Data Mining in Ensembl with BioMart
... BioMart- Data mining • BioMart is a search engine that can find multiple terms and put them into a table format. ...
... BioMart- Data mining • BioMart is a search engine that can find multiple terms and put them into a table format. ...
Chapter 14
... • Mutations (“GENE MISTAKES”) can results from base-pair substitutions, insertions (frameshift mutations), deletion • Results when DNA regions (called transposable elements) move form one location to another in the same DNA molecule of different one ...
... • Mutations (“GENE MISTAKES”) can results from base-pair substitutions, insertions (frameshift mutations), deletion • Results when DNA regions (called transposable elements) move form one location to another in the same DNA molecule of different one ...
Regulation of Gene Expression
... Control of Transcription DNA has “on” and “off” switches Activator –protein that binds near gene’s promoter region - allows RNA polymerase to transcribe (allows it to fit) Repressor – protein that binds to DNA and prevents RNA polymerase from binding -coded for by “regulator” gene ...
... Control of Transcription DNA has “on” and “off” switches Activator –protein that binds near gene’s promoter region - allows RNA polymerase to transcribe (allows it to fit) Repressor – protein that binds to DNA and prevents RNA polymerase from binding -coded for by “regulator” gene ...
Michael Boutros – from the study of social gene networks to the
... Boutros and his colleagues used an innovative high-throughput screening approach for analysing genetic interactions. It enabled a genome-wide identification of genes using RNA interference (RNAi) and meant that the researchers could specifically silence particular genes. By combining this combinator ...
... Boutros and his colleagues used an innovative high-throughput screening approach for analysing genetic interactions. It enabled a genome-wide identification of genes using RNA interference (RNAi) and meant that the researchers could specifically silence particular genes. By combining this combinator ...
Gene Expression/Transcription
... Ronald Ellis, Ph.D. We study the development and evolution of germ cells, using cutting-edge techniques that are have made nematodes a leading model for animal biology. First, we study the control of germ cell fates. Animals must produce sperm or eggs to reproduce. Although these cells differ dramat ...
... Ronald Ellis, Ph.D. We study the development and evolution of germ cells, using cutting-edge techniques that are have made nematodes a leading model for animal biology. First, we study the control of germ cell fates. Animals must produce sperm or eggs to reproduce. Although these cells differ dramat ...
Gene Control
... the activator transcription factors to slow transcription i. by binding to distal control elements and keeping activators out ii. By binding to activator proteins ...
... the activator transcription factors to slow transcription i. by binding to distal control elements and keeping activators out ii. By binding to activator proteins ...
II. Lecture Section 2 CELL SPECIALIZATION: Regulation of
... b. The sequence and chemistry of amino acid side chains gives the protein its shape and the shape gives the protein its function 1. Basic protein characteristic, such as binding selectivity, chemical and enzymatic reactivity and protein conformation, are the result 2. Regulation of Protein Structure ...
... b. The sequence and chemistry of amino acid side chains gives the protein its shape and the shape gives the protein its function 1. Basic protein characteristic, such as binding selectivity, chemical and enzymatic reactivity and protein conformation, are the result 2. Regulation of Protein Structure ...
protein synthesis
... Transcription and translation are the two main processes linking gene to protein • Genes provide the instructions for making specific proteins. • The bridge between DNA and protein synthesis is RNA. • RNA is chemically similar to DNA, except that it contains ribose as its sugar and substitutes the n ...
... Transcription and translation are the two main processes linking gene to protein • Genes provide the instructions for making specific proteins. • The bridge between DNA and protein synthesis is RNA. • RNA is chemically similar to DNA, except that it contains ribose as its sugar and substitutes the n ...
Constructing a Model of Protein Synthesis
... In a process called transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNA’s nucleotide sequences in the form of a complementary RNA molecule. Then the mRNA carries this information in the form of a code to the ribosomes, where protein synthesis ta ...
... In a process called transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNA’s nucleotide sequences in the form of a complementary RNA molecule. Then the mRNA carries this information in the form of a code to the ribosomes, where protein synthesis ta ...
Finding Disease Genes
... finally found one 15 cM from CF gene on chromosome 7. More work led to tightly linked markers D7S8 and met (oncogene) flanking it--2 Mbp apart. (as as later realized) no known deletion or rearrangement causes CF. walking by cosmids over 500 kbp problem with uncloneable repeated sequences: needed to ...
... finally found one 15 cM from CF gene on chromosome 7. More work led to tightly linked markers D7S8 and met (oncogene) flanking it--2 Mbp apart. (as as later realized) no known deletion or rearrangement causes CF. walking by cosmids over 500 kbp problem with uncloneable repeated sequences: needed to ...
Network models - Department of Computing
... Edges are added between pairs of nodes uniformly at random with the same probability p Two (equivalent) methods for constructing ER graphs: ...
... Edges are added between pairs of nodes uniformly at random with the same probability p Two (equivalent) methods for constructing ER graphs: ...
Presentation (PowerPoint File) - IPAM
... a PE and a PPE protein: express complex, and determine its structure Research of Shuishu Wang and Michael Strong ...
... a PE and a PPE protein: express complex, and determine its structure Research of Shuishu Wang and Michael Strong ...
MUTUAL INFORMATION-BASED SUPERVISED ATTRIBUTE
... 2.2 Pre-processing of gene expression data A microarray experiment typically assesses a large number of DNA sequences under multiple conditions. These conditions may be a time series during a biological process or a collection of different tissue samples. Focus on the cluster analysis of gene expres ...
... 2.2 Pre-processing of gene expression data A microarray experiment typically assesses a large number of DNA sequences under multiple conditions. These conditions may be a time series during a biological process or a collection of different tissue samples. Focus on the cluster analysis of gene expres ...
Chapter 8: The Origins of Life
... Rather than originating in a single event, life may have arisen through a series of steps, each building upon the complexity established by the previous mechanism During its evolution, life may have undergone evolutionary selection for certain metabolic pathways over others o Some of these pathw ...
... Rather than originating in a single event, life may have arisen through a series of steps, each building upon the complexity established by the previous mechanism During its evolution, life may have undergone evolutionary selection for certain metabolic pathways over others o Some of these pathw ...
Bioproduction of recombinant protein
... transduction (Rate of transduced cells and fluorescent intensity level) using concentrated GFP lentiviral suspension. Secondly, protein of interest is produced using cDNA-His tagged-expressing lentiviral vectors, the supernatant is harvested and western blot analysis performed. ...
... transduction (Rate of transduced cells and fluorescent intensity level) using concentrated GFP lentiviral suspension. Secondly, protein of interest is produced using cDNA-His tagged-expressing lentiviral vectors, the supernatant is harvested and western blot analysis performed. ...
ap biology syllabus
... *I CAN describe the basic structure and function of DNA, mRNA, amino acids, polypeptides, and replication (e.g. replication, transcription, and translation). *I CAN describe the experiments of major scientists in determining both the structure and central dogma of DNA. *I CAN use mRNA codon charts t ...
... *I CAN describe the basic structure and function of DNA, mRNA, amino acids, polypeptides, and replication (e.g. replication, transcription, and translation). *I CAN describe the experiments of major scientists in determining both the structure and central dogma of DNA. *I CAN use mRNA codon charts t ...
Outline
... Test it in cells • RBP and GBP involved in signal transduction pathways at the gene levels • presence of ligand to the above mentioned receptors will cause the upregulation of b-galactosidase gene expression. • The RBP and GBP were replaced with the designed TNT and L-lactate receptors and tested ...
... Test it in cells • RBP and GBP involved in signal transduction pathways at the gene levels • presence of ligand to the above mentioned receptors will cause the upregulation of b-galactosidase gene expression. • The RBP and GBP were replaced with the designed TNT and L-lactate receptors and tested ...
TIGR_ISS
... Visually inspect alignments, look for conserved active sites, look for (generally) at least 35% identity across the full lengths of both proteins. If matches are not full length, look to see if there are recognized functional domains in the area where the match occurs. Decide how much information ca ...
... Visually inspect alignments, look for conserved active sites, look for (generally) at least 35% identity across the full lengths of both proteins. If matches are not full length, look to see if there are recognized functional domains in the area where the match occurs. Decide how much information ca ...
F - 8th International Biocuration Conference
... • Network analysis indicated that the other candidate hypoxia-response genes we identified would all be regulated by HIF signaling pathway, suggesting an essential role of EPAS1 in the adaption of high-altitude dogs. • Interestingly, EPAS1 was also identified as a selective target in Tibetan people. ...
... • Network analysis indicated that the other candidate hypoxia-response genes we identified would all be regulated by HIF signaling pathway, suggesting an essential role of EPAS1 in the adaption of high-altitude dogs. • Interestingly, EPAS1 was also identified as a selective target in Tibetan people. ...
Skills Worksheet
... In the space provided, write the letter of the description that best matches each term . ...
... In the space provided, write the letter of the description that best matches each term . ...
Gene regulatory network
A gene regulatory network or genetic regulatory network (GRN) is a collection of regulators thatinteract with each other and with other substances in the cell to govern the gene expression levels of mRNA and proteins.The regulator can be DNA, RNA, protein and their complex. The interaction can be direct or indirect (through their transcribed RNA or translated protein).In general, each mRNA molecule goes on to make a specific protein (or set of proteins). In some cases this protein will be structural, and will accumulate at the cell membrane or within the cell to give it particular structural properties. In other cases the protein will be an enzyme, i.e., a micro-machine that catalyses a certain reaction, such as the breakdown of a food source or toxin. Some proteins though serve only to activate other genes, and these are the transcription factors that are the main players in regulatory networks or cascades. By binding to the promoter region at the start of other genes they turn them on, initiating the production of another protein, and so on. Some transcription factors are inhibitory.In single-celled organisms, regulatory networks respond to the external environment, optimising the cell at a given time for survival in this environment. Thus a yeast cell, finding itself in a sugar solution, will turn on genes to make enzymes that process the sugar to alcohol. This process, which we associate with wine-making, is how the yeast cell makes its living, gaining energy to multiply, which under normal circumstances would enhance its survival prospects.In multicellular animals the same principle has been put in the service of gene cascades that control body-shape. Each time a cell divides, two cells result which, although they contain the same genome in full, can differ in which genes are turned on and making proteins. Sometimes a 'self-sustaining feedback loop' ensures that a cell maintains its identity and passes it on. Less understood is the mechanism of epigenetics by which chromatin modification may provide cellular memory by blocking or allowing transcription. A major feature of multicellular animals is the use of morphogen gradients, which in effect provide a positioning system that tells a cell where in the body it is, and hence what sort of cell to become. A gene that is turned on in one cell may make a product that leaves the cell and diffuses through adjacent cells, entering them and turning on genes only when it is present above a certain threshold level. These cells are thus induced into a new fate, and may even generate other morphogens that signal back to the original cell. Over longer distances morphogens may use the active process of signal transduction. Such signalling controls embryogenesis, the building of a body plan from scratch through a series of sequential steps. They also control and maintain adult bodies through feedback processes, and the loss of such feedback because of a mutation can be responsible for the cell proliferation that is seen in cancer. In parallel with this process of building structure, the gene cascade turns on genes that make structural proteins that give each cell the physical properties it needs.It has been suggested that, because biological molecular interactions are intrinsically stochastic, gene networks are the result of cellular processes and not their cause (i.e. cellular Darwinism). However, recent experimental evidence has favored the attractor view of cell fates.