video slide - Wesleyan College Faculty
... Colonies of cells containing the gene of interest have been identified by nucleic acid hybridization. Cells from colonies tagged with the probe can be grown in large tanks of liquid growth medium. Large amounts of the DNA containing the gene of interest can be isolated from these cultures. By using ...
... Colonies of cells containing the gene of interest have been identified by nucleic acid hybridization. Cells from colonies tagged with the probe can be grown in large tanks of liquid growth medium. Large amounts of the DNA containing the gene of interest can be isolated from these cultures. By using ...
DNA / RNA
... • Transcription: transfer of information from DNA to RNA in the nucleus In the process of transcription DNA is used to make a complimentary strand of RNA o The WHOLE strand of DNA doesn’t get turned into RNA at once o Instead, as needed, small sections of DNA are transcribed into RNA • Sections are ...
... • Transcription: transfer of information from DNA to RNA in the nucleus In the process of transcription DNA is used to make a complimentary strand of RNA o The WHOLE strand of DNA doesn’t get turned into RNA at once o Instead, as needed, small sections of DNA are transcribed into RNA • Sections are ...
Histone genes of Volvox carteri: DNA sequence and organization of
... Nucleic Acids Research tion. By comparing the sequences of two such H3-H4 l o c i we intended to identify conserved signal structures essential for transcriptional control, elucidate the overall gene organization and detect features relevant to their evolution. ...
... Nucleic Acids Research tion. By comparing the sequences of two such H3-H4 l o c i we intended to identify conserved signal structures essential for transcriptional control, elucidate the overall gene organization and detect features relevant to their evolution. ...
Pan-cancer analysis of the metabolic reaction network
... fraction of the models. Absent reactions are absent in virtually all models. (B) Number of core, contextual and absent reactions in the pan-cancer set and in a random set of 917 metabolic networks. The error bar represents the 95% confidence interval for the bootstrap statistics. See also Fig. S5. ( ...
... fraction of the models. Absent reactions are absent in virtually all models. (B) Number of core, contextual and absent reactions in the pan-cancer set and in a random set of 917 metabolic networks. The error bar represents the 95% confidence interval for the bootstrap statistics. See also Fig. S5. ( ...
Correlating mRNA and protein Abundance
... 441) have significantly uncorrelated mRNA and protein levels (2STDEV from trendline) Transcription Assoc. genes are 25% of the essential genes in yeast. Essential Genes as a group have higher correlations than the general yeast population 7% of Cell Cycle associated genes (n = 432) have ...
... 441) have significantly uncorrelated mRNA and protein levels (2STDEV from trendline) Transcription Assoc. genes are 25% of the essential genes in yeast. Essential Genes as a group have higher correlations than the general yeast population 7% of Cell Cycle associated genes (n = 432) have ...
Open Reading Frame (ORF) finding - Manatee
... patterns of the genome as a whole. Often when viewing a 6-frame translation, the genes are represented as arrows drawn above (or, as in this slide, below) the 6-frame translation. ...
... patterns of the genome as a whole. Often when viewing a 6-frame translation, the genes are represented as arrows drawn above (or, as in this slide, below) the 6-frame translation. ...
The codon adaptation index-a measure of directional synonymous
... their expression, and for detecting sequencing errors. However, the statistic is not normalized and therefore the values for two genes encoding proteins with different araino acid compositions can be quite different even if both genes use only the "best" codons. With various purposes in mind we have ...
... their expression, and for detecting sequencing errors. However, the statistic is not normalized and therefore the values for two genes encoding proteins with different araino acid compositions can be quite different even if both genes use only the "best" codons. With various purposes in mind we have ...
Inquiry into Life Twelfth Edition
... • 5’-end of the first gRNA hybridizes to an unedited region at the 3’-border of editing I the pre-mRNA • The 5’-ends of the rest of the gRNAs hybridize to edited regions progressively closer to the 5’-end of the region to be edited in the pre-mRNA • All of these gRNAs provide A’s and G’s as template ...
... • 5’-end of the first gRNA hybridizes to an unedited region at the 3’-border of editing I the pre-mRNA • The 5’-ends of the rest of the gRNAs hybridize to edited regions progressively closer to the 5’-end of the region to be edited in the pre-mRNA • All of these gRNAs provide A’s and G’s as template ...
GPR17 shRNA Plasmid (r): sc-270023-SH
... GPR17 shRNA Plasmid (r) is a target-specific lentiviral vector plasmid encoding a 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 20 µg of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also see GPR17 siRNA (r): sc-270023 and GPR17 shRNA ...
... GPR17 shRNA Plasmid (r) is a target-specific lentiviral vector plasmid encoding a 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 20 µg of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also see GPR17 siRNA (r): sc-270023 and GPR17 shRNA ...
View Full PDF - Biochemical Society Transactions
... of how the two TORCs operate in this regard. The initial observation that TOR regulates growth was made in yeast with the demonstration that rapamycinsensitive TORC1 promotes protein synthesis when nutrient conditions are favourable for yeast growth [29]. However, the ability of TORC1 to couple nutr ...
... of how the two TORCs operate in this regard. The initial observation that TOR regulates growth was made in yeast with the demonstration that rapamycinsensitive TORC1 promotes protein synthesis when nutrient conditions are favourable for yeast growth [29]. However, the ability of TORC1 to couple nutr ...
T-DNA Mutagenesis
... is when a mutation is created in such a way that death does not occur so as to observe the effects on the plant by the loss of a certain gene. In other words, a gene is knocked out and the plant is grown and observed for any differences between the mutant strain and the control strain. Thus facilita ...
... is when a mutation is created in such a way that death does not occur so as to observe the effects on the plant by the loss of a certain gene. In other words, a gene is knocked out and the plant is grown and observed for any differences between the mutant strain and the control strain. Thus facilita ...
Unique Protein Reporter Assays: Green Fluorescent Proteins (GFP
... This innovative GFP system increases S/N signal, solves solubility/aggregation issues, and, next, protein/protein interactions. EvoGlow GFP This unique fluorescent reporter protein can be used in both aerobic and anaerobic biological systems Single and Dual Luciferase assays, using Purple Cypridina, ...
... This innovative GFP system increases S/N signal, solves solubility/aggregation issues, and, next, protein/protein interactions. EvoGlow GFP This unique fluorescent reporter protein can be used in both aerobic and anaerobic biological systems Single and Dual Luciferase assays, using Purple Cypridina, ...
molecular biology
... information necessary for proper functioning of the cell as well as transfers characters from one generation to other, efforts were made to understand its structure, replication and the pathway for deciphering the coded information to physiologically functional form. The discovery of DNA structure b ...
... information necessary for proper functioning of the cell as well as transfers characters from one generation to other, efforts were made to understand its structure, replication and the pathway for deciphering the coded information to physiologically functional form. The discovery of DNA structure b ...
b-Globin locus control region HS2 and HS3 interact structurally and
... the erythroid transcription factor EKLF (25,26), and work from several laboratories has implicated EKLF in HS3 function and hypersensitivity (27±30). K562 cells, which have an embryonic/fetal phenotype, express neither EKLF nor the adult b-globin mRNA (31), and HS3 sequences in the natural K562 b-gl ...
... the erythroid transcription factor EKLF (25,26), and work from several laboratories has implicated EKLF in HS3 function and hypersensitivity (27±30). K562 cells, which have an embryonic/fetal phenotype, express neither EKLF nor the adult b-globin mRNA (31), and HS3 sequences in the natural K562 b-gl ...
The nucleotide sequence of the gene encoding the attachment
... proposed, phocine distemper virus (PDV), responsible for distemper in seals. It is now well established that morbillivirus virions contain six proteins (Rima, 1983): the nucleocapsid (N) protei n, the phosphoprotein (P), the large (L) protein, the matrix protein (M) and two integral membrane protein ...
... proposed, phocine distemper virus (PDV), responsible for distemper in seals. It is now well established that morbillivirus virions contain six proteins (Rima, 1983): the nucleocapsid (N) protei n, the phosphoprotein (P), the large (L) protein, the matrix protein (M) and two integral membrane protein ...
cDNA, genomic sequence cloning and overexpression of ribosomal
... al., 1996). In addition, L9 has been implicated as a participant in a recoding event known as “ribosomal hopping”, where L9 mutations have been associated with the ability of ribosomes to bypass a 50-nucleotide region within the coding region of the bacteriophage T4 gene 60 mRNA (Herbst et al., 1994 ...
... al., 1996). In addition, L9 has been implicated as a participant in a recoding event known as “ribosomal hopping”, where L9 mutations have been associated with the ability of ribosomes to bypass a 50-nucleotide region within the coding region of the bacteriophage T4 gene 60 mRNA (Herbst et al., 1994 ...
Reprinted from New Beer
... theories on the origins of the first living beings. One of the first to incorporate enzymes into the discussions of the origin of life was Leonard T. Troland (1914, 1917), who proposed that the first living system had been a primordial self-replicating enzyme whose catalytic activity influenced its ...
... theories on the origins of the first living beings. One of the first to incorporate enzymes into the discussions of the origin of life was Leonard T. Troland (1914, 1917), who proposed that the first living system had been a primordial self-replicating enzyme whose catalytic activity influenced its ...
An Introduction to the Cytoskeleton.
... microinjection of stable actin nuclei. Recently, it has come to light that certain are capable of using the cellular actin to propel themselves through the cytoplasm of one cell and into neighbouring cells. The speed at which the bacteria are driven is cell specific varying between 0.02 and 1.5µm/se ...
... microinjection of stable actin nuclei. Recently, it has come to light that certain are capable of using the cellular actin to propel themselves through the cytoplasm of one cell and into neighbouring cells. The speed at which the bacteria are driven is cell specific varying between 0.02 and 1.5µm/se ...
Summary of risk management plan and specific licence conditions
... vigour is greatest in the first generation and declines in subsequent generations. Plant breeders have often used male sterile plants to accomplish hybrid seed production. The male sterile line of InVigor® canola contains the barnase gene from the soil bacterium Bacillus amyloliquefaciens. The barna ...
... vigour is greatest in the first generation and declines in subsequent generations. Plant breeders have often used male sterile plants to accomplish hybrid seed production. The male sterile line of InVigor® canola contains the barnase gene from the soil bacterium Bacillus amyloliquefaciens. The barna ...
Thiazolidinediones and Bone Metabolism
... stimulates insulin receptor substrate-1 (IRS-1) expression Insulin also activates steroidogenic acute regulatory (StAR) protein expression which leads to increased progesterone, testosterone and estrogen synthesis TZDs interact with PPAR-g which in turn affect components of insulin signaling pathway ...
... stimulates insulin receptor substrate-1 (IRS-1) expression Insulin also activates steroidogenic acute regulatory (StAR) protein expression which leads to increased progesterone, testosterone and estrogen synthesis TZDs interact with PPAR-g which in turn affect components of insulin signaling pathway ...
Heavy Metal Detoxification in Plants
... gene (referred to as AtPCS1 ) (15) and a similar gene in wheat (TaPCS1 ) (16) were identi ed by two other groups through the ability of cDNAs to confer resistance to Cd when expressed in the yeast S. cerevisiae. Both of these latter studies used a variety of yeast mutants to demonstrate the mechani ...
... gene (referred to as AtPCS1 ) (15) and a similar gene in wheat (TaPCS1 ) (16) were identi ed by two other groups through the ability of cDNAs to confer resistance to Cd when expressed in the yeast S. cerevisiae. Both of these latter studies used a variety of yeast mutants to demonstrate the mechani ...
Pochonia chlamydosporia - Biological Engineering
... the fungal polyketides, is produced by Pochonia chlamydosporia. It inhibits the Hsp90 molecular chaperone, another important target for cancer chemotherapy. Recently, gene clusters for biosynthesis of radicicol from Pochonia chlamydosporia were sequenced. However, the function of each enzyme is stil ...
... the fungal polyketides, is produced by Pochonia chlamydosporia. It inhibits the Hsp90 molecular chaperone, another important target for cancer chemotherapy. Recently, gene clusters for biosynthesis of radicicol from Pochonia chlamydosporia were sequenced. However, the function of each enzyme is stil ...
Degree of reduction
... Fats serve as polymeric biological fuel storage. In addition, lipids constitute portions of more complex molecules, such as lipopolysaccharides. ...
... Fats serve as polymeric biological fuel storage. In addition, lipids constitute portions of more complex molecules, such as lipopolysaccharides. ...
Structure - chula ise
... Regulatory growth factors, hormones, transcription factors Receptor cell surface receptors ...
... Regulatory growth factors, hormones, transcription factors Receptor cell surface receptors ...
Gene regulatory network
A gene regulatory network or genetic regulatory network (GRN) is a collection of regulators thatinteract with each other and with other substances in the cell to govern the gene expression levels of mRNA and proteins.The regulator can be DNA, RNA, protein and their complex. The interaction can be direct or indirect (through their transcribed RNA or translated protein).In general, each mRNA molecule goes on to make a specific protein (or set of proteins). In some cases this protein will be structural, and will accumulate at the cell membrane or within the cell to give it particular structural properties. In other cases the protein will be an enzyme, i.e., a micro-machine that catalyses a certain reaction, such as the breakdown of a food source or toxin. Some proteins though serve only to activate other genes, and these are the transcription factors that are the main players in regulatory networks or cascades. By binding to the promoter region at the start of other genes they turn them on, initiating the production of another protein, and so on. Some transcription factors are inhibitory.In single-celled organisms, regulatory networks respond to the external environment, optimising the cell at a given time for survival in this environment. Thus a yeast cell, finding itself in a sugar solution, will turn on genes to make enzymes that process the sugar to alcohol. This process, which we associate with wine-making, is how the yeast cell makes its living, gaining energy to multiply, which under normal circumstances would enhance its survival prospects.In multicellular animals the same principle has been put in the service of gene cascades that control body-shape. Each time a cell divides, two cells result which, although they contain the same genome in full, can differ in which genes are turned on and making proteins. Sometimes a 'self-sustaining feedback loop' ensures that a cell maintains its identity and passes it on. Less understood is the mechanism of epigenetics by which chromatin modification may provide cellular memory by blocking or allowing transcription. A major feature of multicellular animals is the use of morphogen gradients, which in effect provide a positioning system that tells a cell where in the body it is, and hence what sort of cell to become. A gene that is turned on in one cell may make a product that leaves the cell and diffuses through adjacent cells, entering them and turning on genes only when it is present above a certain threshold level. These cells are thus induced into a new fate, and may even generate other morphogens that signal back to the original cell. Over longer distances morphogens may use the active process of signal transduction. Such signalling controls embryogenesis, the building of a body plan from scratch through a series of sequential steps. They also control and maintain adult bodies through feedback processes, and the loss of such feedback because of a mutation can be responsible for the cell proliferation that is seen in cancer. In parallel with this process of building structure, the gene cascade turns on genes that make structural proteins that give each cell the physical properties it needs.It has been suggested that, because biological molecular interactions are intrinsically stochastic, gene networks are the result of cellular processes and not their cause (i.e. cellular Darwinism). However, recent experimental evidence has favored the attractor view of cell fates.