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Introduction to flaviviral envelope glycoprotein E
Introduction to flaviviral envelope glycoprotein E

... the class II viral envelope proteins from flaviviruses and alphaviruses are very similar in the structural organisation of the domains and subunits [Bressanelli et al. 2004; Gibbons et al. 2004; Kuhn et al. 2002; Modis et al. 2004; Zhang et al. 2003, 2002]. The pH threshold for fusion depends on the ...
Chapter 19 - HCC Learning Web
Chapter 19 - HCC Learning Web

... They are infectious particles consisting of nucleic acid encased in a protein coat, and possibly, a membranous envelope. Viruses range in size from only 20nm in diameter to that barely resolvable with a light ...
̚Ꮈ̂ጯ Ⴧ̀঱٢Ϡ  ྏྏᗟ
̚Ꮈ̂ጯ Ⴧ̀঱٢Ϡ ྏྏᗟ

... (1) If you want to prepare the primer for the concentration of 30 p mol/µl, please calculate how much water you will need to add into the primer? (2) If you want to prepare the primer for the concentration of 10 µM, please calculate how much water you will need to add? (3) Since the PCR product cont ...
English Version
English Version

... center, E-S complex, zymogen, Km, Vmax. 2. Familiarity with enzyme specificities, effects of pH and temperature on rates of enzymatic reactions, differences of competitive inhibition, noncompetitive inhibition, uncompetitive inhibitions, and allosteric regulatory of enzyme. 3. To know the mechanism ...
Ten novel interaction partners for the histone H2A protein
Ten novel interaction partners for the histone H2A protein

... Database (genome-www.stanford.edu/Saccharomyces) to check for sequence identity with known genes. The sequence identity from one clone (S12) could not be determined. S9 expressed a protein that was a known interactor with Hta1. Out of the remaining 11 clones, S6 and S11 expressed the same protein. T ...
Exam II Review: - Texas Tech University
Exam II Review: - Texas Tech University

... the 3’ end of the intron, forming a phosphodiester bond with the 5’ terminal phosphate of the 3’ exon, yielding the spliced product. The intronic lariat is released with its 3’ OH group and is rapidly recycled. ...
NUTRITIONAL REGULATIN OF GROWTH
NUTRITIONAL REGULATIN OF GROWTH

... If fed a high level of nutrition late in production after being deprived of nutrients will deposit more fat If fed a low level of nutrition late in production, then they will produce leaner carcasses Figure 11.9 and table 11.6 & 7 ...
Microbiology
Microbiology

... A 517 bp DNA fragment from the PurE gene was amplified, cloned into pDIA5304 and integrated into B. stibtilis 168. Chromosomal DNA isolated from four transformants was restricted with EcoRI. The religated DNA was transformed into E . coli TP611. Only one chloramphenicol-resistant clone containing pl ...
6 Characterization of Casein and Bovine Serum Albumin (BSA)
6 Characterization of Casein and Bovine Serum Albumin (BSA)

... physical characteristics of a protein can be used to isolate it from other cellular components using basic chemical techniques. Once a protein has been isolated, one can initiate characterization studies, information such as pH- and heat-stability, that could prove helpful in ascertaining the protei ...
Why cooking of dog food can cause allergies and
Why cooking of dog food can cause allergies and

... Heating protein - breaking the structure down In a way, this enzyme breakdown process is similar to what happens when you heat a protein. Heat generally means, "supplying molecular energy". The protein molecules absorb this energy by moving. They shake and rotate. The more heat, the faster they move ...
12866_2017_1009_MOESM5_ESM
12866_2017_1009_MOESM5_ESM

... [ref 1] Nakamura S, Sato H, Tanaka R, Yaguchi T: Verification of Ribosomal Proteins of Aspergillus fumigatus for use as Biomarkers in MALDI-TOF MS identification. Mass Spectrometry (Tokyo) 2016, 5:A0049. [ref 2] Varshavsky A: The N-end rule: Functions, mysteries, uses. Proc. Natl. Acad. Sci. USA, 19 ...
Todd Eckdahl - Davidson College
Todd Eckdahl - Davidson College

... Minor Groove Binding Drugs  Anti-tumor properties  Conformational change in the 3D structure of DNA  Prior Knowledge of MGBD/DNA interaction  As models for minor groove binding proteins ...
Medical School Biochemistry
Medical School Biochemistry

... Alternate splicing of tropomyosin gene transcripts can generate a family of tissuespecific proteins In intestinal cells, the apoprotein B mRNA is modified creating a protein of smaller size than its liver counterpart During the production of antibodies, lymphocytes produce shorter transcripts by usi ...
3D modelling activity
3D modelling activity

... still essentially the same (though vastly easier). These models must be confirmed using a process called x-ray crystallography. This is based on the scatter of x-rays by a pure crystal of the protein of interest. Unfortunately the process of generating x-ray crystallographic images of a molecule is ...
Metabolism
Metabolism

... Pyruvate ...
Chem 464 Biochemistry
Chem 464 Biochemistry

... has many negatively charged phosphate groups. The pI of histones is very high, about 10.8, What amino acid residues must be present in relatively large numbers in histones? In what way do these residues contribute to the strong binding of histones to DNA? If the histones have a high pI, they must be ...
ASAHL antibody - middle region (ARP44939_P050)
ASAHL antibody - middle region (ARP44939_P050)

... Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. ...
Protein basics - Crop Genebank Knowledge Base
Protein basics - Crop Genebank Knowledge Base

... Transport (oxygen transfer with haemoglobin) Messengers (insulin and certain other hormones) Antibodies (proteins that bind to specific foreign particles) Regulation (proteins involved in regulating DNA synthesis) ...
1.4+ billion cows X 200 liters of methane per day = > 7 million tons of
1.4+ billion cows X 200 liters of methane per day = > 7 million tons of

... Why are proteins not completely flexible? Why are proteins not completely stiff? ...
CH 6: Proteins and Amino Acids
CH 6: Proteins and Amino Acids

... – Liver disease – Inadequate protein/food intake – Kidney disease  blood proteins excreted – Injury breaks open cells ...
MSc in Biochemistry Dissertation Project – 2nd Cycle Student´s
MSc in Biochemistry Dissertation Project – 2nd Cycle Student´s

... Staphylococcus aureus is a remarkably versatile microorganism that is usually a commensal of the human’s microbiota, but can also become invasive when the host’s defenses are breached, becoming a very important and successful pathogen, with high morbidity and mortality rates across the globe. In S. ...
What happens to proteins key
What happens to proteins key

... Each cell contains DNA for making every protein in the body, but each cell does not make them all. ...
Quaternary structures
Quaternary structures

... E.g.: tubulin, actin, TMV coat ...
1. Proteins Are Informational and Functional Biological Polymers
1. Proteins Are Informational and Functional Biological Polymers

... encode proteins involved in a given function - is well known. For example, DNA sequences coding for the proteins involved in the synthesis of lactose are positioned close together in the so-called lac operon. It has been further postulated that proteins that interact are likewise closely placed in t ...
Protein: Amino Acids
Protein: Amino Acids

... Protein • Essential part of all living cells • Found in animals and plants; Particularly rich in animal foods – meats, poultry, eggs, etc. • Protein is in your skin, hair, nails, muscles, etc. • Made of amino acids – amino acids contain nitrogen, which is necessary for life. ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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