stored in the cell? 5. How many chromosomes do humans have? 6. What organism has the most chromosomes? 7. What organism has the least chromosomes? 8. How many sex chromosomes d ...
... 1. DNA is a recipe for _____________. 2. What is a gene? 3. How many genes does a chromosome hold? 4. Where are chromosomes stored in the cell? 5. How many chromosomes do humans have? 6. What organism has the most chromosomes? 7. What organism has the least chromosomes? 8. How many sex chromosomes d ...
DNA repair disorders
... DNA is continually subjected to both exogenous and endogenous mutagenesis. Cells have built up sophisticated mechanisms to minimise the eVects of this. Mutations in actively transcribed genes are preferentially repaired and all DNA should be repaired before DNA replication when a mutation can become ...
... DNA is continually subjected to both exogenous and endogenous mutagenesis. Cells have built up sophisticated mechanisms to minimise the eVects of this. Mutations in actively transcribed genes are preferentially repaired and all DNA should be repaired before DNA replication when a mutation can become ...
Chromosomes, DNA, and Genes
... Chromosomes • Contained within the nucleus of a cell • One long thread of DNA that is tightly coiled • DNA Chromosome Wrapping ...
... Chromosomes • Contained within the nucleus of a cell • One long thread of DNA that is tightly coiled • DNA Chromosome Wrapping ...
Gene Therapy
... Therefore, the design of hydrogel materials that allow for cellular infiltration and deliver genes to infiltrating cells would be ideal for regeneration of tissues in vivo. Cellular infiltration is migration of cells from their sources of origin, or direct extension of cells as a result of unusual ...
... Therefore, the design of hydrogel materials that allow for cellular infiltration and deliver genes to infiltrating cells would be ideal for regeneration of tissues in vivo. Cellular infiltration is migration of cells from their sources of origin, or direct extension of cells as a result of unusual ...
HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE
... HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE REAL TIME PCR HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE REAL TIME PCR (Real Time PCR – Taqman technology) ...
... HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE REAL TIME PCR HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE REAL TIME PCR (Real Time PCR – Taqman technology) ...
Chapter 10
... Cells then use 2 different types of RNA to read the instructions on the RNA molecule and put together the amino acids that make up the protein in a process called translation. ...
... Cells then use 2 different types of RNA to read the instructions on the RNA molecule and put together the amino acids that make up the protein in a process called translation. ...
Textbook Reference: Section 17.3
... discovered that during replication, much of the newly-formed DNA could be found in short fragments of a few hundred nucleotides (in eukaryotes). They became known as Okazaki fragments, and they occur during the elongation of the daughter DNA strand that must be built in the 3' to 5' direction. As il ...
... discovered that during replication, much of the newly-formed DNA could be found in short fragments of a few hundred nucleotides (in eukaryotes). They became known as Okazaki fragments, and they occur during the elongation of the daughter DNA strand that must be built in the 3' to 5' direction. As il ...
A-level Biology B Question paper Unit 2 - Genes and Genetic
... (b) Spindle fibres are polymers made from tubulin monomers. The removal of tubulin monomers causes spindle fibres to shorten. Scientists investigated the effect of the rate of tubulin removal on the speed of movement of chromatids during mitosis. The results are shown on the graph. ...
... (b) Spindle fibres are polymers made from tubulin monomers. The removal of tubulin monomers causes spindle fibres to shorten. Scientists investigated the effect of the rate of tubulin removal on the speed of movement of chromatids during mitosis. The results are shown on the graph. ...
Laboratory 9: Plasmid Isolation
... 1. The day before lab, pick 4 transformants from each ligation with separate sterile toothpicks, and inoculate separate culture tubes containing liquid medium (LB+Kan). 2. Grow overnight with shaking at 37ºC. Day of lab: B. Harvest and lyse bacteria. 1. Transfer 1.4 mL of bacterial cultures to a ste ...
... 1. The day before lab, pick 4 transformants from each ligation with separate sterile toothpicks, and inoculate separate culture tubes containing liquid medium (LB+Kan). 2. Grow overnight with shaking at 37ºC. Day of lab: B. Harvest and lyse bacteria. 1. Transfer 1.4 mL of bacterial cultures to a ste ...
Nucleic acid double helix
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.