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幻灯片 1 - TUST
幻灯片 1 - TUST

... Frequently Gene-specific probes are constructed with cDNA clones. If the gene of interest is expressed in a specific tissue or cell type, its mRNA is often relatively abundant. Although mRNA is not available in sufficient quantity to serve as a probe, the desired mRNA species can be converted into c ...
Prenatal Testing for Genetic Disorders
Prenatal Testing for Genetic Disorders

...  Testing for a wide range of genetic disorders is possible using DNA chips (microarrays), which can hold thousands of genes  http://learn.genetics.utah.edu/content/labs/micro ...
Biotechnology
Biotechnology

... – Differences in “polymorphic regions” between the genes on the DNA. ...
Mutations (power point)
Mutations (power point)

... another nucleotide pair is called a base-pair substitution. • Some base-pair substitutions have little or no impact on protein function. – In silent mutations, alterations of nucleotides still indicate the same amino acids because of redundancy in the genetic code. – Other changes lead to switches f ...
AP Test Genetics Review
AP Test Genetics Review

... growth, protein synthesis, and chromosome duplication occurs. Has 3 sub-phases: • G1 phase: “first gap” the cell grows • S phase: “synthesis” chromosomes duplicate • G2 phase: “second gap” the cell grows some more and prepares for division ...
My Genetic Profile Worksheet
My Genetic Profile Worksheet

... • The mRNA delivers this genetic instruction into the cytoplasm where it is used to make a protein or part of a protein (a polypeptide) • RNA is very similar to DNA in that for every base in the DNA gene sequence, the cell assembles a matching RNA base. • Therefore the mRNA molecule will have a set ...
Unit 4
Unit 4

... Basing their model on data from Franklin’s X-ray diffraction photo of DNA, Watson and Crick discovered that DNA is a double helix. Two anti-parallel sugar-phosphate chains wind around the outside of the molecule; the nitrogenous bases project into the interior, where they hydrogenbond in specific pa ...
Gene Regulation III Reminder
Gene Regulation III Reminder

... • genetically active ...
Preparation of SCRATCHY Hybrid Protein Libraries
Preparation of SCRATCHY Hybrid Protein Libraries

... SCRATCHY is a combination of the incremental truncation for the creation of hybrid enzymes (ITCHY) technology (1) and DNA shuffling (2). It generates combinatorial libraries of hybrid proteins consisting of multiple fragments from two or more parental DNA sequences with no restriction to DNA sequenc ...
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Patent Law Prof. Merges

... • Chromosomes: 80 million – 110 million base pairs • Genes: 80,000 bp • Coding region: 10,200 bp ...
bio 15 midterm exam 2 qa 141112
bio 15 midterm exam 2 qa 141112

... d. NaCl e. H2O 2. The following molecules are considered polymers except……Mark all that apply a. Starch b. DNA c. Proteins d. Lipids e. Salt 3. Which is the correct term for compounds that do mix with water? a. phospholipids b. hydrophobic c. hydrophilic d. protein e. hydrogen bonded 4. Which of the ...
Invertebrate epigenomics: the brave new world of
Invertebrate epigenomics: the brave new world of

... the universal nature of such regulatory organization. Nowadays, it is believed that the massive phenotypic disparities observed between organisms with similar gene numbers are generated through regulatory differences. A more nuanced understanding of species-specific, epigenomic adaptations will be n ...
Lecture 6 S - BEHESHTI MAAL
Lecture 6 S - BEHESHTI MAAL

... Frame shift mutation – One or a few nucleotide pairs are deleted or inserted in the DNA – Shifts the translation reading frame – Almost always result in a long stretch of altered amino acids – Inactive protein ...
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Question How does DNA control a cell?By controlling Protein

... between the new AA and the polypeptide chain in the P-site. ...
Chapter 11: DNA: The Molecule of Heredity
Chapter 11: DNA: The Molecule of Heredity

... shape of DNA is a double helix, which is like a twisted ladder. The sides of the ladder are made of alternating sugar and phosphate molecules. The sugar is a pentose called deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the deoxyribose sugars blue (one is labeled w ...
Day 4. Genes and Genetic Level of Organization
Day 4. Genes and Genetic Level of Organization

... DNA—genetic material made out of protein that tells us what traits we inherit. Chromosome—bundles of DNA (genetic instructions) that are stored and X or Y shaped. Chromosomes specify what type of traits offspring will have.. Nucleus- Contains chromosomes and are found in eukaryotic cells. Chromosome ...
Chapter 2 Human Genetics Overview The purpose of this chapter is
Chapter 2 Human Genetics Overview The purpose of this chapter is

... The results are also useful for biological anthropologists in comparisons between species. In addition to the discovery that “junk DNA” is not all junk there has been work in epigenetics. o Which genes are expressed in different cells (cell differentiation) is a focus of the study of epigenetics (th ...
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Slide 1

... b. RNA splicing is considered an important rather than a wasteful process? Explain. RNA splicing makes genetic recombination between exons of different genes more likely, leading to formation of different mRNAs and evolution of genes for new proteins. ...
Genetics 314 - Spring 2005
Genetics 314 - Spring 2005

... heating and cooling the DNA. a) What information would heating the DNA tell you and would this help in identifying the source organism for each sample? By heating the DNA you could tell what temperature it would denature which is related to the amount of G – C pairs and A – T pairs in the DNA sample ...
AWC Summer Studentship Report_Will Stovall
AWC Summer Studentship Report_Will Stovall

... that GBS is highly reproducible, and can reach previously inaccessible regions of the genome. They also assert that the approach is exceptionally useful for conservation studies, as it can help infer population structure in the absence of a reference genome or prior knowledge of diversity in the spe ...
DNA Fingerprinting
DNA Fingerprinting

... The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNKA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a tumor suppressor genes such as one called p53. These genes usually keep cells from div ...
Introduction to molecular biology
Introduction to molecular biology

... In the early ‘60s, F. Jacob and J. Monod − two French biochemists − were the first to obtain experimental evidence on how cells distinguish between genes that should or should not be transcribed Their work on the regulation of prokaryotic genes (Nobel 1965) revealed that the expression of the struct ...
Chapter 12
Chapter 12

... 12.4 Reverse transcriptase can help make genes for cloning A.) Complementary DNA (cDNA) is used to clone eukaryotic genes – mRNA from a specific cell type is the template – Reverse transcriptase produces a DNA strand from mRNA – DNA polymerase produces the second DNA strand ...
Allele: alternative form of a gene, e
Allele: alternative form of a gene, e

... Messenger RNA (mRNA): DNA of a gene is transcribed into mRNA molecules, which then serve as a template for the synthesis of proteins. Metabonome: constituent metabolites in a biological sample. Metabonomics: techniques available to identify the presence and concentrations of metabolites in a biologi ...
Diapositive 1
Diapositive 1

... NH3 (Alanine) ...
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Nucleic acid double helix



In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.
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