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Chapter 18 - Canyon ISD
Chapter 18 - Canyon ISD

... • Phages carry bacterial genes from one host cell to another – Generalized transduction: some of the host’s DNA gets packaged with the new phage during lytic cycle and transferred to a new host – Specialized transduction: requires infection from a temperate phage, and a piece of the prophage gets pi ...
Unit 4
Unit 4

... nucleotides of each strand and what type of bond holds the two strands together.  DNA is a double helix. Purine and pyrimidine bases are stacked. There are ten layers of nitrogenous base pairs in each turn of the helix. Hydrogen bonds hold the bases together. Explain, in their own words, semiconser ...
Genetics - true or false
Genetics - true or false

... Most of your DNA is found in the cell nucleus. Mitochondria (types of cell organelle) also have a small amount of their own DNA. All human cells contain DNA (except for mature red blood cells). If students consider the statement is false, they are technically correct but be aware of the common misun ...
DNA Structure and Function
DNA Structure and Function

... • Every cell in your body came from 1 original egg and sperm • Every cell has the same DNA and the same genes • Each cell is different, specialized • Differences due to gene expression – Which genes are turned on – When the genes are turned on – How much product they make ...
Chapter 24
Chapter 24

... • Pol I is composed of two domains, N-ter “small fragment” which has 5’→3’ exonuclease function and C-ter “large fragment” which has polymerase and 3’→5’ exonuclease function. • The large fragment is called Klenow fragment. It looks like a right hand shape. • The major roles of Pol I are to repair t ...
Producing the Bovine Growth Hormone
Producing the Bovine Growth Hormone

... chromosome and can replicate itself. In order to insert the BGH gene into the plasmid, the plasmid must be removed from the bacterial cell and cut open with the same restriction enzyme used on the cow DNA. ...
Sample Examination Questions for Exam 2 Material Warning!
Sample Examination Questions for Exam 2 Material Warning!

... Briefly explain the two major lines of evidence used by Watson and Crick to deduce the structure of the B-DNA helix. (6 pt) X-ray diffraction crystallography of DNA fibers showed that DNA was in the form of a helix, and from the diffraction pattern Watson and Crick were able to calclulate the dimens ...
5.2.3 Genomes and Gene Technologies
5.2.3 Genomes and Gene Technologies

... DNA sample mixed with DNA nucleotides and DNA polymerase enzyme Heated to 95⁰C breaking hydrogen bonds to make sample single stranded Short lengths of single stranded DNA added (called primers) Temperature reduced to 55⁰C allowing primers to bind (H bonds) and form small double stranded DNA sections ...
Bacterial Genetics
Bacterial Genetics

... a group of genes associated with other segments of DNA regulated as a whole by regulatory gene (not part of operon)  The regulatory gene controls a part of the operon know as the operator gene = single “on/off” switch for the SG’s collectively Operons = operator + promoter + (few to many) SG  The ...
Viruses and Bacteria
Viruses and Bacteria

... Transduction -In the process known as transduction, phages carry bacterial genes from one host cell to another Conjugation -Conjugation is the direct transfer of genetic material between bacterial cells that are temporarily joined (Pili) ...
Fig1 from Nature Rev Mol. Cell Biol (Nov2003) 4(11):865
Fig1 from Nature Rev Mol. Cell Biol (Nov2003) 4(11):865

... ‘mobile’ DNA: transposable elements ...
Transcription and Translation Candy Activity
Transcription and Translation Candy Activity

... of the 4 RNA nucleotides clearly labeling: ribose, base, phosphate group and nucleotide name. ...
Cloning and Sequencing
Cloning and Sequencing

... Length of primer is generally 18-30 nucleotides G/C content and intra-complementarity are a concern when designing primers Actually not a single primer for each but a mixture of primers (oligoprimers) if the sequence of the target is not known If amino acid sequence of gene product is used then dege ...
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Unit 4
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... A codon is a mRNA base triplet. The relationship that exists between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide are Explain the process of transcription including the three major steps of initiation, elongation, and termination. As an RNA polymeras ...
point mutation
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... from 5’ to 3’. As it does this, the DNA continues to separate and then there is a gap where polymerization has to start again. This creates [Okazaki] fragments instead of one continuous strand. ...
Exam3-1406_Spring'06.doc
Exam3-1406_Spring'06.doc

... D) A = U and G = C in any molecule of RNA. E) DNA and RNA are made up of the same four nitrogenous bases. 10) The X-ray diffraction pattern for DNA suggested to Wilkins and Franklin all of the following features about DNA EXCEPT A) a DNA molecule is helical. B) a DNA molecule has a diameter of 2 nan ...
Inheritence Lecture
Inheritence Lecture

... By program I mean an organized plan that describes both the ingredients themselves and the kinetics of the interactions among ingredients as the living system persists through time. For the living systems we observe on Earth, this program is implemented by the DNA that encodes the genes of Earth's o ...
1 Biotechnology: Old and New
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... particles. d) Analysis showed that the labeled DNA was inside of the bacteria. ...
Final Exam 4a - Buffalo State College Faculty and Staff Web Server
Final Exam 4a - Buffalo State College Faculty and Staff Web Server

... A. If they can both asexually reproduce and their offspring can also successfully asexually reproduce, then they are all the same species. B. If they mate successfully and their offspring can also mate successfully, all are the same species. C. If they mate when they are put together then they are t ...
Lecture 18
Lecture 18

... iv. All of DNA is double stranded v. RNA can be double or single stranded vi. Evidence for model that RNA precedes DNA 1. RNA involved in synthesis of both itself and DNA 2. DNA cannot synthesize itself, it only provides the encoding 3. Diagram of templates 4. RNA ubiquitous in all DNA functions 5. ...
Biotechnology and Recombinant DNA
Biotechnology and Recombinant DNA

... interest from its genomic source and putting it in an expression vector. Steps: 1. Obtain the gene (PCR, restriction digest) 2. Ligate it into a vector (vector = carrier piece of DNA) 3. Transform the new recombinant DNA into bacteria/cells 4. Grow up a population of transformed cells that contain t ...
2140401 - Gujarat Technological University
2140401 - Gujarat Technological University

... List of Open Source Software/learning website: Students can refer to video lectures available on the websites including NPTEL. Students can refer to the CDs which are available with some reference books. Students can develop their own flowsheets for demonstration of central dogma process. ACTIVE LEA ...
Ch. 16 - Harford Community College
Ch. 16 - Harford Community College

... • Most are single-stranded RNA viruses. They enter plant cells through damaged cell walls or are inherited from a parent. ...
JRA1 - Del. 4.3
JRA1 - Del. 4.3

... However working with leading researchers in the field of ancient DNA results appear to be very promising. The following from a recently submission to a high profile publication illustrates the steps within thermal-age.eu and the quality of the prediction (references not given). A thermal age (Smith ...
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Nucleic acid double helix



In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.
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